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Citations for Lucigen :
1 - 50 of 412 citations for Probable U3 small nucleolar RNA associated protein 11 UTP11 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... RNA samples for small RNA-seq were treated with 5’polyphosphatase (Lucigen) and prepared using the SMARTer Apollo system with modifications for small RNA library preparation ...
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bioRxiv - Molecular Biology 2020Quote: ... The small RNA fraction was treated with 20U of RNA 5’ Polyphosphatase (TAP, Lucigen), in a 50μl reaction at 37°C for 1h ...
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bioRxiv - Plant Biology 2023Quote: ... the RNA samples were used for library construction using ScriptMiner Small RNA-seq library preparation Kit (Epicentre) and sequencing was performed on an Illumina NextSeq500.
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bioRxiv - Genetics 2020Quote: ... PCR was performed using EconoTaq and associated PCR reagents (Lucigen) with 4 μL of crude DNA lysate created as described previously 24 from ear punch biopsy specimens of 8 to 14-day-old mice ...
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bioRxiv - Developmental Biology 2023Quote: ... the 3′ ends of small RNA were ligated to an adapter using T4 truncated RNA ligase (Lucigen, Cat# LR2D11310K and NEB, Cat# M0242L), followed by the ligation of the 5′-end adaptors by T4 ligase ...
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bioRxiv - Genetics 2020Quote: ... using their associated buffers or alternatively FailSafe PCR PreMix buffers (Epicentre). Fast SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Cell Biology 2023Quote: ... we performed sucrose cushion to pellet the ribosome-associated mRNAs and proceeded with RNAse I digestion (10 U/μl by the Epicentre definition) for 30ug of RNA ...
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bioRxiv - Developmental Biology 2022Quote: ... yolk sacs were dissected and a small fraction was placed in QuickExtract DNA Extraction Solution (Lucigen, QE09050) and processed according to manufacturer’s protocols.
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A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosabioRxiv - Bioengineering 2023Quote: ... and derived keratinocytes from three patients (11 total samples) with the MasterPure Complete DNA Purification Kit (Lucigen #MC85200). The gDNA yield was quantified by the Qubit dsDNA high-sensitivity (HS ...
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bioRxiv - Cell Biology 2022Quote: ... At this stage a small aliquot of cells (75% of the plate) was lysed in 200 μL of QuickExtract DNA Extraction solution (Lucigen) and gDNA was prepared by heating to 65° C for 10 minutes followed by heat inactivation at 95° C for 2 minutes ...
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bioRxiv - Genomics 2021Quote: ... 11 μL purified CUT&Tag-DNA was used in the reaction (11 μL DNA, 2 μL 10 μM Tn5mC-ReplO1 oligo, 2 μL 10× Ampligase buffer (Lucigen), 2 μL dNTP mix (2.5mM each ...
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bioRxiv - Microbiology 2021Quote: ... eDNA was purified from the samples by removing the proteins and RNA using MasterPure Gram Positive DNA Purification Kit (Epicentre, Madison, WI, USA), and the DNA concentration was measured using NanoVue Plus (GE Healthcare ...
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bioRxiv - Systems Biology 2022Quote: ... RNA was harvested using RNA QuickExtract (Lucigen). Turbo DNAse (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... RNA extracted using MasterPure RNA Purification Kit (Epicentre), and treated with TURBO DNase as described ...
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bioRxiv - Microbiology 2022Quote: ... Protein precipitation reagent (Lucigen) was added and samples were spun at maximum speed ...
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bioRxiv - Cell Biology 2023Quote: ... 5 μg of total RNA was treated with the RNA processing enzyme RNA 5′-polyphosphatase (Epicentre) to convert 5′-triphosphate RNA or 5′-diphosphorylated RNA to 5′-monophosphate RNA without dephosphorylating monophosphorylated RNA ...
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bioRxiv - Genetics 2024Quote: ... RNA was purified using RNA Clean & Concentrator - 5 kit and subsequently treated with RNA 5′ Polyphosphatase (Lucigen) for 30 minutes at 37°C ...
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bioRxiv - Systems Biology 2021Quote: RNA was extracted using MasterPure Yeast RNA Purification Kit (Lucigen), and treated with TURBO DNase (ThermoFischer ...
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bioRxiv - Cancer Biology 2023Quote: RNA-sequencing (RNA-seq) was performed using either ScriptSeq (EpiCentre) library preparation or using the KAPA RNA HyperPrep Kit (RiboErase HMR ...
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bioRxiv - Microbiology 2024Quote: ... MPC protein precipitation reagent (Lucigen) was added ...
