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Citations for Agilent :
301 - 350 of 997 citations for Prestained Protein Peptide Standards since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... Protein Block Serum Free reagent (Dako). Primary antibody incubation was performed at 4°C overnight using the ideal dilution for each antibody (Supplementary Table 5) ...
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bioRxiv - Cell Biology 2021Quote: ... and incubated in Protein Block (Dako) for 5 min ...
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bioRxiv - Cancer Biology 2021Quote: ... and protein (Dako cat. no. X0909) blocking reagents for 5 minutes each ...
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bioRxiv - Microbiology 2023Quote: ... diluted with protein block buffer (Dako) for 2 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... protein blocker (Agilent Technology, X090930-2). Slides were incubated with a biotinylated anti-pimonidazole mouse IgG1 monoclonal antibody diluted 1:50 in Background Sniper (Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Dako Protein Block (Agilent Cat#X0909), ‘Normal’ block (Agilent Cat#S202386-2) ...
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bioRxiv - Systems Biology 2020Quote: ... lyophilized samples were resuspended in Buffer A (5 mM NH4HCO2/ 2% ACN) and 5 mg peptide material (5 mg/ml) was loaded onto a reversed phase column (ZORBAX 300Extend-C18, Agilent). Peptides were separate at a flow rate of 2 ml/min and a constant column temperature of 40 °C using a binary buffer system ...
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bioRxiv - Cell Biology 2021Quote: Ap-proximately 1000 ng of peptides from each biological replicate before SCX fractionation were first injected onto an Agilent 1290 Infinity UHPLC system (Agilent) on a 50-cm analytical column packed with C18 beads (Dr Maisch Reprosil C18 ...
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bioRxiv - Genomics 2022Quote: ... The total peptide concentration was measured by UV absorbance at 210 nm using an LC system with UV detector (Agilent), with roughly 10 mg tissue yielding 500-750 μg peptide mixture.
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bioRxiv - Bioengineering 2021Quote: Peptides used for pMHC refolding were synthesized in housed using the Liberty Blue Peptide Synthesizer (CEM) and validated using LC-MS (Agilent). To generate pMHC molecules for bioconjugation ...
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bioRxiv - Cell Biology 2019Quote: ... sample aliquots containing 200 µg of dried peptides were subjected to phosphopeptide enrichment using IMAC cartridges and a BRAVO AssayMAP liquid handling platform (Agilent) as recently described 47.
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bioRxiv - Plant Biology 2020Quote: ... The dried peptides were dissolved in 1% TFA solution to perform zip-tip cleanup using C-18 Omix tips (Agilent). Digests were dried in a speedvac as elution-2 (E2 ...
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bioRxiv - Molecular Biology 2020Quote: ... samples were passed through an immobilized pepsin column (2mm 2cm) at 200 μl min−1 and the digested peptides were captured on a 2mm 1cm C8 trap column (Agilent) and desalted ...
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bioRxiv - Biochemistry 2022Quote: Purity of the wild-type or mutant FAM237A peptides was analyzed by HPLC using an analytical C18 reverse-phase column (Zorbax 300SB-C18, 4.6 × 250 mm, Agilent Technologies). The FAM237A peptides were eluted from the C18 reverse-phase column by an alkaline acetonitrile gradient composed of alkaline solvent A and B [15] ...
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bioRxiv - Biochemistry 2022Quote: ... Peptide fractions were eluted from a semi-preparative C18 reverse-phase column (Zorbax 300SB-C18, 9.4 × 250 mm; Agilent Technologies) by an acetonitrile gradient ...
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bioRxiv - Molecular Biology 2022Quote: ... at 200 µl min-1 and the digested peptides were captured on a 2 mm x 1 cm C8 trap column (Agilent) and desalted ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The peptides were then purified and concentrated using OMIX Tips C18 reversed-phase resin (Agilent Technologies, Santa Clara, CA, USA). The peptides were dehydrated in a vacuum centrifuge and analysed by NanoLC-MS/MS ...
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bioRxiv - Immunology 2023Quote: Fluorophore-conjugated (or biotinylated) peptides were purified via reversed phase chromatography on a C18 column (Pursuit XRs C18 column, Agilent) to separate peptide-dye conjugates from unconjugated peptides or dyes ...
