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Citations for Agilent :
451 - 500 of 6656 citations for rno mir 16 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... The EnVision Detection System (Agilent) was used for detection ...
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bioRxiv - Immunology 2023Quote: ... The EnVision detection system (Agilent) was used with AEC (Agilent ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were cloned using StrataClone PCR cloning kit (product no: 240205, Agilent technologies Sweden AB, Kista) and re-amplified with M13F/M13R primers using DreamtTaq (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... PCR products were ligated into pSC-A vectors using a Blunt-end PCR cloning kit (Agilent #240207). Ligated plasmids were transformed into XL-1 blue competent cells ...
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative PCR (5 ng cDNA/well) was performed by using QuantiNova kit on Mx3500P PCR machine (Stratagene). Gene-specific primers were used for end-point PCR (HotStarTaq Plus DNA Polymerase ...
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bioRxiv - Synthetic Biology 2020Quote: The PCR-based Quick change site directed mutagenesis kit (Agilent) was used to remove N-glycosylation sites from the hIL-22 ORF by mutating the Asn residue of the Asn-X-Ser/Thr consensus sequence to Gln (Supplementary Table 4) ...
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bioRxiv - Biochemistry 2021Quote: ... Polymerase chain reaction (PCR) reagents and kits were from Agilent and Himedia ...
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SARS-CoV2 associated secretion of nanoLuciferase reports on virus and Virus-Like Particle productionbioRxiv - Molecular Biology 2022Quote: ... A one-step qRT-PCR kit with SYBR green (Agilent) was used with the primer sequences below ...
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bioRxiv - Cell Biology 2023Quote: ... qRT-PCR Brilliant III ultra-fast master mix kit (Agilent) and TaqMan gene expression probes (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... qRT-PCR was performed using Rotor- Gene™ 6000 Real-Time thermocycler (Corbett Research, Australia) with Brilliant SYBR® Green QPCR Master Mix (Stratagene, La Jolla, CA) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: ... Real-time PCR amplification with the 2 x precision master mix (Primer design, Southampton, UK) using the Stratagene Mx3000P real-time QPCR system (Agilent Technologies, Santa Clara, CA, USA). Each sample was tested in triplicate and compared to a housekeeping gene (Atp5B in osteoblasts and bone tissue ...
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bioRxiv - Biochemistry 2020Quote: The rate of debenzylation of 7-benzyloxyquinoline was measured with a real-time continuous fluorometric assay using a Cary Eclipse fluorometer (Agilent Technologies, Santa Clara, CA, USA) or a custom-modified PTI QM-1 fluorometer (Photon Technology International ...
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bioRxiv - Plant Biology 2024Quote: ... Fragmentation (at 60 °C) and hybridization (at 65° C for 16 hours) were done using the Gene Expression Hybridization kit of (Agilent Technologies ...
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bioRxiv - Cell Biology 2020Quote: Real-time oxygen consumption and extracellular acidification rates in C2C12 myoblasts treated with 16% palmitate-BSA (1mM palmitate conjugated to 0.17mM BSA) or 16% BSA (0.17 mM; Seahorse Bioscience #102720-100) were obtained using the XF96 Bioanalyzer (Seahorse Bioscience ...
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bioRxiv - Molecular Biology 2022Quote: ... Detection of bound primary antibody was achieved using Dako EnVision + TM Peroxidase Rabbit Kit (DAKO, Glostrup, Denmark) or Dako EnVision + TM Peroxidase Mouse Kit (DAKO ...
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bioRxiv - Bioengineering 2023Quote: ... were applied followed by detection using an EnVision+System-HRP (DAB) kit (Dako North America, Carpinteria, CA) and hematoxylin counter staining ...
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bioRxiv - Plant Biology 2023Quote: ... The RT-qPCR was carried out using Brilliant III Ultra-Fast SYBR Green qPCR kit (Agilent Technologies) on an AriaMx G8830A Real-Time qPCR System (Agilent Technologies) ...
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bioRxiv - Cell Biology 2020Quote: Adenoviruses for miR-29a and the scrambled miRNA control were cloned using the AdEasy system (Agilent, Cat#240010) according to the protocol provided ...
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bioRxiv - Immunology 2022Quote: ... Knockout PCR products were cloned into pSC-B-amp/kan with the StrataClone Blunt PCR Cloning Kit (Agilent) and Sanger sequenced to determine the exact deletion ...
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bioRxiv - Molecular Biology 2019Quote: Real-time oxygen consumption rates (OCR) and extra-cellular acidification rate (ECAR) were measured with a Seahorse XFe96 Analyzer (Seahorse bioscience Inc., North Billerica, MA, USA) as described44 ...
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bioRxiv - Cancer Biology 2020Quote: ... Signal detection with AEC substrate (DAKO). Sections were counterstained with Hematoxylin and mounted with Aquatex.
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bioRxiv - Plant Biology 2021Quote: ... and the Mx3000P detection system (Stratagene) was used ...
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bioRxiv - Cancer Biology 2020Quote: ... EnVision FLEX HRP detection reagent (Dako) for secondary amplification and enzymatic conjugation ...
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bioRxiv - Microbiology 2020Quote: ... the adapter-ligated/RT primer mixture was used as template with the AffinityScript cDNA Synthesis Kit (Agilent, 600559) with the following reaction conditions ...
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bioRxiv - Immunology 2022Quote: ... MARS-seq barcoded RT primers were used for reverse transcription with the Affinity Script cDNA Synthesis Kit (Agilent) in a 10 μl reaction volume.
