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Citations for Agilent :
551 - 600 of 626 citations for Gentamicin Deuterated C Complex Pentaacetate Salt Contains d0 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... and F272fs287X (c.813del) in the GABAAR α1 subunit were constructed using a QuikChange II site-directed mutagenesis Kit (Agilent Genomics, #200523), and the cDNA sequences were confirmed by DNA sequencing.
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bioRxiv - Cell Biology 2024Quote: ... D69T mutant containing an N-terminal His14-Sumo tag and a C-terminal myc tag were expressed in BL21-CodonPlus(DE3)-RIPL Competent cells (Agilent Technologies #230280). Bacterial cells were grown in terrific broth (supplemented with K-Phos salt solution and Kanamycin ...
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bioRxiv - Microbiology 2020Quote: ... The desorbed VOCs were subsequently collected on a cold trap at −10°C and introduced into a GC-QTOF (model Agilent 7890B GC and the Agilent 7200AB QTOF, USA) by heating the cold trap for 10 min to 280°C ...
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bioRxiv - Developmental Biology 2020Quote: ... sections were permeabilized with 0.25% TritonX-100 in PBS and autoclaved (121°C, 10 min) with Target Retrieval Solution (Agilent Technologies, Santa Clara, California). Sections were incubated with Blocking One Histo (Nacalai Tesque Inc. ...
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bioRxiv - Microbiology 2021Quote: ... Supernatant was harvested by centrifugation at 1500 rcf for 4 minutes at 23°C and filtered using a Captiva 96 well 0.2 μm filter plate (Agilent Technologies, Santa Clara, CA) prior to titer measurement by RP-UHPLC.
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bioRxiv - Physiology 2021Quote: Oxygen consumption was measured at 37°C from isolated mouse and human islets after overnight recovery using a Seahorse XF24 analyzer (Agilent, Santa Clara, CA). Islets were seeded at a density of 75 islets/well ...
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bioRxiv - Microbiology 2021Quote: ... from the pIdsBB expression system containing a C-terminal GFPmut2 fusion (Gibbs et al., 2008) using error-prone PCR with the GeneMorph II Random Mutagenesis Kit (Agilent, Santa Clara, CA) and ligated back into the same pIdsBB expression vector using the restriction enzymes SacI and BamHI ...
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bioRxiv - Microbiology 2020Quote: ... the epitopes were unmasked by heating at 95°C for 30 min prior to detection on a Dako OMNIS™ automate (Agilent Technologies, USA). When indicated ...
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bioRxiv - Biophysics 2021Quote: ... incubated for 16 h at 4 °C and purified by HPLC on a Zorbax C3 column with dimensions of 21 × 150 mm (Agilent, Santa Clara, CA) using a gradient of 20-90 % acetonitrile in water with 0.1 % trifluoroacetic acid (TFA ...
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Retinal affectation in Huntington’s disease mouse models concurs with a local innate immune responsebioRxiv - Neuroscience 2023Quote: ... and incubated overnight in a humid chamber at 4°C with rabbit anti-glial fibrillary acidic protein (GFAP) antibody (1:500, Z0334, DAKO, Agilent, Glostrup, Denmark), rabbit anti-ionized calcium binding adaptor molecule 1 (Iba-1 ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were then incubated at 37°C in a non-CO2 incubator for 1 hour and subsequently loaded into the Seahorse XFe96 Analyzer (Agilent Technologies, Seahorse Bioscience) when prompted ...
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bioRxiv - Cell Biology 2023Quote: ... the fibers were placed in an incubator at 37 °C with no CO2 and the medium was replaced with XF Base media (Agilent, Santa Clara, CA) with glucose (10 mM) ...
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bioRxiv - Cell Biology 2023Quote: ... the fibers were placed in an incubator at 37 °C with no CO2 and the medium was replaced with XF Base media (Agilent; Santa Clara, CA), with sodium pyruvate (1 mM ...
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bioRxiv - Evolutionary Biology 2023Quote: Enzymes were assayed at 25 °C by recording the change in absorbance at 340 nm for 300 seconds on a Cary 100 Bio (Agilent, Santa Clara, CA) in 50 mM Tris ...
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bioRxiv - Synthetic Biology 2023Quote: ... and left to grow at 30 °C and 600 rpm until all cultures reached OD600 above 0.3 measured in a Biotek Synergy H4 Multimode (Agilent, Santa Clara, CA, USA) microplate reader ...
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bioRxiv - Synthetic Biology 2022Quote: ... 5’- CTT TTT CAA ATT GGG GGT GTG ACC ATG CAG CGC TAG CCG CAG C −3’) using the GeneMorph II epPCR kit (Agilent, Singapore, Cat: STR_200550). The epPCR profile is as follows ...
