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Citations for Becton, Dickinson and Company :
6401 - 6450 of 6817 citations for 1 Hydroxymethyl benzo cd indol 2 1H one since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... and cell surface markers were stained with the following anti-mouse antibodies at 1:200 dilution per 106 cells: Ly6C (AL-21; BD Biosciences), Ly6G (1A8 ...
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bioRxiv - Immunology 2020Quote: ... spleen or lymph node cells in complete RMPI at a ratio of approximately 10 T cells to 1 DC in the presence of Golgi Plug (BD Bioscience). Co-cultures were incubated at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... Sections were stained using the following antibodies: rat anti-mouse Ly-6C/Ly-6G (RB6-8C5, BD Bioscience, #550291, 1/200) followed by AlexaFluor647-conjugated goat anti-rat IgG (ThermoFisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... or with no target cells (or an E:T of 1:0) and cultured overnight before being staining with allophycocyanin (APC)-conjugated anti human-CD69 antibody (BD Biosciences #555533). Flow cytometry was performed using a BD- Fortessa (BD Biosciences ...
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bioRxiv - Bioengineering 2021Quote: ... To express CRE and induce recombination phleomycin resistant colonies were selected and grown overnight in 3ml of YP-Raffinose (1% w/v yeast extract (BD Biosciences), 2% w/v Bacto-peptone (BD Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... splenocytes and tumor-infiltrating cells were cocultured with 1 μmol/L of AdpgkMut or AH1 peptide in the presence of Brefeldin A (BD Biosciences) for 5h in vitro ...
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bioRxiv - Immunology 2020Quote: For data acquisition on the BD FACSymphony the following amounts of 14 different fluorescent streptavidin conjugates were added to 10 μl of pMHC monomer (100 μg/ml): 1 μl of SA-BB790 (BD, custom), 1 μl of SA-BB630 (BD ...
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bioRxiv - Immunology 2021Quote: ... Resting- or in vitro-generated effector CD4+ or CD8+ T cells (105 cells/well) were stimulated with 1 μg/ml plate-bound anti-mouse CD3 mAb (BD Bioscience) in combination with different concentrations of P3CSK4 ...
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bioRxiv - Microbiology 2021Quote: ... Escherichia coli strains were routinely grown aerobically at 37 °C under aerobic conditions with shaking at 1 × g in Luria-Bertani medium (BD, USA), supplemented with 25 μg mL−1 chloramphenicol (Merck ...
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bioRxiv - Immunology 2021Quote: ... infarct macrophages as CD45+/CD11b+/Ly6G−/F4/80+ cells and infarct neutrophils as CD45+/CD11b+/F4/80−/Ly6G+ cells.7–9 The following antibodies were used: anti-CD45 (clone 104, 1:100, BioLegend/BD Biosciences); anti-F4/80 (clone BM8 ...
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bioRxiv - Immunology 2021Quote: ... The cells were washed two times and resuspended in 1% paraformaldehyde/1x PBS for collection on a LSR II or FACSymphony flow cytometer (BD Biosciences). Data was analyzed in Flow Jo version 9.9.4.
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bioRxiv - Molecular Biology 2022Quote: ... Cells were washed and resuspended in 1% BSA with DAPI and detected by flow cytometry on a BD LSRFORTESSA X-20 (BD Biosciences). Data analysis was done using FlowJo V10.
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bioRxiv - Neuroscience 2023Quote: ... all samples were filtered through a 70-μm nylon mesh and blocked with anti-Fc CD16/32 (1:40; BD Biosciences). For FOXP3 staining ...
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bioRxiv - Immunology 2022Quote: ... The permeabilized cells were stained with intracellular cytokine staining antibodies for 20 min at room temperature in 1× perm/wash buffer (BD Biosciences). Cells were then washed twice with perm/wash buffer and once with staining buffer before acquisition using the BD Symphony Flow Cytometer and the associated BD FACS Diva software ...
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bioRxiv - Immunology 2023Quote: ... Functionalised agarose beads were washed twice and then incubated with 20 nM of the GFP-streptavidin tetramers and anti-mouse IgG2a/b BV421 antibody (1:10 dilution, clone R2-40, BD Bioscience) for 30 min on ice ...
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bioRxiv - Cancer Biology 2023Quote: ... tumor or normal organ tissue slides were incubated with primary antibody of Ki-67 (1:10000 dilution; Cat# 550609, BD Biosciences), p-Abi-1-S323 (1:500 dilution ...
