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Citations for Agilent :
451 - 500 of 808 citations for C terminal Proghrelin Isoform Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... NR2F2 expression vector containing the NM_021005.4:c.737G>A (p.Arg246His) variant was generated by site-directed mutagenesis (QuikChange II, Stratagene), using the forward primer ...
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bioRxiv - Cell Biology 2022Quote: ... The Pfn1 c.318_321delTGCC mutation was introduced in the targeting vector by PCR-mediated mutagenesis (QuikChange Lightning, Agilent). Targeting of the construct was done into the E14Tg2a embryonal stem cell line ...
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bioRxiv - Genomics 2019Quote: ... The following day microarrays were scanned using an Agilent C-scanner with Feature Extraction 9.1 software (Agilent technologies). RT datasets are available in the Gene Expression Omnibus (GEO ...
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bioRxiv - Neuroscience 2019Quote: ... Incubation with the primary antibody was done overnight at 4°C and with secondary antibodies (DAKO #P0217, #P0260) for 1.5 hours at RT with washing in TBS-Tween trice for 15 minutes each after each incubation ...
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bioRxiv - Microbiology 2019Quote: ... The extract was dissolved in methanol and was subjected to preparative HPLC-MS with C-18 column (21.2 mm × 250 mm, 7.0 µm, Agilent) using an acetonitrile/water gradient (0.1% formic acid ...
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bioRxiv - Biochemistry 2019Quote: ... The supernatant was analyzed on a Poroshell 120 EC-C18 column (2.7 μm, 3.0 × 50 mm, 45 °C, Agilent) using a linear gradient from 5 to 55% acetonitrile over 6.5 min with 0.1% formic acid as the aqueous mobile phase after an initial hold at 95% 0.1% formic acid for 0.5 min (0.6 mL/min ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated with primary antibodies at 4°C overnight: mouse monoclonal anti-CD31 (1:100, JC70A, Dako), rabbit polyclonal anti-VWF (1:100 ...
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bioRxiv - Biochemistry 2021Quote: ... The cleaned mixtures were applied to a C-18 reverse phase column equipped on an HPLC (Agilent 1260) with a gradient of 0.1 M KH2PO4 (pH 6.0 ...
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bioRxiv - Systems Biology 2021Quote: ... for 20 min at 96°C with the reagents EnVision FLEX Target Retrieval Solution High pH (50x) (Dako) for CAT and EnVision FLEX Target Retrieval Solution Low pH (50x ...
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bioRxiv - Plant Biology 2021Quote: ... Samples were derivatized at 70°C and injected in a splitless mode to a GC-MS system (Agilent 7890A chromatograph ...
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bioRxiv - Cell Biology 2022Quote: ... the cartridge sensors were incubated overnight at 37 °C in a hydration/ calibration solution (XF Calibrant; Agilent Technologies). Specially designed polystyrene tissue culture-treated 96-well microplates with a clear flat bottom (Seahorse XF96 V3 PS Cell Culture Microplates ...
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bioRxiv - Bioengineering 2022Quote: ... then incubated separately for 30 min at 37 °C with secondary Ab (anti-mouse ab HRP conjugated, DAKO Envision K4001 or anti-rabbit ab HRP conjugated ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were incubated overnight at 4°C with the following primary antibodies: mouse anti-BrdU (1:100, Dako), rat anti-BrdU (1:100 Oxford Biotech) ...
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bioRxiv - Biochemistry 2022Quote: ... a ZORBAX Extended C-18 column (1.8 μm, 50 mm × 4.6 mm; Agilent Technologies, Santa Clara, CA, USA) was used ...
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bioRxiv - Neuroscience 2019Quote: ... Mutation of IMPA1-C and IMPA-1S poly(A) sites was performed by PCR site-directed mutagenesis (Agilent) of Firefly-IMPA1-L vector ...
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bioRxiv - Synthetic Biology 2020Quote: ... Exponential phase culture consists of stationary culture diluted 1:20 in Lb then incubated at 37°C until an OD600 of ∼0.4-0.8 is reached (as determined using an Agilent Cary 60 UV-Vis Spectrometer using manufacturer documentation) ...
