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Citations for Agilent :
451 - 500 of 1345 citations for ssc mir 15a RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... slides were washed again and incubated for 30 min at RT with Envision+ HRP labelled polymer anti-mouse (DAKO). Subsequently ...
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bioRxiv - Microbiology 2023Quote: ... pLAIΔEnvGFP.Gag-LUC RT D185E and INT D116N were generated by site-directed mutagenesis using Pfu Turbo DNA polymerase (Agilent) and the following primers:
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bioRxiv - Neuroscience 2023Quote: ... cDNA of the first RT reaction as well as the final library was QC’ed via High Sensitivity DNA chip bioanalyzer (Agilent).
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bioRxiv - Biophysics 2021Quote: ... with help of complementary primers containing mutations (Eurofins MWG Operon, Germany) using the QuikChange protocol for site-directed mutagenesis (Stratagene). Sanger sequencing either outsourced to SourceBioscience (Nottingham ...
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bioRxiv - Biochemistry 2021Quote: ... The RNA was sheared and reverse transcribed with N6 random primers to obtain cDNA library and then qualified by Agilent 2100 Bioanalyzer ...
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bioRxiv - Cell Biology 2019Quote: ... Point mutations were made with complementary primers (listed below) in pTRCHisA-padi2 vectors using QuikChange II Site-Directed Mutagenesis Kit (Agilent).
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bioRxiv - Biochemistry 2022Quote: ... The H335A mutation that renders PLC-γ1 catalytically inactive was introduced into the transfer vector encoding wild-type PLC-γ1 (residues 21-1215) or PLC-γ1 (D1165H) using standard primer-mediated mutagenesis (Agilent Technologies ...
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bioRxiv - Genetics 2019Quote: ... Each of the 210 RHO variants was created via site-directed mutagenesis using a one-primer modification to the QuikChange II protocol from Agilent.((Braman ...
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bioRxiv - Molecular Biology 2019Quote: ... the membranes were incubated with [α-32P]dCTP radioactively labelled DNA probes (Prime-IT RmT Random Primer Labelling Kit, Stratagene) overnight at 65°C ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the CMV enhancer was amplified with the primers P322 and P323 using the phrGFPII-1 plasmid (Agilent Technologies, Santa Clara) as template ...
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bioRxiv - Biochemistry 2020Quote: ... Selected mRNAs were purified and reverse-transcripted into single-chain DNA with the primer 5’-CTTCAGTTGCCGCTTTCTTTCTTG-3’ using a reverse transcriptase (Cat 200436, Agilent). The resulting cDNA library was purified using a DNA purification kit (Cat A740609.25 ...
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bioRxiv - Immunology 2021Quote: ... Product was amplified and barcoded with adaptor specific primers and the quality of the resulting libraries were determined by Agilent Bioanalyzer ...
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bioRxiv - Systems Biology 2022Quote: ... to remove larger DNA fragments and primer dimers and quality was assessed by Bioanalyzer (Agilent 2100, High Sensitivity DNA kit). Samples were pooled according to indexes for Illumina sequencing.
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bioRxiv - Molecular Biology 2020Quote: ... were introduced using RE specific primers (Table 1 and Table 2 in supplementary material) by QuickChange Multi site-directed mutagenesis kit (Agilent) according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2019Quote: ... Input samples were dephosphorylated and 3’-linker ligated to a custom oligonucleotide primer and all samples (IPs and SMInputs) reverse transcribed using AffinityScript reverse transcriptase (Agilent). After treatment with ExoSAP-IT (Affymetrix ...
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bioRxiv - Cancer Biology 2019Quote: ... pGL3-DARPP-32 plasmids were used as templates in PCR reactions to synthesize plasmids containing mutated ASCL1 binding sites by annealing and amplifying site-directed mutagenesis primers using QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent). Digestion with Dpn I enzyme was carried out to eliminate methylated parental plasmid strands ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Transferred DNA was UV crosslinked to the membrane and hybridized with a 32P-labeled (Prime-It II Random Primer Labeling Kit, Agilent) Lpar1 probe with sequence external to the 5’ end of the targeting vector ...
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bioRxiv - Developmental Biology 2019Quote: ... pBMN-HIPK4-Y175F-3xFLAG-IRES-mCherry was later generated by site-directed mutagenesis of the wild type construct using the primers 5’-CGCTATGTGAAGGAGCCTTTCATCCAGTCCCGCTTC TAC-3’ and 5’-GTAGAA GCGGGACTGGATGAAAGGCTCCTTCACATAGCG-3’ and PfuUltra II Fusion polymerase (Agilent).