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bioRxiv - Plant Biology 2023Quote: ... 10 μg of TMT-labeled peptides were loaded onto 10 cm capillary columns packed with 5 μM Zorbax SB-C18 (Agilent), which was connected using a zero dead volume 1 μm filter (Upchurch ...
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bioRxiv - Systems Biology 2023Quote: ... and digested using a modified in-solution sample preparation protocol.94 One hundred micrograms of desalted peptides were fractioned on an microflow HPLC (1260, Agilent) using a XBridge peptide BEH C18 column (130 Å ...
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bioRxiv - Systems Biology 2023Quote: ... equivalent amounts of each digested sample were pooled before proceeding to a peptide fractionation using a manual workflow based on Stage-Tips or an automatic workflow with the AssayMAP Bravo (Agilent).
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bioRxiv - Cell Biology 2023Quote: ... the HA epitope sequence was inserted immediately 3’ to the signal peptide sequence by site directed mutagenesis using the QuickchangeXL site directed Mutagenesis kit (Agilent) to obtain the following intermediate vector ...
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bioRxiv - Plant Biology 2023Quote: ... and portions of samples corresponding to 5 µg of peptide were analyzed by nanoflow reverse-phase liquid chromatography-mass spectrometry using a 15 cm C18 Zorbax column (Agilent), a Dionex Ultimate 3000 RSLC nano-UPLC system ...
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bioRxiv - Bioengineering 2023Quote: ... Peptides were loaded to a nanoscale HPLC column composed of 10 cm of Polaris C18 5 μm packing material (Agilent), followed by 4 cm of Partisphere 5 SCX (Whatman) ...
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bioRxiv - Microbiology 2023Quote: ... Solid phase extraction of peptides was done using equilibrated C18 pipette tips (Bond Elut OMIX 100 µl pipette tips, Agilent). The samples are aspirated for 10 cycles for maximum binding efficiency and then eluted in LC-MS/MS vials with 100 µl of 0.1% TFA in water:acetonitrile (at a ratio of 30:70 (v/v)) ...
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bioRxiv - Molecular Biology 2024Quote: ... quenched samples were passed through a co-immobilized pepsin/fungal XIII protease column (1mm × 2cm) at 50 µl min−1 and the digested peptides were captured on a 1mm × 1cm C8 trap column (Agilent) and desalted ...
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bioRxiv - Cancer Biology 2024Quote: ... enrichment of phosphorylated peptides was performed on the Bravo automated liquid handling platform using AssayMAP Fe(III)-NTA cartridges (Agilent). In brief ...
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bioRxiv - Cell Biology 2024Quote: ... pcDNA3.1zeo+/ nFlag-cmScarlet-hRobo1 was generated by inserting the Flag tag sequence at the N-terminus after the signal peptide sequence of Robo1 with QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies ...
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bioRxiv - Cell Biology 2020Quote: Mito fuel flex test: Agilent Seahorse XF Mito Fuel Flex Test Kit was used as per the standard protocol by Agilent Seahorse (https://www.agilent.com/cs/library/usermanuals/public/XF_Mito_Fuel_Flex_Test_Kit_User_Guide%20old.pdf) ...
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bioRxiv - Biochemistry 2022Quote: ... The H335A mutation that renders PLC-γ1 catalytically inactive was introduced into the transfer vector encoding wild-type PLC-γ1 (residues 21-1215) or PLC-γ1 (D1165H) using standard primer-mediated mutagenesis (Agilent Technologies ...
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bioRxiv - Plant Biology 2020Quote: Quantitative RT-PCR quantification was conducted using the standard curve method as described in the Methods and Applications Guide from Agilent Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... Metabolites were identified and areas integrated based on GC retention time and mass spectra as compared with authentic standards and in conjunction with MSD ChemStation Data Analysis Application (Agilent) using in-house and Wiley metabolite libraries ...
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bioRxiv - Molecular Biology 2022Quote: ... 450 ng of digested RNA was spiked with 50 ng of internal standard (13C stable isotope-labeled nucleosides from S. cerevisiae) and analyzed by LC-MS (Agilent 1260 Infinity system in combination with an Agilent 6470 Triple Quadrupole mass spectrometer equipped with an electrospray ion source (ESI)) ...
