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Citations for Agilent :
401 - 450 of 997 citations for Prestained Protein Peptide Standards since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... using the Protein G AssayMAP Bravo (Agilent) system ...
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bioRxiv - Molecular Biology 2019Quote: ... onto a peptide trap (Zorbax 300 SB-C18, 0.3 i.d. × 5 mm, 5 µm, 300 Å; Agilent Technologies, Santa Clara, CA, USA) for concentration and desalting with a pump running in isocratic mode with 0.1% formic acid in water ...
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bioRxiv - Biochemistry 2020Quote: ... Protein or peptide (fragments of peptide) intact mass was acquired using an MSD-ToF electrospray ionisation orthogonal time-of-flight mass spectrometer (Agilent Technologies Inc.). Data analysis was performed using MassHunter Qualitative Analysis Version B.07.00 (Agilent ...
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bioRxiv - Systems Biology 2020Quote: 200 ng samples of peptides were separated in a high performance liquid chromatography system (1260 Infinity LC System, Agilent, Santa Clara, CA) and a C18 column (3.5 μm particle size ...
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bioRxiv - Microbiology 2020Quote: Two different glycosidases were used for specific removal of N-glycans: peptide N-glycosidase F (PNGase F) and endo N-glycosidase H (Endo H) (Prozyme, Hayward, CA). PNGase F removes all N-glycans from gp120/41 glycoprotein whereas Endo H removes high-mannose and hybrid glycans ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The pooled TMT-labeled peptide sample was fractionated using basic pH reverse-phase liquid chromatography with an Agilent 1260 Infinity HPLC system (Agilent, CA, USA). The chromatography conditions were as follows ...
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bioRxiv - Bioengineering 2019Quote: ... followed by overnight staining with 1:300 dilutions of rat-anti-C-peptide (DSHB; GN-ID4-S) and mouse-anti-CD31 (Dako; M082329-2) primary antibodies ...
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bioRxiv - Synthetic Biology 2019Quote: Peptides were analyzed using an Agilent 1290 liquid chromatography system coupled to an Agilent 6460QQQ mass spectrometer (Agilent Technologies, Santa Clara, CA) operating in MRM mode ...
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bioRxiv - Immunology 2021Quote: ... Peaks were identified based on known glycans from the Glyko InstantAB biantennary and high-mannose partitioned library standards (Prozyme, Hayward, CA, USA), with the total percent of each glycoform reported as the integrated area under the identified peak divided by the sum of the AUC (area under the curve ...
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bioRxiv - Biophysics 2020Quote: ... Calibration curves of individual and mixed amino acids were prepared using either 250 pmol stocks of corresponding individual amino acids or a 250 pmol amino acid standard mix (Agilent #5061-3331). Quantification was performed using calibration curves of the respective amino acid standards.
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bioRxiv - Developmental Biology 2021Quote: ... Deletion and mutation variants were generated using standard cloning techniques and the QuickChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies, 200521).
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bioRxiv - Cell Biology 2022Quote: ... All other constructs were made by PCR amplification followed by standard restriction enzyme cloning or by site-directed mutagenesis kit (QuickChange II, Agilent Technologies, 200523). All oligonucleotides were obtained from Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... Concentrated extracts were spiked with the recovery standard (BFR-SCS, Wellington Laboratories) and analyzed by HRGC/HRMS (Agilent 6890/Thermo MAT95 XL) using electron impact ionization (EI) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... the tissue was soaked in 200μl dichloromethane containing 200ng 2-tetradecyl acetate (internal standard) in 2ml glass vials with PTFE-coated caps (Agilent, Santa Clara, USA) for one hour ...
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bioRxiv - Neuroscience 2022Quote: ... and the sample supernatant was collected and dried under nitrogen along with labeled internal standards before sylilation and processing on an Agilent 5973 quadrupole GC-MS (Agilent, Wilmington, DE). The ratios of the ions m/z 354/358 (dopamine retention time (RT) ...
