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Citations for Agilent :
351 - 400 of 626 citations for Gentamicin Deuterated C Complex Pentaacetate Salt Contains d0 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Antigen unmasking was carried out in a DAKO PT-Link for 20 minutes at 97°C using EnVision FLEX buffer of appropriate pH for each antibody (Agilent). Then ...
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bioRxiv - Microbiology 2020Quote: ... The desorbed VOCs were subsequently collected on a cold trap at −10°C and introduced into a GC-QTOF (model Agilent 7890B GC and the Agilent 7200AB QTOF ...
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bioRxiv - Biophysics 2021Quote: ... sections were fixed for 10 min in cold acetone (−20 °C) and rinsed in TBS (Agilent, Santa Clara, CA, USA). For staining of MΦs ...
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bioRxiv - Biochemistry 2022Quote: ... Assay of formate:NAD+ oxidoreductase activity was conducted as described in the previous section at 37 °C with a BioTek Synergy Neo2 plate reader (Agilent) in buffer composed of 10 mM MES pH 6.0 ...
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bioRxiv - Bioengineering 2022Quote: ... and incubated overnight at 4 °C with primary antibodies for the glial fibrillary acidic protein (GFAP; 1:1000, Z0334, Dako), ionised calcium-binding adapter molecule 1 (IBA1 ...
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bioRxiv - Cell Biology 2022Quote: ... Sections were then heated to 97°C for 20 minutes in a high pH target retrieval solution (TRS) (K8004, Agilent) for heat-induced antigen retrieval (HIER) ...
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bioRxiv - Microbiology 2022Quote: ... 30 sec at 60°C (steps 3 and 4 were repeated 40 times) was done with the AriaMX real-time PCR System (Agilent).
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bioRxiv - Microbiology 2022Quote: 2 μl of extracted metabolite samples were separated on a Cogent Diamond Hydride Type C Column of 1200 liquid chromatography (Agilent) which was coupled to an Agilent Accurate-Mass 6220 Time-of-Flight spectrometer ...
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Flaviviruses alter endoplasmic reticulum-mitochondria contacts to regulate respiration and apoptosisbioRxiv - Microbiology 2023Quote: ... were hydrated overnight at 37°C in a non-CO2 incubator with 200 µl/well of the Seahorse XF calibrant (Agilent). The day of the assay ...
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bioRxiv - Neuroscience 2022Quote: ... Retinal disks were incubated for 30 minutes at 37°C prior to running the assay using a Seahorse XF96 Analyzer (Agilent). During the assay ...
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bioRxiv - Immunology 2022Quote: ... and incubated at 37°C for 1 h before the plate was placed into an Extracellular Flux Analyzer XFe96 (Seahorse, Agilent) for measuring the extracellular acidification rate (ECAR ...
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bioRxiv - Physiology 2022Quote: ... sections were dewaxed and epitope retrieval was performed using Citrate buffer pH6 for α-SMA and Ter119 or Tris- EDTA buffer pH9 for HMOX1 in all cases for 20 min at 97°C using a PT Link (Dako, Agilent) or with ER2 buffer (AR9640 ...
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bioRxiv - Cancer Biology 2022Quote: The sensor cartridge plate was hydrated with sterile distilled water overnight at 37°C in a CO2-free incubator and was replaced with a calibrant solution (Agilent) for 1 hour ...
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bioRxiv - Physiology 2024Quote: ... tissues were incubated with primary antibody over night at 4°C (guinea pig anti-insulin at 1:4, diluted in 1% goat serum/PBS, Agilent). This was followed by 1-hour incubation with fluorophore-conjugated secondary antibodies (Cy3-anti-guinea pig ...
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bioRxiv - Plant Biology 2024Quote: ... Root length was measured 24h upon UV-C exposure (300, 600 and 900 J/m2) using the Stratalinker 2400 (Stratagene). The relative root growth was calculated as followed ...
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bioRxiv - Microbiology 2024Quote: ... Extracted metabolites were separated on a Cogent Diamond Hydride Type C column of 1200 liquid chromatography coupled to an Mass 6220 TOF spectrophotometer (Agilent). Ion abundances of metabolites were estimated using Profinder 8.0 and log2 fold changes were analyzed and plotted using Gene Cluster 3.0 and Java Treeview.
