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Citations for Agilent :
251 - 300 of 997 citations for Prestained Protein Peptide Standards since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... The scanned images were analyzed using the standard procedures described in the Agilent Feature Extraction software 9.5.3.1 (Agilent Technologies).
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bioRxiv - Microbiology 2020Quote: ... Mutations were introduced using standard PCR or using the QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent, 210515). Double A3H fusion constructs I-I ...
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bioRxiv - Microbiology 2021Quote: ... for correct size and purity with the NGS standard sensitivity fragment analysis kit (Agilent Cat. No. DF-473). Of all suitable samples a pool of 2 nM was generated and loaded to the Illumina MySeq 2500 system.
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bioRxiv - Microbiology 2021Quote: ... and DNF-473 Standard Sensitivity NGS Fragment Analysis Kit (1 bp −6000 bp; Agilent, Santa Clara, CA, USA). Average library length was 341 bp ...
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bioRxiv - Physiology 2022Quote: ... paraffin-embedded tissue sections were dewaxed and stained with Hematoxylin eosin standard staining using a CoverStainer (Dako - Agilent). An Iron Stain Kit was used to identify iron pigments (AR15811-2 ...
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bioRxiv - Physiology 2022Quote: ... paraffin-embedded tissue sections were dewaxed and stained with Hematoxylin eosin standard staining using a CoverStainer (Dako - Agilent). An Iron Stain Kit was used to identify iron pigments (AR15811-2 ...
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bioRxiv - Biophysics 2023Quote: ... Site-directed mutagenesis was performed using standard two-step PCR mutagenesis using the Herculase II DNA Polymerase (Agilent), with sequences verified by Sanger sequencing ...
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bioRxiv - Biochemistry 2023Quote: ... SUMO-TP_KpEnc mutants were constructed via standard QuikChange Site-Directed mutagenesis as developed by Stratagene (La Jolla, CA) per standard protocol ...
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Mitochondrial Apolipoprotein MIC26 is a metabolic rheostat regulating central cellular fuel pathwaysbioRxiv - Cell Biology 2023Quote: ... and quality measured by capillary electrophoresis using the Fragment Analyzer and the ’Total RNA Standard Sensitivity Assay’ (Agilent Technologies ...
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bioRxiv - Biochemistry 2020Quote: ... Peptides were ionized by electrospray ionization and the ions were introduced into a 6510 Q-TOF mass spectrometer (Agilent). Spectra were analyzed with MassHunter Workstation Qualitative Analysis software version B.06.00 (Agilent).
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bioRxiv - Biochemistry 2021Quote: ... The peptide separation was performed on a C18 reverse phase column (Agilent, Poroshell 120, 0.3×35 mm, 2.7 μL) with a linear gradient of 8-48% B over 30 min (A ...
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bioRxiv - Microbiology 2019Quote: ... 1% acetonitrile/0.5% formic acid was used as eluent for 5 minutes to trap and desalt the peptides on the enrichment column (Zorbax SB C18, 0.3 × 5 mm, Agilent). An acetonitrile/0.1% formic acid gradient from 5% to 40% acetonitrile was then used within 120 minutes to separate the peptides on a Zorbax 300 SB C18 ...
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bioRxiv - Immunology 2022Quote: ... and the resulting peptides were captured and desalted by C-8 trap column (2.1 × 20 mm, Zorbax Eclipse XDB-C8 trap (Agilent) followed by loading on to C-18 column (2.1 × 50 mm in size ...
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bioRxiv - Biochemistry 2020Quote: ... The peptide solutions were desalted using OMIX C18-micro solid phase extraction (SPE) columns (Agilent, Santa Clara, CA, USA). A Q-Exactive mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... alkylated eluents were digested with trypsin at 37°C and the extracted peptides were then cleaned with ZipTips (Agilent) and dried in a SpeedVac (VWR) ...
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bioRxiv - Plant Biology 2019Quote: ... 25 μg of peptides were loaded unto 20 cm capillary columns packed with 5 μM Zorbax SB-C18 (Agilent), which was connected using a zero dead volume 1 μm filter (Upchurch ...
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bioRxiv - Synthetic Biology 2020Quote: Mass spectrometry analysis of tryptic peptides was conducted on a 6460 Triple Quad LC-MS system (Agilent Technologies, USA). From the samples prepared according to the protocol described above ...
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bioRxiv - Microbiology 2020Quote: ... Solid phase extraction of peptides was performed using C18 reversed phase sorbent containing 100 µL pipette tips according to the manufacturer’s instructions (Agilent, Santa Clara ...
