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Citations for Agilent :
51 - 100 of 8452 citations for Mouse T cell receptor alpha chain C region TCRA ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... washed with TBS-T and next incubated with HRP-conjugated anti-mouse IgG (1:2000, DAKO, P0447) or anti-rabbit IgG (1:2000 ...
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bioRxiv - Immunology 2022Quote: ... except that rabbit-anti-mouse IgG-HRP (DAKO; 1:10.000 diluted in TBS-T with 1% BSA) was used as secondary antibody.
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bioRxiv - Immunology 2023Quote: ... and Polyclonal Goat Anti-Mouse Immunoglobulins/HRP (Dako p0447; 1:5000 in 1% BSA and TBS-T)) and incubated for 1 h at room temperature with gentle shaking ...
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bioRxiv - Molecular Biology 2024Quote: ... Variant calling was restricted to regions covered by Agilent v6 kit ...
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bioRxiv - Biochemistry 2021Quote: ... AccuScript high-fidelity first-strand cDNA synthesis kit for reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) was procured from Agilent.
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bioRxiv - Immunology 2023Quote: ... The variable heavy chain sequence of CH42 was subcloned into a plasmid coded with an IgG1 heavy chain backbone containing the N297A 29 mutation via QuikChange Site Directed Mutagenesis kit (Agilent). Antibodies were expressed through co-transfection of heavy and light chain plasmids in Expi293 HEK cells (Thermo Fisher ...
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bioRxiv - Immunology 2024Quote: A total of 10 µg/ml rHA were coated on an ELISA plate at 4 °C ON and afterwards incubated with rabbit anti-HA antibody (1:2000 dilution) (Dako, A0001) 2h at RT ...
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bioRxiv - Cell Biology 2019Quote: RNA was extracted from mouse embryonic stem cells using the Absolutely RNA microprep kit (Agilent). 1 µg of RNA was pre-incubated with 4.5 µg of (PR)20 or water for 10 min at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... IgA heavy chain (rabbit anti-human, Dako, A0262 1:1000) and secretory component/pIgR (goat anti-human ...
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bioRxiv - Microbiology 2020Quote: ... Polymerase chain reaction (PCR) was performed using Pfu Turbo (Stratagene).
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bioRxiv - Cell Biology 2019Quote: ... progesterone receptor (CST 8757, 1:25; Dako A0098, 1:20), estrogen receptor (Santa Cruz sc-542 ...
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bioRxiv - Genetics 2024Quote: ... The SCN5A mutations E171Q (Ensembl transcript ENST00000333535 c.511G>C) and R222Q (c.665G>A) were introduced with a QuikChange Multi Lightning kit (Agilent) with primers 5’ttcaccgccatttacacctttCagtctctggtca (E171Q ...
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bioRxiv - Evolutionary Biology 2020Quote: ... RNA probes were synthetized by cloning the DNA amplified region in the Strataclone PCR Cloning Kit (240205-5, Agilent Technologies) (hoxd12a ...
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bioRxiv - Biophysics 2021Quote: The triplex-forming regions were incorporated into the parent plasmids by site-directed mutagenesis using the QuikChange Site-Directed Mutagenesis Kit (Stratagene) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... Exome regions were captured by an Agilent SureSelect Human All Exon V4 Kit and V5+ LincRNA (Agilent Technologies, CA, USA). Sequencing was performed on an Illumina HiSeq 2000 instrument (Illumina ...
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bioRxiv - Cell Biology 2022Quote: Mutations to delete the αCT11 region (RPRPDDLEI) of GJA1-20k and GJA1-WT was performed using the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... we changed three amino acids within the Fc region by using the QuikChange II site directed mutagenesis kits (Agilent, #200523), introducing M257Y ...
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bioRxiv - Developmental Biology 2022Quote: ... The coding exons and flanking intronic regions were enriched with the SureSelectXT Low Input All Exon v7 kit (Agilent Technologies) followed by dual index ...
