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Citations for Becton, Dickinson and Company :
6451 - 6500 of 6817 citations for 1 Hydroxymethyl benzo cd indol 2 1H one since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... and then incubated overnight with hamster anti-CD3ε antibody diluted in 1% goat serum/0.3% Triton-X100/TBS (BD Biosciences, 550277). The next day samples were washed in TBS and incubated at room temperature in goat anti-hamster 647 (Life Technologies ...
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bioRxiv - Microbiology 2023Quote: ... pH 7.2) or BS-N buffer supplemented with 1% casein hydrolysate (casamino acids technical grade; Becton, Dickinson and Company, NJ, USA) at an OD660 of 4.0 was transferred to a ProteoSave SS 1.5-mL centrifuge tube (Sumitomo Bakelite ...
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bioRxiv - Cell Biology 2024Quote: ... and screened for protein levels via Western blots with monoclonal mouse anti-IQGAP1 (1:1000; 610612, BD Biosciences, East Rutherford, NJ), polyclonal rabbit α-tubulin (1:2500 ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were stained for 30 min at 4 °C and washed with cell staining buffer and acquired within 1 h on a custom SORP five laser FACSAria Fusion (BD Biosciences).
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bioRxiv - Cell Biology 2023Quote: Both WT and CD13KO mice of both genders were intraperitoneally injected with 1 mL 4% Brewers Thioglycollate (TG) solution (BD; 211716). After 48 hours mice were then euthanized via CO2 and cervical dislocation and injected with 10 mL cold PBS into the peritoneal cavity to collect peritoneal macrophages ...
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bioRxiv - Neuroscience 2023Quote: ... aristae or the third segment of antennae were surgically removed by a needle (BD PrecisionGlide Hypodermic Needles 18G x 1” BD 305195) connected to a syringe.
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bioRxiv - Immunology 2023Quote: ... and 1:20 dilution mouse anti-human CD123 (IL-3)-PE (stock 200 ug/mL, BD Biosciences, Franklin Lakes, NJ, USA). A 1:1,000 dilution of anti-mouse IgG Alexa-647 (stock 1 mg/mL ...
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bioRxiv - Microbiology 2023Quote: ... The resulting tissue homogenates were each serially diluted from 10-1 to 10-7 and plated in triplicates onto Marine Agar 2216 (MA, BD Difco). Following one week of incubation at 23 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... and either 1×106 VCaP or 5×105 22Rv1 cells suspended in 100 μl of PBS with 50% Matrigel (BD Biosciences) were implanted subcutaneously into the dorsal flank on both sides of the mice ...
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bioRxiv - Immunology 2023Quote: ... Single cells were then washed with 1% Bovine serum albumin (BSA) in PBS and blocked with anti-Fcγ receptor (BD Biosciences). Human peripheral blood mononuclear cells (PBMC ...
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bioRxiv - Cell Biology 2023Quote: ... The cells followed by an incubation with the SM/Chol probe OlyA (3 µM) and primary anti-IFN-γR1 antibody (1:50) (BD Biosciences) together in 1% BSA-PBS at room temperature for 90 min ...
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bioRxiv - Cancer Biology 2023Quote: ... After washing the slides were blocked for 30 min in Superblock solution following the incubation with the primary antibodies mouse anti-BrdU/IdU (1:100, BD Bioscscience) and rat anti-BRDU/CIdU (1:200 ...
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bioRxiv - Cancer Biology 2023Quote: ... and total DNA (7-AAD 1:50 solution dilution) and analyzed by flow cytometer in a BD LSRFortessa cell analyzer (BD Biosciences) using 488 nm and 640 nm lasers ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed twice in PBS-FBS 1% and stained with 20 µL of anti Ki-67 (FITC Mouse Anti-Ki-67 Set, BD Biosciences) for 30 min at RT in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... Stained cells were analyzed and 7AAD−Lin−Vcam−Sca1+ (Stem cell antigen 1; Ly6a) (FAPs) were isolated using FACS Aria III cell sorter (BD Biosciences) with 4-way-purity precision ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed two times with 150 µL PBS (centrifuged 5 min at 400 × g at 4 °C) and resuspended in 50 µL Fc-block (1:50, Purified Rat Anti-Mouse CD16/CD32, BD, #553142) diluted in FACS buffer (1x PBS/2% FBS/2mM ...
