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Citations for Agilent :
201 - 250 of 1343 citations for hsa mir 222 5p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... First strand of cDNA is synthesized using AffinityScript RT Enzyme (Agilent, 600107) according to the manufacturer’s instructions at 54? for 1 hour ...
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bioRxiv - Immunology 2024Quote: ... for 1.5h at RT and HRP-conjugated rabbit anti-rat (Agilent, P0450) for 1h at RT ...
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bioRxiv - Immunology 2023Quote: ... Ligated product was reverse-transcribed using the Affinity Script RT enzyme (Agilent) and a primer complementary to the ligated product ...
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bioRxiv - Developmental Biology 2022Quote: ... PCR products were cloned into StrataClone PCR Cloning vector (Agilent, #240205), linearized with XbaI restriction enzyme (Takara ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA was then reverse transcribed with AR17 primer and AffinityScript reverse transcriptase (Agilent). cDNA was then cleaned up by treating with ExoSAP-IT (Affymetrix ...
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bioRxiv - Synthetic Biology 2021Quote: ... Probes were labeled with the prime-it II random primer labelling kit (Agilent) using α32P -dCTP ...
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bioRxiv - Molecular Biology 2022Quote: ... a J2-4 reverse primer 5’-(GGATAGATCTCTGAGGAGACGGTGACCAGGAG-3’) and HERC II polymerase (Agilent), following the manufacturer’s recommended reaction conditions ...
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bioRxiv - Molecular Biology 2022Quote: ... Probes were synthesised using a Prime-it II Random Primer Labeling Kit (Agilent), 25 ng of target-specific DNA template ...
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bioRxiv - Genomics 2019Quote: ... The coverage and performance of primers were analysed using 2200 TapeStation instrument (Agilent) and Hi-seq 4000 ...
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bioRxiv - Neuroscience 2019Quote: ... Radioactive probes were synthetized with Prime-It II Random Primer Labeling Kit (Agilent) using 25 ng of EcoRI-digested pCR2.1-SB-3’Ube3b and α-32P-labeled dCTP (Perkin-Elmer ...
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bioRxiv - Cancer Biology 2020Quote: ... using specific primers (Table S2) and the Herculase II fusion DNA polymerase (Agilent). Previous to that ...
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bioRxiv - Genetics 2022Quote: ... The primers for the single mutagenesis experiments were designed by QuikChange software (Stratagene, San Diego ...
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bioRxiv - Plant Biology 2021Quote: ... Quantitative real-time PCR (qRT-PCR) was carried out in the Aria-MX real-time PCR system (Agilent Technologies). The transcript abundance was measured in 10 µL volume of the SYBR Green PCR Master Mix (Takara ...
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bioRxiv - Developmental Biology 2022Quote: ... a genome-wide gene expression profiling was set up using the 8×60K ArrayXS Zebrafish platform by Agilent and performed by OakLabs GmbH (Henningsdorf ...
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bioRxiv - Plant Biology 2019Quote: Samples were analysed with a 7890B GC-System coupled to a 5977B MSD quadrupole set-up from Agilent Technologies ...
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bioRxiv - Biochemistry 2021Quote: The second set of experiments was performed on Seahorse XF Cell Culture Microplates (Agilent, cat. n. 103022-100) with eight wells ...
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bioRxiv - Cancer Biology 2022Quote: ... lysed and quantified using the 480/520nm filter set in Synergy™ H1 microplate reader (Agilent BioTek Instruments).
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bioRxiv - Cell Biology 2019Quote: ... and a real-time PCR system (AriaMax Real PCR System, Agilent Technologies). A list of primers is provided in Supplementary Table 2.
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bioRxiv - Genetics 2022Quote: PCR products were cloned using StrataClone PCR Cloning Kit (Agilent, Waldbronn, Germany). Positive white colonies were randomly picked and verified with colony PCR ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The PCR reaction was performed using Brilliant PCR mix (Agilent Technologies, USA). The standard curve was drawn using serially diluted ΔintS genomic DNA ...
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bioRxiv - Neuroscience 2021Quote: ... and the PCR product was subcloned into Strataclone blunt PCR vectors (Stratagene) and subcloned further into pCM V-ONCM to generate the pCMV-SS-ONCM construct ...
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bioRxiv - Microbiology 2019Quote: ... and a 30 min incubation at RT with Envision+System HRP Rabbit (Dako). The reaction was visualized with diaminobenzidin (DAB ...
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bioRxiv - Microbiology 2019Quote: ... a 10 min incubation at room temperature (RT) with peroxidase blocking buffer (Dako) and a 30 min incubation at RT with Envision+System HRP Rabbit (Dako) ...
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bioRxiv - Neuroscience 2021Quote: ... The RT reaction was performed using a thermal cycler (Agilent Technologies, Stratagene Mx3000P) with the following parameters ...
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bioRxiv - Neuroscience 2021Quote: ... The RT reaction was performed using a thermal cycler (Agilent Technologies, Stratagene Mx3000P) with the following parameters ...
