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Citations for Agilent :
501 - 550 of 808 citations for C terminal Proghrelin Isoform Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2019Quote: ... Sections were immersed in EDTA-NaOH buffer (0.37 g/L EDTA, pH 9.0, preheated to 65°C) in Dako PT LINK (Dako; #PT101) and heated to 97°C for 20 minutes ...
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bioRxiv - Cancer Biology 2019Quote: ... For antigen retrieval the slides were incubated for 30 min at 98 °C in 1x Target retrieval solution (Dako). Sections were stained by adding primary antibodies diluted in immunofluorescence buffer (IFB ...
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bioRxiv - Neuroscience 2020Quote: ... with 5% BSA and 0.4% triton X-100) followed by incubation overnight at 4°C with 1:500 rabbit anti-C3 antibody (Dako) and 1:10,000 guinea pig anti-vGluT2 (Synaptic Systems ...
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bioRxiv - Immunology 2020Quote: ... Seahorse sensor cartridges were incubated at 37°C in a non-CO2 incubator overnight with Seahorse XF Calibrant pH 7.4 (Agilent) to rehydrate the sensor probes ...
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bioRxiv - Physiology 2021Quote: ... Samples were incubated overnight at 4°C in primary antibodies targeting anti-insulin (1:200, Abcam #Ab7872, Dako #A0564), anti-glucagon (1:100 ...
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bioRxiv - Microbiology 2020Quote: ... sections underwent antigen retrieval in citrate buffer (pH 6.0, 20 min at 98°C) followed by blocking of endogenous peroxidase (peroxidase block, S2023, Dako) for 10 min at room temperature (RT) ...
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bioRxiv - Immunology 2021Quote: ... for 10 minutes at 37°C for F4/80 or blocked with a protein block (catalog number x0909, Dako) for 10 minutes followed by an Fc block (catalog number 553142 ...
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bioRxiv - Bioengineering 2022Quote: ... retinal cross-sections were treated with antigen retrieval solution at 99 °C for 1 h (PH 6.1; Dako #S1699) and washed in 1x PBS ...
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bioRxiv - Systems Biology 2020Quote: ... Samples were injected directly onto a column packed in-house (75 μm inner diameter) with 3.5 μm Zorbax C-18 (Agilent) and analyzed using a 90 min gradient from 5%-30% solvent B (Solvent A ...
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bioRxiv - Systems Biology 2020Quote: ... Samples were injected directly onto a column packed in-house (75 μm inner diameter) with 3.5 μm Zorbax C-18 (Agilent) and analyzed using a 90 min gradient from 5%-30% solvent B (Solvent A ...
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bioRxiv - Molecular Biology 2022Quote: ... The enzyme was heat inactivated at 75°C for 2 minutes and then removed with 1μl of Strataclean resin (Agilent). Capped RNAs were precipitated using 2M ammonium acetate and isopropanol to remove any residual nucleotides ...
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bioRxiv - Biochemistry 2019Quote: ... All final extracts were dried under vacuum (Extractor Plus®, Agilent; settings: 30°C, 2 h, 1000 rpm, VAQ).
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bioRxiv - Cell Biology 2019Quote: ... 0.1 mM IPTG and appropriate antibiotics at 16 °C for 20 h using BL21 CodonPlus (DE3) RIL (Agilent Technologies). Siz1 (167-508 ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... A transfer line set at 250 °C led to a 5977A mass spectrometer (Agilent Technologies Inc., Santa Clara, CA) with a solvent delay of 5 min ...
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bioRxiv - Pathology 2021Quote: ... The region containing random fragments of the VWF plasmid was made double stranded using primers P9/P10 (annealing temperature = 65.0°C) and Herculase II (Agilent) with 16-20 rounds of PCR amplification (Figure S1B) ...
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bioRxiv - Molecular Biology 2021Quote: ... Oxygen consumption was measured at 37°C using an XF24 extracellular analyzer (Seahorse Bioscience Inc., North Billerica, MA, USA) [19].
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bioRxiv - Genetics 2019Quote: ... and AFF4 NM_014423.4:c.772C>T mutations were engineered using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... and incubating it at 95 °C for 23 min using a pt link pretreatment system (Dako, Agilent, Glostrup, Denmark). We removed the staining container at room temperature and allowed the slides to cool for 20 min ...