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bioRxiv - Biophysics 2019Quote: ... samples were passed through an immobilized pepsin column (2mm × 2cm) at 50μl min-1 and the digested peptides were captured on a 2mm × 1cm C8 trap column (Agilent) and desalted ...
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bioRxiv - Biochemistry 2020Quote: ... TFA) and fractionated using a Superdex Peptide PC 3.2/30 column (GE) on a 1200 Infinity HPLC system (Agilent) at a flow rate of 50 μl/min ...
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bioRxiv - Cell Biology 2022Quote: ... Peptides were then desalted with Oasis HLB plates (Waters) and fractionated with an Agilent 1200 HPLC pump system (Agilent) coupled to a strong cation exchange (SCX ...
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bioRxiv - Cell Biology 2022Quote: ... In brief, peptides were loaded on a Zorbax 300-Extend-C-18 (5 µm, 4.6 × 250 mm) column (Agilent) at a constant flow rate of 1 mL/min ...
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Macrodomain Mac1 of SARS-CoV-2 Nonstructural Protein 3 Hydrolyzes Diverse ADP-ribosylated SubstratesbioRxiv - Biochemistry 2023Quote: ... Digested peptides were separated on ZORBAX 300SB-C18 MicroBore column (1.0 x 50 mm, 3.5mm, Agilent Technologies, DE, USA) with a linear gradient of acetonitrile from 0 to 50% at 1% per min with a flow rate of 20 ml/min ...
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bioRxiv - Neuroscience 2023Quote: ... Peptides were further separated using a Zorbax 300SB-C18 column (0.075×150 mm, 3.5 μm 300 Å) (Agilent Technologies) at a flow rate of 0.25 μL/min ...
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bioRxiv - Cancer Biology 2023Quote: ... The eluted peptides were then trapped in a reverse-phase column (Zorbax 300SB-C18, 0.3 × 5 mm; Agilent Technologies), and separated on a homemade column (HydroRP 2.5 µm ...
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bioRxiv - Cell Biology 2023Quote: ... TFA) and fractionated using a Superdex Peptide PC 3.2/30 column (GE) on a 1200 Infinity HPLC system (Agilent) at a flow rate of 0.05 ml/min ...
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bioRxiv - Biochemistry 2023Quote: ... TFA) and fractionated using a Superdex Peptide PC 3.2/30 column (GE) on a 1200 Infinity HPLC system (Agilent) at a flow rate of 0.05 mL/min ...
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bioRxiv - Cell Biology 2020Quote: Mito stress test: Agilent Seahorse XF Mito Stress Test Kit was used as per the standard protocol by Agilent Seahorse (https://www.agilent.com/cs/library/usermanuals/public/XF_Cell_Mito_Stress_Test_Kit_User_Guide.pdf) ...
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bioRxiv - Biochemistry 2019Quote: Site directed mutagenesis were performed according to standard protocol from the QuickChange II Site-Directed Mutagenesis kit (Agilent Technologies). The only difference was that E ...
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bioRxiv - Plant Biology 2021Quote: ... Each sample set was run together with the external standard (2-AA labeled dextran ladder, Glyko Prozyme, Hayward, CA). In each chromatogram 15 individual peaks were identified and peak assignments were made basing on glucose units (GU) ...
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bioRxiv - Systems Biology 2021Quote: ... and a mass accuracy of <1 ppm was often achieved using the standard tuning mixture (G1969-85000, Agilent Technologies). The m/z scan range was 100−2000 ...
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bioRxiv - Microbiology 2021Quote: ... and quality checked on the 4200 Tapestation using either the High Sensitivity or standard RNA ScreenTape assay (Agilent Technologies), depending on the measured RNA concentrations ...
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bioRxiv - Cancer Biology 2021Quote: ... https://ige3.genomics.unige/ch) using standard techniques RNA integrity was verified using the Agilent 2100 Bioanalyzer system (Agilent Technologies). The total RNA ribo-zero gold kit from Illumina was used for the library preparation with 1 μg or 500 ng of total RNA as input for cells (n=3 biological replicates/group ...
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bioRxiv - Bioengineering 2021Quote: ... The hydrolysis reaction products and standard oligo-galacturonates of different pectic substrates were analysed by HPLC using Zorbax-SAX column (200 ×10.0 mm, Agilent) in 0.3M sodium acetate (pH 5.0 ...
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bioRxiv - Neuroscience 2022Quote: ... Final libraries were analyzed using Bioanalyzer DNA 1000 or Fragment Analyzer Standard Sensitivity (# 5067-1504 or # DNF-473, Agilent) to estimate the quantity and validate the size distribution and were then quantified by qPCR using the KAPA Library Quantification Kit KK4835 (# 07960204001 ...
