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Citations for Lucigen :
101 - 150 of 412 citations for Probable U3 small nucleolar RNA associated protein 11 UTP11 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Ribosomal RNA (rRNA) was depleted from RNA used for transcriptome analysis using Ribo-Zero rRNA Removal Kit (Human/Mouse/Rat) (Epicentre) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... 50 ng of rRNA-depleted RNA was used to construct sequencing libraries using a ScriptSeq v2 RNA-seq Library Preparation Kit (SSV21124, Epicentre). Twelve PCR cycles were used for amplification of the libraries ...
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bioRxiv - Microbiology 2021Quote: ... ribosomal RNA molecules were depleted from the total RNA samples using the Ribo-Zero rRNA Removal Kit for bacteria (Epicentre), and small RNAs (< 200 nt ...
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bioRxiv - Molecular Biology 2021Quote: ... Total RNA was extracted from cell pellets as described (Greenstein et al 2018) using the Masterpure yeast RNA extraction kit (Lucigen). cDNA was produced from 2-3 g total RNA as described (Greenstein et al 2018 ...
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bioRxiv - Plant Biology 2021Quote: ... 4 μg of purified RNA was depleted of ribosomal RNA using a Ribo-Zero rRNA Removal Kit - Plant Leaf (Epicentre) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Fig 5B-D and Fig EV5 were obtained as follows: Total RNA was extracted from cells using the MasterPure Yeast RNA Purification Kit including a DNase treatment step (Epicentre), and converted to cDNA using random primers and the RevertAid Reverse Transcriptase kit (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... are listed in Table S3 and RNA extraction was carried out using Master Pure yeast RNA extraction purification kit (Epicentre) from budding cells and cells incubated with serum ...
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bioRxiv - Microbiology 2021Quote: Total RNA was extracted from harvested cultures using the Masterpure™ Complete DNA and RNA Kit (Lucigen Corporation, Middleton, Wisconsin). RNA was subsequently purified using the Zymo RNA Clean and Concentrator™ Kit (Zymo Research ...
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bioRxiv - Microbiology 2020Quote: ... rRNAs and other uncapped RNA species were depleted from RNA samples using the Terminator™ 5’-Phosphate-Dependent Exonuclease (Lucigen). Following a standard phenol-chloroform-isoamyl precipitation ...
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bioRxiv - Cell Biology 2022Quote: ... listed in Table S2 or previously described [89] and RNA extraction was carried out using Master Pure yeast RNA extraction purification kit (Epicentre). All PCR amplified products were confirmed by sequencing (Eurofins MWG Operon ...
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bioRxiv - Plant Biology 2022Quote: ... Strand-specific libraries were prepared from ribosomal RNA-depleted total RNA using ScriptSeq complete kit for plant leaf (Epicentre; BPL1224) following manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... The RNA samples were polyA-tailed and 5’-PPP-ends were trimmed to 5’-P-ends via RNA 5’-polyphosphatase (Epicentre). First-strand cDNA synthesis was performed using an oligo(dT)-adapter and M-MLV reverse transcriptase followed by high-fidelity PCR amplification of the cDNA using primers suitable for Illumina TruSeq sequencing ...
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bioRxiv - Microbiology 2022Quote: RNA from endodontic samples was extracted using the MasterPure Complete DNA and RNA Purification Kit (Epicentre Technologies, Madison, WI, USA) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was extracted from 1 × 107 cells in exponential growth phase using the Masterpure Yeast RNA Purification Kit (Epicentre) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... The RNA was then treated with polyphosphatase (Lucigen) before acid phenol chloroform extraction and RNA precipitation ...
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bioRxiv - Bioengineering 2020Quote: ... 1.5 U/µl NextGen T7 RNA polymerase (Lucigen), 1 mM of each rNTP (NEB) ...
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bioRxiv - Microbiology 2021Quote: ... samples were treated with RNA 5‘ polyphosphatase (Lucigen). The 5‘ Illumina TruSeq sequencing adapter carrying sequence tag TAATGCGC was ligated to newly formed 5‘-P RNA ends ...
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bioRxiv - Genetics 2022Quote: ... and the MasterPureTM Yeast RNA Purification Kit (Epicentre). cDNAs were synthesized with the FastQuant RT Kit (with gDNase ...
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bioRxiv - Developmental Biology 2021Quote: ... In brief, RNA was treated with 20 μl RNase R digestion reaction (2 μg RNA, 1 μl RNase R (Lucigen, #RNR07250), 2 μl 10x RNase R reaction buffer ...
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bioRxiv - Developmental Biology 2022Quote: ... 5μg of RNA was subjected to ribosomal and mitochondrial RNA depletion using the RiboZero Gold kit (Human/Mouse/Rat, Epicentre #MRZG12324) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Total RNA was isolated from yeast and contaminating DNA was depleted using the MasterPure Yeast RNA Purification Kit (Epicentre, Madison, WI) protocol with minor changes as previously described (Carrocci et al. ...
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bioRxiv - Systems Biology 2023Quote: ... Cells were counted and 1e6 cells for each sample were collected and washed with PBS before RNA were extracted by QuickExtract RNA Extraction Solution (Lucigen, QER090150). SuperScript IV VILO Master Mix (Thermo Scientific ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were removed by precipitation with MCP solution (Lucigen, WI, USA) and the supernatant was collected after centrifugation at 17,000 x g for 10 min at 40C ...