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bioRxiv - Bioengineering 2023Quote: ... Gates were determined using the isotype controls mouse anti-IgG2b κ (APC-conjugated, Clone 27-35, 1:5, BD Biosciences, US) and mouse anti-IgG2a κ (PE-Cy7-conjugated ...
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bioRxiv - Developmental Biology 2023Quote: ... and stained with conjugated antibodies for 1 hour on ice: mouse anti-C-peptide antibody conjugated with Alexa Fluor 647 (BD Biosciences) and mouse anti-glucagon antibody conjugated with Bv421 (BD Biosciences) ...
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bioRxiv - Biophysics 2022Quote: ... samples were first resuspended in 15.5 μL PBS/0.1% adBSA and incubated with 0.5 μg of rat anti-mouse CD9-PE (Clone: KMC8, IgG2a, κ lot 7268877, BD Biosciences) or matched isotype antibodies (Rat IgG2a ...
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bioRxiv - Cell Biology 2023Quote: ... NTHi was inoculated into 50 ml brain-heart-infusion broth (Roth, Germany) with 1% supplement B (BD Difco™; BD, Germany) and incubated overnight at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... iPSC were detached and singularised using EDTA solution (0.5 M EDTA/PBS) and replated on Matrigel (1:120, BD Bioscience, #354230) at a density of 8,000-12,000 cells/cm2 ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed in 3ml FACS buffer before being fixed in 1% paraformaldehyde and analyzed within 5 days using a Fortessa Flow Cytometer and BD FACSDiva Software (BD Biosciences).
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bioRxiv - Genetics 2023Quote: ... and CD43-B cells were cultured at a density of 5×105 cells per ml in RPMI medium supplemented with 15% FBS and 25 ng ml−1 of IL-4 (R&D) and anti-CD40 (BD Biosciences). Cultured cells were maintained daily at a density of 0.5-1×106 cells per ml ...
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bioRxiv - Cell Biology 2023Quote: ... NTHi was inoculated into 50 ml brain-heart-infusion broth (Roth, Germany) with 1% supplement B (BD Difco™; BD, Germany) and incubated overnight at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 x 106 dissociated organoid cells in 100 µl hepatocyte culture medium and 5% Matrigel were injected subcutaneously into the flank using a 1 ml syringe with 25G needle (BD 305122). For human grafts ...
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bioRxiv - Biochemistry 2023Quote: ... the cells were resuspended with 500 μl PBS containing 1% BSA for detection in BD Accuri C6 flow cytometer system (BD Biosciences) at excitation 488 nm and emission 519 nm ...
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bioRxiv - Systems Biology 2023Quote: ... all transformants were cultured individually in 96-well plates in 1 ml of the appropriate minimal liquid media (AD: -Leu; BD: -Trp) at 30°C until saturated (∼36-48 hours) ...
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bioRxiv - Immunology 2023Quote: ... Bacteria were subcultured from the chocolate agar into brain heart infusion (BHI) broth supplemented with 1% BBL IsoVitaleX (BD Biosciences, # 211875) to a starting OD600=0.1 ...
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bioRxiv - Cell Biology 2023Quote: ... PECAM was stained 1:200 with the live labeling antibody Alexa Fluor® 647 Mouse Anti-Human CD31 (BD Pharmingen™), 2 min prior to imaging ...
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bioRxiv - Immunology 2023Quote: ... single cell suspensions were incubated with 1 µg/ml SIINFEKL peptides in complete RPMI medium for 1 h at 37°C followed by brefeldin A (Golgiplug; BD Pharmingen) addition and further 5 h incubation ...
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bioRxiv - Immunology 2023Quote: 50 × 103 effector cells were cultured alone or together with 5 × 103 tumour cells for 4 h at a 10:1 ratio in the presence of anti-CD107a-PE mAb (clone: H4A3; BD Biosciences) and monensin (3 μM ...
