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Citations for Agilent :
701 - 750 of 808 citations for C terminal Proghrelin Isoform Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The Seahorse XFp extracellular flux cartridge was hydrated with sterile water and incubated overnight at 30 °C and Seahorse XF calibrant solution (Agilent Technologies). After this ...
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bioRxiv - Bioengineering 2023Quote: ... The electrode temperature was controlled by submerging in 70 °C PBS solution with a P4010 thermal meter (Dostmann electronic, Germany) for temperature monitoring and a 34401A digit multimeter (Agilent, USA) for electrical resistance measurement ...
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bioRxiv - Immunology 2024Quote: ... Epitope retrieval was then performed at 95 °C for 10 min at pH 9 with Dako Target Retrieval Solution (Agilent, USA), in a Lab Vision PT Module (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: A total of 10 µg/ml rHA were coated on an ELISA plate at 4 °C ON and afterwards incubated with rabbit anti-HA antibody (1:2000 dilution) (Dako, A0001) 2h at RT ...
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bioRxiv - Biophysics 2023Quote: ... Cuvettes were then inserted into a preheated (50°C) Cary 3500 UV−Vis spectrophotometer controlled by an Agilent multizone Peltier temperature controller (Agilent Technologies). The samples were cooled to 1°C at a rate of 1°C/min while measuring the absorbance at 600 nm.
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bioRxiv - Physiology 2024Quote: Fuel preference of CRISPR/Cas9 Pparγ edited cells (γY4KO) and mock-edited cells (C) was measured using Seahorse Mito-Fuel Flex test (Agilent Technologies), in assay medium as above and according to the manufacturer protocol ...
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bioRxiv - Biophysics 2024Quote: In vitro NMR experiments were carried out at 25 °C on an 800 MHz Agilent spectrometer equipped with a cryogenic probe (Agilent Technologies). The buffer used in all NMR titration assay was 50 mM sodium phosphate buffer (pH 6.5 ...
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bioRxiv - Microbiology 2024Quote: ... Cultures were grown at 30 °C in 96-well plates in an BioTek Epoch 2 shaking incubator (Agilent, Santa Clara, CA) for 72 hours.
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bioRxiv - Microbiology 2024Quote: ... The plate was sealed with a gas permeable seal (Azenta Life Sciences 4ti-0516/96*) and incubated at 37°C and 237 cpm for indicated times in a Synergy H1 plate reader (Agilent BioTek). OD600nm was measured every 10 min ...
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bioRxiv - Cell Biology 2024Quote: hAC were fixed with 4% formalin and blocked for 1 h at 37°C (Protein-Block serum free, X0909 Dako Agilent). COL2 antibody (MA5-13026 ...
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bioRxiv - Synthetic Biology 2024Quote: ... Hydrolyzed amino acids were separated using ultra performance liquid chromatography (UPLC, Acquity - Waters, USA) on a C-8 column (Zorbax Eclipse XBD - Agilent, USA) at a flow rate of 0.6 mL/min and eluted off the column using a hydrophobicity gradient ...
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bioRxiv - Microbiology 2021Quote: ... The test tubes were incubated for 18-24 hours at 37 °C after which turbidity was observed and optical density recorded using “Cary Series UV/Vis” (Agilent Technologies, Germany). The lowest concentration that completely inhibited the growth of the test organisms as observed by the no growth when the concentration with the least optical density is plated out on nutrient agar was reported as the MIC ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and the consumption of NADPH was followed at 30 °C and 340 nm (Δε=6.22 mM-1 cm-1) with a Cary 60 UV-Vis spectrophotometer (Agilent Technologies, Germany).
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bioRxiv - Evolutionary Biology 2020Quote: ... The reaction was started with different concentrations of 3-butenoic acid and the consumption of NADPH was followed at 30 °C and 340 nm (Δε=6.22mM-1 cm-1) with a Cary 60 UV-Vis spectrophotometer (Agilent Technologies, Germany).
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bioRxiv - Evolutionary Biology 2020Quote: ... and stored at −80°C after verifying concentration and quality by spectrophotometer (Nanodrop, Thermo scientifics) and a Bioanalyzer (RNA 6000 Nano Kit, Agilent Technologies Inc). We produced libraries for these 21 individuals using the TruSeq RNA Library Prep Kit v2 (Illumina ...
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bioRxiv - Developmental Biology 2021Quote: ... The mutated forms of the amplified sequence in which the potential m6A sites were replaced by cytidine (C) or guanine (G) were constructed using the QuikChange II XL Site-directed Mutagenesis Kit (Agilent Technologies, 200518). The fragments were separately inserted into the pCambia2300 vector ...
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bioRxiv - Neuroscience 2019Quote: ... Paraffin embedded human brain sections were de-waxed for antigen retrieval with 90% formic acid (5 min) followed by citrate boiling (45min, 98°C DAKO Citrate Buffer, DAKO PT Link). Sections were then blocked using 0.1% (w/v ...