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bioRxiv - Cancer Biology 2019Quote: ... Slides were incubated at 4°C overnight and then washed and visualized with DAB+ substrate chromogen (Agilent Technologies). The patient groups with 249T-P positive and negative were divided at 10% cutoff value.
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bioRxiv - Microbiology 2021Quote: ... After deparaffinization, antigens were activated (121°C, 10 min) with Target Retrieval Solution pH6.0 (Dako Cytomation, Glostrup, Denmark), and endogenous HRP was inactivated by hydroperoxide treatment ...
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bioRxiv - Biochemistry 2021Quote: ... The samples were loaded onto a Zorbax SB-300 C-18 column (4.6 * 250 mm, 5 µm) (Agilent Technologies ...
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bioRxiv - Biochemistry 2021Quote: NIH 3T3 cells were transiently transfected with pFA2-cJunWT (PathDetect c-Jun trans-Reporting System from Agilent Technologies), or the indicated pFA2-cJun mutants or pFC2-dbd (empty vector) ...
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bioRxiv - Genetics 2021Quote: ... 1.7 μm particle size) maintained at 65°C was coupled to an Agilent 1290 Infinity UHPLC (Agilent Technologies) for ESI positive and negative modes ...
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bioRxiv - Developmental Biology 2021Quote: ... then at 4°C overnight with primary antibodies diluted in Dako REAL Antibody Diluent (Agilent, Santa Clara, CA). We used the following primary antibodies ...
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bioRxiv - Cancer Biology 2020Quote: Patient-derived explant tissue sections were stained for cytokeratin (DAKO, Cat. M3515; 1:100 overnight at 4°C), α-smooth muscle actin (αSMA ...
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bioRxiv - Biochemistry 2022Quote: ... Assay of NADH:quinone oxidoreductase activity was conducted at 37 °C with a BioTek Synergy Neo2 plate reader (Agilent) in buffer composed of 10 mM MES pH 6.0 ...
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bioRxiv - Biochemistry 2022Quote: Intrinsic fluorescence quenching experiments were performed at 25 °C with a Varian Cary Eclipse Fluorescence Spectrophotometer (Agilent Technologies) with slits set at 5 nm bandwidth ...
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bioRxiv - Microbiology 2022Quote: ... Chlamydia antigen retrieval consisted of pressure cooking (98°C) in citrate buffer (Dako/Agilent, Santa Clara, CA, USA) for 20 min ...
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bioRxiv - Synthetic Biology 2023Quote: ... Exponential phase culture consists of stationary culture diluted 1:20 in Lb then incubated at 37°C until an OD600 of ∼0.4-0.8 is reached (as determined using an Agilent Cary 60 UV-Vis Spectrometer using manufacturer documentation) ...
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bioRxiv - Microbiology 2022Quote: ... Chlamydia antigen retrieval consisted of pressure cooking (98°C) in citrate buffer (Dako/Agilent, Santa Clara, CA, USA) for 20 min ...
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bioRxiv - Immunology 2022Quote: ... C antibody (clone W6/32) that was immobilized and covalently linked to Protein A cartridges (Agilent G5496-60000). Peptides were acid eluted from antibody bound ProteinA cartridges using 0.1M acetic acid/0.1%TFA followed by desalting with C18 solid-phase extraction (SPE) ...
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bioRxiv - Neuroscience 2023Quote: Reactions were monitored by liquid chromatography-mass spectrometry (LC-MS) using C-18 column (4.6 × 50 mm, 1.8 μm, Agilent) with a linear gradient (water/MeCN 5%/95% → MeCN 100% ...
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bioRxiv - Neuroscience 2023Quote: ... The XF96 plate was then transferred to a temperature-controlled (37 °C) Seahorse extracellular flux analyzer (Agilent Technologies) and allowed a temperature equilibration period before assay cycles (consisting of 1-min mix ...
