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Citations for Agilent :
401 - 450 of 3510 citations for 7 8 Dihydro 6H 1 6naphthyridin 5 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: The monkeys were scanned on an actively shielded 7-Tesla 68-cm horizontal bore scanner with a DirectDrive console (Agilent, Santa Clara, California) with a Siemens AC84 gradient subsystem (Erlangen ...
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bioRxiv - Cell Biology 2020Quote: ... and Nup133-mEGFP(-7) were cloned from the repair template plasmids constructed above via the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent, Santa Clara, CA). For each CRISPR cell line ...
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bioRxiv - Cancer Biology 2021Quote: ... before low pH antigen retrieval and staining with CD8a and Ki67 (Supplementary Table 7) antibodies and secondary antibody (EnVision+ HRP-rabbit, Dako, catalog #K400311-2) using the EnVision DuoFlex system (Dako) ...
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bioRxiv - Cancer Biology 2023Quote: ... using the clone DO-7 monoclonal antibody (GA61661-2, Dako Omnis, ready-to-use, incubation 20 min, Agilent Technologies, Santa Clara, CA, USA). Antibody detection was performed using EnVision FLEX/HRP (GV80011-2 ...
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bioRxiv - Molecular Biology 2023Quote: ... Quality of the RNA samples (RIN score >7) was verified on the Agilent 2100 Bioanalyzer using the RNA 6000 kit (Agilent, cat# 5067-1513). 1 µg of each RNA sample was then used for poly(A ...
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bioRxiv - Cell Biology 2020Quote: ... Cyanine-3 (Cy3) labeled cRNA was prepared from 200 ng of total RNA using the One-Color Quick Amp Labeling kit (Agilent Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... One μg/ml GFP antibody (Nacali Tesque Inc., cat# GF200) and 0.5 μg/ml HRP-conjugated mouse antibody (Dako, cat# P0447) were used for detecting DNA-binding proteins ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Purified total RNA (100 ng) was labelled with Cyanine3 (Cy3) using a Low Input Quick Amp WT labelling kit (One-color; Agilent Technologies). The Cy3-labelled cRNA was checked for its amount (> 5 μg ...
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bioRxiv - Molecular Biology 2021Quote: ... dilution 1:100)] in TNB blocking buffer for overnight at 4°C followed by one-hour incubation with secondary antibodies: EnVisionTM+ System-HRP (DAKO K4002). TRITC-based Tyramide reagent pack from Perkin Elmer was used to amplify the fluorescence of Ki67 and c-Kit ...
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bioRxiv - Physiology 2021Quote: ... Cyanine-3 (Cy3) labeled cRNA was prepared from 200 ng of total RNA using the One-Color Quick Amp Labeling kit (Agilent Technologies), according to the manufacturer's instructions ...
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bioRxiv - Plant Biology 2021Quote: ... The L480P mutation in GL2 was generated by one-step PCR-based site-directed mutagenesis (Scott et al., 2002) using PfuUltra II Fusion HS DNA polymerase (Agilent Technologies) with primers listed in Supplemental Table 5 ...
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bioRxiv - Microbiology 2020Quote: ... RNA was processed and labeled for microarray analysis using the One-Color Microarray-Based Gene Expression Analysis Low Input Quick Amp WT Labeling kit (Agilent Technologies), according to the manufacturer’s instructions.
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bioRxiv - Systems Biology 2020Quote: ... Double-stranded complementary DNA (cDNA) synthesis and array processing were performed by One-Color Microarray-Based gene Expression Analysis (Agilent Technologies). The images were analysed with the software DEVA (v1.2.1) ...
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bioRxiv - Microbiology 2021Quote: ... One ul of each of the RNA-seq libraries was loaded on a Bioanalyzer 2100 DNA High Sensitivity chip (Agilent Technologies) to check for size and absence of primer dimers ...
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bioRxiv - Plant Biology 2022Quote: ... then washed at room temperature using the Agilent One-Color Microarray-Based Gene Expression Analysis protocol (Agilent Technology, V 6.5, 2010). The hybridized array was immediately scanned with an Agilent Microarray Scanner D (Agilent Technologies ...
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bioRxiv - Plant Biology 2022Quote: ... and gl2Amo mutants were generated by one-step PCR-based site-directed mutagenesis (Scott et al., 2002) using PfuUltra II Fusion HS DNA polymerase (Agilent Technologies). The Phusion® Site-Directed Mutagenesis Kit (Thermofisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... One final wash with PBS was performed before mounting the coverslips on glass slides using DAKO mounting medium (DAKO Cytomation, S3023) or acquiring images in PBS when using Ibidi slides.
