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Citations for Agilent :
701 - 750 of 6656 citations for rno mir 16 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Parkin mutant plasmids were cloned beginning with a Homo sapiens wild type eGFP-parkin vector [36] and performing PCR point mutagenesis (Quikchange Mutagenesis kit: Stratagene) utilizing primers ordered from Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... the PCR products from the genotyping of the first outcross (generation F1) were cloned into the cloning vectors provided by the StrataClone PCR cloning kit (Stratagene). The cloning was performed according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... 10X-barcoded full-length cDNA was then amplified via PCR and analyzed using Bioanalyzer High Sensitivity DNA kit (5067-4626, Agilent). Up to 50 ng of cDNA was carried over to construct gene expression libraries and was enzymatically fragmented and size-selected to optimize the cDNA amplicon size prior to 5’ gene expression library construction ...
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Role of the Topoisomerase IIα Chromatin Tether domain in Nucleosome Binding & Chromosome SegregationbioRxiv - Cell Biology 2021Quote: ... the ChT swapping mutants using fusion PCR and the point mutations using site-directed mutagenesis by a QuikChangeII kit (Agilent) with the primers listed in supplemental information (Table S1) ...
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bioRxiv - Cancer Biology 2022Quote: ... All K-Ras secondary mutant and wild-type K-Ras constructs were generated by a PCR based strategy using a site-directed mutagenesis kit (Stratagene) according to the manufacturer’s instructions ...
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Species-specific protein-protein interactions govern the humanization of the 20S proteasome in yeastbioRxiv - Genetics 2022Quote: The PSMB7 mutant gene library was previously generated (Kachroo et al, 2015) by error-prone PCR (GeneMorph II Random Mutagenesis Kit from Agilent) to introduce mutations and add attL1 and attL2 sites at the 5’ and 3’ ends of the gene (Reece-Hoyes & Walhout ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 ng of extracted PCR products were analyzed by an Agilent High Sensitivity DNA kit on an Agilent 2100 Bioanalyzer (Agilent), and the remainder was cloned by using PCR Cloning Kit (New England Biolabs ...
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bioRxiv - Neuroscience 2023Quote: ... Amplified DNA from F1 mutants was used for cloning with the StrataClone PCR Cloning Kit (Agilent; Santa Clara, CA, USA) and then transformed with StrataClone SoloPack (Agilent ...
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bioRxiv - Cell Biology 2023Quote: ... The tau P301L mutants were generated by PCR using QuickChange Lightning Site-Direct mutagenesis kit (Agilent Technologies, Santa Clara, USA). For all intermolecular sensors ...
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bioRxiv - Microbiology 2023Quote: ... This DNA was then subject to an initial round of PCR amplification with primers oTB535 and oTB536 with the Easy A cloning kit (Agilent), digested with NotI (NEB ...
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bioRxiv - Cell Biology 2023Quote: ... the pK2:Nβ-GFP construct was used as template for site-directed mutagenesis by PCR using the QuickChange Lightning Site-Directed Mutagenesis Kit (Agilent) following the manufacturer’s instructions with the concomitant mutagenic primers for each target base substitution (S2 Table) ...
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bioRxiv - Biochemistry 2022Quote: ... The N165A and N234A variants of HDM-SARS2-S-del21-D614G were generated by site-directed PCR mutagenesis using the QuickChange II kit (Agilent), and changes were confirmed by sequencing ...
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bioRxiv - Cell Biology 2023Quote: All TTBK2 mutations were generated by PCR-based site-directed mutagenesis by using QuickChange Lighting Site-Directed-Mutagenesis kit (Agilent) using as a template pENTR/D-TOPO (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... The library of CjCas9 variants was produced by random mutagenesis of the wild-type sequence using error-prone PCR approach (GeneMorph II kit from Agilent) (for primers ...
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bioRxiv - Molecular Biology 2023Quote: ... Site directed mutagenesis of pORIPS and pGII was performed by PCR using the QuikChange II Site-directed mutagenesis kit (Agilent).