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bioRxiv - Microbiology 2021Quote: ... and a 30 min incubation at RT with Envision+System HRP Rabbit (Agilent). The reaction was visualized with diaminobenzidin (DAB ...
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bioRxiv - Biochemistry 2023Quote: cDNA was used for RT-qPCR with miRNA QPCR Master Mix (Agilent Technologies). Universal reverse primers (Agilent Technologies ...
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bioRxiv - Microbiology 2023Quote: ... and a 30 min incubation at RT with Envision+System HRP Rabbit (Agilent). The reaction was visualized with diaminobenzidin (DAB ...
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bioRxiv - Microbiology 2021Quote: ... primer P3 (Table S2) was used in the QuikChange Multisite-directed mutagenesis kit (Agilent), and the resulting plasmid was transformed into XL10 Gold ultracompetent Escherichia coli cells ...
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bioRxiv - Genomics 2020Quote: ... were subjected to site-directed mutagenesis with primers EF77 and EF78 using PfuUltraII (Agilent) in 50μl reactions ...
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bioRxiv - Cancer Biology 2020Quote: ... and specific primers on a Stratagene Mx3005P instrument (Agilent Technology, Santa Clara, CA, USA). Primer sequences are listed in Supplementary Table 3 ...
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bioRxiv - Cancer Biology 2020Quote: ... and specific primers on a Stratagene Mx3005P instrument (Agilent Technology, Santa Clara, CA, USA). Primer sequences are listed in Supplementary Table 2 ...
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bioRxiv - Biochemistry 2021Quote: ... Oligonucleotides sequences designed with Quik Change Primer Design program (Agilent Technologies, Santa Clara, USA), are shown in table 6 ...
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bioRxiv - Immunology 2023Quote: ... Oligos introducing site-specific mutations were designed with the QuikChange Primer Design Tool (Agilent), synthesized and purified via standard desalting by Integrated DNA Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... Southern blot probes were radiolabeled using the Prime-it Random Primer Labeling Kit (Agilent) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and specific primers on a Stratagene Mx3005P Instrument (Agilent Technology, Santa Clara, CA, USA). The sequence of all human primers is shown in Supplementary Table 1 ...
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bioRxiv - Plant Biology 2021Quote: ... Each sample set was run together with the external standard (2-AA labeled dextran ladder, Glyko Prozyme, Hayward, CA). In each chromatogram 15 individual peaks were identified and peak assignments were made basing on glucose units (GU) ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... A transfer line set at 250 °C led to a 5977A mass spectrometer (Agilent Technologies Inc., Santa Clara, CA) with a solvent delay of 5 min ...
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bioRxiv - Genomics 2020Quote: ... The coverslips were incubated with 10 μL mixture of a custom probe set targeting a selected DNA locus (Agilent) and SureFISH Hybridization Buffer (Agilent ...
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bioRxiv - Microbiology 2020Quote: ... Reactions were set up in a 25 µl total volume with 12.5 µl Brilliant SYBR Green qPCR Master Mix (Agilent), 0.4 µM of each primer and 5 µl of prediluted DNA ...
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bioRxiv - Genomics 2020Quote: ... libraries were balanced optimally for whole exome sequencing using the SureSelect Canine All Exon V2 bait set from Agilent. Library sequencing was performed on the Illumina Hiseq 2500 platform.
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bioRxiv - Immunology 2022Quote: ... The final set of nucleotide sequences was collapsed at 100% nucleotide sequence identity (n = 238,068) and then ordered from Agilent Technologies.
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bioRxiv - Cancer Biology 2019Quote: ... Quantitative PCR was performed by using QuantiNova kit on Mx3500P PCR machine (Stratagene). The following oligonucleotide primers were used to detect target gene expression ...
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bioRxiv - Plant Biology 2021Quote: ... Chimeric PCR products were subcloned before sequencing using StrataClone PCR cloning kits (Agilent).
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bioRxiv - Immunology 2021Quote: ... Real-time PCR was performed on an AriaMx Real-Time PCR system (Agilent) using primer/probes from ThermoFisher targeting exon 5 of the Ido1 gene (Mm00492590_m1 ...
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bioRxiv - Cell Biology 2022Quote: ... Quantitative PCR was performed using the AriaMx Real-Time PCR System (Agilent Technologies). Expression data was normalized to the housekeeping gene ribosomal protein lateral stalk subunit P0 (Rplp0) ...
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bioRxiv - Genomics 2021Quote: ... PCR amplifications were performed in an Mx3005P Real-Time PCR System (Agilent, USA) using the following cycling conditions ...
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bioRxiv - Cell Biology 2023Quote: ... The PCR assay was conducted using the AriaMx qRT-PCR detection system (Agilent). Gene expression values were normalized to the GAPDH housekeeping gene to calculate the ΔCt value.
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bioRxiv - Molecular Biology 2019Quote: ... RT-qPCR analyses were performed using Brilliant III Ultra-Fast SYBR® Green (Agilent) after DNase treatment using Turbo DNase (AMBION) ...
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bioRxiv - Bioengineering 2021Quote: ... were applied for 30 mins at RT and samples were exposed DAB substrate (Dako) for 5 mins ...