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bioRxiv - Cancer Biology 2020Quote: ... and cRNA was hybridized with ArrayXS Human (Oaklabs, Germany) at 65 °C for 17 hours using the Agilent Gene Expression Hybridization Kit (Agilent Technologies, USA), washed once with Agilent Gene Expression Wash Buffer 1 for one minute at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... blocked using 10% Normal Goat Serum (NGS) and incubated overnight with the following primary antibodies in 5% NGS overnight at 4°C: rabbit anti-GFAP (DAKO; #Z0334;1:1000), rat anti-L1-CAM (Millipore ...
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bioRxiv - Microbiology 2019Quote: ... The carbon isotope composition of CO2 was determined using gas chromatography combustion isotope ratio mass spectrometry (GC/C-IRMS) consisting of a gas chromatograph (7890A Series, Agilent Technology, USA) coupled via Conflo IV interface (ThermoFinnigan ...
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bioRxiv - Plant Biology 2021Quote: ... at 37⁰C (1:50 protein/trypsin ratio) and cleaned up with a C18 column (OMIX C18, Agilent, Santa Clara, CA, USA). Nano-liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS ...
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bioRxiv - Cancer Biology 2020Quote: ... slides with PCa tissue samples (n=144) were incubated at 60°C for 2 hours followed by target retrieval in a PT Link instrument (Agilent DAKO, PT200) using EnVision FLEX Target Retrieval Solution ...
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bioRxiv - Molecular Biology 2022Quote: ... they were first permeabilized using an acetone freeze step for 7 min at -20° C before staining with guinea-pig anti-insulin (Dako, Carpinteria, CA), Alexa Fluor 488 or 647 goat anti-guinea-pig (Thermo Fisher Scientific ...
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bioRxiv - Pathology 2020Quote: ... Deparaffinization and antigen retrieval were performed in Target Retrieval Solution with pH 9 (ACE2) or pH 6 (Ki-67) at 97°C for 20 min using the PT Link platform (Dako, Glostrup, Denmark). Subsequently ...
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bioRxiv - Physiology 2019Quote: ... for 15 minutes and then incubated overnight at 4°C with primary antibodies diluted in DAKO antibody diluent: anti-Insulin (DAKO, 1:1000), anti-BRD4 (Bethyl laboratories ...
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bioRxiv - Cancer Biology 2019Quote: ... for 4 hours at 4°C with rotation and processed using the Agilent Bravo liquid handling system (Agilent Technologies, Santa Clara, CA). Beads were washed twice with 0.1% NP-40 in Tris-buffered saline (50 mM Tris-HCl with 150mM NaCl ...
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bioRxiv - Molecular Biology 2019Quote: ... and ORAI1 (c.319G>A; p.V107M) point mutations were introduced by site-directed mutagenesis using the Pfu DNA polymerase (Stratagene, La Jolla, USA).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... column maintained at ambient temperature (30ΰC) coupled to a quadrupole time-of-flight (Q-TOF) mass spectrometer (Q-TOF-LC/MS 6510 classic G6150A, Agilent technologies, USA) with a dual ESI as ion source ...
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bioRxiv - Microbiology 2021Quote: ... for 1 hour at room temperature or overnight at 4°C using the alkaline phosphatase-anti-alkaline-phosphatase (APAAP) technique according to the manufacturer’s protocol (Dako, Carpinteria, CA, USA). All antibodies were diluted in TBS with 0.1% BSA ...
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bioRxiv - Synthetic Biology 2019Quote: ... Injections of 5 µL of each sample were separated using a Zorbax Eclipse Plus C-18 column (2.1 × 100 mm, 1.8 µm) (Agilent, Santa Clara, CA, USA) at a flow rate of 0.35 mL/min ...
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bioRxiv - Developmental Biology 2021Quote: ... Tissue sections were then incubated overnight at 4°C with primary antibodies (Extended Table 2) in Antibody Diluent (Agilent Dako, S080983-2). Following a wash step ...
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bioRxiv - Developmental Biology 2021Quote: ... Tissue sections were then incubated overnight at 4°C with primary antibodies (Extended Table 2) in Antibody Diluent (Agilent Dako, S080983-2). Following a wash step ...
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bioRxiv - Developmental Biology 2022Quote: ... The PVDF membranes were blocked in 5 % nonfat dry milk diluted in Tris-buffered saline with 0.05 % Tween-20 for 1 h at room temperature and then incubated overnight at 4 °C with antibodies against FN (1:70000, rabbit anti-fibronectin, DAKO, Hamburg, Germany). After washing with TBS-T ...
