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Citations for Agilent :
201 - 250 of 8452 citations for Mouse T cell receptor alpha chain C region TCRA ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Cassettes and the template plasmid pM07704 were amplified by polymerase chain reaction (PCR) with Herculase II DNA polymerase (Stratagene). Marker-exchange plasmids were generated by ligation of the PCR products in α-select cells (Bioline ...
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bioRxiv - Neuroscience 2020Quote: ... nucleotides: 907-1550) was amplified by the use of polymerase chain reaction (PCR) and cloned into pBluescript KS+ (Stratagene) via ClaI-EcoRI sites ...
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bioRxiv - Microbiology 2023Quote: ... Real-time Polymerase Chain Reaction (qPCR) was conducted in a Stragene Mx3005P real-time PCR system (Agilent Technologies®) to analyze the relative expression of the cytokine genes Interleukin 2 (IL-2) ...
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bioRxiv - Physiology 2023Quote: ... Hepatic portal vein bile acids and short chain fatty acids were acquired using an Agilent 1290 series HPLC (Agilent,) with an Acquity C18 BEH (2.1mm x 50mm ...
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bioRxiv - Immunology 2023Quote: ... Cell Mito Stress Test Kit (Agilent Technologies). Final inhibitor concentrations in the wells were 1.5 μM oligomycin ...
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bioRxiv - Microbiology 2021Quote: ... cells were incubated with HRP-conjugated rabbit anti-mouse antibody (DaKo, P0161) diluted 1:5000 in blocking buffer for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were incubated with mouse anti-BrdU (DAKO clone BU20A 1:40) for 1h ...
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bioRxiv - Neuroscience 2019Quote: ... and SNAREQ (SNAP25 K40A/D51A/E52A/E55A/D166A) were generated in the above described Syt1 and t-SNARE background respectively using the QuickChange mutagenesis kit (Agilent Technologies, Santa Clara, CA). All proteins were expressed and purified as described elsewhere (Mahal et al ...
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bioRxiv - Microbiology 2024Quote: ... however with GFP Mouse mAb as a primary antibody diluted 1:1000 in PBST (Cell Signalling, 4B10) and Anti-mouse peroxidase (Dako, P0447) as a secondary antibody diluted 1:5000 ...
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bioRxiv - Cancer Biology 2021Quote: ... rabbit/mouse following manufacturer’s instructions (Detection Kit #K5005, Agilent Technologies, Santa Clara, CA, USA). Immunostained tissue sections were counterstained with hematoxylin solution according to Mayer (T865.1 ...
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bioRxiv - Immunology 2020Quote: Whole-exome sequencing libraries were constructed using the SureSelect Mouse all Exon Kit (Agilent). RNA sequencing libraries were constructed using the TrueSeq Stranded mRNA Sample Prep kit (Illumina) ...
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bioRxiv - Cancer Biology 2022Quote: ... the sections were incubated in HRP rabbit/mouse secondary antibody (DAKO Real EnVision kit) for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2022Quote: RNA was extracted from mouse lungs using the Absolutely Total RNA Purification Kit (Agilent). RNA extraction from cell culture experiments were performed using the Qiagen RNeasy kit (Qiagen) ...
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bioRxiv - Cell Biology 2023Quote: Bsp1 proteins were expressed overnight at 20°C in BL21 pLysS Gold competent cells (Agilent) using autoinduction LB medium (Formedium) ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were then washed with PBS and stored at 4°C in Mounting medium (Dako).
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bioRxiv - Bioengineering 2019Quote: ... followed by overnight staining with 1:300 dilutions of rat-anti-C-peptide (DSHB; GN-ID4-S) and mouse-anti-CD31 (Dako; M082329-2) primary antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... The plates were then incubated at 37°C for 48 hours then analyzed by flow cytometry and ELISA or monitored for target cell death using xCelligence eSight (Agilent Technologies, Inc., Santa Clara, CA).
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bioRxiv - Physiology 2024Quote: Fuel preference of CRISPR/Cas9 Pparγ edited cells (γY4KO) and mock-edited cells (C) was measured using Seahorse Mito-Fuel Flex test (Agilent Technologies), in assay medium as above and according to the manufacturer protocol ...