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bioRxiv - Microbiology 2023Quote: ... and incubating it at 95 °C for 23 min using a pt link pretreatment system (Dako, Agilent, Glostrup, Denmark). We removed the staining container at room temperature and allowed the slides to cool for 20 min ...
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bioRxiv - Plant Biology 2023Quote: ... A gradient elution system was optimized with the two mobile solvents to separate metabolites on an Eclipse XDB-C18 analytical column (250 × 4.6 mm, 5 µM, 25 ◦C, Agilent). The gradient solvent system was composed of ratios of solvent A to B ...
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bioRxiv - Developmental Biology 2023Quote: ... Antigen retrieval was conducted using Dako Citrate buffer (pH 6.0) for 30 minutes at 98°C in a PT Link rinse station (Dako). Tissue sections were blocked in PBTx containing 5% BSA (Merck ...
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bioRxiv - Microbiology 2023Quote: ... ammonia and separated using high pH reverse phase Zorbax 300 extend C-18 4.6 mm x 250 mm (Agilent) column and liquid chromatography AKTA Micro system (GE Healthcare ...
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bioRxiv - Synthetic Biology 2023Quote: ... The cultivation was carried out at 30 °C in constant mixing on BioTek Synergy H1 Multi-Mode Reader (Agilent Technologies Inc. ...
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bioRxiv - Biophysics 2023Quote: ... The PCR product was digested overnight with DpnI at 37 °C and transformed into XL1-Blue Supercompetent cells (Agilent) and the mutation was confirmed by sequencing (Eurofins Genomics) ...
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bioRxiv - Cell Biology 2023Quote: ... The plate was incubated at 30°C with continuous double orbital shaking in a BioTek Epoch2 microplate reader (Agilent), and the OD600 was measured every 10 minutes for 24 hours ...
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bioRxiv - Microbiology 2023Quote: ... 12µl of sample was incubated with 2µl of 25µM AR2 primer at 72°C for 2min using 10x AffinityScript RT buffer and enzyme (Agilent Technologies), 0.1M DTT ...
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bioRxiv - Microbiology 2024Quote: ... samples were cleared for 7 min at 14,000 × g at 4 °C and transferred to LC-MS polypropylene conical vials (Agilent). Vials were stored at −80 °C until analysis using LC-MS.
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bioRxiv - Cell Biology 2024Quote: ... Cells were incubated overnight at 4°C with primary antibodies in DAKO REALTM Anti-body diluent (Agilent, Cat#S2022). Secondary antibodies (1:500 dilution ...
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bioRxiv - Plant Biology 2024Quote: 21-day-old in vitro grown arabidopsis plants were exposed to 3,000 J/m2 of UV-C using the Stratalinker 2400 (Stratagene). Plant material was harvested prior irradiation for control (0 ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated overnight at 4 °C with the primary antibody: GFAP (Agilent/Dako, z033420-2, dilution 1:750) or MOG (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated overnight at 4 °C with the primary antibody: GFAP (Agilent/Dako, z033420-2, dilution 1:750) or MOG (Sigma-Aldrich ...
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bioRxiv - Genetics 2024Quote: ... Cryosections were incubated overnight at 4°C with primary antibodies: polyclonal anti-GFAP (Dako, Agilent, Santa Clara, California, USA) (1:5000 in 1% BSA ...
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bioRxiv - Genetics 2024Quote: ... Cryosections were incubated overnight at 4°C with primary antibodies: polyclonal anti-GFAP (Dako, Agilent, Santa Clara, California, USA) (1:5000 in 1% BSA ...
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bioRxiv - Cancer Biology 2021Quote: ... were incubated with the indicated primary antibodies (Reagents) overnight at 4 °C followed by Envision/diaminobenzidine detection (Dako, Glostrup, Denmark) and hematoxylin counterstaining/mounting (Entellan ...
