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Citations for Agilent :
501 - 550 of 626 citations for Gentamicin Deuterated C Complex Pentaacetate Salt Contains d0 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... at 40°C with an InfinityLab Poroshell 2.7 µM EC-C18 pre- column (3 x 5 mm; Agilent Technologies, Waldbronn, Germany) was used ...
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bioRxiv - Plant Biology 2023Quote: ... One hundred micrograms of precipitated protein sample were digested with trypsin and 30 µg of the resulting peptides desalted with PepClean C-18 Spin Columns (Agilent Technologies) according to manufacturer recommendations as previously described (Martinez-Marquez et al ...
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bioRxiv - Neuroscience 2023Quote: ... for 60 min and incubated overnight at 4°C with primary antibodies diluted in DAKO Antibody Diluent (Agilent, cat. #S080983-2). Sections were washed in PBS (3 x 5 min ...
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bioRxiv - Neuroscience 2023Quote: Vibratome cut (free floating) tissue was heated to 70°C in 1x Daco Target Retrieval solution (DakoTarget Retrieval Solution, pH 6.1, Dako, code. S2367) for 15 min and left to cool at room temperature (RT) ...
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bioRxiv - Microbiology 2023Quote: ... The Seahorse XFp extracellular flux cartridge was hydrated with sterile water and incubated overnight at 30 °C and Seahorse XF calibrant solution (Agilent Technologies). After this ...
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bioRxiv - Bioengineering 2023Quote: ... The electrode temperature was controlled by submerging in 70 °C PBS solution with a P4010 thermal meter (Dostmann electronic, Germany) for temperature monitoring and a 34401A digit multimeter (Agilent, USA) for electrical resistance measurement ...
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bioRxiv - Biochemistry 2024Quote: ... sections were deparaffinized, dehydrated and subjected to antigen retrieval (20 min incubation at 98 °C in citrate buffer, pH 6 (Agilent Dako) for CD8 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... was fragmented and hybridized to a microarray for 17 h while rotating at 10 rpm at 65 °C in a hybridization oven (Agilent Technologies). The hybridized microarray was washed and scanned according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: The purified RNAs in the 96-well PCR plate (Axygen Scientific, PCR-96-FS-C) were transferred to a 0.3 mL 96-well plate (Agilent, 5043-9313) for hydrolysis in a 40 µL of an enzyme cocktail containing 10 U benzonase (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... Log-phage cultures were inoculated into specified media and monitored for growth rate every 15 min at OD600 for 24 or 72 hours at 37°C under static conditions using BioTek Synergy H1 (Agilent Technologies).
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bioRxiv - Neuroscience 2024Quote: ... The resulting supernatant was then separated and stored at -80°C for further colorimetric measurements with a microplate reader (BioTek Synergy H1, Agilent, USA).
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bioRxiv - Molecular Biology 2024Quote: ... The ligation reaction was incubated at 65 °C for 10 min before transformation into XL1-Blue Supercompetent cells (Agilent Technologies, USA). Clones were selected via test restriction analysis on a 0.8% native agarose gel (NAG) ...
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Development of ketobenzothiazole-based peptidomimetic TMPRSS13 inhibitors with low nanomolar potencybioRxiv - Pharmacology and Toxicology 2024Quote: ... R560 and K561 and addition of a 6xHis tag at the c-terminal using the QuikChange Lightning site-directed mutagenesis kit (Agilent, 210518).
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bioRxiv - Molecular Biology 2024Quote: ... 0.6 mg mL−1 enzyme with 2 mM nicotine was incubated at 30°C for 3 days before mass spectrometry on 6545 Q-TOF LC/MS system (Agilent, USA) in positive mode ...
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bioRxiv - Synthetic Biology 2024Quote: ... and the stock solution was stored anaerobically at 4°C after its concentration was verified using inductively coupled plasma mass spectrometry (ICP-MS; Agilent 7800). Negligible oxidation of arsenite to arsenate was confirmed prior to use by high-performance liquid chromatography (HPLC ...
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bioRxiv - Microbiology 2024Quote: ... Plates were stacked without lids and incubated at 37 °C for 24 h in a stacker-incubator system (Biostack 4, Agilent BioTek) connected to a plate reader (Epoch 2 ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were immediately injected onto the LC system maintained at 0°C coupled to an Agilent Infinity II 1260/1290 UPLC (Agilent Technologies). The LC outlet was directly interfaced with the ESI source of timsTOF Pro equipped with PASEF (Bruker Daltonics) ...
