Labshake search
Citations for Lucigen :
201 - 250 of 472 citations for Cell Isolation Optimizing System since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2020Quote: ... DNA collection from cultured cells was performed using QuickExtract (Lucigen QE09050).
-
bioRxiv - Microbiology 2020Quote: ... NEB Builder reaction mixtures were transformed into E.coli EPI300 cells (Lucigen) and successful integration of the S protein cDNA was confirmed by Sanger sequencing ...
-
bioRxiv - Neuroscience 2021Quote: NeuroD1-pNOTAT was transformed into C41 competent cells (Lucigen, Middleton, WI) and induced with IPTG at 0.1mM for protein expression ...
-
bioRxiv - Microbiology 2021Quote: ... and transformed into the BAC-Optimized Replicator v2.0 Electrocompetent Cells (Lucigen). The replicon vector was maxipreped using a NucleoBond Xtra BAC kit (Takara Bio.).
-
bioRxiv - Genomics 2022Quote: ... The product was electrotransformed into Endura™ Competent Cells (Lucigen #60242) and grown on big agar plates ...
-
bioRxiv - Microbiology 2020Quote: Escherichia coli TransforMax Epi300 electrocompetent cells (Ref. EC300110, Epicentre, Madison, WI) and Saccharomyces cerevisiae VL6-48N strain [30] were used to propagate the pCC1BAC-His3 containing viral cDNA ...
-
bioRxiv - Biochemistry 2019Quote: ... The cells were lysed with 4000U of RNase-free lysozyme (Lucigen) at 37 °C for 2 hours ...
-
bioRxiv - Cell Biology 2021Quote: ... coli cells (Strand et al., 2014; Lucigen, Middleton, WI, Catalog #EC300110) were transformed via heat shock with sequence-verified pPtPBR1 episomes ...
-
bioRxiv - Physiology 2022Quote: ... sorted (eGFP+) cells was extracted using QuickExtract DNA Extraction Solution (Lucigen). EnGen Mutation Detection Kit (New England BioLabs ...
-
bioRxiv - Cell Biology 2022Quote: Ligation products were transformed into Lucigen Endura Electrocompetent Cells (Lucigen, 60242) using a Gene Pulser II (Bio-Rad Laboratories ...
-
bioRxiv - Microbiology 2023Quote: ... cells were lysed in a Ready-Lyse lysozyme solution (Epicentre Technologies) according to manufacturer’s instructions and lysates were homogenized through QiaShredder columns (Qiagen ...
-
bioRxiv - Biochemistry 2023Quote: ... The construct was transformed into chemically competent C41 (DE3) cells (Lucigen) and plated after heat shock and recovery on LB agar supplemented with ampicillin ...
-
bioRxiv - Genetics 2023Quote: ... Cell pellets were resuspended in 50 µL of Quick Extract (Lucigen), and genomic DNA was prepared per the manufacturer’s instructions.
-
bioRxiv - Genetics 2022Quote: ... The ligation products were transferred into Ecloni 10GF’ electrocompetent cells (Lucigen) using Gene Pulser Xcell Electroporation Systems (Bio-Rad) ...
-
bioRxiv - Cancer Biology 2023Quote: ... Ligated pUSEPR plasmid DNA was electroporated into Endura electrocompetent cells (Lucigen), recovered for one hour at 37⁰C ...
-
bioRxiv - Microbiology 2024Quote: ... the phagemids were electroporated into phage-competent TG1 bacterial cells (Lucigen) with the MicroPulsuerTM system (Bio-Rad) ...
-
bioRxiv - Cell Biology 2020Quote: ... ruptured cells were treated with 1 U μl−1 OmniCleave endonuclease (Lucigen) for 15 min at 37°C to release the DNA-bound protein pool ...
-
bioRxiv - Biochemistry 2021Quote: ... Cloning was performed by Gibson assembly (46) using E.cloni 10G cells (Lucigen) and point mutations were performed using the Q5 site-directed mutagenesis kit (NEB) ...
-
bioRxiv - Cancer Biology 2022Quote: DNA was extracted from cells using the QuickExtract DNA Extraction Solution (Lucigen) and sequencing was performed with 110x coverage using 100 base pair paired end read lengths ...
-
bioRxiv - Immunology 2022Quote: ... The Gibson assembly reaction was electroporated using Endura(tm) ElectroCompetent Cells (Lucigen), plated on LB + Ampicillin plates at an estimated 150,000 colonies per replicate library to limit library complexity ...
-
bioRxiv - Genomics 2019Quote: gDNA was extracted by cell lysis using QuickExtract DNA Extraction Solution (Lucigen). From a confluent culture in 96-well plate ...
-
bioRxiv - Genomics 2019Quote: RNA from 100,000 K562 cells was extracted with RNA QuickExtract (Lucigen QER090150). RNA was treated with DNaseI from the same kit ...
-
bioRxiv - Immunology 2019Quote: The recombinant plasmids were transformed into ClearColi-BL21 (DE3) electrocompetent cells (Lucigen) by heat-shock transformation ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Plasmids were grown in E.Cloni 10G Chemically Competent Cells (Lucigen, 60107-1) and were verified by Sanger sequencing (Eton biosciences) ...
-
bioRxiv - Developmental Biology 2021Quote: ... approximately 30,000 cells were lysed in 25ul Quick Extract Buffer (Lucigen #QE09050), according to the manufacturer’s directions ...