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bioRxiv - Cancer Biology 2021Quote: ... The plate was incubated in a CO2-free 37°C incubator for 1 hour and a Mitochondrial Stress Test (Agilent) was performed according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: Immunohistochemical staining of formalin-fixed, paraffin-embedded xenografts was performed after antigen retrieval (120°C, 7 min at pH9) and peroxidase blocking (Dako) using the UltraVision LP detection system (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... and the cDNA hybridised to an 8 Chamber Agilent slide at 65°C for 16 hours before washing the slide with Oligo aCGH Wash Buffer 1 (Agilent) for 5 minutes at room temperature and Oligo aCGH Wash Buffer 2 (Agilent ...
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bioRxiv - Molecular Biology 2021Quote: ... Thermal stability at a constant 37°C was measured by a thermal shift assay using a Mx3000p real-time PCR instrument (Agilent technologies ...
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bioRxiv - Molecular Biology 2020Quote: ... were induced with 2μg/mL doxycycline for 6 hours prior to crosslinking at 100mJ/cm2 at 4°C in a Stratalinker 1800 (Stratagene). Cells were then processed according to the eCLIP protocol for input and immunoprecipitated samples until cDNA was obtained ...
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bioRxiv - Molecular Biology 2021Quote: ... Forty reaction cycles of 10 s at 95 and 30 s at 58 °C were carried out on a Multiplex 3005 Plus (Stratagene/Agilent). The amplicon coordinates relative to the 47S rRNA initiation site (BK000964v3 ...
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bioRxiv - Systems Biology 2020Quote: ... 30 °C) and the quantity of ethylene in the headspace was determined by gas chromatography (7820A GC system, Agilent Technologies). The nitrogenase activity is expressed as % conversion of added acetylene into ethylene ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... at 4 °C overnight and immunostained using the streptavidin-biotin peroxidase technique (Envision universal peroxidase kit; Dako Cytomation, Milan, Italy). After incubation ...
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bioRxiv - Physiology 2020Quote: ... followed by a final elongation step at 72 °C for 5 min (SureCycler 8000, Agilent technologies, Santa Clara, CA, USA). In order to amplify different transcripts via nested PCR from the renin gene ...
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bioRxiv - Immunology 2019Quote: ... incubated overnight at 4 °C with polyclonal guinea pig anti-insulin antibody (1:100 in 1% BSA in DPBS, DAKO) followed by 1 h room temperature incubation either with Alexa Fluor® 488 AffiniPure donkey anti-guinea pig or with DyLight™ 594 AffiniPure donkey anti-guinea pig secondary antibody (1:100 in 1% BSA in DPBS ...
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bioRxiv - Cancer Biology 2020Quote: ... France) Citrate buffer pH6 was used for antigen retrieval 20min à 96°C (Target Retrieval so-lution low pH, Dako) and DAB and Hematoxylin staining were revealed using ImPath detection kit (DAB OB Sensitive Detection Kit ...
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bioRxiv - Biochemistry 2019Quote: Dried peptides were reconstituted in 15 μl of 0.1% formic acid in water and 5 μl of sample was applied to an ACQUITY UPLC C18 reverse phase column (Waters) maintained at 40°C using an Infinity 1290 autosampler and HPLC (Agilent). Peptides were eluted at 0.1 ml/min using a varying mobile phase gradient from 95% phase A (FA/H2O 0.1/99.9 ...
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bioRxiv - Immunology 2019Quote: ... The density of HLA-C per bead was calculated using QIFIKIT calibration beads according to the manufacturer’s instructions (Dako, K0078). Calibration beads contained five populations of beads conjugated to different numbers of mouse immunoglobulin ...
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bioRxiv - Microbiology 2020Quote: ... Hydrolyzed amino acids were separated using ultra performance liquid chromatography (UPLC, Acquity, Waters) on a C-8 column (Zorbax Eclipse XBD, Agilent) at a flow rate of 0.6 mL/min ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred to a nitrocellulose membrane before probing using a primary anti IL-36γ antibody (Biotechne (R&D) UK) overnight at 4°C and a secondary goat antibody (Dako) for 1 hour at room temperature to visualise any protein cleavage.
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bioRxiv - Bioengineering 2020Quote: ... rehydrated slides were subject to antigen retrieval using citric acid buffer at 120°C and 20 psi in a pressure cooker (DAKO). Afterwards ...