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bioRxiv - Genetics 2021Quote: ... Partition barcoded libraries were then prepared based on the Chromium Exome Solution (10X Genomics) and the exome target regions was enriched by SureSelect Human All Exon V5 Kit (Agilent) according to the protocols ...
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bioRxiv - Biochemistry 2020Quote: The supernatants of the quenched in vitro OMT reactions and fermentation samples were analyzed by reversed-phase HPLC (instrument: Agilent 1100; autosampler: HiP sampler G1367A, T=4°C, 10 μL injection; column: Agilent Zorbax Eclipse XDB-C18 80Å ...
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bioRxiv - Biochemistry 2023Quote: ... Shimadzu LC-10AT; autosampler: HiP sampler G1367A, T = 4°C, 10 µL injection; flow rate: 1 mL/min; column: Agilent Zorbax Eclipse XDB-C18 80Å ...
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bioRxiv - Molecular Biology 2022Quote: ... washed with TBS-T and next incubated with secondary HRP-conjugated anti-mouse IgG (1:2,000, DAKO, P0447) for 1 hour at room temperature and developed using the ECL detection kit (Perkin Elmer ...
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bioRxiv - Physiology 2020Quote: ... the sections were washed in TBS-T and incubated with HRP-conjugated anti-mouse secondary antibody (Dako, K4000). The sections were stained brown by incubation of 3,3′-diaminobenzidine (DAB) ...
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bioRxiv - Neuroscience 2024Quote: ... PVDF membranes were washed thrice in TBS-T before being incubated with goat anti-mouse-HRP (Dako, P0447) or goat anti-rabbit-HRP (Dako ...
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bioRxiv - Physiology 2021Quote: Two truncated recombinant fibrinogen α-chain variants (α220 and α390) were produced using QuikChange II Site-Directed Mutagenesis Kits from Agilent Technologies (Stockport ...
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bioRxiv - Immunology 2020Quote: ... The RM19R Fab heavy chain plasmid was made introducing two stop codons following residue D234 in the RM19R IgG1 heavy chain vector using the QuikChange® Lightning Site-Directed Mutagenesis kit (Agilent). The RM19R Fab was expressed in HEK293F cells (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... Fab expression vectors were made by introducing two stop codons following residue D234 (Kabat numbering 57) in the IgG heavy chain vectors using the QuikChange® Lightning Site-Directed Mutagenesis kit (Agilent). Sequences were verified by Sanger sequencing (Genewiz).
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bioRxiv - Bioengineering 2020Quote: ... or anti-alpha Fetoprotein (AFP) (DAKO, Cat. # A0008; 1:300). Cardiomyocytes were immunostained with anti-α-Actinin (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... a monoclonal mouse antibody against the intracellular tail of human HLA-DR α chain was used (Clone TAL.1B5, # M0746, Agilent, Santa Clara, CA, USA). Detection of MHC-I on the surface of cells was performed using a monoclonal mouse antibody against devil beta-2-microglobulin (B2M ...
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bioRxiv - Genomics 2019Quote: We analyzed somatic mutations in 1642 miRNA gene regions (Supplementary Table S1) including 1600 regions covered by the probes used for exome enrichment (SureSelect, Agilent, Santa Clara, CA, USA) and 42 regions co-captured during library enrichment but not directly covered by the SureSelect probes ...
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bioRxiv - Immunology 2023Quote: was measured in CAR-T cells using the Seahorse XFe96 Instrument (Seahorse Bioscience). 200,000 cells per well were seeded on poly-D-lysine-coated plates on the same day of the experiment according to manufacturer instructions ...
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bioRxiv - Pathology 2023Quote: ... Sections from the first level (main experiment) were stained for smooth muscle alpha-2 actin (ACTA2) and galectin 3 (LGALS3) using mouse monoclonal anti-ACTA2 (Dako, cat. no. M0851, 1:100) after blocking with Fab fragment (Jackson ImmunoResearch ...
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative polymerase chain reaction (qPCR) was performed using SureCycler 8800 (Agilent) and AriaMx Real-Time PCR System (Agilent) ...
