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Citations for Lucigen :
1 - 50 of 472 citations for Cell Isolation Optimizing System since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: The DNA isolation procedure and WGS procedure was performed as follows: DNA was extracted using the MasterPure DNA isolation kit (Lucigen) or MasterPure Gram Positive DNA purification kit (Lucigen) ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by chromosomal DNA isolation (Quick Extract /Lucigen), PCR amplification (Sup Table 8 ...
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bioRxiv - Microbiology 2021Quote: DNA isolation from inactivated cells was carried out using MasterPure™ Gram Positive DNA Purification Kit (Lucigen, Middleton, WI, USA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: Total DNA was isolated using Masterpure Complete DNA Isolation kit (Epicentre). PCR to confirm presence of transposon was preformed using GoTaq® Green Master Mix (Promega ...
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bioRxiv - Immunology 2021Quote: ... and additionally purified with a mRNA-ONLY Eukaryotic mRNA Isolation Kit (Epicentre). Transcriptome libraries were then constructed using SOLiD total RNA-seq kit (Applied Biosystems ...
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bioRxiv - Immunology 2022Quote: ... and additionally purified with a mRNA-ONLY™ Eukaryotic mRNA Isolation Kit (Epicentre). Transcriptome libraries were then constructed using SOLiD total RNA-seq kit (Applied Biosystems ...
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bioRxiv - Genomics 2021Quote: ... and DNA extraction was carried out with MasterPure Complete DNA Isolation kit (Lucigen) as indicated by the manufacturer ...
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bioRxiv - Cell Biology 2024Quote: ... Genomic DNA was isolated from clones using QuickExtractTM DNA isolation solution (Lucigen #QE09050). PCRs were performed with KOD hot-start polymerase (Merck #71086) ...
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bioRxiv - Genetics 2022Quote: ... RNA isolation was performed according to the MasterPure™ Yeast RNA Purification Kit (Epicentre, Lucigen) manufacturer’s protocol ...
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bioRxiv - Genetics 2022Quote: ... RNA isolation was performed according to the MasterPure™ Yeast RNA Purification Kit (Epicentre, Lucigen) manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... mRNAs were purified after removal of rRNA (mRNA-ONLY™ Eukaryotic mRNA Isolation Kit, Epicentre Biotech., WI). Fluorescently labeled cRNAs derived from these transcripts were used to probe Agilent Mouse V4.0 LncRNA Array containing probes for 22,692 mRNAs (ArrayStar ...
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bioRxiv - Bioengineering 2024Quote: ... gDNA isolation was performed using the Epicentre MasterPure Gram Positive DNA Purification Kit (Lucigen, Middleton, WI, USA) according to manufacturer’s instructions and total gDNA was quantified using a nano-drop (Thermo-Fisher Scientific Waltham ...
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bioRxiv - Molecular Biology 2023Quote: ... mRNA was purified from total RNA after removal of rRNA (mRNA-ONLY™ Eukaryotic mRNA Isolation Kit, Epicentre). Then ...
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bioRxiv - Synthetic Biology 2022Quote: For isolation of genomic DNA (gDNA) of phages Master-Pure™ Complete DNA and RNA Purification Kit (Lucigen, Wisconsin, USA) was used according to the supplier’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... One of the plates was used for quick genomic DNA isolation for each of the colonies using QuickExtract DNA extraction solution (Lucigen # QE09050). 5μl of this lysate was used to set up a quick genomic PCR screening for IL-1β knockout clones using checking primers ...
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bioRxiv - Genetics 2020Quote: The DNA isolation was performed on the swim up purified sperm using the MasterPure DNA purification kit (Epicentre Biotechnologies, Madison, WI, USA) as previously described [13] ...
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bioRxiv - Bioengineering 2022Quote: Samples collected at 24 h were processed for DNA isolation using the Epicentre MasterPure Gram Positive DNA Purification Kit (Lucigen, Middleton, WI, USA). Briefly ...
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bioRxiv - Genomics 2020Quote: ... or homogenized tissues were digested with proteinase K prior to DNA isolation using MasterPure™ DNA Purification Kit (Epicentre, by Illumina, Madison, United States) according to manufacturer’s instructions.
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bioRxiv - Plant Biology 2020Quote: ... Isolation of genomic DNA from leaf powder was performed using the MasterPure™ Complete DNA and RNA Purification Kit from Epicentre (Epicentre, Chicago, USA) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... coli cells (Endura electrocompetent cells, Lucigen) following the manufacturer’s instructions transforming 90 ng of library DNA into 25 μL of electrocompetent cells for each electroporation reaction for a total of 6 reactions and the cells were pooled and plated on 6 x LB agar plates (245-mm square bioassay dish ...