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bioRxiv - Microbiology 2024Quote: ... MPC protein precipitation reagent (Lucigen) was added ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA was extracted using the MasterPure Yeast RNA Purification Kit (Epicentre). cDNA was prepared using ProtoScript II Reverse Transcriptase (NEB M0368X ...
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bioRxiv - Systems Biology 2020Quote: ... Total-RNA (250ng) was depleted of ribosomal RNA using RNaseH (Epicentre) and ribosomal RNA targeting oligonucleotides based on a protocol by Adiconis et al. ...
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bioRxiv - Microbiology 2021Quote: ... RNA was extracted using the MasterPure Yeast RNA purification kit (Lucigen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... total RNA was prepared using the MasterPure RNA Purification Kit (Epicentre) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... RNA extraction was done using the ‘MasterPure Yeast RNA Purification Kit’ (Epicentre) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA extractions were performed using MasterPure™ RNA Purification Kit (Epicentre®) according to the manufacturer protocol ...
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bioRxiv - Microbiology 2020Quote: ... 7.5 μg RNA (harvested using the MasterPure Yeast RNA Purification Kit (Epicentre)) was DNAse treated with the Turbo DNA-free Kit (Invitrogen) ...
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bioRxiv - Biochemistry 2023Quote: ... Total RNA was extracted using the MasterPure Yeast RNA Purification Kit (Lucigen). First strand cDNA synthesis was performed using the EasyScript Plus cDNA Synthesis Kit (Lamda Biotech ...
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bioRxiv - Microbiology 2023Quote: Total RNA was isolated using the MasterPure Yeast RNA Purification Kit (Epicentre). cDNA was synthesized using the PrimeScript RT Master Mix (Takara).
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bioRxiv - Cell Biology 2024Quote: ... the Ribo-Zero treated RNA was used to create multiplexed RNA-seq libraries using the ScriptSeq™ v2 RNA-Seq Library Preparation Kit (Epicentre) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... Total RNA was extracted using the Masterpure Yeast RNA extraction kit (Lucigen MPY03100) as per the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... Total RNA was depleted of ribosomal RNA with the RiboZero gold kit (Epicentre), and enriched mRNA was applied to library preparation according to manufacturer’s protocol (NEBNext Ultra) ...
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bioRxiv - Genetics 2020Quote: ... Total RNA was prepared using MasterPure™ Yeast RNA Purification Kit (Epicentre, Lucigen) according to manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: ... Total RNA was prepared using MasterPure™ Yeast RNA Purification Kit (Epicentre, Lucigen) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was circularized using T4 RNA ligase 1 (Epicentre or New England BioLabs) and reverse transcribed using Superscript III (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... RNA extraction was performed using to MasterPure Yeast RNA Purification kit protocol (Epicentre) according to manufacturer instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: RNA was extracted using the MasterPure Complete DNA and RNA Purification Kit (Lucigen). Samples consisted of pools of two individuals per line resulting in 16 flies per each sex- ...
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bioRxiv - Genetics 2023Quote: Total RNA was isolated using the MasterPure™ Yeast RNA purification kit (Lucigen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and RNA was isolated with the MasterPure™ Yeast RNA Purication Kit (Epicentre MPY03100). For RNA sequencing ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were harvested and RNA extracted with MasterPure™ Yeast RNA Purification Kit (Epicentre). Samples were vacuum dried and sent to Genewiz (South Plainfield ...
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bioRxiv - Genomics 2020Quote: ... Yeast total RNA was extracted with the MasterPure Yeast RNA Purification Kit (Lucigen MPY03100) as per the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... Total RNA was depleted of ribosomal RNA with the Ribo-Zero Gold kit (Epicentre). Ribosome-depleted RNA was used to create multiplexed RNA-seq libraries (NEBNext Ultra ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA was extracted from cells using the MasterPure RNA Purification Kit from Epicentre following the manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: ... Total RNA was extracted using a MasterPure™ Yeast RNA Purification Kit (Lucigen MPY03100), the RNA concentration was determined on a Nanodrop 2000C (Thermo Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA was extracted using Epicentre MasterPure yeast RNA purification kit (Epicentre, Madison, WI) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA was extracted using Epicentre MasterPure yeast RNA purification kit (Epicentre, Madison, WI) according to manufacturer’s instructions and stored in RNase-free Tris-EDTA buffer ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and RNA was extracted using MasterPure™ RNA Purification Kit (Epicentre, Madison, WI, USA) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: T7 RNA polymerase (Lucigen NxGen)
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bioRxiv - Molecular Biology 2021Quote: ... 50U T7 RNA Polymerase (Lucigen), 1 μL RNase Inhibitor and 50~100 ng DNA template ...