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bioRxiv - Synthetic Biology 2022Quote: ... the oxidation of NADH was followed at 340 nm and 30 °C on a Cary 60 UV-Vis photospectrometer (Agilent, Santa Clara, USA) in quartz cuvettes with a pathlength of 10 mm (Hellma Optik GmbH ...
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bioRxiv - Synthetic Biology 2022Quote: ... and then optical density at 600 nm (OD600) was monitored with a Biotek Synergy H1M at 30°C for 48 hours with fast continuous shaking (Agilent, Santa Clara, CA).
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bioRxiv - Cancer Biology 2023Quote: ... the plasmids containing the C- and N-terminally His-tagged human ACSS2 DNA were transformed into BL21 (DE3) RIL competent cells (Agilent Technologies, Wilmington, DE) and were expressed in auto-inducing media ZYP-5052 overnight at 15°C with shaking at 225 rpm ...
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bioRxiv - Biochemistry 2024Quote: Spectra of dark-adapted AsLOV2 and NcVVD variants were acquired at 22°C on a diode-array absorbance spectrophotometer (Agilent Technologies, model 8453) equipped with Peltier temperature control (Agilent Technologies ...
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bioRxiv - Bioengineering 2024Quote: ... and the plate was incubated at 37 °C and 5% CO2 in a BioSpa live cell analysis system (Agilent Technologies, Santa Clara, CA) for 48 hours (Fig ...
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bioRxiv - Developmental Biology 2024Quote: ... carefully dried using paper tissues and immediately analyzed using an Agilent Technologies GC system (7890 A GC System and 5975 C inert XL MSD with Triple-Axis-Detector, Agilent Technologies Deutschland GmbH) (Potter et al ...
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bioRxiv - Microbiology 2024Quote: ... of 0.1 and incubated for 100 h at 30 °C with linear shaking at 567 cycles per minute using an EPOCH microplate reader (Agilent, Santa Clara, CA). The OD600 was measured continuously.
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bioRxiv - Microbiology 2023Quote: ... Plate was shaken continuously at 30°C and OD600 was read every 20 minutes in a BioTek Epoch 2 Microplate Spectrophotometer (Agilent, Santa Clara, CA). MMC (0.5 μg/ml ...
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bioRxiv - Bioengineering 2024Quote: ... and the plates were incubated at 37°C and 5% CO2 in a BioSpa live cell analysis system (Agilent Technologies, Santa Clara, CA) for 45 hours.
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bioRxiv - Neuroscience 2023Quote: ... Cells were incubated for 1h at RT before overnight incubation at 4°C with primary antibodies: Glial Fibrillary Acidic Protein (GFAP) (1/500 Dako Cat. Number Z0334); AQP4 (1/500 ...
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bioRxiv - Plant Biology 2024Quote: ... We used a Zorbax SB-C18 5 μm 4.6 x 250 mm column at 35°C on a 1260 Infinity HPLC instrument with a fluorescence detector (Agilent Technologies, Santa Clara CA) using an excitation wavelength of 340 nm and emission wavelength of 510 nm ...
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bioRxiv - Cell Biology 2022Quote: Analysis of mitochondrial functions in HAP 1 WT and HAP 1 Δ(c+δ) cells was performed on Seahorse XFe24 (Agilent Technologies, USA) as described previously (Nichols et al. ...
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bioRxiv - Bioengineering 2022Quote: ... and the plates were incubated at 37 °C and 5% CO2 in a BioSpa live cell analysis system (Agilent Technologies, Santa Clara, CA) for 45 hours.
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bioRxiv - Neuroscience 2022Quote: ... and the plate was incubated at 37°C for 30 minutes before reading at 562 nm on a Synergy plate reader (Agilent, Santa Clara, CA). Using the resulting protein concentration ...
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bioRxiv - Neuroscience 2023Quote: ... Afterwards the sections were incubated in a humidified chamber (overnight 4°C) in a peroxidase conjugated immunoglobulin against UEA (DAKO, P289; 1:50). Finally ...
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bioRxiv - Neuroscience 2023Quote: ... Antigen □ retrieval was performed by incubating the tissue for 2 h at 70 °C in Target Retrieval Solution (Dako, Santa Clara, CA, USA) using a 1:10 dilution in demineralized water ...
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bioRxiv - Neuroscience 2024Quote: ... they were incubated overnight at 4°C with the respective primary antibodies: (i) polyclonal rabbit anti-GFAP (1:2000; Dako, Cat. No. Z0334); (ii ...