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bioRxiv - Molecular Biology 2020Quote: ... The pure transcripts were capped using the ScriptCap™ m7G Capping System (Epicentre). Radiolabelled transcripts were obtained by substituting the GTP from the kit with [α32P]GTP.
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bioRxiv - Biochemistry 2022Quote: ... coli cells (Lucigen) and purified as described by Urbanek et al ...
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bioRxiv - Biochemistry 2019Quote: ... coli cells (Lucigen) by electroporation ...
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bioRxiv - Biochemistry 2019Quote: ... coli cells (Lucigen) were transformed by electroporation ...
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bioRxiv - Biochemistry 2019Quote: ... coli cells (Lucigen) were transformed with the resulting plasmid by electroporation ...
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bioRxiv - Biochemistry 2019Quote: ... coli cells (Lucigen) by electroporation ...
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bioRxiv - Biochemistry 2019Quote: ... coli cells (Lucigen) by electroporation ...
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bioRxiv - Biochemistry 2021Quote: ... coli cells (Lucigen). Cells cultured in SOC media and recovered for 45 minutes at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... TG1 cells (Lucigen) were transformed with this library by electroporation.
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bioRxiv - Synthetic Biology 2021Quote: ... coli cells (Lucigen) and then cultivated at 37°C in LB medium supplemented with kanamycin and streptomycin ...
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bioRxiv - Systems Biology 2020Quote: ... coli cells (Lucigen) using a GenePulserXCell (Biorad ...
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bioRxiv - Biochemistry 2022Quote: ... coli cells (Lucigen) were transformed with expression plasmid and a single colony was used to start an overnight culture in LB media ...
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bioRxiv - Genomics 2022Quote: ... coli cells (Lucigen) using a MicroPulser Electroporator (Bio-Rad) ...
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bioRxiv - Biochemistry 2022Quote: ... coli cells (Lucigen). To allow for fluorescent labeling of Wsp1p-PRD ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli cells (Lucigen), which were plated on LB agar supplemented with 100 µg mL−1 ampicillin ...
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bioRxiv - Biochemistry 2020Quote: ... coli cells (Lucigen) harboring the pTA-MOB conjugation helper plasmid (Strand et al. ...
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bioRxiv - Genomics 2022Quote: ... coli cells (Lucigen) using a MicroPulser Electroporator (Bio-Rad) ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli cells (Lucigen). Integration ...
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bioRxiv - Systems Biology 2023Quote: ... coli cells (Lucigen), and recovered for one hour at 37°C in Recovery Media (Lucigen) ...
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bioRxiv - Microbiology 2023Quote: ... each DNA sample was amplified in triplicate using the FailSafe™ PCR System (Epicentre, WI) and the 515FB-806RB primer pair to generate a 400 bp amplicon from the V4 variable regions of the 16S rRNA gene ...
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bioRxiv - Molecular Biology 2023Quote: ... we utilized the ScriptCap m7G Capping System and ScriptCap 2’-O-methyltransferase kit (Epicentre Biotechnologies) with [α-32P] GTP for our experiments ...
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bioRxiv - Microbiology 2019Quote: ... cloni electrocompetent cells (Lucigen) according to manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2020Quote: ... cloni® cells (Lucigen) and plated on 37 large agar plates with Kanamycin antibiotics in order to conserve library complexity ...
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bioRxiv - Genetics 2021Quote: Endura electrocompetent cells (Lucigen) were electroporated with the genome-scale CRISPR KO (Brunello ...
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bioRxiv - Bioengineering 2021Quote: ... coli electrocompetent cells (Lucigen) then plated on a 245 × 245 mm LB and kan agar plate ...
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bioRxiv - Biochemistry 2022Quote: ... coli competent cells (Lucigen). A single colony of transformed cells was grown in 3 mL lysogeny broth (LB ...
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bioRxiv - Molecular Biology 2020Quote: C41(DE3) cells (Lucigen) were transformed with the expression plasmid using standard techniques ...
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bioRxiv - Biochemistry 2022Quote: ... cloni 10G cells (Lucigen) by electroporation (1.8 kV) ...
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bioRxiv - Bioengineering 2022Quote: ... coli SS320 cells (Lucigen) containing the circular phagemid comprising the target scaffold sequence and a M13cp helper plasmid ...
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bioRxiv - Genetics 2019Quote: ... Copy cutter cells (Epicentre) were used for propagation as the slc12a7a/kcc4a gene product was toxic to commonly used bacteria when at high copy number ...