-
bioRxiv - Microbiology 2020Quote: ... BAC plasmid was delivered into BAC-Optimized Replicator v2.0 Electrocompetent Cells (Lucigen) by electroporation and bacteria was propagated according to the manufacturer’s guide ...
-
bioRxiv - Genomics 2021Quote: ... and 5 electroporations were performed into Endura electrocompetent cells (Lucigen, Middleton WI) and plated onto 24.5 cm2 LB-agar plates ...
-
bioRxiv - Microbiology 2022Quote: ... The library was electroporated into Endura electrocompetent cells (# 60242; Lucigen Bioresearch Technologies) and plasmid was prepared from > 80 million bacterial colonies (>1,000X representation) ...
-
bioRxiv - Immunology 2023Quote: 0.1 × 106 cells were collected and lysed in QuickExtract™ (Lucigen Epicentre) according to the supplier’s instructions ...
-
bioRxiv - Immunology 2023Quote: 0.1 × 106 cells were collected and lysed in QuickExtract™ (Lucigen Epicentre) according to the supplier’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... Library (50ng) was electroporated into 25µL Endura electrocompetent cells (60242-2, Lucigen). Cells from eight electroporations were pooled and rescued in 8mL of rescue media for 1hr at 37 ...
-
bioRxiv - Cell Biology 2023Quote: ... Ruptured cells were treated with 1 U/μl OmniCleave endonuclease (Lucigen, OC7850K) for 5 min at 37°C to release the DNA-bound protein pool ...
-
bioRxiv - Microbiology 2023Quote: ... Aliquots of cells were mixed with EZ-Tn5TM
transposome (Lucigen) and incubated on ice for 30 min ... -
Measuring carbohydrate recognition profile of lectins on live cells using liquid glycan array (LiGA)bioRxiv - Biochemistry 2023Quote: ... The resulting ligated DNA was transformed into electrocompetent cells E.coli SS320 (Lucigen) and propagated overnight.
-
bioRxiv - Neuroscience 2023Quote: DNA was extracted from 96-well plates of cells using QuickExtract (Epicentre) by incubation at 65°C for 6 minutes ...
-
bioRxiv - Cancer Biology 2024Quote: ... DNA was harvested from cell pellets using QuickExtract DNA Extraction Solution (Lucigen). The ROS1 kinase and C-terminal domains were PCR amplified using the primers ROS1 5707F (5’-GACAAAGAGTTGGCTGAGCTG-3’ ...
-
bioRxiv - Genetics 2023Quote: ... Final assembled products were delivered to Endura Electrocompetent Cells (Lucigen, 60242-1) via electrotransformation (Bio-Rad Gene Pulser II) ...
-
bioRxiv - Genomics 2022Quote: ... the ScriptSeq™ Index PCR Primers (Sets 1 to 4) and the FailSafe™ PCR enzyme system (all sourced from Epicentre®/Illumina® Inc., Madison, WI, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: ... the genomic DNA of edited cells were extracted using Quick Extraction kit (Lucigen). Target sites carrying gene-edited sequences were PCR amplified using gene-specific primers (Additional file 2 ...
-
bioRxiv - Molecular Biology 2019Quote: Genomic DNA was extracted from the cells using QuickExtract DNA Extraction Solution (Epicentre) or PureLink Solution (Invitrogen ...
-
bioRxiv - Molecular Biology 2019Quote: Genomic DNA was extracted from the cells using QuickExtract DNA Extraction Solution (Epicentre) following the protocol of the manufacturer and the target sites were PCR amplified using the primers listed in the supplement ordered from MilliporeSigma ...
-
bioRxiv - Microbiology 2019Quote: ... amplified and transformed into Hi-Control 10G cells according to manufactures protocol (Lucigen, Expresso™ T7 cloning and expression system) ...
-
bioRxiv - Genomics 2019Quote: ... The ligated DNA sample was used to transform electrocompetent E.coli cells (EPI300, Epicentre), using 1 µl of ligated DNA solution per transformation ...
-
bioRxiv - Neuroscience 2020Quote: ... Cells were transferred to tubes containing 3uL of lysis buffer (Epicentre, MessageBOOSTER kit), and stored at - 80° ...
-
bioRxiv - Bioengineering 2021Quote: Genomic DNA was extracted from 100,000 cells per condition using DNA QuickExtract (Lucigen), and incubated at 65°C for 15 min ...
-
bioRxiv - Genomics 2021Quote: ... and used to electroporate 1-4 tubes of Endura DUO electrocompetent cells (Lucigen) at 1.8 kV distributed over 2 cuvettes (0.1 cm gap width ...
-
bioRxiv - Cancer Biology 2021Quote: DNA was extracted from cells using Quick Extract (Lucigen, Wisconsin, USA, Cat #QE09050) and amplified using primer pairs listed in Table S4 designed to amplify 66-80 base pair segments containing the predicted cut site for each of our gRNAs listed in Table of gRNAs ...
-
bioRxiv - Cancer Biology 2021Quote: ... cells were expanded and genomic DNA extracted (QuickExtract DNA Extraction Solution; Epicentre, #QE09050), as described above ...
-
bioRxiv - Biophysics 2022Quote: ... DNA from these cells was extracted using QuickExtract DNA extraction solution (Lucigen # QE09050) and used as a template for confirmation of homologous recombination by PCR and Sanger sequencing.
-
bioRxiv - Bioengineering 2022Quote: DNA was extracted from harvested cells using the QuickExtract DNA Extraction Solution (Lucigen) according to the manufacturer’s instructions ...