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bioRxiv - Cancer Biology 2021Quote: ... pCMVHA hEZH2 and V5-EZH2 vector was used to generate EZH2-H689A mutant vector using the mutagenesis primers listed in Table S1 with QuikChange II site-directed mutagenesis kit (Agilent) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... USP47 catalytic domain was mutated by changing cysteine 177 into alanine (C177A) using the sense primer (5’-gtttgcaaaaggctattcaaataggcagtcattgcttggtttactagtcc-3’) using Quickchange Lightning Site-directed Mutagenesis Kit from Agilent technologies ...
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bioRxiv - Microbiology 2023Quote: ... and a barcoding primer (unique for each sample to allow for demultiplexing of reads) with the Easy A cloning kit (Agilent). A 200–400 bp product was gel-purified and sequenced on an Illumina NextSeq Platform (in lab ...
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bioRxiv - Molecular Biology 2022Quote: ... Point mutations in the repM gene were generated using specific primers and a QuikChange Site-Directed Mutagenesis kit according to the protocol supplied by the manufacturer (Stratagene). All oligonucleotide primers used in this study are listed in Supplementary Table S2.
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bioRxiv - Microbiology 2023Quote: ... along with gene-specific primers listed in Supplemental Table 3 and qPCR was performed in technical triplicate on a StepOnePlus (Stratagene). Ct values of target genes were normalized to β-actin and relative gene expression levels between conditions were calculated via the ΔΔCt method ...
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bioRxiv - Cancer Biology 2023Quote: ... Gene-specific primers were used (Table S1) together with Brilliant III Ultra-Fast SYBR Green QPCR Master Mix (Agilent Technologies), following the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2023Quote: ... pooled libraries were additionally purified with AMPure beads (ratio 1:1) to remove contaminating primer dimers and quantified using Qubit and the Bioanalyzer High-Sensitivity DNA kit (Agilent). 50-bp paired-end deep sequencing was carried out on HiSeq 4000 (Illumina).
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Development of a genetically-encoded sensor for probing endogenous nociceptin opioid peptide releasebioRxiv - Neuroscience 2023Quote: ... site directed mutagenesis and Circular Polymerase Extension Cloning was performed by polymerase chain reaction with custom designed primers using a Pfu-Ultra II fusion High Fidelity DNA Polymerase (Agilent). Sanger sequencing (Microsynth ...
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bioRxiv - Plant Biology 2023Quote: CDS sequences from PhDEF and PhGLO1 were amplified from Petunia x hybrida R27 inflorescence cDNAs with primers MLY2382/MLY2383 and MLY2384/2385 respectively (Supplementary Table 1) and cloned into the in vitro translation vector pSPUTK (Stratagene) by NcoI/XbaI restriction ...
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bioRxiv - Plant Biology 2023Quote: ... the first-strand complementary DNA (cDNA) was synthesized from 1 μg of RNA using oligo (dT) primers with Reverse Transcriptase (Agilent). Quantitative reverse transcription polymerase chain reaction (qRT-PCR ...
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bioRxiv - Microbiology 2023Quote: ... primer annealing and extension at 58°C for 30 s followed by dissociation step in a thermal cycler (Stratagene Mx3005P). Finally ...
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bioRxiv - Biochemistry 2023Quote: ... The NopD point mutants constructs were generated by different primers and were created by the QuickChange site-directed mutagenesis kit (Stratagene). All primers are shown in the Supplementary Table1 ...
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bioRxiv - Genetics 2024Quote: Membranes were hybridized with “probe 4” for the HIS4::LEU2 hotspot 100 which was 32P-dCTP labelled using the Prime-It II Random Primer Labeling Kit (Stratagene). Following hybridization ...
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bioRxiv - Microbiology 2024Quote: ... The E50A variant was further obtained by site-directed mutagenesis using an adapted protocol from the Stratagene “QuickChange” with only one mutator primer carrying the modified sequence and with both the Pfu turbo polymerase (Stratagene) and the Taq ligase (New England Biolabs ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were then assessed for appropriate amplicon size and complete elimination of free primer peaks using the High Sensitivity ScreenTape Assay on the Tape Station2200 (Agilent) and quantified using the qPCR-based quantification in order to ensure only NGS-compatible amplicon was quantified using the Library Quant ROX Low Kit (Kapa Biosystems ...
