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Citations for Agilent :
451 - 500 of 1407 citations for hsa mir 222 5p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... and AriaMX Real-Time PCR machine (Agilent Technologies). Primers used for qPCR are listed in Table S4.
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bioRxiv - Microbiology 2022Quote: ... on a Mx3005P Real-Time PCR System (Agilent). The 20µl reaction was as follows ...
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bioRxiv - Microbiology 2022Quote: ... on a Mx3005P Real-Time PCR System (Agilent). The primers used for cDNA amplification were the following ...
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bioRxiv - Biochemistry 2023Quote: ... and run on AriaMx Real-time PCR (Agilent). The sequences of the primers that were used for the qPCR were as follows ...
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bioRxiv - Microbiology 2023Quote: ... PCRs were performed with Stratagene Mx3005P (Agilent Technologies) with a 0.04 threshold ...
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bioRxiv - Developmental Biology 2023Quote: ... 4C PCR library efficiencies were confirmed by Agilent Bioanalyzer and libraries were sequenced on a HiSeq4000 in PE150 mode by the MSKCC Genomics Core Facility ...
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bioRxiv - Microbiology 2023Quote: ... using a AriaMx Real-Time PCR system (Agilent).
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bioRxiv - Plant Biology 2023Quote: ... The PCR reactions used Pfu Turbo Cx (Agilent), dNTP (Takara Bio) ...
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bioRxiv - Cell Biology 2024Quote: ... and an AriaMX Real-Time PCR machine (Agilent). Primers used for RTqPCR are listed in Table_2.
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bioRxiv - Microbiology 2023Quote: ... prior to PCR analysis following manufacturer’s guidelines (Agilent). Gene-specific TaqMan probes (Applied Biosystems ...
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bioRxiv - Neuroscience 2023Quote: ... The Aria Mx Real-Time PCR system (Agilent) was used and the data were extracted from the AriaMx PC software.
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bioRxiv - Cancer Biology 2023Quote: ... The PCR samples were also analyzed by Agilent 2100 Bioanalyzer system (Agilent Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... using the StrataClone PCR Cloning Kit (#240205, Agilent). The bi-allelic sequencing was performed with the M13 reverse primer ...
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bioRxiv - Systems Biology 2023Quote: ... on a AriaMx Real-time PCR System (Agilent) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... mutations were corrected by PCR-based mutagenesis (Stratagene). Secondly ...
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bioRxiv - Molecular Biology 2024Quote: ... An AriaMx Real-time PCR System (Agilent Technologies) was used for quantification of RNA levels and the X0 method was used for calculations of relative RNA levels (Thomsen et al. ...
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bioRxiv - Biophysics 2021Quote: Room-temperature (RT) absorption spectra of cells were acquired on a Varian Cary 4000 UV–Vis-spectrophotometer (Agilent technologies) equipped with an integrating diffuse reflectance sphere (DRA-CA-50 ...
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bioRxiv - Neuroscience 2020Quote: ... slides were washed again and incubated for 30 min at RT with Envision+ HRP labelled polymer anti-mouse (DAKO). Subsequently ...
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bioRxiv - Microbiology 2023Quote: ... pLAIΔEnvGFP.Gag-LUC RT D185E and INT D116N were generated by site-directed mutagenesis using Pfu Turbo DNA polymerase (Agilent) and the following primers:
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bioRxiv - Neuroscience 2023Quote: ... cDNA of the first RT reaction as well as the final library was QC’ed via High Sensitivity DNA chip bioanalyzer (Agilent).
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bioRxiv - Biophysics 2021Quote: ... with help of complementary primers containing mutations (Eurofins MWG Operon, Germany) using the QuikChange protocol for site-directed mutagenesis (Stratagene). Sanger sequencing either outsourced to SourceBioscience (Nottingham ...