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bioRxiv - Cancer Biology 2023Quote: Quality and quantification of the Hi-C libraries was assessed using the 2100 Bioanalyzer (Agilent, DNA 7500 kit). An equimolar pool of the different samples was sequenced on the Illumina NextSeq 550 System in a 75bp paired-end setup ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were then incubated overnight at 4 °C with appropriate dilution of anti-Ki67 (1:100, Dako, M7240) in TBS containing Horse Serum (2%) ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were deparaffinized by incubation at 60°C for 10 min and incubated with PT-Link (Dako, Agilent) for 20 min at 95°C in low pH to detect VDR ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were deparaffinized by incubation at 60°C for 10 min and incubated with PT-Link (Dako, Agilent) for 20 min at 95°C in low pH to detect VDR ...
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bioRxiv - Cancer Biology 2022Quote: ... in a citrate buffer for 20 min at 95°C for TP53 (1:800, Dako, M7001 DO-7), BCL-10 (1:1000 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... was heated to 40°C for an isocratic 6-minute run paired with a 5977B GC/MSD (Agilent).
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bioRxiv - Microbiology 2023Quote: ... The cultures were incubated at 37 °C by shaking for 24 h in a BioTek Epoch 2.0 (Agilent). The quantification of bacteria and phages was done by counts of colony-forming units (CFU/mL ...
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bioRxiv - Biochemistry 2023Quote: ... HPLC was performed on an Agilent 1260 infinity II, equipped with a C-18 column (Mackerey-Nagel, 5 µm, ref 760.100.40) using acetonitrile (HPLC grade, Agilent) and ddH2O (0.1% (v/v ...
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bioRxiv - Genomics 2023Quote: ... Size and concentration of the finished Hi-C libraries was assessed by DNA ScreenTape Analysis (Agilent #5067-5582) on an Agilent 2200 Tapestation.
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bioRxiv - Genomics 2023Quote: Quality and quantification of the Hi-C libraries was assessed using the 2100 Bioanalyzer (Agilent, DNA 7500 kit). Four biological replicates have been pooled in an equimolar manner and subjected to sequencing using the Illumina NextSeq 550 System in a 75bp paired-end setup ...
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bioRxiv - Cancer Biology 2024Quote: ... Antigen retrieval was then performed at 97 °C for 10 minutes with Target Retrieval Solution (Agilent, S236784-2) on a PT Module (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... The primary incubation was performed overnight at 4°C (phospho-Ser129, BioLegend Cat # 825701, 1:2000; GFAP, DAKO Cat # GA524 ...
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bioRxiv - Biochemistry 2024Quote: ... the cartridge sensors were incubated overnight at 37 °C in a hydration/ calibration solution (XF Calibrant; Agilent Technologies). Specially designed polystyrene tissue culture-treated 96-well microplates with a clear flat bottom (Seahorse XF96 V3 PS Cell Culture Microplates ...
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bioRxiv - Molecular Biology 2019Quote: ... The mixture was pre-incubated at 37°C for 5 min and AffinityScript Multiple Temperature Reverse Transcriptase (Agilent Technologies) was added to each toeprinting reaction at a final content of 10.2% (v/v ...
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bioRxiv - Genetics 2021Quote: ... followed by incubation with 525 ml of assay medium at 37°C in an incubator without CO2 (Agilent Technologies) for 1 h ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were then incubated for 1 hour at 37°C in atmospheric CO2 in pH 7.4 unbuffered RPMI1640 with 1.67 mM glucose (Seahorse bioscience). Islets were first stimulated with high glucose (16.7 mM ...
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bioRxiv - Genomics 2020Quote: ... Hybridisations were carried out over 17 hours at 65° C at 10rpm rotation and washed following manufacturer’s instructions (Agilent). Datasets are available at ArrayExpress ...
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bioRxiv - Cell Biology 2019Quote: ... Protein lysates were incubated at 4°C with a mouse monoclonal antibody directed against the FLAG-tag (M2, Stratagene). Immunocomplexes were pulled down by incubation with protein G sepharose ...
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bioRxiv - Cancer Biology 2019Quote: ... The concentration and size distribution of Hi-C library DNA after PCR amplification was determined by tapestation (Agilent Technologies), and the Hi-C libraries were sequenced on Illumina Hi-Seq 2500 or Illumina Hi-seq 4000 at 50 cycles.