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Citations for Agilent :
201 - 250 of 2244 citations for Eukaryotic Peptide Chain Release Factor GTP Binding Subunit ERF3A GSPT1 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Mutations of OVOL1 binding sites were performed using QuikChange Lightning site-directed mutagenesis kits (Agilent).
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bioRxiv - Biochemistry 2020Quote: The DNA template was amplified by the polymerase chain reaction (PCR) using Pfu high-fidelity DNA polymerase (Agilent Technologies). Each 50 μL reaction consisted of gDNA (∼27 ng ...
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bioRxiv - Microbiology 2021Quote: ... Cassettes and the template plasmid pM07704 were amplified by polymerase chain reaction (PCR) with Herculase II DNA polymerase (Stratagene). Marker-exchange plasmids were generated by ligation of the PCR products in α-select cells (Bioline ...
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bioRxiv - Neuroscience 2020Quote: ... nucleotides: 907-1550) was amplified by the use of polymerase chain reaction (PCR) and cloned into pBluescript KS+ (Stratagene) via ClaI-EcoRI sites ...
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bioRxiv - Physiology 2023Quote: ... Hepatic portal vein bile acids and short chain fatty acids were acquired using an Agilent 1290 series HPLC (Agilent,) with an Acquity C18 BEH (2.1mm x 50mm ...
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bioRxiv - Microbiology 2023Quote: ... Real-time Polymerase Chain Reaction (qPCR) was conducted in a Stragene Mx3005P real-time PCR system (Agilent Technologies®) to analyze the relative expression of the cytokine genes Interleukin 2 (IL-2) ...
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bioRxiv - Biochemistry 2020Quote: ... Selected mRNAs were purified and reverse-transcripted into single-chain DNA with the primer 5’-CTTCAGTTGCCGCTTTCTTTCTTG-3’ using a reverse transcriptase (Cat 200436, Agilent). The resulting cDNA library was purified using a DNA purification kit (Cat A740609.25 ...
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bioRxiv - Biophysics 2022Quote: All of mutations in this study were made by using overlap-extension polymerase chain reaction (PCR) with Pfu polymerase (Stratagene) on the template of the mbr5 splice variant of mslo1 (Uniprot ID ...
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bioRxiv - Immunology 2020Quote: ... and bound mAbs were detected with rabbit anti-human IgG gamma chain-specific/HRP (Agilent: cat. P0214, 1:15,000 dilution).
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bioRxiv - Biochemistry 2021Quote: ... AccuScript high-fidelity first-strand cDNA synthesis kit for reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) was procured from Agilent.
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bioRxiv - Cell Biology 2023Quote: ... was purchased from Jackson ImmunoResearch Laboratories and was used for Western blotting. Affinity-purified rabbit polyclonal anti-human lambda-light chain (cat. A019302-2) was purchased from Dako and was used for Western blotting ...
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bioRxiv - Immunology 2023Quote: ... The variable heavy chain sequence of CH42 was subcloned into a plasmid coded with an IgG1 heavy chain backbone containing the N297A 29 mutation via QuikChange Site Directed Mutagenesis kit (Agilent). Antibodies were expressed through co-transfection of heavy and light chain plasmids in Expi293 HEK cells (Thermo Fisher ...
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Development of a genetically encoded sensor for probing endogenous nociceptin opioid peptide releasebioRxiv - Neuroscience 2024Quote: ... site directed mutagenesis and Circular Polymerase Extension Cloning was performed by polymerase chain reaction with custom designed primers using a Pfu-Ultra II fusion High Fidelity DNA Polymerase (Agilent). Sanger sequencing (Microsynth ...
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bioRxiv - Pathology 2024Quote: Twenty to fifty grams of frozen caecal contents were used to analyzed the short-chain fatty acids (SCFAs) composition using gas chromatography (Agilent 7890B gas chromatograph ...
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bioRxiv - Microbiology 2024Quote: Sequences coding for Fab fragments were obtained by inserting stop codons on genes corresponding to heavy chains of the mAbs by PCR using site directed mutagenesis (Quickchange II, Agilent) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... and bioenergetic profiles were measured with Real-Time ATP Rate Assay and Long Chain Fatty Acid (LCFA) Oxidation Stress Assay (Agilent). Concentrations of the used inhibitors were ...
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bioRxiv - Microbiology 2021Quote: ... Site-directed mutations in the putative Nla28 binding site were generated using the Quickchange system (Agilent) as previously described (5 ...
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bioRxiv - Biochemistry 2021Quote: ... Peptides were purified using a 1260 Infinity II preparative HPLC system (Agilent Technologies ...
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bioRxiv - Bioengineering 2020Quote: Peptides were purified by mass-directed semi-preparative reversed phase HPLC (Agilent). Solvent A was water with 0.1% TFA and solvent B was acetonitrile with 0.1% TFA ...
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bioRxiv - Genomics 2021Quote: ... Phosphorylated peptides were enriched on an AssayMAP Bravo automation platform (Agilent Technologies) using 5 μL Fe(III)-NTA cartridges (Agilent ...
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Biochemical alterations precede neurobehavioral deficits in a novel mouse model of Friedreich ataxiabioRxiv - Biochemistry 2021Quote: ... All peptide samples were analyzed on a triple quadrupole spectrometer (Agilent 6420) equipped with an electrospray ion source ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting peptides were desalted using OMIX C18 pipette tips (Agilent Technologies) or 1 mL C18 Sep Pak cartridges (Waters ...
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bioRxiv - Cell Biology 2021Quote: ... Peptides were separated by an Agilent 1200 series nanoflow system (Agilent Technologies) and analyzed using an LTQ Orbitrap classic mass spectrometer (Thermo Electron ...
