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Citations for Agilent :
251 - 300 of 1345 citations for ssc mir 15a RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... RT-qPCR analyses were performed using Brilliant III Ultra-Fast SYBR® Green (Agilent) after DNase treatment using Turbo DNase (AMBION) ...
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bioRxiv - Bioengineering 2021Quote: ... were applied for 30 mins at RT and samples were exposed DAB substrate (Dako) for 5 mins ...
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bioRxiv - Neuroscience 2020Quote: ... then 2h at RT with a rabbit IgG anti-S100β antibody (1:250; Dako-Agilent ...
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bioRxiv - Cell Biology 2020Quote: ... RT-qPCR assays were analyzed with 2(-ΔΔCt) method 85 via MxPro software (Stratagene) and expressed as relative quantity to normalizer 86.
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bioRxiv - Genetics 2022Quote: ... cDNA was synthesized from 150 ng of total RNA with AffinityScript RT buffer (Agilent) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... at RT for 1hr and stained with primary antibodies diluted in Antibody Diluent (Dako) overnight at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... quantitative reverse transcriptase PCR (qRT-PCR) was performed with the Brilliant III Ultra Fast SYBR® Green QRT-PCR Mastermix (Agilent, Waldbronn, Germany): 5 µl Master Mix (supplied) ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR products of positive clones were cloned with the StrataClone PCR Cloning Kit (Agilent) into the supplied vector system ...
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bioRxiv - Cell Biology 2022Quote: ... The PCR reactions were carried out in an AriaMx Real-time PCR System (Agilent). For absolute quantification ...
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bioRxiv - Microbiology 2021Quote: ... The PCR reaction was performed on a Stratagene MX3005p quantitative PCR instrument (Stratagene, USA). Results were expressed as Log10 FMDV RNA copies/108 copies 18S.
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bioRxiv - Biochemistry 2023Quote: ... PCR products were cloned using the StrataClone PCR cloning kit (Cat #240205, Agilent Technologies) and sequenced using T3 and T7 oligonucleotide to confirm absence of wild type allele.
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bioRxiv - Systems Biology 2024Quote: ... PCR products were cloned into TOPO vector (Strataclone Blunt PCR Cloning Kit, Agilent 240207) and selected on agar plates with ampicillin and kanamycin ...
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bioRxiv - Evolutionary Biology 2024Quote: ... PCR products were cloned using a StrataClone Blunt PCR Cloning Kit (Agilent, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... labelled with [α-32P]-dCTP through the Prime-it II random primer labelling kit (Stratagene) was used ...
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bioRxiv - Plant Biology 2019Quote: ... The probes were prepared using Prime-It® Rm T Random Primer Labeling kit (Stratagene). The probe for AtMIOX4:pBIB-Kan was amplified with npt-II forward (5’-AGA GGC TAT TCG GCT ATG AC-3’ ...
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bioRxiv - Immunology 2019Quote: ... we used the following mutagenesis primers using the QuikChange II Site-Directed Mutagenesis Kit (Agilent); F ...
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bioRxiv - Biochemistry 2022Quote: ... Site-directed mutations were introduced using the M27-QC primers and the QuikChangeII kit (Agilent) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... with appropriate primers (Table S9) and the Pfu Ultra II Fusion HS DNA (#600674, Agilent) polymerase ...
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bioRxiv - Genetics 2022Quote: ... The primers used for mutagenesis using the Quickchange Lightning mutagenesis kit (Stratagene, La Jolla, CA) are as follows:
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bioRxiv - Evolutionary Biology 2024Quote: ... and primer oVG6_OrV_RNA1_3’_R cDNA of OrV RNA2 was obtained using AccuScript Reverse Transcriptase (Agilent) and primer oVG61_OrV_RNA2_R cDNA complementary to 3’ ends were synthesized from negative viral strands using forward primers ...
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bioRxiv - Plant Biology 2019Quote: ... Data normalization applied to each experiment set of microarrays was dependent on platform technology (RMA for Affymetrix and Quantile Normalization for Agilent).
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bioRxiv - Cancer Biology 2019Quote: ... Peptides were separated on a 75-min linear gradient from 5% to 35% acetonitrile in 10mM ammonium bicarbonate at a flow rate of 0.5mL/min on an Agilent 1260 Infinity pump equipped with a degasser and a diode array detector (set at 214, 220, and 254nm wavelength) from Agilent Technologies (Waldbronn ...
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bioRxiv - Plant Biology 2019Quote: ... Purity and integrity of total RNA was assessed on the Agilent 2100 Bioanalyzer with the RNA 6000 Pico LabChip reagent set (Agilent). RNA extraction from egg cells was carried out at the genomics core facility of the University of Regensburg ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and a representative set of 12 samples were further assayed on using a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA) for fragment length distribution and RNA integrity (RIN) ...
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bioRxiv - Immunology 2019Quote: ... A random set of 10 out of 36 final libraries were quality checked on the High Sensitivity DNA kit (Agilent) that revealed an average fragment size of 400bp ...
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bioRxiv - Molecular Biology 2019Quote: ... Csl4-F121A and Csl4-F129A variants were generated by site-directed mutagenesis of their wildtype counterparts with appropriate sets of oligonucleotides using the QuikChange II XL Kit (Stratagene) as recommended.
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bioRxiv - Neuroscience 2021Quote: ... HPLC separation was performed by Shimadzu LC-20 AD Analytical System using UV (Agilent 1100 VW set at 253 nm) detection ...