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bioRxiv - Molecular Biology 2024Quote: ... The quality and yield of fully-barcoded DNA primers was evaluated with total RNA Pico Assay (Agilent Technologies, 5067-1513) and by fluorescent in situ hybridization (FISH ...
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bioRxiv - Cell Biology 2020Quote: ... PCR products were then gel extracted and cloned into a vector using the Strataclone PCR Cloning kit (Agilent, Santa Clara, CA) and Sanger sequenced using the BigDye Terminator Cycle sequencing kit (Applied Biosystems ...
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bioRxiv - Bioengineering 2021Quote: ... Mutations were cloned into the expression vector by polymerase chain reaction (PCR) using a PFUultra II Hotstart PCR Master Mix (Agilent Technologies). The obtained PCR products were subsequently treated with DPNI (New England Biolabs ...
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bioRxiv - Cancer Biology 2021Quote: ... The DNA was denatured at 95°C for 5 min in a PCR machine (PTC-200, MJ Research; PCR strip tubes (Agilent 410022)) and then incubated with the biotin capture RNA at 65°C ...
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bioRxiv - Cell Biology 2021Quote: ... or IRBITKO cells was subjected to PCR amplification (Herculase II Fusion DNA Polymerase and 5X Herculase II PCR Buffer and dNTP (Agilent Techologies) using primers flanking the region targeted by the gRNA ...
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bioRxiv - Immunology 2020Quote: ... qRT-PCR for mRNA expression was done with the Brilliant III Ultra-Fast SYBR Green qRT-PCR Master Mix (Agilent Technologies) as previously described (19) ...
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bioRxiv - Cancer Biology 2023Quote: ... The qPCR was performed in 20 μl SYBR Green reaction mixture using a Real-time PCR system (Agilent AriaMx Real-time PCR System, Agilent, USA). The ChIP experiments and qPCR were performed in triplicates ...
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bioRxiv - Cancer Biology 2023Quote: ... The fragment was gel purified and used as template in error-prone PCR (EP-PCR) using the GeneMorph II kit and Mutazyme II polymerase (Agilent Technologies) and the primers (5RT 3RT) ...
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bioRxiv - Cell Biology 2020Quote: ... pEGFP-C1-tubbyCT KR330/332AA was generated by mutagenesis PCR using PfuUltra II Hotstart PCR Master Mix (Agilent Technologies, Santa Clara, US).
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bioRxiv - Genetics 2023Quote: ... and Illumina adapters were added (second PCR) in a nested PCR manner using Agilent Herculase II Fusion DNA Polymerase Kit (Agilent, Cat # 600679). The PCR products were purified and sequenced using an Illumina NextSeq ...
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bioRxiv - Molecular Biology 2024Quote: ... sgRNA-encoding genomic loci were amplified from up to 400 µg gDNA via PCR using a Herculase II Fusion Polymerase PCR kit (Agilent, Cat. #600677). Pooled reaction products from each treatment group were uniquely barcoded in a subsequent PCR reaction followed by electrophoresis on 2% TBE-agarose gel at 120 V ...
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bioRxiv - Immunology 2022Quote: ... for 1 hour RT and replacing the secondary antibody incubation with HRP-labeled polyclonal goat α-mouse antibody (Dako K4000) for 30 minutes RT ...
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bioRxiv - Genetics 2019Quote: ... Isolated RNA was reverse transcribed into cDNA was by Prime Script TM RT reagent Kit with gDNA Eraser (Stratagene, Takara). Real-time PCR was performed on ABI-7500 real-time PCR machine (Applied Biosystems ...
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bioRxiv - Cell Biology 2021Quote: ... RNA concentration was measured by Nanodrop and 1 to 3 μg of RNA was reverse transcribed with AffinityScript Multi-Temp RT (Agilent) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... and retention time (RT) were imported into a personal compound database and library (PCDL Manager, version B.07.00, Agilent Technologies) used in data processing workflow.
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bioRxiv - Biochemistry 2021Quote: ... AccuScript high-fidelity first-strand cDNA synthesis kit for reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) was procured from Agilent.
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bioRxiv - Neuroscience 2022Quote: ... membranes were incubated for 1h at RT with the secondary antibody (Polyclonal Goat Anti-Rabbit Immunoglobulins/HRP, Dako; Polyclonal Goat Anti-Mouse Immunoglobulins/HRP, Dako) prepared in the same blocking solution at a dilution of 1:10.000 ...