Labshake search
Citations for Agilent :
3251 - 3300 of 8383 citations for Human Adenosylhomocysteinase Like 1 AHCYL1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2024Quote: RNA samples were prepared using an Absolutely RNA microprep kit (cat.no 400805, Agilent technologies) and Nanostring profiling was performed using nCounter technology as per the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2024Quote: ... measured using the Bioanalyser 2100 with the corresponding RNA 6000 Nano Kit (Agilent, USA), as per manufacturer instructions.
-
bioRxiv - Cancer Biology 2024Quote: ... Point mutations were introduced using the QuikChange Lightning Site Directed Mutagenesis Kit (Agilent #210518) according to the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2024Quote: ... Library size distribution and concentration were measured using BioAnalyzer High Sensitivity DNA kit (Agilent) and NEB quantification kit ...
-
bioRxiv - Cancer Biology 2024Quote: ... RNA was quality-accessed with an Agilent 2100 Bioanalyzer (Agilent RNA 6000 Nano Kit) with RNA integrity number above 9 for library construction ...
-
bioRxiv - Physiology 2024Quote: ... RNA concentration and quality were determined by 2100 Bioanalyzer RNA 6000 Nano Kit (Agilent) and depleted of rRNA using RiboMinus Eukaryote Kit v2 (Ambion) ...
-
bioRxiv - Neuroscience 2024Quote: ... CRISPR guide efficiency was tested in vitro using Sureguide kit (Agilent Technologies 5190-7716). Seventy-five fertilized oocytes from C57BL/6J mice were electroporated with purified 1 ng/µL Cas9 mRNA + 6 ng/µL gRNA79 ...
-
bioRxiv - Biochemistry 2024Quote: ... Point mutants were generated with the QuikChange Lightning site-directed mutagenesis kit (Agilent: 210515) according to the manufacturer protocol ...
-
bioRxiv - Plant Biology 2024Quote: ... and the RNA Nano 6000 Assay Kit forthe Bioanalyzer 2100 System (Agilent Technologies, USA). Libraries were constructed using 0.4 µg of RNA and the NEBNext ® UltraTM RNA Library Prep Kity for Illumina (NEB ...
-
bioRxiv - Genetics 2024Quote: ... Capture Hi-C libraries were generated by using the SureSelect Target Enrichment Kit (Agilent) and hybridizing the Hi-C libraries with custom probes before enrichment via Dynabeads MyOne Streptavidin Beads T1 (ThermoFisher) ...
-
bioRxiv - Genomics 2024Quote: ... We quantified libraries using the Bioanalyzer 2100 with High Sensitivity DNA kits (Agilent Technologies). We pooled two 5’ v2 gene expression technical replicates (i.e. ...
-
bioRxiv - Genomics 2024Quote: ... and Agilent Bioanalyzer 2100 with Agilent DNA 1000 Kit (Agilent Technologies, Santa Clara, CA), respectively ...
-
bioRxiv - Cell Biology 2024Quote: ... and 2 µM Antimycin A + Rotenone (Seahorse XF Cell Mito Stress Test Kit, Agilent) were added sequentially to the Agilent Seahorse XFe96 Extracellular Flux Analyzer via injection ports to determine basal and maximum respiration ...
-
bioRxiv - Neuroscience 2024Quote: ... cDNA libraries were quantified using the Agilent High Sensitivity DNA Kit (Agilent, 5067-4626) and were sequenced on an Illumina NovaSeq ...
-
bioRxiv - Immunology 2024Quote: ... with dsDNA HS (High Sensitivity) Assay Kit and size distribution was determined by Agilent High Sensitivity DNA Kit ...
-
bioRxiv - Microbiology 2024Quote: ... mutations were made by using the QuikChange II XL site-directed mutagenesis kit (Agilent) on the JN.1 construct that we previously generated1 ...
-
bioRxiv - Molecular Biology 2024Quote: ... and the mixture labelled with LowInput QuickAmp Labeling Kit One-Color (Agilent 5190-2305). For each sample ...
-
bioRxiv - Synthetic Biology 2024Quote: ... A80T and S79G/A80T were obtained with the QuickChange site-directed mutagenesis kit (Stratagene), using as template for the mutagenic reactions the plasmid pJLPM ...
-
bioRxiv - Neuroscience 2019Quote: ... with 0.3% TritonX-100 for 1 hour at room temperature and then incubated overnight at 4°C with the primary antibodies rabbit anti-Iba-1 (Wako: 019-19741, RRID:AB_839504) at 1:500 or rabbit anti-GFAP (Dako: Z0334, RRID: AB_10013382) at 1:1000 and then secondary antibodies (Alexa goat-647 anti-Rabbit ...