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bioRxiv - Biochemistry 2021Quote: ... The RNA was sheared and reverse transcribed with N6 random primers to obtain cDNA library and then qualified by Agilent 2100 Bioanalyzer ...
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bioRxiv - Cell Biology 2019Quote: ... Point mutations were made with complementary primers (listed below) in pTRCHisA-padi2 vectors using QuikChange II Site-Directed Mutagenesis Kit (Agilent).
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bioRxiv - Biochemistry 2022Quote: ... The H335A mutation that renders PLC-γ1 catalytically inactive was introduced into the transfer vector encoding wild-type PLC-γ1 (residues 21-1215) or PLC-γ1 (D1165H) using standard primer-mediated mutagenesis (Agilent Technologies ...
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bioRxiv - Genetics 2019Quote: ... Each of the 210 RHO variants was created via site-directed mutagenesis using a one-primer modification to the QuikChange II protocol from Agilent.((Braman ...
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bioRxiv - Molecular Biology 2019Quote: ... the membranes were incubated with [α-32P]dCTP radioactively labelled DNA probes (Prime-IT RmT Random Primer Labelling Kit, Stratagene) overnight at 65°C ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the CMV enhancer was amplified with the primers P322 and P323 using the phrGFPII-1 plasmid (Agilent Technologies, Santa Clara) as template ...
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bioRxiv - Biochemistry 2020Quote: ... Selected mRNAs were purified and reverse-transcripted into single-chain DNA with the primer 5’-CTTCAGTTGCCGCTTTCTTTCTTG-3’ using a reverse transcriptase (Cat 200436, Agilent). The resulting cDNA library was purified using a DNA purification kit (Cat A740609.25 ...
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bioRxiv - Immunology 2021Quote: ... Product was amplified and barcoded with adaptor specific primers and the quality of the resulting libraries were determined by Agilent Bioanalyzer ...
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bioRxiv - Systems Biology 2022Quote: ... to remove larger DNA fragments and primer dimers and quality was assessed by Bioanalyzer (Agilent 2100, High Sensitivity DNA kit). Samples were pooled according to indexes for Illumina sequencing.
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bioRxiv - Molecular Biology 2020Quote: ... were introduced using RE specific primers (Table 1 and Table 2 in supplementary material) by QuickChange Multi site-directed mutagenesis kit (Agilent) according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2019Quote: ... Input samples were dephosphorylated and 3’-linker ligated to a custom oligonucleotide primer and all samples (IPs and SMInputs) reverse transcribed using AffinityScript reverse transcriptase (Agilent). After treatment with ExoSAP-IT (Affymetrix ...
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bioRxiv - Cancer Biology 2019Quote: ... pGL3-DARPP-32 plasmids were used as templates in PCR reactions to synthesize plasmids containing mutated ASCL1 binding sites by annealing and amplifying site-directed mutagenesis primers using QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent). Digestion with Dpn I enzyme was carried out to eliminate methylated parental plasmid strands ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Transferred DNA was UV crosslinked to the membrane and hybridized with a 32P-labeled (Prime-It II Random Primer Labeling Kit, Agilent) Lpar1 probe with sequence external to the 5’ end of the targeting vector ...
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bioRxiv - Developmental Biology 2019Quote: ... pBMN-HIPK4-Y175F-3xFLAG-IRES-mCherry was later generated by site-directed mutagenesis of the wild type construct using the primers 5’-CGCTATGTGAAGGAGCCTTTCATCCAGTCCCGCTTC TAC-3’ and 5’-GTAGAA GCGGGACTGGATGAAAGGCTCCTTCACATAGCG-3’ and PfuUltra II Fusion polymerase (Agilent).
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bioRxiv - Cancer Biology 2021Quote: ... pCMVHA hEZH2 and V5-EZH2 vector was used to generate EZH2-H689A mutant vector using the mutagenesis primers listed in Table S1 with QuikChange II site-directed mutagenesis kit (Agilent) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... USP47 catalytic domain was mutated by changing cysteine 177 into alanine (C177A) using the sense primer (5’-gtttgcaaaaggctattcaaataggcagtcattgcttggtttactagtcc-3’) using Quickchange Lightning Site-directed Mutagenesis Kit from Agilent technologies ...
