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Citations for Lucigen :
101 - 150 of 386 citations for 5M Betaine Solution PCR Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... genomic DNA extracted (QuickExtract™ DNA Extraction Solution, Lucigen, cat. QE09050) and the remainder restimulated with fresh medium and cultured for an additional 14 days (Jones et al. ...
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bioRxiv - Genetics 2024Quote: ... media aspirated and 30uL of QuickExtract™ DNA Extraction Solution (Lucigen) added to the wells ...
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bioRxiv - Physiology 2024Quote: ... genomic DNA was isolated with the QuickExtract DNA Extraction Solution (Lucigen). The genomic region targeted with the guide RNA was amplified by PCR (Forward primer sequence ...
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bioRxiv - Molecular Biology 2024Quote: ... Genomic DNA was extracted with QuickExtract™ DNA Extraction Solution (Lucigen) and the region of interest was amplified by PCR using Phusion High Fidelity DNA Polymerase (Thermo Fisher) ...
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bioRxiv - Bioengineering 2024Quote: ... and genomic DNA was extracted with QuickExtract DNA Extraction Solution (Lucigen).
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bioRxiv - Biochemistry 2020Quote: ... The standard PCR reaction was performed with the HNqPCRrev1 and HNqPCRfrw1 primers using the Failsafe PCR system with the Premix A (Lucigen, catalog # F599100). Positive and negative PCR control reactions with or without 50ng of HNDNA ...
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A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosabioRxiv - Bioengineering 2023Quote: ... and primers outlined in Table S1. E. coli colony PCRs (Fig. 1F and Fig. S1F- H) were performed with CloneID 1X Colony PCR Mix (Lucigen 30059-2) and primers outlined in Table S1.
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bioRxiv - Developmental Biology 2021Quote: Founder mice were genotyped using FailSafe PCR Kit (EpiCentre) and run on a 12% polyacrylamide gel for heteroduplex assay ...
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bioRxiv - Microbiology 2024Quote: ... FailSafe®PCR Enzyme Mix (Epicentre, Madison, WI, USA) and ScriptSeq®Index PCR Primers (Epicentre ...
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bioRxiv - Bioengineering 2023Quote: ... The PCR product was in vitro transcribed (Epicentre, ASF3507) under the control of promoter T7 for 12 hours at 37°C and DNAse treated with TURBO-DNAse for 20 min at 37°C ...
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bioRxiv - Genomics 2020Quote: Genomic DNA was extracted using QuickExtract™ DNA Extraction Solution (Lucigen, USA). Genomic PCR was performed using 100 ng genomic DNA ...
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bioRxiv - Molecular Biology 2020Quote: ... HSPCs were harvested and QuickExtract DNA extraction solution (Epicentre, Madison, WI, USA) was used to collect gDNA ...
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bioRxiv - Molecular Biology 2020Quote: ... HSPCs were harvested and QuickExtract DNA extraction solution (Epicentre, Madison, WI, USA) was used to collect gDNA ...
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bioRxiv - Neuroscience 2020Quote: DNA was lysed with 50 μl QuickExtract™ DNA Extraction Solution (Lucigen). Copy numbers were analyzed by quantitative real-time PCR performed in an ABI Prism 7900 HT Fast Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Neuroscience 2021Quote: ... tail samples were lysed in QuickExtract DNA Extra Solution 2.0 (Lucigen, USA) and PCR reactions were carried out with GoTaq DNA polymerase and with the Primers ...
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bioRxiv - Neuroscience 2020Quote: Genomic DNA was extracted with QuickExtract™ DNA Extraction Solution (Epicentre, QE09050). Genomic PCR was carried out using Q5® High-Fidelity DNA Polymerase (New England Biolabs ...
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bioRxiv - Cancer Biology 2022Quote: DNA was extracted from cells using the QuickExtract DNA Extraction Solution (Lucigen) and sequencing was performed with 110x coverage using 100 base pair paired end read lengths ...
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bioRxiv - Biophysics 2021Quote: ... coli strain DH5α was isolated using the QuickExtract DNA extraction solution (Lucigen). The coding sequences of the four autoinducer-2 exporter genes were amplified using the Phusion DNA polymerase (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... together with 1 1l genomic DNA in QuickExtract DNA Extraction Solution (Lucigen). After droplet generation with the QX200 Droplet generator (Biorad) ...
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bioRxiv - Developmental Biology 2022Quote: ... DNA for genotyping was extracted using QuickExtract DNA Extraction Solution (Lucigen, QE09050) from harvested yolk sac tissue if available or else from a micro-dissected nick of the extraembryonic anterior proximal region ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA was extracted from one plate using QuickExtract DNA extraction solution (Epicentre) to perform genotyping by PCR using primer pairs specifically recognizing the mutated sequences introduced by the ssODN and a genomic sequence adjacent to the region of integration ...
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bioRxiv - Immunology 2020Quote: ... and resuspended in 50 µL QuickExtract DNA extraction solution (LuciGen, Middleton, WI). The suspensions were transferred to 96-well PCR plates and incubated at 65 °C for 20 min and then at 98 °C for 5 min using a thermocycler ...
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bioRxiv - Molecular Biology 2022Quote: ... Genomic DNA was extracted using QuickExtract™ DNA Extraction Solution (Lucigen #QE09050) and 1 μl (qsp ...
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bioRxiv - Molecular Biology 2022Quote: ... Genotyping was performed by using QuickExtract™ DNA Extraction Solution (Lucigen #QE09050) on a fraction of a clone ...