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bioRxiv - Microbiology 2024Quote: The frozen samples were quickly thawed and injected into a refrigerated (1°C) HPLC system (Agilent Infinity 1260, Agilent Technologies), through a porcine pepsin column (≥ 3200 units/mg ...
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bioRxiv - Microbiology 2024Quote: The frozen samples were quickly thawed and injected into a refrigerated (1°C) HPLC system (Agilent Infinity 1260, Agilent Technologies), through a porcine pepsin column (≥ 3200 units/mg ...
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bioRxiv - Neuroscience 2024Quote: ... Antigen retrieval treatment (95°C for 20 min) was added to expose masked epitopes with Target Retrieval Solution (Agilent Dako). After an hour blocking with 10% serum at RT ...
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bioRxiv - Neuroscience 2024Quote: ... Antigen retrieval treatment (95°C for 20 min) was added to expose masked epitopes with Target Retrieval Solution (Agilent Dako). After an hour blocking with 10% serum at RT ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibody incubation was performed overnight at 4 °C with rabbit monoclonal anti-PSyn EP1536Y diluted 1:320,000 in DAKO antibody diluent (Agilent #S0809). After three 5-min washes with PBST ...
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Synthetase and Hydrolase Specificity Collectively Excludes 2’-Deoxyguanosine from Bacterial AlarmonebioRxiv - Microbiology 2024Quote: All hydrolysis assays were performed in 96-well plates at 30 °C in a BioTek Synergy H1 microplate reader (Agilent). Production of GDP ...
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bioRxiv - Immunology 2024Quote: ... Epitope retrieval was then performed at 95 °C for 10 min at pH 9 (Dako Target Retrieval Solution, S236784-2) in a Lab Vision PT Module (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... Epitope retrieval was then performed at 95 °C for 10 min at pH 9 (Dako Target Retrieval Solution, S236784-2) in a Lab Vision PT Module (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2023Quote: ... antigen retrieval using sodium citrate buffer (10 mM, pH 6) was performed for 15 min at 110 °C in a DAKO Pascal pressure chamber (Agilent). After blocking in PBS supplemented with 10% goat serum (Sigma Aldrich ...
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bioRxiv - Biophysics 2024Quote: ... using a 3 × 3 mm black walls quartz cell at 25 °C on an Agilent Cary Eclipse spectrofluorometer (Agilent Technologies) equipped with a thermostatted cell holder attached to an Agilent PCB 1500 water Peltier system ...
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bioRxiv - Biochemistry 2024Quote: ... and subsequently cloned into a pET-SUMO expression vector with an additional C-terminal hexahistidine tag cloned in using QuikChange (Agilent).
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bioRxiv - Cell Biology 2024Quote: hAC were fixed with 4% formalin and blocked for 1 h at 37°C (Protein-Block serum free, X0909 Dako Agilent). COL2 antibody (MA5-13026 ...
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bioRxiv - Cell Biology 2024Quote: ... Sections were progressively rehydrated before antigen retrieval for 30 min at 95°C in 1X Antigen Retrieval Buffer (Dako, 51699). Samples were incubated in perm-block buffer (5% donkey serum ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were stored at - 80°C until crosslinking (still frozen) with 400 mJ/cm2 UVC using a Stratalinker 2400 (Stratagene).
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bioRxiv - Cell Biology 2023Quote: ... Cells were then incubated in a 0% CO2 chamber at 37 °C for 1 hour before being placed into a Seahorse XFe24 Analyzer (Agilent). For oxygen consumption rate (OCR ...
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bioRxiv - Cell Biology 2022Quote: ... Plates were immediately centrifuged at 2 000 x g for 20 min at 4 °C and left on ice until insertion into the Seahorse XFe96 Analyzer (Agilent) after the cartridge plate calibration cycle on the analyzer was complete ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated overnight at 4°C in primary antibodies (RFP from Chromotek 5f8-100 at 1:200, GFAP from Agilent DAKO Z033429-2 at 1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated overnight at 4°C in primary antibodies (RFP from Chromotek 5f8-100 at 1:200, GFAP from Agilent DAKO Z033429-2 at 1:500 ...