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bioRxiv - Cell Biology 2024Quote: ... Total RNA was processed for reverse transcription (RT) using AccuScript High-Fidelity First Strand cDNA Synthesis Kit (Agilent). A mix of anchored-Oligo(dT ...
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bioRxiv - Plant Biology 2019Quote: ... were introduced by the Quick-Change PCR mutagenesis kit protocol (Stratagene), using the primer combinations listed in Supplemental Table 2 and PhusionTM High-Fidelity DNA Polymerase (Finnzymes) ...
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bioRxiv - Cancer Biology 2020Quote: ... Quantitative PCR was performed by using QuantiNova kit on Mx3500P (Stratagene). The oligonucleotide primers used in this study are shown in Supplementary Table 2.
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bioRxiv - Neuroscience 2020Quote: ... error-prone PCR was performed with the GeneMorphII kit (Agilent Technologies) or Taq polymerase (New England Biolabs ...
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bioRxiv - Microbiology 2022Quote: ... A one-step qRT-PCR kit with SYBR green from Agilent was used ...
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bioRxiv - Microbiology 2022Quote: ... vector pDFTT3-CTL0481 was PCR-amplified (QuikChange II XL Kit, Agilent) using primers pDFTT3-rvcat_F and pDFTT3-rvcat_R (Table S2) ...
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bioRxiv - Cancer Biology 2020Quote: PCR reactions were performed using Quik-Change XL kit by Agilent Technologies (catalogue no.200517-4 ...
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bioRxiv - Cell Biology 2020Quote: ... The PCR products of positive clones were then cloned into competent cells using the StrataClone PCR Cloning Kit (Agilent) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting PCR products were subcloned into the holding vector pSC-B (StrataClone Blunt PCR Cloning Kit, Agilent Technologies) and 16 colonies (white ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR amplicons were further cloned into a pSC-amp/kan vector using StrataClone PCR cloning kit (240205, Agilent Technologies). We picked ∼20-50 single colonies per sample to determine the presence ...
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bioRxiv - Developmental Biology 2023Quote: ... Quantitative PCR was performed using the 1-Step Brilliant II SYBR Green QRT-PCR Master Mix kit (Agilent Technologies) and the TaqPath 1-Step Multiplex Master Mix kit (Thermo Fisher Scientific ...
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bioRxiv - Pathology 2023Quote: ... then washed in PBS and exposed to the detection kit (EnVision + System-HRP Anti-Rabbit, Dako Denmark A/S) which included 3,3′-diaminobenzidine tetrahydrochloride as chromogen and counterstained with Harris hematoxylin ...
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bioRxiv - Immunology 2021Quote: ... PCR products were ligated into pSCB-Amp/Kan using the StrataClone Blunt PCR Cloning Kit (Agilent Technologies, La Jolla, CA). The Spic promoter was cloned into pGL3-Basic (Promega ...
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bioRxiv - Neuroscience 2021Quote: ... The 481 bp ywhaz amplicon was generated by PCR reaction and cloned into a plasmid using the StrataClone PCR cloning Kit (Agilent). The plasmids were collected and purified using GeneJET Plasmid Maxiprep Kit (Thermo Scientific ...
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bioRxiv - Genomics 2022Quote: ... RT was performed with a specific primer (5′-CCTACACGACGCTCTTCC-3′) using AffinityScript Multiple Temperature cDNA Synthesis Kit (Agilent Technologies). RNA degradation was performed by incubating the RT mixture with 10% 1 M NaOH (2μl of RT mixture ...
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bioRxiv - Immunology 2020Quote: ... REAL peroxidase Blocking agent (Dako, Agilent) and casein solution (Vector labs ...
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bioRxiv - Immunology 2020Quote: ... REAL peroxidase Blocking agent (Dako, Agilent) and casein solution (Vector labs ...
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bioRxiv - Genomics 2021Quote: ... and 16 μl Herculase II Fusion DNA Polymerase (Agilent Technologies). The reaction conditions for the PCR reaction were 95 °C for 1 minute ...
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bioRxiv - Cancer Biology 2021Quote: ... cytokeratin 5/6 (mouse monoclonal, clone 6D5/16 B4, Dako), epidermal growth factor receptor (EGFR ...
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bioRxiv - Pathology 2023Quote: ... the monoclonal mouse antibody (M7237, Clone D5/16 B4, Dako) was incubated for 30 minutes at room temperature at a 1∶200 dilution followed by Labelled Polymer-HRP Anti-Mouse for 30 minutes ...
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bioRxiv - Genomics 2023Quote: ... or 16 kbp [as determined by FEMTO Pulse (Agilent, M5330AA)] were chosen for sequencing ...
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bioRxiv - Cell Biology 2020Quote: ... PCR mutagenesis was performed using QuickChange II site directed mutagenesis kit (Agilent) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2021Quote: ... to generate pScGFPTUB1 using a Stratagene blunt PCR cloning kit (Agilent Technologies). The neomycin (NEO ...
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bioRxiv - Microbiology 2020Quote: ... Mutagenesis was performed by PCR using the Quikchange mutagenesis kit (Agilent Technologies) and primers specified in Table S2 ...
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bioRxiv - Cancer Biology 2020Quote: ... and genetically authenticated using a STR-PCR fragments kit (Agilent Technologies, USA) as per our previous published technique [37] ...