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bioRxiv - Molecular Biology 2020Quote: ... we used the MasterPure RNA purification kit from Epicentre instead of phenol/chloroform extraction to purify RNA after the dephosphorylation and ligation steps.
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bioRxiv - Genetics 2020Quote: ... using Masterpure complete DNA and RNA purification kit (Lucigen), genomic DNA for whole genome sequencing was isolated ...
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bioRxiv - Microbiology 2022Quote: ... the transcripts were dephosphorylated by RNA polyphosphatase (RPP, Lucigen) generating a 5’monophosphate ...
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bioRxiv - Genetics 2024Quote: ... samples were further treated with RNA 5′- polyphosphatase (Epicentre) as previously described[26].
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bioRxiv - Genomics 2020Quote: ... 1ug of RNA was incubated with 5U of RNaseR (Epicentre) for 45 min at 37C in a 14ul reaction volume to digest all linear RNA ...
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bioRxiv - Microbiology 2020Quote: ... Ribosomal RNA was depleted using Ribo-ZERO magnetic kit (Epicentre). The transcripts were fragmented and used as templates to generate strand-specific cDNA libraries by TruSeq Stranded Total RNA LT Sample Prep kit (Illumina) ...
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bioRxiv - Microbiology 2022Quote: ... The ScriptSeq™ v2 RNA-Seq Library Preparation Kit (Epicentre) was used to make the libraries according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: The ScriptSeq™ v2 RNA-Seq Library Preparation Kit (Epicentre) was used to prepare directional multiplex libraries ...
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bioRxiv - Molecular Biology 2021Quote: ... ScriptSeq v2 RNA-Seq library preparation kit (Epicentre Technologies, USA), TruSeq SBS Kit v3-HS (Illumina ...
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bioRxiv - Cell Biology 2022Quote: ... pombe cells using the MasterPure Yeast RNA Purification Kit (Epicentre). The procedure for removal of DNA contamination was as per the specification of the manufacturer but performed twice to efficiently remove the DNA from the samples ...
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bioRxiv - Microbiology 2022Quote: ... depleted in ribosomal RNA (Ribo-Zero rRNA Removal Kit, Epicentre) and reverse transcribed into cDNA ...
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bioRxiv - Microbiology 2024Quote: ... Ribosomal RNA (rRNA) was depleted (Ribo-ZeroTM Gold Kit, Epicentre), cDNA libraries were built with TrueSeq total RNA sample kit (Illumina ...
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bioRxiv - Cell Biology 2024Quote: ... We treated 10 μg of RNA with RNase I (Epicentre) for 45 min at 25°C ...
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bioRxiv - Neuroscience 2023Quote: ... RNA was incubated with 20 U RNase R (Lucigen RNR07250) for 30 mins at 37 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... Ribosomic RNA was depleted with Ribo-Zero™ Gold Kit (Epicentre). RNA-seq libraries were prepared with SMART-Seq® Stranded Kit (Takara-Clontech) ...
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bioRxiv - Developmental Biology 2021Quote: ... Ribosomal RNA was depleted using the Ribo-Zero magnetic kit (Epicentre). Sequencing libraries of ribosome protected fragments were generated using the ARTseq™ Ribosome Profiling Kit (Epicentre ...
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bioRxiv - Molecular Biology 2021Quote: ... Ribosomal RNA was removed using Ribo Zero Gold (Epicentre/Illumina; 20020596) using the manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: ... RNA-seq libraries were prepared with the ScriptSeq v2 kit (Epicentre) and subjected to paired-end sequencing (2 x 100 bp ...
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bioRxiv - Microbiology 2021Quote: ... Purified RNAs were then treated with TAP (Tobacco Acid Pyrophosphatase, Epicentre) to convert 5′ ends of RNA to monophosphate ...
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bioRxiv - Cell Biology 2022Quote: The MasterPure™ Complete DNA and RNA Purification kit (Epicentre, MC85200) or the RNeasy kit (Qiagen ...
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bioRxiv - Genomics 2022Quote: ... rRNA depleted islet RNA was digested using RNase R (RNR07250, Lucigen) or mock digestion (protocol without RNase R ...
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bioRxiv - Bioengineering 2022Quote: ... the purified RNAs were digested by RNase R exoribonuclease (Lucigen, RNR07520) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Ribosomal RNA was removed using the Ribo-Zero Magnetic Kit (Epicentre). RNA fragmentation ...
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bioRxiv - Genomics 2021Quote: ... DNase-treated RNA was subjected to terminator digestion (TEX enzyme, Epicentre) to degrade 5’-monophosphate RNA (16S and 18S ribosomal RNA) ...
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bioRxiv - Genomics 2022Quote: ... we treated the RNA with 10X Baseline-ZERO DNase (Lucigen, DB0715K). DNase I treated RNA samples were purified and further concentrated using RNA clean and concentrator-5 (Zymo Research ...
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bioRxiv - Genomics 2022Quote: ... 5 μg RNA were digested with 1 U RNase R (Epicentre) per μg RNA in a total reaction volume of 10 μl for 10 min at 37°C ...
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bioRxiv - Genetics 2024Quote: Total RNAs were treated with exoribonuclease R (RNase R, Epicentre, RNR07250) to destruct lineRNAs ...