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bioRxiv - Biochemistry 2022Quote: ... The APPI-Q-TOF MS instrument was calibrated in the mass range of 50-3000 m/z using an internal calibration standard (low concentration tuning mix solution) supplied by Agilent Technologies ...
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bioRxiv - Cancer Biology 2022Quote: ... paraffin-embedded tissue sections were dewaxed and stained according to the H/E standard protocol using a CoverStainer (Dako, Agilent).
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bioRxiv - Cancer Biology 2022Quote: ... paraffin-embedded tissue sections were dewaxed and stained according to the H/E standard protocol using a CoverStainer (Dako, Agilent).
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bioRxiv - Neuroscience 2021Quote: ... Both Southern blot probes were generated by standard PCR and subjected to random labelling using a Prime-It II Random Primer Labelling Kit (#300385, Agilent) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: ... The resulting Cy3-labeled cRNA was hybridized along with a uniform Cy5-labeled reference sample (as an internal standard) to custom gene-specific 8 x 60k oligonucleotide Agilent arrays (Agilent design ID ...
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bioRxiv - Neuroscience 2021Quote: ... 25 and 50 ppb with Sc-45 (ICP-MS internal standard mix 1 ug/mL in 2% HNO3, Agilent Technologies) as the internal standard for Cu-63.
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bioRxiv - Biochemistry 2020Quote: ... Mass signal intensities corresponding to the product (TAFTIPSI) and internal standard (Ac-YTAFTIPSI) were measured on a Rapidfire autosampler (Agilent) equipped with a C18 solid phase cartridge ...
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bioRxiv - Immunology 2020Quote: Extracts and 4-OHE1 standard were analyzed using a liquid chromatography system (LC; 1200 series, Agilent Technologies, Santa Clara, CA) that was equipped with a reversed-phase analytical column (length ...
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bioRxiv - Microbiology 2021Quote: ... and concentration of each IVT standard were confirmed using the Agilent Bioanalyzer RNA 6000 Nano assay (Agilent, Santa Clara, CA) prior to dilution in nuclease-free water to working concentrations.
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bioRxiv - Immunology 2020Quote: ... Measurement of RNA concentration and quality control was performed using a 2100 Bioanalyzer instrument with the standard manufacturers protocol (Agilent).
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bioRxiv - Molecular Biology 2022Quote: ... The supernatants and NAD+ standards were analysed by Electrospray Ionization mass spectrometry (ESI-MS) using an integrated HPLC/ESI-MS system (1290 Infinity, Agilent Technologies/Triple Quadrupole 6410 ...
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bioRxiv - Cancer Biology 2022Quote: ... Low concentration Tuning Mix standard (G1969-85000) was used to calibrate the mass spectrometry and ion mobility spectrometry and obtained from Agilent Technologies (Santa Clara ...
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bioRxiv - Genomics 2022Quote: ... Libraries were quantified with Qubit dsDNA HS Assay kit and visualized with Standard Sensitivity NGS Fragment Analysis Kit (Advanced Analytical DNF-473) and Fragment Analyzer 5200 (Agilent). Libraries were sequenced using Illumina NovaSeq flowcell with 100 bp single-end runs.
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bioRxiv - Microbiology 2022Quote: ... One hundred μL of the extract was mixed with 10μL of 4-chlorobenzotrichloride as injection standard and analyzed by GC (HP 7890 Series GC, Agilent, USA) with a 20 m 0.18mm (18μm film thickness ...
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bioRxiv - Plant Biology 2022Quote: ... Quantitative RT-PCR quantification was conducted using the standard curve method as described in the Methods and Applications Guide from Agilent Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... and input libraries were analysed using Bioanalyzer DNA 1000 or Fragment Analyzer Standard Sensitivity (ref: 5067-1504 or ref: DNF-473, Agilent) to estimate the quantity and validate the size distribution ...
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bioRxiv - Genomics 2023Quote: ... Libraries were quantified with Qubit dsDNA HS Assay kit and visualized with Standard Sensitivity NGS Fragment Analysis Kit (Advanced Analytical DNF-473) and Fragment Analyzer 5200 (Agilent). Libraries were sequenced using Illumina NovaSeq flow cell with 100bp single-end runs.