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Citations for Lucigen :
251 - 300 of 412 citations for Probable U3 small nucleolar RNA associated protein 11 UTP11 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Lysates containing 3 μg of total RNA were treated with 20 U of RNase I (Lucigen) for 45[min at 25°C and then subjected to a sucrose cushion ultracentrifugation at 100,000[rpm for 1[h at 4°C with Optima MAX-TL ultracentrifuge and TLA-110 rotor (Beckman Coulter) ...
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bioRxiv - Genomics 2024Quote: ... Monophosphorylated RNAs were selectively degraded by 1 hour incubation with Terminator 5’-Phosphate-Dependent Exonuclease (Lucigen). Subsequently ...
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bioRxiv - Molecular Biology 2024Quote: ... 40-60 μg of RNA from the lysate were treated with RNAse I (Lucigen, Cat. #N6901K) for 50 minutes at room temperature while rotating ...
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bioRxiv - Microbiology 2024Quote: ... DNA was extracted using the Epicentre MasterPure Complete DNA and RNA Purification Kit (Lucigen, Middleton WI) following the DNA purification protocol for cell samples and quantified using the Qubit dsDNA high-sensitivity assay protocol and a Qubit 3.0 fluorimeter (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... Selected clones lacking the expression of protein-of-interest were sequenced to confirm the knockout: cells were resuspended in QuickExtract (Lucigen), incubated at 65 °C for 15 min ...
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bioRxiv - Microbiology 2020Quote: ... rRNAs) was achieved by incubation of the RNA with the Terminator 5’-Phosphate-Dependent Exonuclease (TEX, Lucigen). For this purpose ...
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bioRxiv - Developmental Biology 2021Quote: Riboprobes and double-stranded RNA (dsRNA) for src-1 were generated by in vitro transcription (NxGen, Lucigen) as described previously (Petersen and Reddien ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 μg of rRNA-depleted RNA were treated with 1 U Terminator 5’-Phosphate-Dependent Exonuclease (Epicentre) in the 1x Buffer A in the presence of 40 U RNaseOUT in a 50 μL reaction at 30 °C for 1 h ...
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bioRxiv - Synthetic Biology 2020Quote: ... 400ng of total RNA was used to produce cDNA using MMLV High Performance Reverse Transcriptase (Lucigen RT80125K). TaqMan qPCR was performed using PrimeTime® Gene Expression Master Mix IDT ...
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bioRxiv - Genetics 2020Quote: ... and ribosomal RNA was depleted using the Ribo Zero Gold Magnetic system (Epicentre/Illumina, San Diego, CA). RNA-seq libraries were prepared with the ScriptSeq v2 kit (Epicentre ...
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bioRxiv - Biochemistry 2021Quote: ... RNAs were hydrolyzed by addition of 250mM NaOH and the cDNAs were circularized using CircLigase II (Lucigen). The circularized cDNAs were sent to Genomics Core at Scripps Florida for deep sequencing using either single-end or pair-end Illumina NextSeq 500 platform.
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bioRxiv - Biochemistry 2021Quote: ... Barcoded RNAs were pooled and depleted of rRNA using the RiboZero rRNA depletion kit (Epicentre, Madison, WI). These pools of barcoded RNAs were converted to Illumina cDNA libraries in three main steps ...
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bioRxiv - Microbiology 2020Quote: ... with key features being that RNA was rRNA depleted using RiboZero and libraries constructed using ScriptSeq (Epicentre). Samples were sequenced by paired-end sequencing on the HiSeq platform (Illumina) ...
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bioRxiv - Microbiology 2021Quote: ... 5’PPP structures were then converted into 5’P ends using RNA 5’ Polyphosphatase (5’PP, Epicentre), to which RNA adapters were ligated ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 µg of purified RNA was used in cDNA synthesis with MMLV HP Reverse Transcriptase (Lucigen RT80125K) and 100 ng random hexamers (IDT) ...
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bioRxiv - Molecular Biology 2022Quote: RNA was extracted from 1.5 mL of logarithmically growing cells (OD600 ~0.6-0.8) using the Masterpure Yeast RNA Purification kit (Epicentre). Residual genomic DNA was removed using the Turbo DNA-free kit (Ambion) ...