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bioRxiv - Plant Biology 2023Quote: ... The HPLC was equipped with a C-18 column (4.6 mm by 150 mm column, Zorbax Eclipse XDB-C18, Agilent Technologies) and inline radio-detector (FlowStar LB 513 ...
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bioRxiv - Biophysics 2023Quote: Yeast eIF4G was purified as described previously.46 A pTYB2 vector encoding a C-terminal fusion of eIF4G with an intein and chitin-binding domain was transformed into BL21 CodonPlus RIL cells (Agilent). Protein expression was induced with 0.5 mM IPTG for overnight at 16 °C ...
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bioRxiv - Microbiology 2023Quote: Pooled gradient fractions were diluted 1:2 with ice-cold PBS and tumbled overnight at 4°C with 50 µl Strataclean resin (Agilent). Beads were pelleted at 600 RCF for 5 min at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... Shimadzu LC-10AT; autosampler: HiP sampler G1367A, T = 4°C, 10 µL injection; flow rate: 1 mL/min; column: Agilent Zorbax Eclipse XDB-C18 80Å ...
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bioRxiv - Biochemistry 2023Quote: ... 10μL of purified protein was injected to a C-18 column (Agilent Zorbax 300SB-C18, 5 μM, 2.1 x 150mm) without dilution or buffer exchange ...
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bioRxiv - Cell Biology 2022Quote: ... Splitless injection was performed at 270°C in a 30m + 10m x 0.25mm DB-5MS+DG column (Agilent J&W). Helium was used as the carrier gas ...
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bioRxiv - Cell Biology 2023Quote: ... Splitless injection was performed at 270°C in a 30m + 10m x 0.25mm DB-5MS+DG column (Agilent J&W) and helium was used as the carrier gas ...
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bioRxiv - Biophysics 2023Quote: ... emission 353 nm) of 25 μM PrP was acquired upon CuCl2 titration at 25°C in a Cary Eclipse Fluorescence Spectrophotometer (Agilent) with slits of 5 nm (excitation ...
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bioRxiv - Immunology 2023Quote: ... Antigen retrieval was then performed for 20 minutes at 95°C using Lecia Epitope Retrieval Buffer 2 followed by treatment with Dako serum-free protein block (X090930-2, Agilent Dako) for 15 minutes to prevent non-specific binding of the antibody ...
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bioRxiv - Neuroscience 2023Quote: ... and kept at 4°C overnight with primary antibodies (chicken anti-GFP, Aves Labs, 1:4000; rabbit anti-GFAP, Agilent Pathology Solutions ...
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bioRxiv - Bioengineering 2023Quote: ... read height of 7 mm and at 37 °C with excitation at 534-554 nm wavelength using a Cytation5 (Agilent). All values were subtracted by a control with only 8FN microgels and no cells.
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bioRxiv - Bioengineering 2023Quote: ... read height of 7 mm and at 37 °C with excitation at 315-335 nm wavelength using a Cytation5 (Agilent). All values were subtracted by a control with only 8FN microgels and no cells.
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bioRxiv - Cell Biology 2023Quote: ... Fluorescence was analyzed in a thermostat controlled (37°C) and magnetically-stirred Cary Eclipse fluorescence spectrophotometer (Agilent Technologies, Milano, Italy) or using a confocal microscope (LSM Carl Zeiss ...
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bioRxiv - Molecular Biology 2023Quote: ... Chromatographic separation was carried out at 40°C using an Agilent 1260 Infinity II series UPLC (Agilent, Santa Clara, USA) over a 4 minute gradient elution (KNOWNS_AM190319.m ...
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bioRxiv - Plant Biology 2023Quote: ... Enzyme activity was determined by the consumption of NADH at 340nm using a diode array spectrophotometer at 25°C (Agilent).
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bioRxiv - Microbiology 2023Quote: ... a mutant version of each of the hsdR1 had a unique BamHI site inserted by site directed mutagenesis of a C at position 1033 of hsdR1 to a G using oligonucleotide directed mutagenesis (5’-CATCAGAGACTTTTTTAGCGGATCCAACCTAAACAAAAAGAC-3’) (Quick-Change Lightning. Agilent). The mutated site is shown in bold and italics and the generated BamHI site is underlined ...