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bioRxiv - Cancer Biology 2024Quote: ... 10X-barcoded full-length cDNA was then amplified by PCR and analysed using Bioanalyzer High Sensitivity DNA kit (5067-4626, Agilent). Up to 50 ng of cDNA was carried over to construct gene expression libraries and was enzymatically fragmented and size-selected to optimize the cDNA amplicon size prior to 5’ gene expression library construction ...
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bioRxiv - Cell Biology 2021Quote: ... cells were resuspended in DMEM with 10% of FCS and transferred into xCELLigence microplates (E-plate 16, Agilent) at the density of 20 000 cells for real-time analysis of cell adhesion and growth ...
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bioRxiv - Cancer Biology 2021Quote: ... Endogenous peroxidase activity was blocked by incubation with REAL Peroxidase-Blocking Solution (Dako) at room temperature for 10 min ...
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bioRxiv - Immunology 2022Quote: ... Sections were washed and incubated with Dako REAL peroxidase blocking solution (Dako, K0672) to inactivate endogenous peroxidases and unspecific bindings were blocked (PBS/10% FCS).Tissue sections were incubated with rabbit anti-Ki67 antibody (Abcam ...
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bioRxiv - Pathology 2023Quote: ... endogenous peroxidases were quenched with DAKO REAL Peroxidase-Blocking Solution (S202386-2, Agilent) for 5 min ...
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bioRxiv - Cancer Biology 2023Quote: ... sections were incubated with secondary antibody (DAKO REAL™ EnVision ™ Glostrup, Denmark) for 20 min as per the kit instructions ...
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bioRxiv - Microbiology 2020Quote: ... hybridization and detection steps were carried out according to the supplier’s instructions (Agilent Technologies). For each microarray ...
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bioRxiv - Molecular Biology 2020Quote: ... Particles were quantified by serial dilution methods by detection of plaques in HEK293Ad (Agilent) cells ...
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bioRxiv - Immunology 2020Quote: ... Signal detection of PXN was performed using polyclonal goat anti-rabbit AP (Dako D0487) and liquid permanent red (Dako K0640) ...
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bioRxiv - Microbiology 2022Quote: Peak detection and integration were done in Agilent G1701EA MSD Productivity ChemStation (Agilent Technologies) using the ChemStation Integrator and the following integration parameters to increase detection sensitivity ...
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bioRxiv - Plant Biology 2022Quote: ... Array signal detection/scanning was done using the Agilent dual laser microarray scanner (Agilent Technologies DNA Microarray Scanner ...
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bioRxiv - Microbiology 2023Quote: ... The sections were immunostained using the Dako EnVision FLEX + detection system (DAKO, Carpinteria, CA). The Dako PT link was used for deparaffinization and heat-induced epitope retrieval using Dako Target Retrieval Solution ...
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bioRxiv - Plant Biology 2024Quote: ... Metabolite detection and annotation were performed by Quant software (Agilent, Santa Clara, CA, USA), according to an accurate mass library of known markers generated by our group and run in the same system ...
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bioRxiv - Molecular Biology 2019Quote: ... RT-qPCR analyses were performed using Brilliant III Ultra-Fast SYBR® Green (Agilent) after DNase treatment using Turbo DNase (AMBION) ...
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bioRxiv - Bioengineering 2021Quote: ... were applied for 30 mins at RT and samples were exposed DAB substrate (Dako) for 5 mins ...
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bioRxiv - Neuroscience 2020Quote: ... then 2h at RT with a rabbit IgG anti-S100β antibody (1:250; Dako-Agilent ...
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bioRxiv - Cell Biology 2020Quote: ... RT-qPCR assays were analyzed with 2(-ΔΔCt) method 85 via MxPro software (Stratagene) and expressed as relative quantity to normalizer 86.
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bioRxiv - Genetics 2022Quote: ... cDNA was synthesized from 150 ng of total RNA with AffinityScript RT buffer (Agilent) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... at RT for 1hr and stained with primary antibodies diluted in Antibody Diluent (Dako) overnight at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... quantitative reverse transcriptase PCR (qRT-PCR) was performed with the Brilliant III Ultra Fast SYBR® Green QRT-PCR Mastermix (Agilent, Waldbronn, Germany): 5 µl Master Mix (supplied) ...