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bioRxiv - Genetics 2022Quote: ... 50ng ribo-depleted RNA was used for library preparation with the Scriptseq kit (Epicentre, cat. no. SSV21124) following the manufacturer’s protocol but with the following modifications ...
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bioRxiv - Molecular Biology 2020Quote: Only cytoplasmic RNA was treated with the Ribo-Zero Magnetic Gold Kit for human/mouse/Rat (Epicentre) to remove ribosomal RNA ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA was extracted independently for each specimen using the MasterPure Complete DNA and RNA purification Kit (Epicentre) following manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... total RNA was subjected to rRNA-depletion using RiboZeroTM rRNA Removal Kit (EpiCentre Inc., Madison, WI, USA). Double stranded cDNA synthesis was performed with rRNA-depleted mRNA using ScriptSeqTM v2 RNA-Seq Library Preparation kit (EpiCentre Inc. ...
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bioRxiv - Molecular Biology 2021Quote: ... 5ug of RNA from individual tissues were treated with 15 units of RNAse R (Epicentre, Illumina, USA) for 15 minutes at 37 degrees Celsius ...
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bioRxiv - Microbiology 2023Quote: ... for raw greywater samples and MasterPure™ Complete DNA and RNA Purification Kit (Lucigen, product code MC85200) for treated greywater samples ...
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bioRxiv - Microbiology 2023Quote: ... ribosomal RNA was removed using the Ribo-Zero rRNA removal kit for Gram-negative bacteria (Epicentre/Illumina), and libraries were prepared with the ScriptSeq Complete kit for bacteria (Epicentre/Illumina ...
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bioRxiv - Microbiology 2023Quote: ... rRNA depletion from total RNA was performed using the Ribo-Zero magnetic kit (Epicentre Biotechnologies, United States) and the mRNA was chemically fragmented to short pieces (200 nt ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was extracted by using the MasterPure complete DNA and RNA purification kit (Epicentre, Madison, WI) as described by the manufacturer ...
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bioRxiv - Molecular Biology 2022Quote: Ribosomal RNAs (rRNA) were removed using the Epicentre Ribo-zero® rRNA Removal Kit (Epicentre, Madison, WI). For the whole biopsies ...
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bioRxiv - Genomics 2022Quote: ... DNA was extracted with the MasterPure™ Complete DNA and RNA Purification Kit (Epicentre, Cat. No.: MC85200). The Qubit 3.0 fluorometer and Nano-300 (Allsheng™ ...
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bioRxiv - Molecular Biology 2022Quote: ... the lysates containing 10 µg of total RNA were treated with 2 U/µg RNase I (Lucigen) at 25°C for 45 min ...
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bioRxiv - Microbiology 2023Quote: ... genomic DNA was extracted from concentrated cells using the MasterPure complete DNA and RNA purification kit (Epicentre) and sequenced using P6 chemistry on a RS II instrument (Pacific Biosystems) ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 μg of total RNA were treated by 1MBU of DNAse (BaseLine-Zero™ DNAse, Epicentre, USA) for 20 min at 37°C to remove residual genomic DNA contamination ...
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bioRxiv - Genetics 2022Quote: ... MTR4 or mtr4-1 cells was isolated using the MasterPure™ Yeast RNA Purification Kit (Epicentre, Lucigen). Cells were incubated in 2 mL of Leu-minimal medium at 30°C and grown to saturation overnight ...
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bioRxiv - Genetics 2022Quote: ... MTR4 or mtr4-1 cells was isolated using the MasterPure™ Yeast RNA Purification Kit (Epicentre, Lucigen). Cells were incubated in 2 mL of Leu-minimal medium at 30°C and grown to saturation overnight ...
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bioRxiv - Microbiology 2022Quote: Genomic DNA from the samples was isolated using MasterPure Complete DNA & RNA Purification Kit (Lucigen, Middleton, WI) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The PCR product was purified using the Master Pure Complete DNA & RNA Purification Kit (Epicentre, Madison, USA) and Sanger sequenced by Seqlab (Microsynth Seqlab GmbH ...