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bioRxiv - Microbiology 2022Quote: The tandem mass spectrometer was hyphenated to a liquid chromatography set-up comprising 2 binary pumps (1290 series, Agilent technologies). For each experiment ...
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bioRxiv - Immunology 2024Quote: ... The Dual Index TT Set A (10x Genomics; PN 3000431) was used for the final library preparation and a 2100 Bioanalyzer (Agilent) was used to perform library quality control and concentration quantification ...
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bioRxiv - Cancer Biology 2024Quote: ... The slides were probed with a set of 216 antibodies against total and phospho-proteins using an automated slide stainer Autolink 48 (Dako). Each slide was incubated with one specific primary antibody ...
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bioRxiv - Immunology 2021Quote: ... PCR reactions were performed using Easy-A High-Fidelity PCR master mix (Agilent Technologies, UK) on a Mastercycler Pro Thermal Cycler (Eppendorf ...
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bioRxiv - Molecular Biology 2019Quote: ... Second-round PCR was performed with SYBR Green and real-time PCR conditions (Mx3005p, Agilent) (Brunet et al. ...
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bioRxiv - Plant Biology 2019Quote: ... QRT-PCR assays were performed using an AriaMx Real-Time PCR system (G8830A instrument; Agilent) and the accompanying software (version 1.5) ...
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bioRxiv - Microbiology 2019Quote: ... Both PCR amplifications were carried out using the Paq5000 Hotstart PCR Master Mix (Agilent, USA). The library was sequenced on the MiSeq platform with the MiSeq Reagent V2 250 cycles kit ...
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bioRxiv - Plant Biology 2022Quote: ... and quantitative real-time-PCR was performed on an AriaMx Real-Time PCR System (Agilent) using Absolute Blue Low Rox Mix (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... Error-prone PCR was done using Mutazyme PCR from Genemorph II mutagenesis kit (Agilent Technologies) according to the manufacturer’s suggestions ...
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bioRxiv - Genetics 2022Quote: ... and quantitative real-time-PCRs were performed on an AriaMx Real-Time PCR System (Agilent) using Absolute Blue Low Rox Mix (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... The PCR product was cloned into pSC vector (Strataclone PCR cloning kit Agilent 240205-12) and verified by Sanger sequencing ...
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bioRxiv - Neuroscience 2020Quote: ... then 2h at RT with a rabbit IgG anti-S100β antibody (1:250; Dako-Agilent, cat# Z031101-2 or between 1:4 and 1:5 ...
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bioRxiv - Microbiology 2021Quote: ... at RT for 1 h followed by incubation with streptavidin R-Phycoerythrin (PE, Agilent Technologie) at RT for 30min ...
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bioRxiv - Neuroscience 2019Quote: ... consisting of a 10-minute RT incubation with boiling antigen retrieval solution (Dako, Ely, UK). Slides were washed three times with PBS and blocked for 1 hour with 5% Normal Donkey Serum (NDS ...
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bioRxiv - Neuroscience 2022Quote: ... for 30 min at RT and peroxidase activity was revealed using DAB+ (Dako, K346811-2). Finally ...
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Adolescent parvalbumin expression in the left orbitofrontal cortex shapes sociability in female micebioRxiv - Animal Behavior and Cognition 2023Quote: ... for 2 hours at RT before mounting them in clear mounting medium (DAKO, cat#S3023). We imaged fluorescent signals of mounted brain sections under a scanning microscope (ZEISS ...
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bioRxiv - Cell Biology 2022Quote: ... at RT for 5 min before mounting the glass coverslips using DAKO mounting medium (Agilent). Primary antibodies used herein are listed in Table 2 while secondary antibodies used are ...
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bioRxiv - Cell Biology 2023Quote: ... Tissue sections were blocked for 1 h at RT with protein blocking solution (#X0909, Dako). After blocking ...
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bioRxiv - Neuroscience 2020Quote: ... striatal metabolites were measured with a gas-chromatography/mass-spectrometry set-up (Agilent 7890B GC – Agilent 5977A MSD, Santa Clara, CA). Absolute level of DA were determined using an internal standard ...
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bioRxiv - Biochemistry 2020Quote: ... The column was in line with the following detectors: a variable UV absorbance detector set at 280 nm (Agilent 1100 series), the DAWN EOS MALS detector (Wyatt Technology ...
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bioRxiv - Biochemistry 2020Quote: ... The column was in line with the following detectors: a variable UV absorbance detector set at 280 nm (Agilent 1100 series), the DAWN EOS MALS detector (Wyatt Technology ...
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bioRxiv - Genetics 2020Quote: We enriched for ∼2.3 mB of the MSY from the same genomic libraries for male samples via in-solution hybridization-capture using a previously designed probe set (Kutanan et al. 2018a; 2019) and the Agilent Sure Select system (Agilent, CA). Sequencing was carried out on the Illumina HiSeq 2500 platform with paired-end reads of 125-bp length and we used Bustard for Illumina standard base calling ...
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bioRxiv - Molecular Biology 2023Quote: ... The RNA samples’ quality (purity and integrity) was determined utilizing the Agilent 2100 Bioanalyser with the RNA 600 NanoLabChip reagent set (Agilent Technologies). The RNA was quantified by Qubit fluorometry (Thermo Scientific) ...