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bioRxiv - Immunology 2023Quote: ... Effector cells were incubated with K562 and Nalm6 variants for 4 hours at a ratio of 5:1 with addition of Brefeldin A (GolgiPlug, BD Biosciences). Next ...
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bioRxiv - Cancer Biology 2023Quote: ... per well of U-bottom plate and coincubated for 4h at 37°C in the presence of NH4Cl and 1 µl Golgi PLUG (BD Biosciences). After that time cells were washed three times with 200 µl PBS and centrifuged (5 min ...
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bioRxiv - Cancer Biology 2023Quote: ... CD8/CD4 T cells ratio and activation was assessed with BD Multitest CD3/CD8/CD45/CD4 (1:20; BD Biosciences, #342317) and APC-CD69 (1:100 ...
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bioRxiv - Neuroscience 2022Quote: ... the cell suspension was then incubated on two PDGFRα plates coated with rat anti-PDGFRα IgG (BD Biosciences, 558774; 1:500). The adherent cells were washed with PBS to remove all antigen-negative nonadherent cells ...
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bioRxiv - Neuroscience 2022Quote: ... cells were fixed with IC fix (eBioscience88-8824-00) for 30 minutes followed by incubation with CD74 FITC 1:100 (BD 561941) in permeabilization buffer (eBioscience88-8824-00 ...
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bioRxiv - Cell Biology 2022Quote: Freshly dissected LW whole mount preparations from 6 wild-type and 4 Lrp2-/267 mice were incubated with rat anti-CD24 conjugated with Phycoerythrin (PE; 1:100; BD Pharmingen) in Neurobasal medium (Gibco ...
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bioRxiv - Cancer Biology 2022Quote: The fraction of CD44+/CD24− cells was determined by trypsinizing and resuspending cells in FACS buffer at 10×106 cells/mL followed by incubation with antibodies against human CD44 (APC-conjugated, Clone G44-26, 1:5, BD Biosciences) and CD24 (PE-Cy7-conjugated ...
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bioRxiv - Cell Biology 2022Quote: For 3T3 NIH fibroblasts cells infected with C/EBPα-ER-IRES-hCD4 (WT and T35A) were incubated with 0.1µg per 1 million cells of both Fc block and hCD4-Biotin antibody (BD Pharmingen, Cat#555347) for 10 and 20 minutes respectively ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then washed twice with 1× PBS and flow cytometry analysis was performed on a FACS Canto II flow cytometer (BD Biosciences); data analysis was carried out using FlowJo software (BD Biosciences).
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bioRxiv - Immunology 2022Quote: ... were re-stimulated in vitro for 5h at 37°C 5% CO2 in cRPMI supplemented with GolgiStop (1/250; BD bioscience), GolgiPlug (1/500 ...
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bioRxiv - Immunology 2022Quote: ... cultured splenocytes were resuspended in 40 μL FACS buffer containing fluorochrome-conjugated antibodies and incubated 1 hr at 4 °C followed by cell fixation using Cytofix/Cytoperm buffer (BD Bioscience) for 20 min at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... was added and 0.6-1×106 lymphocyte-gated events were immediately acquired on an LSRII SORP (BD, San Diego, CA, USA).
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bioRxiv - Immunology 2022Quote: ... ELISAs were performed using paired rat anti-mouse IgE antibodies (capture with 1 μg/mL purified R35-72, BD Biosciences, detect with biotinylated R35-118, BD Biosciences) and streptavidin-HRP (BD Biosciences) ...
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bioRxiv - Immunology 2022Quote: ... and others were fixed using PBS containing 1% paraformaldehyde (Labsynth, Diadema, SP, Brazil) and acquired in FACS Canto II (BD Biosciences). Analyses were performed in FlowJo software (Becton Dickinson and Company).
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bioRxiv - Microbiology 2022Quote: ... xanthus cells were grown in liquid CYE medium (10 mM MOPS pH 7.6, 1% (w/v) Bacto™ casitone (BD Biosciences), 0.5% yeast extract and 8 mM MgSO4 ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated antibody bound to RBD in the presence of competitor was stained with Streptavidin-PE at a 1:1000 dilution (BD Biosciences) and analysed on a 200 Bioplex instruments ...
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bioRxiv - Microbiology 2022Quote: ... 50 μL of DEs were diluted into 500 μL of FACS diluent buffer (PBS + 1% Tween-20) in a 5 mL 12 × 75 mm round bottom FACS tube (BD Biosciences). After standard autocalibration on the Sony SH800 ...