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bioRxiv - Neuroscience 2024Quote: DNA was extracted from phNPCs with the QuickExtract DNA Extraction Solution (Lucigen). DNA lysate was used as genotyping PCR template with Q5 Hot Start High-Fidelity 2X Master Mix (NEB ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA was harvested from cell pellets using QuickExtract DNA Extraction Solution (Lucigen). The ROS1 kinase and C-terminal domains were PCR amplified using the primers ROS1 5707F (5’-GACAAAGAGTTGGCTGAGCTG-3’ ...
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bioRxiv - Cell Biology 2023Quote: ... and genomic DNA was collected using QuickExtract DNA extraction solution (Lucigen, #QE09050). The region surrounding the targeted site was PCR-amplified (F ...
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bioRxiv - Biochemistry 2024Quote: ... Genomic DNA was extracted using 50µL QuickExtract DNA Extraction Solution (Lucigen, Qiagen). DNA sequences surrounding the deletion site were amplified using the following primers ...
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bioRxiv - Genetics 2023Quote: ... and genomic DNA was extracted using QuickExtract DNA Extraction Solution (QE09050; Epicentre) following the protocol recommended in the Alt-R genomic editing detection kit (1075931 ...
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bioRxiv - Developmental Biology 2023Quote: Tissue from resulting animals was lysed using QuickExtract DNA Extraction Solution (Epicentre) to release the genomic DNA ...
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bioRxiv - Cell Biology 2023Quote: ... genomic DNA was extracted from each clone using QuickExtract DNA solution (Lucigen) according to manufacturer instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Genomic DNA was isolated with the QuickExtract DNA Extraction Solution (Lucigen, QE09050), and correct PAX6 integration of the appropriate FT construct was confirmed by PCR ...
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bioRxiv - Cell Biology 2023Quote: ... DNA was extracted from clones using QuickExtract™ DNA Extraction Solution (Lucigen) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: Genomic DNA extraction was performed using QuickExtract™ DNA Extraction Solution (Lucigen), and the genomic locus of interest was amplified using AmpliTaq Gold® 360 Master Mix (ThermoFisher Scientific) ...
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bioRxiv - Synthetic Biology 2024Quote: ... HSPCs were harvested and QuickExtract DNA extraction solution (Epicentre, Madison, WI, USA) was used to collect genomic DNA (gDNA) ...
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bioRxiv - Developmental Biology 2024Quote: ... DNA for genotyping was extracted using QuickExtract DNA Extraction Solution (Lucigen, QE09050) from harvested yolk sac tissue ...
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bioRxiv - Molecular Biology 2022Quote: ... Complete cDNAs were sequence-amplified by PCR using KOD One™ PCR Master Mix -Blue- (TOYOBO, Japan) and were cloned into pSMART-LCK plasmid (Lucigen, Middleton, WI, USA) containing a T7 RNA polymerase promoter ...
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bioRxiv - Genomics 2020Quote: ... media was aspirated and 50-100 μL QuickExtract™ DNA Extraction Solution (Lucigen) were added to each well ...
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bioRxiv - Immunology 2020Quote: ... DNA was extracted 48 hrs after electroporation using QuickExtract DNA Extraction Solution (Epicentre). Genomic DNA was extracted in the same way from NK cells for off-target studies comparing WT and HiFi Alt-R Cas9 RNP complexes with editing mediated by optimized delivery conditions ...
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bioRxiv - Microbiology 2020Quote: Saliva samples were treated with the Quick ExtractTM DNA Extraction Solution (QE, Lucigen) by mixing 50 μl of saliva with 50 μl of the QE reagent and heating for 5 minutes at 95°C ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were expanded and genomic DNA extracted (QuickExtract DNA Extraction Solution; Epicentre, #QE09050), as described above ...
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bioRxiv - Genomics 2022Quote: Two hundred microliters of lysozyme solution (50 U/μL Ready-lyse lysozyme [Lucigen, WI ...
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bioRxiv - Biophysics 2022Quote: ... DNA from these cells was extracted using QuickExtract DNA extraction solution (Lucigen # QE09050) and used as a template for confirmation of homologous recombination by PCR and Sanger sequencing.
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bioRxiv - Bioengineering 2022Quote: DNA was extracted from harvested cells using the QuickExtract DNA Extraction Solution (Lucigen) according to the manufacturer’s instructions ...
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KIF24 controls the clustering of supernumerary centrosomes in pancreatic ductal adenocarcinoma cellsbioRxiv - Cell Biology 2022Quote: ... Genome DNA was extracted from survival cells using QuickExtract DNA Solution 1.0 (Epicentre), and amplified PCR products using primers listed in Table S1 were sub-cloned into pGEM-T Easy (Promega) ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were re-suspended in 20 μl of DNA Extraction Solution (Lucigen), and incubated at 68°C for 6 min ...
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bioRxiv - Developmental Biology 2020Quote: ... and Sanger sequencing was then extracted using QuickExtract™ DNA Extraction Solution (Lucigen). The PDX1 knockout line was generated in an analogous way.
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bioRxiv - Molecular Biology 2020Quote: Harvested cells were lysed using QuickExtract DNA Extraction Solution (Lucigen, Cat. No. QE09050) following manufacturers protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... the genomic DNA of each clone was extracted by using QuickExtract solution (Epicentre) and PCR was carried out ...
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bioRxiv - Bioengineering 2020Quote: Genomic DNA was extracted with QuickExtract DNA Extraction Solution (catalog no. QE09050; Lucigen, Biosearch technologies ...