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bioRxiv - Immunology 2020Quote: ... The processed T cell samples were pooled and quantified relatively utilizing capillary electrophoresis time-of-flight mass spectrometry (CE-TOF-MS, Agilent Technologies, Waldbronn, Germany) with analysis performed by Human Metabolome Technologies ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... cells were harvested from the T-75 flasks and seeded into a Seahorse 96-well XF Cell Culture microplate (Agilent Seahorse Bioscience, CA, USA) in 80 µL of the culture medium at a density of 20,000/well ...
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bioRxiv - Molecular Biology 2023Quote: ... The hybridization was performed according to the manufacturer instructions on 4×180K mouse microarrays (SurePrint G3 Mouse CGH Microarray Kit, 4x180K, AGILENT Technologies, reference genome: mm9). Microarrays were scanned with an Agilent High-Resolution C Scanner using a resolution of 3 µm and the autofocus option ...
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bioRxiv - Developmental Biology 2019Quote: ... RT-PCR was performed to amplify the dazl coding region using Easy-A High Fidelity Taq polymerase (600400, Agilent) (primers in Supplemental Table 1) ...
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bioRxiv - Plant Biology 2021Quote: ... The genomic SPL7 (AT5G18830.1) coding region (translational start to stop codon) was PCR-amplified and cloned into the pBluescript SK+ vector (Stratagene/Agilent Technologies ...
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bioRxiv - Genomics 2021Quote: PCR was performed from each DNA sample for four regions (ORF21, ORF34, ORF46, and ORF18) using Herculase II (Agilent). Each region was then PCR purified (GeneJet ...
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bioRxiv - Genetics 2022Quote: ... for fragments corresponding to a 10.3-kb genomic region (coordinates 66,234 – 76,516) of the BL21AI genome [14] using custom-designed biotinylated ssDNA probes (Agilent SureSelect) and streptavidin-bead purification ...
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bioRxiv - Biochemistry 2020Quote: ... Selected mRNAs were purified and reverse-transcripted into single-chain DNA with the primer 5’-CTTCAGTTGCCGCTTTCTTTCTTG-3’ using a reverse transcriptase (Cat 200436, Agilent). The resulting cDNA library was purified using a DNA purification kit (Cat A740609.25 ...
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bioRxiv - Biophysics 2022Quote: All of mutations in this study were made by using overlap-extension polymerase chain reaction (PCR) with Pfu polymerase (Stratagene) on the template of the mbr5 splice variant of mslo1 (Uniprot ID ...
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bioRxiv - Immunology 2020Quote: ... and bound mAbs were detected with rabbit anti-human IgG gamma chain-specific/HRP (Agilent: cat. P0214, 1:15,000 dilution).
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bioRxiv - Cell Biology 2023Quote: ... was purchased from Jackson ImmunoResearch Laboratories and was used for Western blotting. Affinity-purified rabbit polyclonal anti-human lambda-light chain (cat. A019302-2) was purchased from Dako and was used for Western blotting ...
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Development of a genetically-encoded sensor for probing endogenous nociceptin opioid peptide releasebioRxiv - Neuroscience 2023Quote: ... site directed mutagenesis and Circular Polymerase Extension Cloning was performed by polymerase chain reaction with custom designed primers using a Pfu-Ultra II fusion High Fidelity DNA Polymerase (Agilent). Sanger sequencing (Microsynth ...
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bioRxiv - Neuroscience 2021Quote: The MRI experiments were performed on a 9.4 T Varian (Agilent) Direct Drive MRI system (Varian Medical Systems Inc. ...
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bioRxiv - Microbiology 2023Quote: ... were identified using a modified t-test (GeneSpring 14.5; Agilent Technologies) with Benjamini and Hochberg multiple testing correction and defined as those with p<1×10-5 and minimum fold change of 2.0.
