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Citations for Becton, Dickinson and Company :
5601 - 5650 of 5708 citations for Herpes Simplex Virus 1 Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... cells were dispersed into a single cell suspension using 0.05 % trypsin and subsequently incubated with an anti CD144 antibody (anti-VE-Cadherin; BD Biosciences, #562242, 1/50) for 30 min on ice ...
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Regulatory T cells control type 1-driven immunopathology restraining GM-CSF-producing helper T cellsbioRxiv - Immunology 2024Quote: ... cells were restimulated ex vivo for 4-5h at 37 °C in complete RPMI medium (RPMI supplemented with 2mM L-Glutamine and 10% fetal calf serum (FCS)) with GolgiPlug (BD, 1:1,000 dilution), phorbol 12-myristate 13-acetate (50ng/ml ...
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bioRxiv - Immunology 2024Quote: ... Purified CD4+ cells were cultured in 24-well round-bottom plates (1×106 cells/well) with plate-bound anti-CD3e (2.5 μg/ml, clone 2C11, BD Biosciences, San Diego, CA), soluble anti-CD28 (2 µg/ml ...
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bioRxiv - Cell Biology 2023Quote: ABT-737-treated or SARS-CoV-2-infected cells (detached using Trypsin/EGTA) and their respective conditioned media were combined and stained with fluorescently-labelled annexin A5 (BD Biosciences, 1:2,000) and 0.2 μM TO-PRO-3 (Life Technologies ...
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bioRxiv - Immunology 2023Quote: ... pre-coated for 1 hour at 37°C with anti-CD3 antibodies (5ug/mL, clone: 145-2C11, BD Biosciences, San Diego, CA, USA) and supplemented with soluble anti-CD28 antibodies (2ug/mL clone ...
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bioRxiv - Biochemistry 2023Quote: Plasma was obtained by withdrawing blood from eight healthy donors (specified in Supplementary Table 1) in EDTA tubes (BD Vacutainer K2E; REF 367525). The tubes were inverted three times and centrifuged at 2000xg for 20 min at 4°C ...
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bioRxiv - Physiology 2023Quote: ... p = 0.2 (sum of shared targets between 1/0 and 5/0 R-βHB ex vivo groups and BH-BD targets, divided by n), and q = 0.8 ...
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bioRxiv - Pathology 2023Quote: ... A final resuspension of splenocyte pellet with 100 µl of PBS-FBS 1% was performed before transfer to Micronic tubes (Cat# 2517810, Dutscher, France) for flow cytometry analysis (LSRFortessa™, BD Biosciences, USA). Data was analyzed using FlowJo software (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... blood cells were stained in 50 µl 2% FCS RPMI medium with antibodies against CD11C (APC, clone HC3, BD biosciences, dilution 1/100), CD11b (PercPCy5.5 or Pacific Blue ...
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bioRxiv - Microbiology 2023Quote: ... and allowed to gel over a 1-mL layer of acellular collagen in six-well culture inserts with 3-μm-pore polycarbonate filters (BD Biosciences NJ, USA). Gels were allowed to contract for 4–5 days before seeding with 3×105 HaCaT keratinocytes in serum and mitogen-supplemented DMEM/F12 raft medium ...
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bioRxiv - Developmental Biology 2023Quote: ... FGF10 expression was induced by the addition of isopropyl-1-thio-B-D-galactopyranoside to RosettaTM2(DE3)pLysS carrying pET21d-FGF10 in 2x YT medium (BD Biosciences, Cat#244020) with Carbencillin (50 µg/mL ...
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bioRxiv - Immunology 2023Quote: ... cells were washed in perm buffer (PBS, 0.5% BSA, 0.1% Saponin) and stained for IFN-γ (APC rat anti- mouse IFN-γ; 1:100; BD Biosciences, Cat. 554413) and TNF (PE rat anti-mouse TNF alpha ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then suspended in 0.5% Tween 20 (vol/vol) plus 1% BSA/PBS and incubated for 30 minutes at room temperature with anti-BrdU FITC (BD Biosciences, Cat. #347583) at a concentration of 0.5 µg/106 cells ...
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bioRxiv - Cancer Biology 2023Quote: ... 200 μL per 1x10e6 cells) with human IgG-Fc block (1:100) followed by the incubation with streptavidin-magnetic bead particles (BD Biosciences, Ref#557812) at 50 μL per 1x10e6 cells ...
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bioRxiv - Immunology 2023Quote: ... Cells were stained with flow cytometry antibodies (Supplemental Table 9) in flow cytometry staining buffer (PBS, 1% BSA) and brilliant staining buffer (563794, BD, Franklin Lakes, NJ). Cells were analyzed using a 5-laser (16UV-16V-14B-10YG-8R ...
