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Citations for Lucigen :
1 - 50 of 386 citations for 5M Betaine Solution PCR Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... 10uL Betaine (Lucigen, F881901-1), 5uL random primer mix (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... 2.5 μL betaine (0.8 M; Lucigen, Middleton, WI), 1 μL MgSO4 (4 mM ...
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bioRxiv - Cancer Biology 2021Quote: ... tissues from secondary GBM (Grade III (n=44), Grade IV (n=23)) and LGG (grade II, n=42) using MasterPure kit (Epicentre). Raw reads from the RNA-seq data were processed using an in-house pipeline that uses STAR for read alignment ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA pellets were washed once in 80% ethanol and pellets were air-dried and dissolved in 11uL of water, which was then circularized with the addition of 9uL of CircLigase I mix (1M betaine, 1x CircLigase Buffer (Lucigen), 2.5mM MnCl2 ...
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bioRxiv - Genetics 2021Quote: ... DNA extraction from 2 x 2 x 2mm fin-tissue clips was performed in a 96-well PCR plate using 100μl of QuickExtract solution (Lucigen, USA) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2024Quote: ... Single cell–derived clonal cell lines were analyzed and genotyped by PCR using genomic DNA isolated with QuickExtract DNA Extraction Solution (Lucigen) and primers binding within and downstream the modified region (Primer 1 ...
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bioRxiv - Molecular Biology 2023Quote: ... Single clones were expanded and genotyped for the desired knock-in by PCR on genomic DNA extracted with Quickextract solution (Lucigen). Clones showing biallelic knock-ins were expanded and frozen in LN2 at early passages ...
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bioRxiv - Neuroscience 2021Quote: ... adding CopyControl solution (EpiCentre) 2 hrs before the miniprep ...
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bioRxiv - Genetics 2020Quote: ... PCR was performed using EconoTaq and associated PCR reagents (Lucigen) with 4 μL of crude DNA lysate created as described previously 24 from ear punch biopsy specimens of 8 to 14-day-old mice ...
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bioRxiv - Cell Biology 2024Quote: ... PCR was performed with Failsafe PCR 2X Mastermix (Lucigen, FSP995H), Failsafe Enzyme (Lucigen ...
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bioRxiv - Molecular Biology 2020Quote: ... Genotyping PCR was performed using Failsafe PCR 2x PreMix H (Lucigen) and Taq polymerase (NEB).
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A rapid, highly sensitive and open-access SARS-CoV-2 detection assay for laboratory and home testingbioRxiv - Molecular Biology 2021Quote: ... gargle solution or sputum sample were mixed 1:1 with 2x QuickExtract DNA extraction solution (Lucigen) and heat inactivated for 5 minutes at 95°C ...
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bioRxiv - Molecular Biology 2020Quote: Quick extract DNA extraction solution (Lucigen) was tested in accordance with the manufacturer’s suggested buffer to sample ratio ...
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bioRxiv - Genetics 2021Quote: ... 25 μM ATP standard solution (Epicentre) was used for preparing a concentration gradient (0 ...
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bioRxiv - Immunology 2023Quote: ... 100-200 μL QuickExtract solution (Epicentre) was directly added to cells and incubated at 65 °C for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... QuickExtract DNA Extraction solution from Lucigen; EDTA-free Complete Protease Cocktail from Roche ...
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bioRxiv - Immunology 2024Quote: ... QuickExtract DNA Extraction Solution 1.0 (Epicentre) was used to generate bulk gDNA from dissociated cells to validate editing efficiency before clonal selection ...
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bioRxiv - Microbiology 2021Quote: ... PCR was performed using gene specific primers and EconoTaq PCR Master Mix (Lucigen) per the manufacturer’s guidelines (18 cycles for S14 and 24 cycles for U6) ...
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bioRxiv - Microbiology 2022Quote: ... 2 ml/L fosmid autoinduction solution (Epicentre), each plate supplemented with 0.3% (v/v ...
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bioRxiv - Neuroscience 2023Quote: QuickExtract™ DNA Extraction Solution (QE09050, Lucigen) was used to extract gDNA from the subclones for PCR ...
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bioRxiv - Molecular Biology 2023Quote: ... 50 μL DNA QuickExtract solution (Lucigen QE09050) was added ...
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bioRxiv - Molecular Biology 2024Quote: ... 50 μl QuickExtract DNA Extraction Solution (Lucigen) was used following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... or QuickExtract DNA extraction solution (Lucigen, #QE0905T) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... or QuickExtractTM DNA Extraction Solution (Lucigen, QE09050). A 601 bp genomic region (chr11:92,975,259- 92,975,860 ...
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bioRxiv - Microbiology 2020Quote: ... PCR-free library preparation (Lucigen) and NextSeq 500 sequencing (Illumina ...