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bioRxiv - Immunology 2024Quote: ... Slides were baked in an oven at 70°C for 60 minutes and then loaded onto an automated stainer (Leica Bond III for CD4 staining and Dako Omnis (Dako / Agilent) for BCL6 staining ...
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bioRxiv - Developmental Biology 2024Quote: Perch at hatch were incubated at 25°C for 1h before the Seahorse XF Cell Mito Stress bioassay (Agilent, Santa Clara, United States). The Seahorse XF Calibrant fluid was used to hydrate the sensor plate for 24 h at 25°C before the assay ...
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bioRxiv - Cell Biology 2020Quote: ... followed by overnight incubation at 4 °C with α-α-SMA (1:400, mouse monoclonal, clone 1 A4, DAKO Agilent Technologies, Santa Clara, USA) or α-CD68 (1:2000 ...
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bioRxiv - Cell Biology 2020Quote: ... followed by overnight incubation at 4 °C with α-α-SMA (1:400, mouse monoclonal, clone 1 A4, DAKO Agilent Technologies, Santa Clara, USA) or α-CD68 (1:2000 ...
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bioRxiv - Cell Biology 2020Quote: ... Germany) followed by overnight incubation at 4 °C with specific primary antibodies: polyclonal rabbit anti-human AAT (1:800) (DAKO A/S, Glostrup, Denmark), mouse monoclonal anti-AAT polymer antibody (clone 2C1 ...
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bioRxiv - Cell Biology 2022Quote: ... SNAP-tagged Cdc122FH1-Fus1FH2 and Cdc122FH1-Fus1R1054EFH2 constructs (including the C-terminal tail) were expressed in Escherichia coli strain BL21-Codon Plus (DE3) RP (Agilent Technologies, Santa Clara, CA) with 0.5 mM isopropyl β-D-1-thiogalactopyranoside for 16 h at 16 °C ...
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bioRxiv - Physiology 2020Quote: ... blocked with 3% BSA in PBS1 and incubated overnight at 4°C with primary polyclonal antibodies for glucagon and insulin (Dako, Agilent, Santa Clara, CA, USA) (Table S8) ...
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bioRxiv - Biochemistry 2020Quote: ... linear gradient from 5% to 35% ACN in 50 mM TEAAc pH 7 over 15 min at 50 °C, Agilent Technologies Series 1200 HPLC). The collected fractions were freeze dried 3 times ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated overnight at 4°C in primary antibody (2% NGS in TBST) with either rabbit anti-GFAP (1:2000, Agilent Cat# Z0334, RRID: AB_10013382), rabbit anti-synaptophysin (IgG ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Plasmid DNA mutations in the C-terminus of PAR2 were created using QuikChange XL Multi Site-Directed Mutagenesis kit (Agilent Technologies, Mississauga, ON, Canada) to generate all mutants described in this study ...
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bioRxiv - Physiology 2021Quote: ... The cells were incubated in this medium for 45 minutes at 37°C in a non-CO2 incubator and then transferred to a Seahorse XFe24 extracellular flux analyser (Agilent, Santa Clara, CA, USA) maintained at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... samples were incubated with FSCN1 primary antibodies at 4°C overnight and the color of the sample was developed using the DAKO ChemMate Envision Kit HRP (Dako-Cytomation, Carpinteria, CA, USA) followed by counterstaining with hematoxylin ...
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bioRxiv - Cell Biology 2023Quote: ... at maximum speed for 10 minutes at 4°C prior to injection into a hydrophilic interaction (HILIC) liquid chromatography (LC) and high-resolution mass spectrometer (QTOF-6545, Agilent, Santa Clara, CA, USA). Metabolite identification and quality checks for polar metabolomics was performed using QTOF MassHunter Quant software (Agilent ...
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bioRxiv - Cell Biology 2023Quote: ... Tissue sections on slides were baked at 60°C for 1 h and then deparaffinized and retrieved on Dako Omnis fully automated IHC platform (Agilent, Santa Clara, CA, USA) using EnVision FLEX target retrieval solution ...
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bioRxiv - Cell Biology 2022Quote: ... and fluorescence intensity was measured at 340/440 nm over 1 hr at 37 °C using a BioTek Synergy HTX (Agilent, Santa Clara, CA, USA).
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bioRxiv - Cell Biology 2022Quote: ... The fluorogenic substrate was added to each well of a 96-well plate and the fluorescence intensity was measured at 340/440 nm over a 1 hr period at 37 °C using a Bio-Tek Synergy HTX (Agilent, Santa Clara, CA, USA) plate reader.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The well plate was then inserted into the humidified chamber at 37°C of an Agilent Lionheart FX Automated Microscope (Agilent Technologies, Santa Clara, CA). A band pass filter (Thorlabs ...