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bioRxiv - Immunology 2022Quote: ... and the ATP production rate were quantified at 37°C using the Agilent Seahorse XF Real-Time ATP Rate Assay Kit (Agilent, 103592-100) with the Seahorse XFe96 analyzer ...
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bioRxiv - Cancer Biology 2022Quote: ... was carried out by drying silica NPs at 37°C for 48 hours and finally placing the dry sample onto a Cary 630 FTIR spectrometer (Agilent Technologies Ltd) for the measurement of transmittance spectra ...
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bioRxiv - Biophysics 2022Quote: Fluorescence titrations were carried out at 25 °C using 1 × 0.3 cm path-length cuvettes in a Cary Eclipse spectrofluorimeter (Agilent Technologies, Waldbronn, Germany). Experiments were carried out in 20 mM K-phosphate ...
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bioRxiv - Microbiology 2023Quote: ... Fluorescence was measured at 50°C over 1 h at excitation 360 nm emission 460 nm on a BioTek Synergy HTX Multimode Reader (BioTek, Agilent, Tx, USA). The reaction rate was calculated as the maximum velocity over 12 sequential readings ...
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Retinal affectation in Huntington’s disease mouse models concurs with a local innate immune responsebioRxiv - Neuroscience 2023Quote: ... and incubated overnight in a humid chamber at 4°C with rabbit anti-glial fibrillary acidic protein (GFAP) antibody (1:500, Z0334, DAKO, Agilent, Glostrup, Denmark), rabbit anti-ionized calcium binding adaptor molecule 1 (Iba-1 ...
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bioRxiv - Molecular Biology 2023Quote: ... seedlings were exposed on the plates to 8 kJ/m2 UV-C light in a Stratalinker 2400 (Stratagene, La Jolla, California, US) and transferred back into growth chamber for 5 h ...
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bioRxiv - Cancer Biology 2023Quote: ... Tumor xenograft slides were incubated at 60 °C for 2 h followed by antigen retrieval in a PT Link instrument (Agilent DAKO, PT200) using EnVision FLEX Target Retrieval Solution ...
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bioRxiv - Physiology 2023Quote: ... slides (4 μm thickness) were baked at 60° C for 45 min and treated with a low pH target retrieval solution (#DM829; Dako, Glostrup, Denmark) in a DAKO PT Link HIER machine (PTlink 200 ...
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bioRxiv - Genomics 2023Quote: ... and the size and concentration of the finished PCHi-C libraries was assessed by high-sensitivity DNA ScreenTape Analysis (Agilent #5067-5584) on an Agilent 2200 Tapestation.
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bioRxiv - Microbiology 2020Quote: ... The desorbed VOCs were subsequently collected on a cold trap at −10°C and introduced into a GC-QTOF (model Agilent 7890B GC and the Agilent 7200AB QTOF, USA) by heating the cold trap for 10 min to 280°C ...
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bioRxiv - Developmental Biology 2020Quote: ... sections were permeabilized with 0.25% TritonX-100 in PBS and autoclaved (121°C, 10 min) with Target Retrieval Solution (Agilent Technologies, Santa Clara, California). Sections were incubated with Blocking One Histo (Nacalai Tesque Inc. ...
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bioRxiv - Microbiology 2021Quote: ... Supernatant was harvested by centrifugation at 1500 rcf for 4 minutes at 23°C and filtered using a Captiva 96 well 0.2 μm filter plate (Agilent Technologies, Santa Clara, CA) prior to titer measurement by RP-UHPLC.
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bioRxiv - Physiology 2021Quote: Oxygen consumption was measured at 37°C from isolated mouse and human islets after overnight recovery using a Seahorse XF24 analyzer (Agilent, Santa Clara, CA). Islets were seeded at a density of 75 islets/well ...
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bioRxiv - Microbiology 2020Quote: ... the epitopes were unmasked by heating at 95°C for 30 min prior to detection on a Dako OMNIS™ automate (Agilent Technologies, USA). When indicated ...
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bioRxiv - Biochemistry 2019Quote: ... and chromatographic separation was performed at +40 °C using a Pursuit pentafluorophenyl (PFP) column (150 x 2.1 mm, 3.5 µm; Agilent technologies, Santa Clara, CA, USA) connected to a guard column (Pursuit PFP) ...
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bioRxiv - Biophysics 2021Quote: ... incubated for 16 h at 4 °C and purified by HPLC on a Zorbax C3 column with dimensions of 21 × 150 mm (Agilent, Santa Clara, CA) using a gradient of 20-90 % acetonitrile in water with 0.1 % trifluoroacetic acid (TFA ...
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bioRxiv - Cell Biology 2022Quote: Analysis of mitochondrial functions in HAP 1 WT and HAP 1 Δ(c+δ) cells was performed on Seahorse XFe24 (Agilent Technologies, USA) as described previously (Nichols et al. ...