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bioRxiv - Genomics 2021Quote: ... The libraries were amplified with 10 PCR cycles using the FailSafe PCR enzyme (Illumina/Epicentre). Libraries were quality controlled on a TapeStation 2200 HSD1000.
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bioRxiv - Biochemistry 2021Quote: ... DNA was extracted (DNA extraction solution, Epicentre Biotechnologies) and edited regions were specifically amplified by PCR (primers are listed in Supplementary Table 1) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... QuickExtract DNA Extraction Solution was purchased from Lucigen Corporation (WI ...
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bioRxiv - Microbiology 2021Quote: ... resuspended a 0.5 mL ReadyLyse lysozyme solution (Epicentre), and then sample incubated for one hour at 37°C with shaking ...
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Unraveling the functions of uncharacterized transcription factors in Escherichia coli using ChIP-exobioRxiv - Systems Biology 2021Quote: ... and lysed with Ready-lyse lysozyme solution (Epicentre). Lysates were sonicated using a sonicator (QSonic ...
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bioRxiv - Neuroscience 2020Quote: ... gDNA was extracted using DNA QuickExtract Solution (Lucigen) followed by PCR and Sequencing to determine efficient rescue.
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bioRxiv - Microbiology 2021Quote: ... 50 U/μL Ready-Lyse Lysozyme Solution (Epicentre), 2 U/mL Zymolyase (Zymo research) ...
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bioRxiv - Molecular Biology 2021Quote: Cells were harvested using Quick Extract solution (Lucigen) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... gDNA was extracted using DNA QuickExtract Solution (Lucigen), followed by PCR and Sanger sequencing to determine efficient repair of the mutation.
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bioRxiv - Microbiology 2022Quote: ... 50 U/μL Ready-Lyse Lysozyme Solution (Lucigen), 2 U/mL Zymolyase (Zymo Research Corporation) ...
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bioRxiv - Microbiology 2022Quote: ... and 10,000 units of ReadyLyse Lysozyme solution (Lucigen). Samples were subjected to bead beating for ten minutes at maximum speed on a vortex mixer with 0.5 mm glass beads (MoBio) ...
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bioRxiv - Molecular Biology 2022Quote: Genomic DNA was extracted using Quickextract solution (Lucigen). A ∼700 bp-1 kb region flanking the sgRNA annealing site was amplified by PCR 5-7 days after RNP nucleofection ...
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bioRxiv - Immunology 2024Quote: Genomic DNA was isolated using QuickExtract solution (Lucigen). Reactions were incubated for 10 min at 65 °C followed by 5 min at 98 °C and cooling to 4 °C ...
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bioRxiv - Immunology 2024Quote: ... Cell pellets were lysed by QuickExtraction solution (Lucigen) per the manufacturer’s instruction at 65°C for 15 min ...
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bioRxiv - Bioengineering 2024Quote: Cells were harvested using Quick Extract solution (Lucigen). Amplicons were generated with Phusion Flash High-Fidelity 2x Mastermix (F548 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... the QuickExtract™ DNA Extraction Solution method (Lucigen), or a salt extraction method (Aljanabi & Martinez ...
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bioRxiv - Genomics 2022Quote: ... 1 μl of 10× stop solution (TNP92110, Lucigen) was added to the reaction mixture to stop tagmentation ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 1 μL/mL Ready-Lyse Lysozyme Solution (Lucigen) and 1 μL/mL benzonase nuclease (Sigma) ...
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bioRxiv - Bioengineering 2023Quote: ... then incubated with 100 μL QuickExtract solution (Lucigen CorporationSupplier Diversity Partner QuickExtract DNA Extraction Solution 1.0 ...
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bioRxiv - Microbiology 2024Quote: ... 1 μL/mL Ready-LyseTM Lysozyme Solution (Lucigen) and 1 μL/mL benzonase nuclease (Sigma) ...
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bioRxiv - Molecular Biology 2024Quote: ... genomic DNA was extracted using QuickExtract solution (Lucigen) according to the manufacturer’s instructions and PCR of CBP C-terminal region was carried out using primers CBP_C-T_fwd1/2 ...
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bioRxiv - Genetics 2024Quote: ... DNA was isolated using QuickExtract DNA Solution (Lucigen) and amplicons were generated using 15 cycles of PCR to introduce Illumina sequencing primer binding sites and 0-8 staggered bases to ensure library diversity ...
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bioRxiv - Microbiology 2022Quote: ... 1 μL/mL Ready-Lyse™ Lysozyme Solution (Lucigen) and 1 μL/mL benzonase nuclease (Sigma) ...
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bioRxiv - Microbiology 2020Quote: ... and 0.1 µl Ready-Lyse™ Lysozyme solution (Epicentre) and incubated for 1 hour at 37°C ...
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bioRxiv - Genetics 2020Quote: DNA was isolated using QuickExtract DNA extract solution (Lucigen) for PCR grade DNA or the QIAamp DNA mini kit (Qiagen ...