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bioRxiv - Microbiology 2020Quote: ... Gene-level intensities were scaled based on the intensity values of ten different in vitro-synthesized transcripts contained in the One Color RNA Spike-In kit (Agilent Technologies) in order to account for technical variation associated with sample processing ...
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bioRxiv - Microbiology 2022Quote: ... Samples were washed three times with PBS and one time with distilled water and mounted with Dako Fluorescent Mounting Medium (Dako, Denmark). Samples were visualized in the epifluorescence microscope Olympus BX53 fluorescence microscopy with UPLFLN 100× oil objective (numerical aperture 1.30) ...
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bioRxiv - Genetics 2023Quote: ... Cyanine-3 (Cy3)-labeled cRNAs were prepared with 100ng of total RNA using a One-Color Low Input Quick Amp Labeling kit (Agilent Technologies). The specific activities and cRNA yields were determined by using a NanoDrop ND-1000 (Thermo Fisher Scientific) ...
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bioRxiv - Physiology 2023Quote: ... Cyanine-3 (Cy3) labeled cRNA was prepared from 200 ng of total RNA using the One-Color Quick Amp Labeling kit (Agilent Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Zoology 2023Quote: ... Total RNA was quantified using a NanoDropTM One spectrophotometer and quality assessed with an Agilent 2100 Bioanalyser using an RNA chip (Agilent Technologies). For library construction all RNA samples had a RIN value >7 ...
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bioRxiv - Molecular Biology 2020Quote: ... Final library yields and size were measured on a Bioanalyzer 2100 using high sensitivity reagents (Agilent Technologies, Supplementary Figure 8).
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bioRxiv - Cancer Biology 2021Quote: ... Endogenous peroxidases were blocked with peroxidase inhibitor (CM1) for 8 minutes before incubating the section with CD3 antibody (Dako, #A0452) at 1:300 dilution for 60 minutes at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... Agilent gene expression microarray 60K slide (Design ID: 72363. SurePrint G3 Human Gene Expression 8 × 60K Microarray Kit, Agilent Technologies) were used ...
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bioRxiv - Genomics 2019Quote: ... Whole genome expression data was obtained using Agilent Sureprint G3 Mouse Gene Expression 8×60K microarrays (Agilent Technologies cat.# G4852B) with Low Input Quick Amp Labeling protocol (Agilent Technologies cat.# 5190-2331 ...
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bioRxiv - Molecular Biology 2019Quote: ... Sample input was 900ng total RNA (determined by Qubit RNA HS reagents, Thermo) with a RIN >8 (TapeStation High Sensitivity RNA ScreenTape Assay, Agilent). RNA was fragmented for 15 minutes and libraries were constructed with a 1/5th dilution of NEB adaptor and 12 cycles of PCR amplification with dual-indexing primers ...
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bioRxiv - Genetics 2019Quote: ... UV Crosslinker, CL-1000, 254 nm, 100 V, 8 W [UVP/Analytik Jena] or Stratalinker UV crosslinker Model 1800 [Stratagene]). Under these conditions ...
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bioRxiv - Systems Biology 2019Quote: ... An amount of 100 ng of total RNA was hybridised with Cy-3 and subsequently hybridised on microarrays (SurePrint G3 8×60K, Agilent). The raw fluorescence signals were scanned with DNA Microarray Scanner SureSelect (Agilent ...
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bioRxiv - Microbiology 2020Quote: ... Hydrolyzed amino acids were separated using ultra performance liquid chromatography (UPLC, Acquity, Waters) on a C-8 column (Zorbax Eclipse XBD, Agilent) at a flow rate of 0.6 mL/min ...
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bioRxiv - Cell Biology 2021Quote: ... RNA samples were labeled and hybridized to a SurePrint G3 Human GEO microarray 8 × 60K Ver 3.0 (Agilent, CA, USA), and the raw data were normalized using the 75-percentile shift ...
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bioRxiv - Systems Biology 2021Quote: ... respectively) on a 300 × 7.7 mm PL Hi-Plex H+ (8 µm particle size) column (Agilent Technologies, Santa Clara, CA) and 4 x 3 mm ID Carbo-H guard (Phenomenex ...
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bioRxiv - Cell Biology 2022Quote: ... The Cy3-labeled DNA was hybridized overnight to 8×60K 60mer oligonucleotide spotted microarray slides (Agilent Technologies, design ID 074809). For each protocol ...