-
bioRxiv - Developmental Biology 2019Quote: ... incubated for 1 hour at room temperature with biotinylated goat anti-mouse or goat anti-rabbit IgG antibodies (Dako, 1:500), washed 3 times and incubated with streptavidin Cy3 (Sigma ...
-
bioRxiv - Bioengineering 2021Quote: ... Both HSV-tk and col3 used a secondary polymer-HRP anti-rabbit antibody incubation for 1 hr (1:200 Dako P0448). For col2 and endomucin staining the manufacturer’s instructions were followed (Vectastain Elite ABC kit ...
-
bioRxiv - Molecular Biology 2020Quote: ... Immunostaining was performed as described previously(45) with the following antibodies: guinea pig α-insulin (1:2000, Millipore/1:800, DAKO), mouse α-glucagon (1:2000 ...
-
bioRxiv - Neuroscience 2020Quote: ... to prevent non-specific binding was followed by a 1-hour incubation in primary antibody (pERK; 1:800) in Antibody Diluent (S0809, Agilent DAKO). Following removal of excess primary antibody with wash buffer ...
-
bioRxiv - Neuroscience 2020Quote: ... The blots were washed for 3 x 10 min in 1 x Animal-Free Blocker and incubated for 1 h at room temperature in HRP-conjugated rabbit anti-goat secondary antibody (1:1000, Dako, USA) for 1 h ...
-
bioRxiv - Developmental Biology 2021Quote: ... 2 mM Vanadyl-ribonucleoside complex NEB S1402S) mixed with 1:3000 dilution of probes and 1:900 dilution of guinea pig anti-Insulin antibody (Dako, A0564). Hybridization mix was incubated with tissues for overnight in a 30°C incubator ...
-
bioRxiv - Immunology 2022Quote: Tissue sections were then incubated with anti-PD1 (1:300, 3G8) and anti-PDL1 (1:100, 1F8) in antibody diluent (Dako, S0809) for 60 min (26) ...
-
bioRxiv - Immunology 2020Quote: ... 1 μM fluoro-carbonyl cyanide phenylhydrazone (FCCP) and 100 nM rotenone + 1 μM antimycin A (all from Agilent, Santa Clara, California) using a 96 well XF Extracellular Flux Analyzer (EFA ...
-
bioRxiv - Neuroscience 2019Quote: ... The slides were incubated overnight in primary antibody (ATRX: 1:200, H-300 Santa Cruz Biotechnology Inc; GFAP: 1:200, Agilent Technologies ...
-
bioRxiv - Cell Biology 2021Quote: ... then primary antibody (anti-SOX9 at 1/200 with 0.1% TritonX-100, ABCAM, Cat # ab185966; anti-KRT19 at 1/100, DAKO Cat # M0888) diluted in diluent (DAKO ...
-
bioRxiv - Cell Biology 2020Quote: ... and the following HRP conjugated secondary antibodies: swine anti-rabbit (1:3000 for embryo levels, 1:10000 for in vitro assays; Dako, P0399) and sheep ECL anti-mouse (1:3000 ...
-
bioRxiv - Cell Biology 2020Quote: ... HRP-conjugated antibodies were used as secondary antibodies (Swine anti-rabbit 1:3000, Goat anti-mouse 1:5000, Dako, Glostrup, Denmark). Membranes were developed with Clarity Western ECL Substrate 1:1 (Bio-Rad Laboratories ...
-
bioRxiv - Cancer Biology 2022Quote: ... dried metabolites were resuspended in 50 μL of a 1:1 DMSO to water solution and analyzed by LC-MS using a 1290 Infinity II liquid chromatography (Agilent Technologies) with a thermal autosampler set at 4°C ...
-
bioRxiv - Pathology 2024Quote: ... sections were incubated overnight at 4 ⁰C with a cocktail of the primary antibodies αSyn (clone KM51 1:500, Monosan Xtra, The Netherlands) and glial fibrillary acidic protein (GFAP, Z0334, 1:4000, DAKO, Denmark). After rinsing in PBS ...
-
bioRxiv - Immunology 2024Quote: ... The membrane was washed three times in block buffer followed by a 1 h incubation with anti-goat/-rabbit HRP-conjugated secondary antibodies (1:5000, Agilent Dako) at RT ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were then incubated in primary antibody (anti-Alk1 Rabbit Abcam #ab68703, 1:100; anti-CD31 Mouse Dako #M0823, 1:500) over-night at 4°C ...
-
bioRxiv - Neuroscience 2023Quote: ... Microglia and Astrocyte proliferation was visualized by staining the brain sections using anti Iba1 (1:2500; WAKO) and anti GFAP antibody (1:1000 Agilent technologies). When appropriate the brain sections were counterstained with haematoxylin and eosin ...