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Loss of TMEM55B modulates lipid metabolism through dysregulated lipophagy and mitochondrial functionbioRxiv - Cell Biology 2024Quote: ... and incubated with reagents for 60 – 90 minutes at 37°C and absorbance (OD570 nm) was measured in the kinetic mode using BioTek Synergy H1 Plate Reader (Agilent Technologies). Lipase activities were calculated as nmol/min/mL based on the standard curve.
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bioRxiv - Cell Biology 2024Quote: ... the growth medium in the cell culture microplate was replaced with a pre-warmed (37°C) assay medium including Seahorse XF base medium (Agilent Technologies), 1 mM Pyruvate (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... samples were dewaxed and antigen retrieval process using citrate buffer pH6 for 20 min at 97°C using a PT Link (Dako – Agilent) was performed ...
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bioRxiv - Cell Biology 2024Quote: ... Splitless 1 µl injection (injection temperature 250°C) onto a 40 m × 0.25 mm VF-5ms + EZ Guard column (Agilent J&W) was used with helium as the carrier gas ...
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bioRxiv - Microbiology 2021Quote: ... The test tubes were incubated for 18-24 hours at 37 °C after which turbidity was observed and optical density recorded using “Cary Series UV/Vis” (Agilent Technologies, Germany). The lowest concentration that completely inhibited the growth of the test organisms as observed by the no growth when the concentration with the least optical density is plated out on nutrient agar was reported as the MIC ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and the consumption of NADPH was followed at 30 °C and 340 nm (Δε=6.22 mM-1 cm-1) with a Cary 60 UV-Vis spectrophotometer (Agilent Technologies, Germany).
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bioRxiv - Evolutionary Biology 2020Quote: ... The reaction was started with different concentrations of 3-butenoic acid and the consumption of NADPH was followed at 30 °C and 340 nm (Δε=6.22mM-1 cm-1) with a Cary 60 UV-Vis spectrophotometer (Agilent Technologies, Germany).
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bioRxiv - Evolutionary Biology 2020Quote: ... and stored at −80°C after verifying concentration and quality by spectrophotometer (Nanodrop, Thermo scientifics) and a Bioanalyzer (RNA 6000 Nano Kit, Agilent Technologies Inc). We produced libraries for these 21 individuals using the TruSeq RNA Library Prep Kit v2 (Illumina ...
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bioRxiv - Developmental Biology 2021Quote: ... The mutated forms of the amplified sequence in which the potential m6A sites were replaced by cytidine (C) or guanine (G) were constructed using the QuikChange II XL Site-directed Mutagenesis Kit (Agilent Technologies, 200518). The fragments were separately inserted into the pCambia2300 vector ...
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bioRxiv - Cancer Biology 2020Quote: ... and cRNA was hybridized with ArrayXS Human (Oaklabs, Germany) at 65 °C for 17 hours using the Agilent Gene Expression Hybridization Kit (Agilent Technologies, USA), washed once with Agilent Gene Expression Wash Buffer 1 for one minute at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... blocked using 10% Normal Goat Serum (NGS) and incubated overnight with the following primary antibodies in 5% NGS overnight at 4°C: rabbit anti-GFAP (DAKO; #Z0334;1:1000), rat anti-L1-CAM (Millipore ...
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bioRxiv - Plant Biology 2021Quote: ... at 37⁰C (1:50 protein/trypsin ratio) and cleaned up with a C18 column (OMIX C18, Agilent, Santa Clara, CA, USA). Nano-liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS ...
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bioRxiv - Cancer Biology 2020Quote: ... slides with PCa tissue samples (n=144) were incubated at 60°C for 2 hours followed by target retrieval in a PT Link instrument (Agilent DAKO, PT200) using EnVision FLEX Target Retrieval Solution ...
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bioRxiv - Molecular Biology 2022Quote: ... they were first permeabilized using an acetone freeze step for 7 min at -20° C before staining with guinea-pig anti-insulin (Dako, Carpinteria, CA), Alexa Fluor 488 or 647 goat anti-guinea-pig (Thermo Fisher Scientific ...
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bioRxiv - Pathology 2020Quote: ... Deparaffinization and antigen retrieval were performed in Target Retrieval Solution with pH 9 (ACE2) or pH 6 (Ki-67) at 97°C for 20 min using the PT Link platform (Dako, Glostrup, Denmark). Subsequently ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... column maintained at ambient temperature (30ΰC) coupled to a quadrupole time-of-flight (Q-TOF) mass spectrometer (Q-TOF-LC/MS 6510 classic G6150A, Agilent technologies, USA) with a dual ESI as ion source ...
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bioRxiv - Microbiology 2021Quote: ... for 1 hour at room temperature or overnight at 4°C using the alkaline phosphatase-anti-alkaline-phosphatase (APAAP) technique according to the manufacturer’s protocol (Dako, Carpinteria, CA, USA). All antibodies were diluted in TBS with 0.1% BSA ...