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bioRxiv - Evolutionary Biology 2021Quote: ... using a telomere PNA (peptide nucleic acid) FISH Kit/FITC (DAKO, Denmark) according to the manufacturer’s protocol ...
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Structure of the Human Signal Peptidase Complex Reveals the Determinants for Signal Peptide CleavagebioRxiv - Biochemistry 2020Quote: Cross-linked peptides were enriched with Fe(III)-NTA cartridges (Agilent Technologies) using the AssayMAP Bravo Platform (Agilent Technologies ...
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bioRxiv - Physiology 2023Quote: ... Separation of peptides was conducted using a C18 column (Agilent, #959961-902) with reverse phase chromatography ...
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bioRxiv - Microbiology 2023Quote: ... Peptides containing supernatants were transferred in mass spectrometry vials (#9301-0978, Agilent).
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bioRxiv - Microbiology 2023Quote: ... tryptic peptides were bound to C18 resin (Bond Elut C18 cartridge, Agilent) in 0.1% TFA ...
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bioRxiv - Biophysics 2022Quote: ... Dissolved peptides were injected into an Agilent 1200 series HPLC system (Agilent Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... Peptide digests were injected onto a 1260 Infinity nHPLC stack (Agilent Technologies) and separated using a 75-micron I.D ...
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bioRxiv - Cancer Biology 2023Quote: ... The phosphorylated peptides enrichment was performed using Fe(III)-NTAcartridges (Agilent Technologies) using the AssayMAP Bravo Platform (Agilent Technologies)43 ...
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bioRxiv - Cancer Biology 2023Quote: ... The tryptic peptides were enriched and desalted using OMIX C18 tips (Agilent) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... The peptides were desalted by C18 using the Bravo AssayMAP platform (Agilent) and then deglycosylated and dephosphorylated overnight at 37 °C with the protein deglycosylation mix II (New England Biolabs ...
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bioRxiv - Biochemistry 2024Quote: ... All peptide samples were analyzed on a triple quadrupole spectrometer (Agilent 6420) equipped with an electrospray ion source ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting peptide mixtures were extracted and desalted using C18 tips (Agilent) prior to MS analyses.
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bioRxiv - Cancer Biology 2024Quote: ... peptide samples were first desalted and concentrated onto a µC18 Omix (Agilent) before analysis.
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bioRxiv - Cell Biology 2024Quote: Peptides were prepared as previously described using a BRAVO pipetting robot (Agilent) as per reference43 ...
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bioRxiv - Bioengineering 2021Quote: ... Mutations were cloned into the expression vector by polymerase chain reaction (PCR) using a PFUultra II Hotstart PCR Master Mix (Agilent Technologies). The obtained PCR products were subsequently treated with DPNI (New England Biolabs ...
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bioRxiv - Physiology 2021Quote: Two truncated recombinant fibrinogen α-chain variants (α220 and α390) were produced using QuikChange II Site-Directed Mutagenesis Kits from Agilent Technologies (Stockport ...
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bioRxiv - Cell Biology 2022Quote: ... full-length cDNAs of the zebrafish banp gene were amplified with Polymerase Chain Reaction (PCR) and sub-cloned into pBluescript II SK(+) vectors (Stratagene/Agilent Technologies). cDNA fragments were amplified from mRNA with PCR using the primers for cenpt ...
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bioRxiv - Immunology 2020Quote: ... The RM19R Fab heavy chain plasmid was made introducing two stop codons following residue D234 in the RM19R IgG1 heavy chain vector using the QuikChange® Lightning Site-Directed Mutagenesis kit (Agilent). The RM19R Fab was expressed in HEK293F cells (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... Fab expression vectors were made by introducing two stop codons following residue D234 (Kabat numbering 57) in the IgG heavy chain vectors using the QuikChange® Lightning Site-Directed Mutagenesis kit (Agilent). Sequences were verified by Sanger sequencing (Genewiz).
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bioRxiv - Microbiology 2021Quote: Short-chain fatty acids were analyzed by gas chromatography with a flame ionization detector (GC-FID 7890 A Agilent Technologies Inc.) with a fused silica capillary column (Nukon SUPELCO ...
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bioRxiv - Systems Biology 2023Quote: ... bile acids and short chain fatty acids analyses were performed by LC-MS/MS with a 1260 UHPLC (Ultra-High Performance Liquid Chromatography) (Agilent Technologies) coupled to a QQQ 6410 (Agilent Technologies ...
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bioRxiv - Genetics 2024Quote: A UTR library of DNA templates was assembled by overlapping extension polymerase chain reaction (PCR) using Herculase II Fusion Enzyme (Agilent Technologies). Initially ...
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bioRxiv - Cancer Biology 2020Quote: ... After blocking nonspecific-binding sites on sections with Protein Block Serum-Free reagent (Dako North America Inc.) for 30 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... Cholesterol and its influence on Gb3 binding were analyzed by targeted LC-QqQ-MS (6460, Agilent Technologies) operated in SRM/MRM mode ...
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bioRxiv - Cancer Biology 2022Quote: ... After blocking nonspecific-binding sites on sections with Protein Block Serum-Free reagent (Dako North America Inc.) for 30 minutes ...
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bioRxiv - Developmental Biology 2020Quote: ... Mutagenesis of the putative Sp1 binding sites was performed using the QuikChange Site-Directed Mutagenesis Kit (Stratagene). All the reporter constructs were inserted and analyzed at the same landing attP site ...
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bioRxiv - Biochemistry 2023Quote: ... The maltose-binding protein (MAL) gene from Escherichia coli (gene identifier 7129408) in a pBlueScript KS (Stratagene) was amplified by PCR using T7 minimal promoter primers under the following conditions ...