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bioRxiv - Systems Biology 2023Quote: ... and confirmed the quality of the RNA samples using a Fragment Analyzer (Advanced Analytical Technologies, Inc.) with the Standard Sensitivity RNA Analysis Kit (Agilent, #DNF-471).
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bioRxiv - Biochemistry 2023Quote: ... sulfur and phosphorus contents were determined by inductively coupled plasma mass spectrometry (ICP-MS/MS) on an Agilent 8800 Triple Quadropole ICP-MS instrument by comparison to an environmental calibration standard (Agilent 5183-4688), a sulfur (Inorganic Ventures CGS1 ...
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bioRxiv - Developmental Biology 2024Quote: ... Universal Reference RNA was used as an internal standard to enable reliable comparison of relative mRNA levels in multiple samples (Stratagene Cat# 740000) [101] ...
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bioRxiv - Biophysics 2021Quote: hnRNPA1* (where * denotes that the hexa-peptide 259-264 is deleted) protein and deletion constructs were expressed as N-terminally tagged hSUMO fusion proteins in BL21 (DE3) RIPL cells (Agilent) in LB media ...
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bioRxiv - Cancer Biology 2021Quote: ... and an additional serum-free protein block (DakoCytomation) to reduce nonspecific binding of endogenous proteins ...
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bioRxiv - Synthetic Biology 2019Quote: ... After blocking with serum-free protein block (Dako), they were incubated with the primary antibodies for 120 min at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-glial acidic fibrillary protein (GFAP)(Dako), mouse anti-glutamic acid decarboxylase 67 (GAD67 ...
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bioRxiv - Bioengineering 2020Quote: Proteins were expressed in BL21(DE3)Gold (Stratagene) and purified as described previously6 ...
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bioRxiv - Biophysics 2021Quote: ... proteins were expressed using BL21(DE3) cells (Agilent). The cells were grown at 37°C until an optical density at 600nm of .6 then induced with 0.2mM isopropyl-Beta-D-thiogalactoside overnight at 18° C ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein blocking was performed for 20 min (Dako). NUP210 primary antibody (Atlas ...
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bioRxiv - Biochemistry 2019Quote: ... Protein was overexpressed in BL21(DE3) RIL (Agilent) at 37 °C in Terrific Broth (TB ...
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bioRxiv - Pathology 2021Quote: ... Slides were placed in a Protein Block (DAKO) for 10 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... and Protein-Block solution (DAKO Agilent technologies, X0909) respectively ...
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bioRxiv - Cancer Biology 2020Quote: ... and Protein-Block solution (DAKO Agilent technologies, X0909) respectively ...
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bioRxiv - Cell Biology 2021Quote: ... blocked in Dako Protein Block (Cat #X0909, Agilent), and stained by secondary antibodies (1:1000 ...
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bioRxiv - Microbiology 2022Quote: ... The slides were blocked with protein Block (Agilent) to prevent nonspecific antibody binding ...
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bioRxiv - Microbiology 2023Quote: ... The slides were blocked with Protein Block (Agilent) to prevent nonspecific antibody binding ...
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bioRxiv - Cell Biology 2024Quote: ... Step 4 was protein blocking (Background Sniper, Dako Protein Block or Normal block ...
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bioRxiv - Neuroscience 2023Quote: ... sections were blocked using 10% protein block (Dako) in PBS containing 0.5% Triton-X-100 for 1 h at RT ...
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bioRxiv - Microbiology 2023Quote: ... then blocked with Dako protein block (Agilent Technologies) for 30 minutes ...
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bioRxiv - Bioengineering 2024Quote: ... followed by protein block (X0909, Dako North America). At room temperature ...
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bioRxiv - Synthetic Biology 2019Quote: ... Peptides prepared for shotgun proteomic experiments were analyzed by using an Agilent 6550 iFunnel Q-TOF mass spectrometer (Agilent Technologies, Santa Clara, CA) coupled to an Agilent 1290 UHPLC system as described previously [36] ...