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bioRxiv - Physiology 2023Quote: ... The platelets were then fixed with 1% formaldehyde and the fluorescence were determined using BD LSRFortessaTM cell analyzer (BD Biosciences, Mississauga, ON, Canada). A negative control with no antibodies was included.
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bioRxiv - Pathology 2023Quote: ... The culture medium was sampled by adding 1-3 drops to microscope slides followed by Gram stain (BD BBLTM Gram Stain Kits; B12539) and imaging.
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bioRxiv - Cell Biology 2023Quote: ... aeruginosa PAO1 was diluted 100-fold into 300 mL of M9 medium (1% w/v 5X M9 Salts (BD Difco, Franklin Lakes, NJ), 1.5% w/v Bacto Casamino Acids with low iron and salt content (BD Difco) ...
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bioRxiv - Neuroscience 2023Quote: ... Homogenized tissue was washed with 3-4 ml washing buffer which was fresh PBSB 1% and then was filtered through a 70-µm cell strainer (BD Bioscience, NY, USA). Then using a long tube ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were blocked with 5% skim-milk diluted in 1X TBST and incubated with primary antibodies overnight [RB (BD Pharmingen #554136, 1:500), p53 (Pierce #MA5-12453 ...
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bioRxiv - Genomics 2024Quote: ... 2.5 x 105 cells were stained for 75 minutes at room temperature with PE Mouse anti-PDX1 Clone-658A5 (BD Biosciences; 1:10) and Alexa Fluor® 647 Mouse anti-NKX6.1 Clone R11-560 (BD Bioscience ...
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bioRxiv - Cancer Biology 2024Quote: ... female athymic nude mice were injected into the mammary fat pad with 1×106 MDA-BR cells in a 100μl mixture composed of Matrigel (50μl; BD Matrigel 10mg/ml, BD Biosciences) and PBS (50μl) ...
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bioRxiv - Microbiology 2024Quote: ... and the cell pellet was stained with PBS containing 1:1000 diluted BD Fixable Viability Stain 780 (Cat. # 565388, BD Biosciences, Heidelberg, Germany) for 15 minutes at RT ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then washed at least three times with 1×PBS (pH 7.4) before flow cytometric analysis using BD Accuri C6 (BD Biosciences, San Jose, USA) with FITC channel.
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bioRxiv - Microbiology 2024Quote: Water samples prepared as previously described [35] and sediment (1 g) were enriched in 10 ml of buffered peptone water (BPW; BD DifcoTM, Becton Dickinson) or E ...
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bioRxiv - Neuroscience 2024Quote: ... The cover slips were then permeabilized with 0.3% Triton X-100 and sequentially stained with rat anti-CD44 antibody (BD Biosciences, 550538; 1:200), and corresponding Alexa 488-conjugated secondary antibodies (Invitrogen ...
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bioRxiv - Developmental Biology 2024Quote: ... slides were processed as described previously for section immunofluorescent staining for detection of mouse IgG2a anti-E-cadherin (1:500, BD Biosciences, Cat# 610181) and imaged on a Leica SP5 upright confocal ...
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bioRxiv - Immunology 2021Quote: Treatments comprising 15μl of EVs suspension in PBS corresponding to EV secretions from ~5.0 × 105 cells or PBS alone controls were injected intra-articularly 1 day post arthritis induction with 0.5ml monoject (29G) insulin syringes (BD Micro-Fine, Franklyn Lakes, USA) through the patellar ligament into the right knee joint ...
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bioRxiv - Neuroscience 2021Quote: ... Stimulated cells were stained with fluorochrome-conjugated anti-MCP-1 and anti-IL-6 Abs using the Cytofix/Cytoperm kit (BD Biosciences, San Jose, CA) to determine MCP-1 and IL-6 production ...
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bioRxiv - Microbiology 2019Quote: ... ESAT6 peptide (1 μg/ml) or TB10 peptide (1 μg/ml) for 24 hours at 37°C before the addition of GolgiPlug (BD Pharmingen, San Diego, CA). The concentration of protein and peptides used were according to previous reports [19 ...
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bioRxiv - Microbiology 2021Quote: ... and then labeled overnight at 4°C with the APC-conjugated rat anti-mouse CD45 antibody (1:100 dilution, BD Pharmingen, cat No. 559864). Cell nucleus was stained with DAPI ...
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bioRxiv - Neuroscience 2021Quote: ... was sprayed on the plantar surface of the hind right paw using a syringe of 1 ml (Tuberculin slip tip, BD, Franklin Lake, NJ, USA). Paw nociceptive responses ...
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bioRxiv - Systems Biology 2020Quote: ... HL60 and U937 cells (1 mL) were immediately fixed by addition of 0.2 mL of x5 Lyse/Fix buffer (BD Biosciences, San Jose, CA, USA) for 10 min at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... Cochlear whole mounts were immunostained overnight at 4°C with primary antibody against CTBP2 (mouse anti-CTBP2 at 1:200; BD Transduction Labs, Heidelberg, Germany). Secondary antibody (donkey anti mouse conjugated with Cy3 ...
