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Citations for Lucigen :
451 - 472 of 472 citations for Cell Isolation Optimizing System since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... at 37°C (1 – 2 h) and 1 μl from PCR reaction aliquots were transformed in electrocompetent E.coli Cloni® 10G cells (Lucigen, USA). Colonies were screened by colony PCR and sequenced.
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bioRxiv - Microbiology 2021Quote: ... OmRV-fragment2/pMA (ampicillin resistant) and pACYC177 low-copy-number plasmids were transformed into 10G chemically competent cells (Lucigen, E. cloni), respectively ...
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bioRxiv - Molecular Biology 2021Quote: DNA was isolated from HTT Q71-GFP-expressing HEK293 cells through MasterPure™ Complete DNA and RNA Purification Kit (Epicentre®) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was removed and cells were resuspended in 7 mL PBS-RI (1x PBS supplemented with 0.1 U/µL NxGen RNase inhibitor (Lucigen, CN 30281)) ...
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bioRxiv - Molecular Biology 2020Quote: ... pombe DNA was extracted from cells grown to log phase at 32°C using MasterPureTM Yeast DNA purification kit (Lucigen/Epicentre). Genomic DNA of Ch16-Tel-DSB ...
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bioRxiv - Cell Biology 2023Quote: ... Genomic DNA from CD34+ cells or Bone Marrow of transplanted mice was extracted with the QuickExtract™ DNA Extraction Solution (Lucigen). For CD33 and each off-target site ...
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bioRxiv - Plant Biology 2023Quote: ... Thirty thousand cells of each clone were added to 100 μL of Quick Extract™ DNA Extraction Solution (Lucigen, Wisconsin, USA). DNA was extracted by heat treatment (65°C for 6 min and 98°C for 2 min) ...
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bioRxiv - Systems Biology 2023Quote: ... Cells were counted and 1e6 cells for each sample were collected and washed with PBS before RNA were extracted by QuickExtract RNA Extraction Solution (Lucigen, QER090150). SuperScript IV VILO Master Mix (Thermo Scientific ...
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bioRxiv - Genetics 2023Quote: Genomic DNA was extracted from 2.5×105 Neuro2A cells with 250 μL of QuickExtract DNA Extraction Solution (Lucigen, Middleton, WI, USA) or from 30 mg of mouse liver using a EZ-10 Spin Column Animal Genomic DNA Miniprep Kit (Bio Basic ...
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bioRxiv - Cancer Biology 2024Quote: Genomic DNA for CRISPR/Cas9 genotyping was isolated from cell lines and organoids by using Quick Extract DNA Extraction Solution (Lucigen, #QE09050). For organoids ...
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bioRxiv - Molecular Biology 2021Quote: ... whole genome sequencing was performed on DNA isolated from approximately 30 OD600 yeast cells using Lucigen MasterPure Yeast DNA Purification kit (Lucigen catalog: MMPY80200) according to the manufacturer’s directions ...
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bioRxiv - Microbiology 2020Quote: ... and a mix of 600 µl of tissue and cell lysis solution and 2 µl Proteinase K from the MasterPure Complete DNA and RNA Purification Kit (Epicentre-Lucigen, Middleton, WI) was added to each sample tube ...
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bioRxiv - Cancer Biology 2021Quote: ... the ligation products were purified by removing the enzymes and transformed into 500 μl of electroporation competent TG1 cells (Lucigen, Middleton, WI) to make the library.
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bioRxiv - Synthetic Biology 2022Quote: ... 2 uL of eluent was then electroporated into each of 2 tubes of 50 uL Endura electrocompetent cells (Lucigen, Cat#60242-2) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... and a mix of 600 µl of tissue and cell lysis solution and 2 µl Proteinase K from the MasterPure Complete DNA and RNA Purification Kit (Epicentre-Lucigen, Middleton, WI) was added to each sample tube ...
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bioRxiv - Genomics 2021Quote: ... Single cells were clonally expanded and genomic DNA was extracted from half the clonal population using QuickExtract™DNA Extraction Solution (Epicentre cat # QE09050) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... After the wash step cells were incubated in 200 ul PBS with 350u/ul of lysozyme solution (Epicentre ready-lyse, Epicentre Madison WI USA) for 30 minutes at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA extraction was carried out by lysing the microbes in fresh frozen tissue samples using Yeast Cell Lysis Buffer (Epicentre, Madison, WI, USA) and bead beating ...
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bioRxiv - Neuroscience 2019Quote: ... Genomic DNA from cell lines growing on 96-well plates was extracted by 25 µl QuickExtract DNA extraction solution (Epicentre #QE09050, Madison, WI, USA), further diluted to 200 µl with H2O ...
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bioRxiv - Microbiology 2021Quote: ... After the wash step cells were incubated in 200 ul PBS with 350u/ul of lysozyme solution (Epicentre ready-lyse, Epicentre Madison WI USA) for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Swabs were incubated for one hour at 37°C with shaking in 300μL yeast cell lysis solution (from Lucigen MasterPure Yeast DNA Purification kit) and 10,000 units of ReadyLyse Lysozyme solution (Lucigen) ...
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bioRxiv - Genomics 2023Quote: ... DNA was isolated by using the instructions for cell samples of the MasterPure™ Complete DNA and RNA purification kit (Epicentre, Madison, WI, USA).