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bioRxiv - Microbiology 2020Quote: ... Peptides were then analysed by liquid chromatography mass spectrometry (LC-MS) using an Agilent 1290 Infinity HPLC (Agilent Technologies, Santa Clara, CA, USA) connected to a Xevo G2-XS QTof mass spectrometer (Waters ...
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bioRxiv - Immunology 2022Quote: ... Quality was measured by capillary electrophoresis using the Fragment Analyzer and the ‘Total RNA Standard Sensitivity Assay’ (Agilent Technologies, Inc. Santa Clara, USA). All samples in this study showed high quality RNA Quality Numbers (RQN > 9.4) ...
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bioRxiv - Biochemistry 2019Quote: ... in 200μl of dichloromethane containing 200 ng of 2-tetradecyl acetate (internal standard) in 2ml glass vials with polytetrafluoroethylene-coated caps (Agilent, Santa Clara, California). After one hour ...
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bioRxiv - Biochemistry 2019Quote: ... The p-coumaric acid and caffeic acid peaks were identified by comparing the retention times to authentic standards and by mass spectrometry (Agilent G6120, quadrupole MS). The integrated peak areas were converted to concentrations in mM based on calibration curves generated with authentic standards.
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The transcription factor reservoir and chromatin landscape in activated plasmacytoid dendritic cellsbioRxiv - Molecular Biology 2021Quote: ... and quality measured by capillary electrophoresis using the Fragment Analyzer and the ‘Total RNA Standard Sensitivity Assay’ (Agilent Technologies, Inc. Santa Clara, USA). All samples in this study showed high RNA Quality Numbers (RQN ...
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bioRxiv - Immunology 2022Quote: ... and quality measured by capillary electrophoresis using the Fragment Analyzer and the ‘Total RNA Standard Sensitivity Assay’ (Agilent Technologies, Inc. Santa Clara, USA). All samples in this study showed high quality RNA Quality Numbers (RQN ...
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bioRxiv - Cell Biology 2023Quote: IHC followed a standard protocol using a PT link antigen retrieval system with high pH antigen retrieval solution (both Dako Pathology/Agilent Technologies, Stockport, UK). Antibody for pSTAT3 (1:100 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... External calibration of the mass spectra was performed using nano-electrospray generated ions from a commercially available calibration standard (Agilent, Santa Clara, CA). Native mass spectra were manually processed using MassLynx (Waters Corp. ...
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bioRxiv - Cell Biology 2022Quote: ... Digested peptides were separated using a 50 cm reversed-phase column packed in-house (Agilent Poroshell EC-C18, 2.7 µm, 50cm x 75 µm) and were eluted at a flow rate of 300 nl/min using a linear gradient with buffer A (0.1% FA ...
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bioRxiv - Cell Biology 2022Quote: ... Digested peptides were separated using a 50 cm reversed-phase column packed in-house (Agilent Poroshell EC-C18, 2.7 µm, 50cm x 75 µm). The peptides were eluted from the column at a flow rate of 300 nl/min using a linear gradient with buffer A (0.1% formic acid (FA) ...
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bioRxiv - Immunology 2020Quote: ... SARS-CoV-2 peptide-loaded HLA class I tetramers were generated by conjugation of biotinylated peptide-loaded HLA class I easYmers® with phycoerythrin (PE)-conjugated streptavidin (Agilent, Santa Clara, US) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Generated tryptic peptides were loaded onto a trap column (300SB-C18, 5 × 0.3 mm, 5-μm particle size; Agilent Technologies, Santa Clara, CA, USA) connected through a zero-dead-volume union to the self-packed analytical column (C18 ...
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bioRxiv - Cancer Biology 2023Quote: ... The reaction was quenched with TFA and the peptides were isolated and desalted using reverse phase C18 and SCX cartridges (Agilent technologies, Santa Clara, CA). Tryptic peptide mixtures were evaporated with speed vacuum and dissolved in 2 % acetonitrile solution acidified with 1 % TFA at a final concentration of 2 μg/uL.