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bioRxiv - Bioengineering 2020Quote: ... Calibration was performed using PEG standards of molecular weights up to 300,000 MW (Agilent PEG calibration kit, Agilent Technologies). Molecular weight and dispersity values were calculated using LabSolutions GPC software (Shimadzu Europa GmbH) ...
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bioRxiv - Genetics 2020Quote: ... We determined efficiency of each reaction using the equation Efficiency = −1+10(−1/slope of standard curve) (Agilent Genomics). We additionally calculated ratios of transcript abundance to determine expression levels of one gene relative to another gene and to account for any differences in underlying mRNA levels among individuals ...
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bioRxiv - Cell Biology 2020Quote: Glycolysis stress test: Agilent Seahorse XF Glycolysis Stress Test Kit was used as per the standard protocol by Agilent Seahorse (https://www.agilent.com/en-us/agilent404?s=www.agilent.com/cs/library/usermanuals/public/XF_Glycolysis_Stress_Test_Kit_User_Guide.pdf) ...
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bioRxiv - Microbiology 2022Quote: ... and peak assignments in samples were made based on comparisons of retention times and accurate masses from authentic standards using MassHunter Quantitative Analysis v.10.0 software from Agilent Technologies ...
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bioRxiv - Plant Biology 2022Quote: ... was identified by known standards and using a gas chromatograph coupled to a mass spectrometer (GC-MS, Agilent 5977B) (Agilent ...
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bioRxiv - Neuroscience 2022Quote: ... The certificate of analysis attested to a 100% radiochemical purity on the basis that 99.68% of radiolabeled material co-eluted with the unlabeled reference standard on a Zorbax SX 4.6 x 250 mm column (catalog number 959990-912, Agilent Technologies Canada ...
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bioRxiv - Neuroscience 2023Quote: ... The resulting libraries were validated using the Standard Sensitivity NGS Fragment Analysis Kit (DNF-473-0500, Agilent formerly AATI) on a 12-Capillary Fragment Analyzer and quantified using Quant-it dsDNA assay kit (Cat ...
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bioRxiv - Immunology 2022Quote: ... Primary antibodies were detected by immunoperoxidase staining with the LSAB+ System and a standard DAB reaction following manufacturer’s instructions (DakoCytomation). Sections were counterstained with hematoxlyin prior to mounting and microscopy ...
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bioRxiv - Bioengineering 2023Quote: ... After calibrating and verifying the performance of the column using the LC Bio-standard (Agilent, P/N : 5190-9417), the recombinant antibody samples were diluted to 2mg/ml in 1XPBS and loaded onto a pre-conditioned column at a flow rate of 0.35ml/min ...
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bioRxiv - Developmental Biology 2023Quote: ... Periodic acid-Schiff (PAS) and hematoxylin and eosin (H&E) staining were performed using standard procedures (Dako, Glostrup, Denmark) for the assessment of normal structures.
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bioRxiv - Molecular Biology 2023Quote: ... 1000 ng of digested DNA were spiked with internal standard (D3-5mC and D2,15N2-5hmC, 250 fmol each) and subjected to analysis by LC-MS (Agilent 1260 Infinity system in combination with an Agilent 6470 Triple Quadrupole mass spectrometer equipped with an electrospray ion source (ESI)) ...
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bioRxiv - Cell Biology 2024Quote: ... Western blotting analysis was carried out using standard protocols with the antibodies listed in Table_1 and HRP-conjugated secondary antibodies (Agilent). Bands were visualised bu SuperSignal West Fempto ECL substrate (Thermo ...
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bioRxiv - Bioengineering 2024Quote: ... Immunostaining of corneas was performed according to standard procedure.52 The corneas were mounted in Dako mounting medium (Dako). We replaced the Alexa Fluor 488 dye-conjugated antibodies used in confocal microscopy with STED-dedicated secondary antibodies (abberior STAR RED ...
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bioRxiv - Cell Biology 2024Quote: ... Experiments were performed following the manufacturer’s standard protocol with two biological replicates and three technical replicates using a Synergy H1 Microplate Reader (Agilent) to read fluorescence and absorbance.
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bioRxiv - Immunology 2024Quote: ... and quality measured by capillary electrophoresis using the Fragment Analyzer and the ‘Total RNA Standard Sensitivity Assay’ (Agilent Technologies). All samples in this study showed high quality RNA Quality Numbers (RQN ...