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bioRxiv - Immunology 2023Quote: ... and CD4+FYP+ Treg cells at indicated Treg : Tresp ratios in the presence of 1 μg/ml anti-CD3 antibody (BD Pharmingen). After 3 days ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were incubated with 300 µg/mL DNase for 1 hour at 37°C to expose incorporated brdU and then stained with an APC anti-brdU antibody (1:50, BD Biosciences). 1X BD Perm/Wash in ddH2O was used as a staining and washing buffer ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then resuspended in BD Perm/wash buffer with the following antibodies: BV650 IL4 (1:50, BD Bioscience, cat#564004), APC-Fire750 IFNγ (1:80 ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were submitted to the same procedure as for flow cytometry analysis but without 1% PFA treatment and processed using BD FACSAria (BD Biosciences).
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bioRxiv - Molecular Biology 2022Quote: ... Confluent definitive endoderm cells are then passed 1:3 in presence of Rock Inhibitor on growth factors reduced Matrigel (BD Biosciences) and cultured for 8 days in differentiation medium (DMEM F12 ...
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bioRxiv - Cancer Biology 2022Quote: ... Tumors were engrafted in NSG mice by subcutaneous injection of 1 × 106 cells of H2081 in RPMI1640 medium supplemented with 50% Matrigel (BD Biosciences). Ten days after the injection ...
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bioRxiv - Biochemistry 2022Quote: ... The cells were stained with anti-HbF APC antibody (dilution 1:50) and was acquired and analysed using Cytoflex LX Flow Cytometer (Beckmann Coulter) or AriaIII flow cytometer (BD Biosciences) and analysed using FlowJo (BD Biosciences) ...
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bioRxiv - Developmental Biology 2022Quote: ... Primordial germ cells were purified based on the expression of Zombie Red−/SSEA-1+/CD61+ using BD FACS Aria II Cell Sorter (BD Bioscience). Somatic gonad cells were purified based on the expression of Zombie Red−/SSEA-1−/CD61 ...
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bioRxiv - Microbiology 2022Quote: ... or the TAPI (prepared as described above) was administered directly into the pocket via a 1 mL syringe (BD, cat# 309628). The implants ...
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bioRxiv - Cell Biology 2022Quote: ... 1 % bovine serum albumin (BSA, Jackson, cat. no. 001-000-162) and 10% Perm/Wash buffer (BD Biosciences, cat. no. 554714) at RT for 1 hour ...
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bioRxiv - Cell Biology 2024Quote: ... organoids were dissociated and mCherry positive cells (marking successful electroporation of the pCAS9-mCherry-Frame+1 plasmid) were sorted on ARIAIII sorter (BD Biosciences) and seeded in Matrigel with normal organoid media supplemented with rock inhibitor Y-27632 (10 μM) ...
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bioRxiv - Microbiology 2024Quote: ... Sputum samples were diluted in 1% proteose peptone and then plated on oxidation/fermentation-polymyxin-bacitracin-lactose (OFPBL) media (BD Biosciences) to select single B ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were pelleted and washed twice with staining buffer and suspend 1 ml of staining buffer with DAPI for an end concentration of 1 x 106 cells/ml and DAPI (0.2 ug/ml; BD Biosciences, 564907) added ...
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bioRxiv - Immunology 2024Quote: ... with phorbol 12-myristate 13-acetate (PMA, 20 ng/ml) plus ionomycin (1 mg/ml) in the presence of GolgiPlug/GolgiStop (BD Biosciences). Fluorescence was quantified using an LSR-II or Fortessa X20 flow cytometer (BD Biosciences).
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bioRxiv - Developmental Biology 2024Quote: All cell culture of all species was maintained at 37°C with 5% CO2 and 5% O2 on Matrigel (1:100, BD Corning) coated plates unless otherwise specified ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed once with FACS buffer and fixed with 1% paraformaldehyde for 30 minutes prior to analysis on an LSRII flow cytometer (BD Biosciences).
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bioRxiv - Immunology 2024Quote: ... diluted 1:10 in DI H2O for 10 minutes within 1 hour of sample collection and then washed twice with Cell Staining Buffer (BD Biosciences). For cell surface staining ...
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bioRxiv - Immunology 2023Quote: ... cells were counted then stained with optimal concentrations of the following fluorophore-antibody conjugations: BB700 anti-IgG1 (clone A85-1, BD Biosciences), BB700 anti-IgG2a (clone R2-40 ...
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bioRxiv - Microbiology 2024Quote: ... Cells were stained with rabbit anti-GRA7 (1:5000) (gift from John Boothroyd, Stanford) and rat anti-LAMP1 (1D4B, BD Biosciences) (1:500 ...