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bioRxiv - Microbiology 2021Quote: ... The PCR reaction was performed on a Stratagene MX3005p quantitative PCR instrument (Stratagene, USA). Results were expressed as Log10 FMDV RNA copies/108 copies 18S.
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bioRxiv - Molecular Biology 2020Quote: ... Samples were purified with the QIAquick PCR Purification Kit and the final purified library was validated on a TapeStation (Agilent, G2991AA) and sequenced in 300 bp pair-end reads on an Illumina MiSeq sequencer at the Genomic Facility at the University of Guelph (Guelph ...
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bioRxiv - Immunology 2021Quote: ... The amplified DNA purified and subsequently mutagenized by error-prone PCR (ePCR) via the GeneMorph II Random Mutagenesis Kit (Agilent Technologies) with a target nucleotide mutation frequency of 0-4.5 mutations/kb ...
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bioRxiv - Microbiology 2021Quote: ... (labeled regions A and B) were generated using the primer sets and cycle conditions indicated in Table S1B (EasyA PCR kit (Agilent Tech) and BioRad T100 thermal cycler) ...
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bioRxiv - Microbiology 2020Quote: ... Quantitative real-time PCR analysis of gene expression was undertaken using the KAPA SYBR FAST qPCR Kit and Stratagene Mx3005P qPCR System (Agilent Technologies). Thermal cycling conditions were as follows ...
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bioRxiv - Microbiology 2019Quote: ... A site directed mutagenesis of the cloned UBP-1 PCR product in the TOPO 2.1 vector was carried out using a QuikChange® multi-site directed mutagenesis kit (Agilent technologies) using the following primer combinations ...
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bioRxiv - Microbiology 2020Quote: ... we carried out site direct mutagenesis of pCR-ompT-GFP according to the Quikchange II site-directed mutagenesis kit (Agilent Technologies).
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bioRxiv - Cancer Biology 2022Quote: ... All cell lines were tested for mycoplasma at the beginning of the studies (tested negative with MycoSensor PCR Assay Kit, Agilent, #302109) and have never exhibited contamination symptoms after initial testing.
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bioRxiv - Immunology 2020Quote: ... The recombinant Ad5 genomes with HA inserts were linearized with PacI and buffer exchanged using a Strataprep PCR purification kit (Agilent Technologies). The linearized recombinant gDNA was transfected into 293 cells using the PolyFect Transfection Reagent (Qiagen) ...
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bioRxiv - Pathology 2019Quote: ... Pathogenic mutations and variations in the INF2 sequence were introduced by a PCR-based mutagenesis (Cat No: 200523, QuickChange II Site-Directed Mutagenesis kit, Agilent Technologies). To achieve stable expression of fragments in human podocytes ...
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bioRxiv - Cell Biology 2019Quote: ... hFN10 containing a TAG stop codon at position 1493 was generated by PCR-based mutagenesis with the Quick-change kit (Agilent Technologies), cloned into bacterial expression plasmid pET11a and verified by DNA sequencing ...
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bioRxiv - Synthetic Biology 2020Quote: ... The error-prone library of the initial hit of the ACE2 helix scaffolded design was constructed by error-prone PCR with a GeneMorph II Random Mutagenesis Kit (Agilent Technologies) with manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... The VDAC1 mutations at serine 215 to glutamate (S215E) was generated by PCR with QuikChange site-directed mutagenesis kit (Agilent Technology) using pET-VDAC1 as cDNA templates and the following primers ...
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bioRxiv - Genomics 2021Quote: ... RT qPCR analysis was run for the samples using a miScript SYBR Green PCR Kit (Cat. No. 218073) on a Stratagene Mx3005P (Agilent Technologies). Housekeeping gene GAPDH primers were purchased from Qiagen (Cat ...
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bioRxiv - Neuroscience 2020Quote: ... Human α7-nAChR R208I-GFP and E211N-GFP were generated from pcDNA3.1-CHRNA7-mGFP and human α3-nAChR I284R-GFP and N287E-GFP were generated from human α3-nAChR-GFP by PCR based QuikChange® Site-Directed Mutagenesis Kit (Agilent) according to the manufacturer’s protocol ...