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bioRxiv - Microbiology 2023Quote: ... were identified using a modified t-test (GeneSpring 14.5; Agilent Technologies) with Benjamini and Hochberg multiple testing correction and defined as those with p<5×10-5 and minimum fold change of 2.0 ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were incubated with polyclonal goat anti-mouse FITC (DAKO F0479 1:20). Finally ...
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bioRxiv - Neuroscience 2022Quote: ... Libraries were prepared according to Illumina’s instructions and SureSelectXT Mouse All Exon enrichment kits (Agilent) were used ...
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bioRxiv - Cancer Biology 2023Quote: ... exome capture was performed using the Agilent SureSelect Mouse All Exome QXT capture kit (Agilent). Briefly ...
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bioRxiv - Cancer Biology 2021Quote: ... The XF Cell glycolysis Test Kit (Agilent, 103020), was used for the assay ...
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bioRxiv - Molecular Biology 2020Quote: ... Seahorse XF Cell Mito Stress kit (Agilent Technologies) was used according to manufacturer’s instructions and modified for zebrafish purposes as described in previous publications [80–82] ...
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bioRxiv - Cancer Biology 2021Quote: ... Seahorse Cell Mito Stress Test Kit (Agilent Technologies) was used to measure mitochondrial OxPHOS ...
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bioRxiv - Cell Biology 2022Quote: ... using the Cell MitoStress Test Kit (Seahorse Bioscience), according to manufacturer instructions ...
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bioRxiv - Physiology 2021Quote: Oxygen consumption was measured at 37°C from isolated mouse and human islets after overnight recovery using a Seahorse XF24 analyzer (Agilent, Santa Clara, CA). Islets were seeded at a density of 75 islets/well ...
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bioRxiv - Microbiology 2023Quote: ... cells were grown with agitation at 37°C in an imaging multimode reader (Cytation 5, Agilent). Cell growth was monitored every 5 min by measuring absorbance at 600 nm ...
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bioRxiv - Cancer Biology 2023Quote: Quality and quantification of the Hi-C libraries was assessed using the 2100 Bioanalyzer (Agilent, DNA 7500 kit). An equimolar pool of the different samples was sequenced on the Illumina NextSeq 550 System in a 75bp paired-end setup ...
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bioRxiv - Genomics 2023Quote: Quality and quantification of the Hi-C libraries was assessed using the 2100 Bioanalyzer (Agilent, DNA 7500 kit). Four biological replicates have been pooled in an equimolar manner and subjected to sequencing using the Illumina NextSeq 550 System in a 75bp paired-end setup ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were heated from 25 °C to 96 °C at 1 °C/min using an Mx3005P qPCR machine (Agilent Technologies). The dye was excited at 492 nm ...
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bioRxiv - Biochemistry 2019Quote: The genomic DNA template was amplified by the polymerase chain reaction (PCR) using Pfu Ultra High-Fidelity DNA polymerase (Agilent Technologies). Each 50 μL reaction consisted of genomic DNA (~12 ng) ...
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bioRxiv - Cell Biology 2019Quote: ... Quantitative real-time polymerase chain reaction (qPCR) was performed in triplicate using master mix (Brilliant III Ultra-Fast SYBR Green QPCR Master Mix, Agilent Technologies) and a real-time PCR system (AriaMax Real PCR System ...
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bioRxiv - Bioengineering 2021Quote: ... Mutations were cloned into the expression vector by polymerase chain reaction (PCR) using a PFUultra II Hotstart PCR Master Mix (Agilent Technologies). The obtained PCR products were subsequently treated with DPNI (New England Biolabs ...
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bioRxiv - Cell Biology 2022Quote: ... full-length cDNAs of the zebrafish banp gene were amplified with Polymerase Chain Reaction (PCR) and sub-cloned into pBluescript II SK(+) vectors (Stratagene/Agilent Technologies). cDNA fragments were amplified from mRNA with PCR using the primers for cenpt ...
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bioRxiv - Cell Biology 2019Quote: ... and indels were determined by amplification of the sgRNA target site by polymerase chain reaction using Herculase II Fusion DNA Polymerase (Agilent biotechnologies) and Sanger sequencing ...