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bioRxiv - Cell Biology 2022Quote: ... The Cy3-labeled DNA was hybridized overnight to 8×60K 60mer oligonucleotide spotted microarray slides (Agilent Technologies, design ID 028005). For experiments where samples yielded <50 ng/μl of RNA ...
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bioRxiv - Neuroscience 2022Quote: ... A frequency of 59.673 MHz and an amplitude of 8 dBm was generated by a signal generator (EXG Analog Signal Generator, NS171B, 9 kHz – 3 GHz, Agilent) and fed into the transmitter and reception boards of the scanner via a respective custom made power splitter.5
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bioRxiv - Genomics 2022Quote: ... The library was amplified using 8 PCR cycles and verified on a Fragment Analyzer using the HS NGS fragment kit (Agilent). The library was quantified by qPCR using the KAPA Library quantification kit (Roche ...
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Local assembly of long reads enables phylogenomics of transposable elements in a polyploid cell linebioRxiv - Genomics 2022Quote: ... Purified DNA was precipitated by addition of 8 mL of ethanol and resuspended in TE buffer and size was analyzed by TapeStation (Agilent) prior to library preparation ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... A 20 µl sample was injected into the Hi-Plex H column (300×7.7 mm; 8 µm particle size, Agilent Tech.). 0.1% formic acid in Milli-Q water (Merck Millipore ...
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bioRxiv - Cancer Biology 2020Quote: Microarray was performed on in vitro cultures of dMMR and CIN MC38 CRC cells using the SurePrint G3 Mouse Gene Expression v2 Microarray (8×60K) (Agilent) system ...
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bioRxiv - Microbiology 2022Quote: The bacterial transcriptome was interrogated using a custom SurePrint G3 GE 8×60K chip designed in house and produced by Agilent Technologies (4) ...
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bioRxiv - Cancer Biology 2019Quote: ... For cDNA expression microarray analysis using SurePrint G3 Human GE 8×60K Ver.2.0 (Agilent Technologies, Santa Clara, CA, USA), total RNA was sent to a commercial service (Hokkaido System Science ...
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bioRxiv - Immunology 2020Quote: ... RNA sample quality control assessment (RNA integrity number ≥8) was performed with the RNA Pico 6000 chip on Bioanalyzer 2100 (Agilent) and were quantified with Qubit Fluorometer (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2019Quote: ... Tissue sections were incubated with 4% bovine serum albumin (BSA) and 8% serum in 1x wash buffer ‘en vision’ (Dako) to eliminate non-specific protein binding sites ...
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bioRxiv - Developmental Biology 2021Quote: Microarray analysis was performed on six control and six IL1B samples (O4+ cells isolated at P5 after in vivo PBS or IL1B treatment) using Agilent Whole Mouse Genome Oligo Microarrays 8×60K (Agilent). Raw data are available in Dataset S2 ...
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bioRxiv - Systems Biology 2020Quote: ... The resulting Cy3-labeled cRNA was hybridized along with a uniform Cy5-labeled reference sample (as an internal standard) to custom gene-specific 8 x 60k oligonucleotide Agilent arrays (Agilent design ID ...
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bioRxiv - Cell Biology 2019Quote: ... and RNA quality was determined using a Bioanalyzer 2100 (Agilent; RIN>8, OD 260/280 and OD 260/230>1.8). RNA libraries were prepared using the TruSeq Stranded mRNA Library Preparation Kit (Illumina ...
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bioRxiv - Cell Biology 2022Quote: ... cells in a concentration of 2×105 cells/well were transferred into an 8-well plate (Agilent Seahorse XFp miniplate), which was previously coated overnight with poly-L-lysine (Santa Cruz Biotechnology ...
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bioRxiv - Developmental Biology 2022Quote: ... Nucleosomal DNA libraries were pooled in groups of 8 per lane and quantified with by TapeStation (Agilent 2200 TapeStation system) run using a D1000 ScreenTape (Agilent) ...
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bioRxiv - Neuroscience 2023Quote: ... cells were seeded at 2.5 x 104 cells/well into 8-well Seahorse XFp cell culture miniplates (Agilent Technologies, USA) and treated with the sialyltransferase inhibitor for 48 hours ...
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bioRxiv - Synthetic Biology 2022Quote: ... 10 µl of protein sample in assay buffer (20 mM sodium phosphate, 20 mM sodium chloride, pH 8) was injected onto a C4 column (Agilent) and eluted in a water (1% FA ...