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bioRxiv - Developmental Biology 2021Quote: ... Tissue sections were then incubated overnight at 4°C with primary antibodies (Extended Table 2) in Antibody Diluent (Agilent Dako, S080983-2). Following a wash step ...
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bioRxiv - Developmental Biology 2021Quote: ... Tissue sections were then incubated overnight at 4°C with primary antibodies (Extended Table 2) in Antibody Diluent (Agilent Dako, S080983-2). Following a wash step ...
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bioRxiv - Developmental Biology 2022Quote: ... The PVDF membranes were blocked in 5 % nonfat dry milk diluted in Tris-buffered saline with 0.05 % Tween-20 for 1 h at room temperature and then incubated overnight at 4 °C with antibodies against FN (1:70000, rabbit anti-fibronectin, DAKO, Hamburg, Germany). After washing with TBS-T ...
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bioRxiv - Cancer Biology 2022Quote: ... was carried out by drying silica NPs at 37°C for 48 hours and finally placing the dry sample onto a Cary 630 FTIR spectrometer (Agilent Technologies Ltd) for the measurement of transmittance spectra ...
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bioRxiv - Biophysics 2022Quote: Fluorescence titrations were carried out at 25 °C using 1 × 0.3 cm path-length cuvettes in a Cary Eclipse spectrofluorimeter (Agilent Technologies, Waldbronn, Germany). Experiments were carried out in 20 mM K-phosphate ...
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bioRxiv - Microbiology 2023Quote: ... Fluorescence was measured at 50°C over 1 h at excitation 360 nm emission 460 nm on a BioTek Synergy HTX Multimode Reader (BioTek, Agilent, Tx, USA). The reaction rate was calculated as the maximum velocity over 12 sequential readings ...
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Retinal affectation in Huntington’s disease mouse models concurs with a local innate immune responsebioRxiv - Neuroscience 2023Quote: ... and incubated overnight in a humid chamber at 4°C with rabbit anti-glial fibrillary acidic protein (GFAP) antibody (1:500, Z0334, DAKO, Agilent, Glostrup, Denmark), rabbit anti-ionized calcium binding adaptor molecule 1 (Iba-1 ...
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bioRxiv - Molecular Biology 2023Quote: ... seedlings were exposed on the plates to 8 kJ/m2 UV-C light in a Stratalinker 2400 (Stratagene, La Jolla, California, US) and transferred back into growth chamber for 5 h ...
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bioRxiv - Cancer Biology 2023Quote: ... Tumor xenograft slides were incubated at 60 °C for 2 h followed by antigen retrieval in a PT Link instrument (Agilent DAKO, PT200) using EnVision FLEX Target Retrieval Solution ...
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bioRxiv - Physiology 2023Quote: ... slides (4 μm thickness) were baked at 60° C for 45 min and treated with a low pH target retrieval solution (#DM829; Dako, Glostrup, Denmark) in a DAKO PT Link HIER machine (PTlink 200 ...
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bioRxiv - Genomics 2023Quote: ... and the size and concentration of the finished PCHi-C libraries was assessed by high-sensitivity DNA ScreenTape Analysis (Agilent #5067-5584) on an Agilent 2200 Tapestation.
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bioRxiv - Microbiology 2024Quote: ... An Agilent 1200 Series HPLC system was used with a refraction index detector (RID) at 50°C (Agilent, Santa Clara, CA, USA).
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bioRxiv - Plant Biology 2024Quote: ... 1.8 µm column at a flow rate of 0.45 mL/min and a column temperature of 40°C (Agilent Technologies, Waldbronn, Germany). The separation was performed with a gradient of solvent A (water ...
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bioRxiv - Pathology 2024Quote: ... The tissues were rinsed thrice with wash buffer on a shaker and incubated for 30 minutes at 37°C with secondary Ab (goat anti-rabbit HRP conjugated, Dako Envision K4003), followed by three more rinses with wash buffer on a shaker ...
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bioRxiv - Cell Biology 2024Quote: ... and F272fs287X (c.813del) in the GABAAR α1 subunit were constructed using a QuikChange II site-directed mutagenesis Kit (Agilent Genomics, #200523), and the cDNA sequences were confirmed by DNA sequencing.
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bioRxiv - Biochemistry 2024Quote: ... containing the assembled ribosomes from 25 µL mixtures and 5 nM DNA template encoding sfGFP were incubated at 37 °C in Mx3005P (Agilent Technologies, USA), and sfGFP fluorescence was monitored during incubation.