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bioRxiv - Microbiology 2020Quote: Polymerase chain reactions were performed using Pfu Ultra II polymerase (Agilent) or Clontech Hifi polymerase (Takara) ...
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bioRxiv - Immunology 2023Quote: Antibody heavy chain plasmids were mutated using the QuikChange Lightning (Agilent) kit ...
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bioRxiv - Cell Biology 2021Quote: ... fixed paraffin-embedded and analysis of alpha and beta cell proliferation was performed in sections immunolabeled with anti-insulin (A5064, Agilent, Santa Clara ...
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bioRxiv - Developmental Biology 2021Quote: ... along with 60 ng/well of pERV3 receptor plasmid (Agilent Technologies) containing a modified ecdysone receptor (VgEcR ...
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bioRxiv - Plant Biology 2019Quote: ... The probes were prepared using Prime-It® Rm T Random Primer Labeling kit (Stratagene). The probe for AtMIOX4:pBIB-Kan was amplified with npt-II forward (5’-AGA GGC TAT TCG GCT ATG AC-3’ ...
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bioRxiv - Evolutionary Biology 2020Quote: ... or with minor N-terminal truncations to exclude the transmembrane regions (Sphaeroforma arctica: amino acids 21-316; Auxenochlorella protothecoides: amino acids 21-327) in Arctic Express DE competent cells (Agilent). At the N-terminus ...
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bioRxiv - Cell Biology 2021Quote: ... anti-smooth muscle alpha-actin (α-SMA - Dako GA611, 1:100), anti-ACE2 (Merck SAB3500346 ...
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bioRxiv - Cancer Biology 2020Quote: ... T-ALL cells were resuspended in Seahorse XF RPMI Medium (103576-100, Agilent Technologies) supplemented with 10 mM glucose (1003577-100 ...
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bioRxiv - Cancer Biology 2022Quote: ... T-ALL cells were resuspended in Seahorse XF RPMI Medium (Agilent Technologies, 103576-100) supplemented with 10 mM glucose (Agilent Technologies ...
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bioRxiv - Biochemistry 2019Quote: ... the gene coding for the kinse domain of the Elk receptor was amplified from TKB1 cells (Agilent Technologies, Santa Clara, CA) and cloned with a C-terminal HA-tag between NdeI and EcoRV restriction sites within the second open reading frame of the pCDF-Duet vector ...
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bioRxiv - Genomics 2019Quote: ... we only analyzed regions overlapping between RNA-seq (with more than 10x read coverage in annotated exons) and the WES enrichment kit (Agilent SureSelect 71Mb). Again ...
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bioRxiv - Immunology 2019Quote: A deleted version of pNL4-3 was constructed (18) that lacks the entire Gag and Protease region (Stratagene Quick-Change XL kit) replacing this region with a BstE II (New England Biolabs ...
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bioRxiv - Cell Biology 2021Quote: ... The N-terminal region was removed by a Quikchange reaction (Agilent), and the resulting shortened fragment was inserted into the pDEST-HisMBP destination vector via a Gateway reaction.
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bioRxiv - Cell Biology 2021Quote: ... PCR was performed across the targeted region using Herculase II (Agilent).
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bioRxiv - Immunology 2022Quote: Specific amino acid changes on envelope clones and CAP206-CH12 heavy chain CDRH3 were introduced using the QuikChange Lightning Site-Directed Mutagenesis Kit (catalog #210519, Agilent Technologies, CA, USA). Mutations were confirmed by sequence analysis.
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bioRxiv - Cancer Biology 2023Quote: ... The oxygen consumption rate (OCR) assay was performed using the XF long-chain fatty acid oxidation (LCFA) stress test kit (Agilent, cat# 103672-100) and Seahorse XFe24 Analyzer was used to investigate the long-chain fatty acid OCR according to the manufacturer’s protocol.
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bioRxiv - Biochemistry 2019Quote: ... a polymerase chain reaction using PfuUltra II Fusion DNA polymerase (Agilent Technologies), the template pIL-SVnisBTC and the appropriate pair of oligonucleotides (Supplementary File 4 ...