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bioRxiv - Neuroscience 2022Quote: ... The resulting pellet was resuspended in 1 ml of complete neurosphere medium containing epidermal growth factor (EGF, 20 ng/ml; receptor grade, BD Biosciences) and basic fibroblast growth factor (bFGF ...
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bioRxiv - Neuroscience 2023Quote: ... and cells were washed and labeled with the following antibodies for 30 min at 4 °C: rat anti-CD8 APC (1:100, catalog no. 553035; BD Biosciences); rat anti-CD45 PE (1:100 ...
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bioRxiv - Immunology 2022Quote: ... cells were stained for p-STAT1 (clone 4alpha) or p-STAT3 (clone 4P/STAT3) for 1 hour (all from BD Biosciences) and were acquired on FACS LSRFortessa flow cytometer (BD Biosciences).
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bioRxiv - Immunology 2022Quote: ... Data acquisition and analysis: 0.5-1×106 events were collected on a BD LSR Fortessa X-50 flow cytometer (BD Immunocytometry Systems). Electronic compensation was performed with antibody capture beads (BD Biosciences) ...
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bioRxiv - Immunology 2022Quote: ... GFP expression in peripheral blood B and T cells was screened using anti-CD19-PE (BD, clone 1D3, dilution 1/100), anti-CD3e-PerCP-Cy5.5 (eBioscience ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed five times and developed with tetramethylbenzidine (1-Step Ultra TMB-ELISA Substrate Solution, Thermo Fisher Scientific, for the RBD ELISA, and BD OptEIA Substrate Solution ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were resuspended in staining buffer consisting of 50% PBS containing 1% bovine serum albumin (BSA) and 50% Brilliant Stain Buffer (BD Biosciences) and incubated for 20 minutes at 4°C in the following antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... 100 μl of this suspension (1×10e5) was transferred to a 5 ml culture tube and 5 μl of FITC Annexin V (BD Biosciences) added ...
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bioRxiv - Evolutionary Biology 2023Quote: ... then the resulting 350 μL volume was drawn twice into a 1 mL luer slip tip tuberculin syringe (BD Catalog # 309659) fitted with a 27G-½” needle ...
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bioRxiv - Developmental Biology 2023Quote: ... or (ii) PE-Annexin V conjugated antibody (1:33 dilution in TBST) and 7-AAD as per kit instructions (BD Pharmingen). Samples were analysed by flow cytometry within 30 min.
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bioRxiv - Developmental Biology 2023Quote: ... and then resuspended in 100 μL Annexin V binding buffer with either (i) FITC-Annexin V conjugated antibody (1:33 dilution in TBST) and BD propidium iodide staining solution (BD Pharmingen) or (ii ...
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bioRxiv - Immunology 2023Quote: ... Single cell suspensions of ex vivo organs were co-cultured with DCs in complete RMPI at a ratio of approximately 10 T cells to 1 DC in the presence of Golgi Plug (BD Bioscience). Co-cultures were incubated at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... HC-04 cells were washed and fixed with 1% PFA in PBS before quantifying dextran-positive cells by flow cytometry (LSR II instrument, BD Biosciences). For data analysis using FlowJo V.10.0.8 (Tree Star) ...
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bioRxiv - Cancer Biology 2023Quote: ... mice bone marrow mononuclear cells were first stained with lineage (Lin) cocktail containing biotinylated antibodies against Ly-6G/Ly-6C (Gr-1) Biotin RB6-8C5 (BD Bioscience) (1:100) ...
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bioRxiv - Immunology 2022Quote: ... Single-cell suspensions were incubated with Fc block (anti-mouse CD16/CD32, 1 μg for 1x106 cells, BD Biosciences, Cat# 553142) at 4°C for 30 min ...
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bioRxiv - Physiology 2023Quote: ... cells were incubated at 4°C for 30 min with APC-conjugated rat anti-mouse CD31 (1:200, BD Pharmigen, 551262). DAPI (5 mg/ml ...
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bioRxiv - Microbiology 2024Quote: ... Cells were activated for 3 days with 1 μg/mL/million cells of purified NA/LE mouse anti-human CD3 and CD28 (BD Biosciences) and infected with HIVBaL at 0.01 ng/106 cells ...