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bioRxiv - Microbiology 2021Quote: Detection of extracellular nectin-1 in infected cells was performed using a BD LSR II or BD LSR Fortessa cytometer (BD Biosciences, San Jose, CA). Infected cells were harvested by 5mM EDTA supplemented with Dulbecco’s PBS (D-PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were incubated overnight at 4°C in solution containing 1% BSA and primary antibodies: mouse F4/80 (T45-2342, BD Biosciences, New Jersey, USA) 1:200 ...
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bioRxiv - Systems Biology 2021Quote: ... xanthus strains were grown overnight at 32°C with vigorous shaking in CTTYE broth (1% Casein Peptone [Remel], 0.5% Bacto Yeast Extract [BD Biosciences], 10mM Tris (pH 8.0), 1mM KH(H2)PO4 ...
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bioRxiv - Microbiology 2021Quote: ... were anesthetized and one 1-cm long catheter segment inserted into the peritoneum using an 18-gauge BD Spinal needle/stylette (Becton, Dickinson and Company, USA). Four hrs post-catheter implantation ...
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bioRxiv - Cancer Biology 2020Quote: Cells were treated as indicated and stained with fluorescent conjugates of annexin-V and PI (1 µg/ml final concentration) and analyzed on a FACSCanto machine (BD, Franklin Lakes, NJ, USA). Annexin V was prepared as previously described (Brumatti et al. ...
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bioRxiv - Biophysics 2022Quote: ... Cell suspensions were homogenized by passing 10 times through a precooled 1-ml syringe with a 27-gauge stainless steel needle (BD Biosciences, Franklin Lakes, NJ). The homogenate was adjusted to about 40% sucrose by the addition of 700μl of 60% sucrose prepared in TNE and placed at the bottom of an ultracentrifuge tube ...
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bioRxiv - Cancer Biology 2022Quote: qNSCs and aNSCs from the SVZ were FACS purified from GFAP::GFP mice as previously described (Codega et al., 2014) using the following antibodies: anti-mCD24 PE (1:2000; BD Pharmingen 12-0242-82), biotin conjugated anti-mCD133 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed five times and developed with tetramethylbenzidine (1-Step Ultra TMB-ELISA Substrate Solution, ThermoFisher, for RBD-ELISA, and BD OptEIA Substrate Solution, BD Biosciences, for Spike ELISA) for 5 min ...
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bioRxiv - Neuroscience 2020Quote: ... The transfer of the retinal suspension onto panning plates bound with monoclonal mouse anti-SSEA-1 (CD15)(MC480 clone) (#BD560079; BD Pharmingen, San Jose, CA) and monoclonal mouse anti-HNK-1/N-CAM (CD57)(VC1.1 clone ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed five times and developed with tetramethylbenzidine (1-Step Ultra TMB-ELISA Substrate Solution, ThermoFisher, for RBD-ELISA,and BD OptEIA Substrate Solution, BD Biosciences, for Spike ELISA) for 5 min ...
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bioRxiv - Immunology 2022Quote: Total white blood cells from 1-2 mL freshly collected blood in EDTA tubes were enriched through ammonium chloride lysis (BD Bioscience, catalog no. 555899), followed by centrifugation (300x g for 10min) ...
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bioRxiv - Cell Biology 2019Quote: ... The following day they were probed with antibodies to FLAP (Novus, IMG 3160, 1:100) and to 5-LO (BD Biosciences, 610695, RRID: AB_398018). Secondary antibodies were applied at 3 µg/mL for 1 h ...
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bioRxiv - Physiology 2021Quote: ... Sections were then washed with PBS 3 times for 15 min and incubated with PBS containing Hoechst (1:5000, #561908, BD Pharmingen, Lincoln Park, NJ) for 5 min before a final series of washes with PBS.
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bioRxiv - Microbiology 2021Quote: ... coli BL21 (DE3) strain harboring pCOLD-1-EhPTEN1 was grown at 37°C in 50 ml of LB medium (BD Difco, New Jersey, USA) in the presence of 100 µg/ml ampicillin ...
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bioRxiv - Cancer Biology 2021Quote: ... or 4T1.2 cells (1⨯106 per fat pad) in 50 μL of 50:50 (v/v) PBS:Matrigel™ (BD Biosciences, San Jose, CA, USA). Tumor growth was measured twice weekly by caliper measurements and weekly bioluminescent imaging (125 mg/kg luciferin I.P. ...
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bioRxiv - Microbiology 2019Quote: ... The mixture was filtered (Whatman #1) from which 10mL of the filtrate aliquoted into 14mL BD Falcon™ test tubes (BD Biosciences, Bedford, USA) and labeled accordingly ...