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bioRxiv - Microbiology 2024Quote: ... The E50A variant was further obtained by site-directed mutagenesis using an adapted protocol from the Stratagene “QuickChange” with only one mutator primer carrying the modified sequence and with both the Pfu turbo polymerase (Stratagene) and the Taq ligase (New England Biolabs ...
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bioRxiv - Molecular Biology 2024Quote: ... The quality and yield of fully-barcoded DNA primers was evaluated with total RNA Pico Assay (Agilent Technologies, 5067-1513) and by fluorescent in situ hybridization (FISH ...
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bioRxiv - Genetics 2024Quote: Membranes were hybridized with “probe 4” for the HIS4::LEU2 hotspot 100 which was 32P-dCTP labelled using the Prime-It II Random Primer Labeling Kit (Stratagene). Following hybridization ...
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bioRxiv - Molecular Biology 2022Quote: ... Point mutations in the repM gene were generated using specific primers and a QuikChange Site-Directed Mutagenesis kit according to the protocol supplied by the manufacturer (Stratagene). All oligonucleotide primers used in this study are listed in Supplementary Table S2.
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bioRxiv - Microbiology 2023Quote: ... along with gene-specific primers listed in Supplemental Table 3 and qPCR was performed in technical triplicate on a StepOnePlus (Stratagene). Ct values of target genes were normalized to β-actin and relative gene expression levels between conditions were calculated via the ΔΔCt method ...
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bioRxiv - Microbiology 2023Quote: ... and a barcoding primer (unique for each sample to allow for demultiplexing of reads) with the Easy A cloning kit (Agilent). A 200–400 bp product was gel-purified and sequenced on an Illumina NextSeq Platform (in lab ...
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bioRxiv - Plant Biology 2023Quote: ... the first-strand complementary DNA (cDNA) was synthesized from 1 μg of RNA using oligo (dT) primers with Reverse Transcriptase (Agilent). Quantitative reverse transcription polymerase chain reaction (qRT-PCR ...
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bioRxiv - Neuroscience 2023Quote: ... pooled libraries were additionally purified with AMPure beads (ratio 1:1) to remove contaminating primer dimers and quantified using Qubit and the Bioanalyzer High-Sensitivity DNA kit (Agilent). 50-bp paired-end deep sequencing was carried out on HiSeq 4000 (Illumina).
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bioRxiv - Cancer Biology 2023Quote: ... Gene-specific primers were used (Table S1) together with Brilliant III Ultra-Fast SYBR Green QPCR Master Mix (Agilent Technologies), following the manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2023Quote: CDS sequences from PhDEF and PhGLO1 were amplified from Petunia x hybrida R27 inflorescence cDNAs with primers MLY2382/MLY2383 and MLY2384/2385 respectively (Supplementary Table 1) and cloned into the in vitro translation vector pSPUTK (Stratagene) by NcoI/XbaI restriction ...
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bioRxiv - Microbiology 2023Quote: ... primer annealing and extension at 58°C for 30 s followed by dissociation step in a thermal cycler (Stratagene Mx3005P). Finally ...
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bioRxiv - Biochemistry 2023Quote: ... The NopD point mutants constructs were generated by different primers and were created by the QuickChange site-directed mutagenesis kit (Stratagene). All primers are shown in the Supplementary Table1 ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were then assessed for appropriate amplicon size and complete elimination of free primer peaks using the High Sensitivity ScreenTape Assay on the Tape Station2200 (Agilent) and quantified using the qPCR-based quantification in order to ensure only NGS-compatible amplicon was quantified using the Library Quant ROX Low Kit (Kapa Biosystems ...