-
bioRxiv - Immunology 2023Quote: ... 1.5 µM FCCP and 1 µM Rotenone/1 µM Antimycin A from Seahorse XF Cell Mito Stress Test (103015-100, Agilent Technologies), and read using Seahorse XFe96 Analyzer (Agilent Technologies) ...
-
bioRxiv - Cancer Biology 2023Quote: Murine glioma cells (similar low passage N1IC-1/2 and p53-1/2) were seeded in PLL coated XFe24 cell culture microplates (Agilent TEchnologies) at 1.8ᵡ104 cells per well in 250μL BFP (n=10 technical replicates for the baseline experiment and n=5 technical replicates for the cysteine/methionine deprivation experiment ...
-
bioRxiv - Molecular Biology 2023Quote: ... 15 min) before incubation with either rabbit anti-mouse IgG (1.3 µg ml−1, 1% BSA in PBST; Agilent, P026002-2), goat anti-rabbit IgG (250 ng ml−1 ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... and the consumption of NADPH was followed at 30 °C and 340 nm (Δε=6.22 mM-1 cm-1) with a Cary 60 UV-Vis spectrophotometer (Agilent Technologies, Germany).
-
bioRxiv - Evolutionary Biology 2020Quote: ... The reaction was started with different concentrations of 3-butenoic acid and the consumption of NADPH was followed at 30 °C and 340 nm (Δε=6.22mM-1 cm-1) with a Cary 60 UV-Vis spectrophotometer (Agilent Technologies, Germany).
-
bioRxiv - Neuroscience 2020Quote: Mouse brains were analyzed as free-floating sections to localize immunoreactivity for GFAP (Z0334, Dako/Agilent; 1:10,000 primary, 1:10,000 secondary), Iba-1 (019-19741 ...
-
bioRxiv - Neuroscience 2020Quote: ... Membranes were incubated overnight at 4° C with primary antibodies (mouse-anti-Ataxin-3, 1:2500, clone 1H9, MAB5360, MerckMillipore, Darmstadt, Germany; rabbit-anti-Ubiquitin, 1:500, Z0458, Dako, Jena, Germany). After washing the membranes were incubated with secondary antibodies (peroxidase affinePure donkey anti-mouse IgG H+L ...
-
bioRxiv - Cell Biology 2022Quote: ... IgG1a monoclonal antibody in PBS with 1% BSA followed by a bridge step labelling with a polyclonal Rabbit Anti-Mouse Immunoglobulins (Dako, 1:50) and protein A-gold 10 nm (UMC Utrecht ...
-
bioRxiv - Immunology 2020Quote: ... followed by the incubation with horseradish peroxidase-labeled secondary antibody (1:10,000) for 1 h at room temperature (DAKO EnVision™+ System; Agilent Technologies (UK). Diaminobenzidine (DAB ...
-
bioRxiv - Immunology 2020Quote: ... 1:4000, rat anti-mouse IgG3-biotin (Becton, Dickinson, Franklin Lakes, New Jersey) 1:2000 followed by streptavidin-HRP (Dako, Glostrup, Denmark) 1:1000 incubated at 37°C for 1h.
-
bioRxiv - Cell Biology 2021Quote: ... Cells were then incubated for 1 h at room temperature with rabbit anti-glial fibrillary acidic protein (GFAP, 1:300; Dako, Carpinteria, CA), rabbit anti-oligodendrocyte specific protein (OSP ...
-
bioRxiv - Microbiology 2022Quote: ... primary antibody was produced in-house from HB 65 mouse anti-nucleoprotein and diluted 1:2500 followed by HRP-conjugated rabbit anti-mouse diluted 1:500 (Dako, Glostrup, Denmark). The titration was repeated on a different day and TCID50 titres were calculated using Reed and Muench (29).
-
bioRxiv - Neuroscience 2023Quote: ... The membrane was washed 3 times 15 minutes with TBST before a 1h incubation of horseradish peroxidase (HRP)-conjugated antibodies (P0447 goat anti-mouse IgG HRP, Dako, 1:2500 or P0399 swine anti-rabbit IgG HRP, Dako, 1:2500), and washed 3 times 15 minutes in TBST again before chemiluminescence detection ...
-
bioRxiv - Molecular Biology 2022Quote: ... Consecutive slides were incubated with anti-ATM (1:100) or anti-SMA (1:200) diluted in Dako REAL Antibody Diluent (Dako, Glostrup, Denmark). Slides were then treated with biotinylated secondary antibodies and target staining was performed with peroxidase-conjugated streptavidin and DAB chromogen (Dako REAL Detection System Peroxidase/DAB+ ...