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bioRxiv - Microbiology 2023Quote: ... The sample was spun down at 10,000 rcf for 1 min and the supernatant was added to 150 μl of protein precipitation reagent (Epicentre, Lucigen, Middleton, WI). Remaining steps followed the recommended PureLink Genomic DNA Mini Kit (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... The sample was spun down at 10,000 rcf for 1 min and the supernatant was added to 150 μl of protein precipitation reagent (Epicentre, Lucigen, Middleton, WI) and vortexed for 10s ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 μg of total RNA was subjected to RNase R treatment (29) with 5U RNase R (Lucigen, RNR07250) at 37°C for 30 min and then placed on ice ...
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bioRxiv - Genomics 2020Quote: We extracted high molecular weight (HMW) genomic DNA using the Masterpure Complete DNA and RNA purification kit (Lucigen), using the protocol for tissue samples ...
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bioRxiv - Bioengineering 2021Quote: ... The dsDNA library was then converted to modified-base RNA using the Durascribe T7 Transcription Kit (Lucigen, MA170E). This kit produces RNA that is resistant to RNase A degradation through the replacement of canonical CTP and UTP with 2’-fluorine-dCTP (2’-F-dCTP ...
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bioRxiv - Immunology 2021Quote: ... the GeCKOv2 plasmid library with 6 guide RNAs per human gene was propagated in Endura ElectroCompetent cells (Lucigen), and lentivirus stocks were generated by co-transfecting 293T cells with the GeCKOv2 plasmid library ...
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bioRxiv - Physiology 2021Quote: ... mRNA was purified from 100 ng of total RNA by using a Ribo-Zero rRNA removal kit (Epicentre) to deplete ribosomal RNA and convert into double-stranded complementary DNA by using an NEBNext mRNA Second Strand Synthesis Module (E6111L) ...
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bioRxiv - Plant Biology 2021Quote: ... complementary RNA (cRNA) was prepared with the AmpliCap-Max™ T7 High Yield Message Maker Kit (Epicentre Biotechnologies). Oocyte preparation and cRNA injection were performed as previously described [94] ...
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bioRxiv - Microbiology 2021Quote: ... 1000 ng of total RNA was used to remove the DNA contamination using Baseline-ZERO™ DNase (Epicentre) following the manufacturer’s instructions followed by purification using the RNA Clean & Concentrator-5 columns (Zymo Research) ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 μg RNA was used for reverse transcription with 0.5 μl MMLV HP reverse transcriptase (Lucigen cat # RT80125K) per 20 μl rxn ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA was extracted from 2ml of cells at OD595 = 0.8-1.0 using the Masterpure Yeast RNA Purification Kit (Lucigen). Small RNAs were extracted by resuspending 50ml of pelleted cells at OD595 = 0.8-1.0 in 50 mM Tris–HCl pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... two µg of total RNA were treated with 5 U of RNase R (cat. # RNR07250, Lucigen, Middleton, WI) for 40 min at 37 °C ...
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bioRxiv - Genomics 2022Quote: Equal amounts of RNA (2µg) were incubated with or without 20 U of Rnase R (RNR07250, Epicentre Biotechnologies) and 40 U of RiboLock RNase inhibitor (EO0381,Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... 1000 ng of total RNA was used to remove the DNA contamination using Baseline-ZERO™ DNase (Epicentre) following the manufacturer’s instructions followed by purification using the RNA Clean & Concentrator-5 columns (Zymo Research) ...
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bioRxiv - Molecular Biology 2020Quote: ... the (+)SHAPE and (−)SHAPE RNA samples were treated with Terminator™ 5′-Phosphate-Dependent Exonuclease (TER51020, EPICENTRE co.), which processively digests RNA with 5′-monophosphate ends ...
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bioRxiv - Microbiology 2020Quote: ... total RNA was subjected to rRNA-depletion using RiboZero™ rRNA Removal Kit (EpiCentre Inc., Madison, WI, USA). Double stranded cDNA synthesis was performed with rRNA-depleted mRNA using ScriptSeq™ v2 RNA-Seq Library Preparation guide (EpiCentre Inc. ...