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Citations for Agilent :
101 - 150 of 2244 citations for Eukaryotic Peptide Chain Release Factor GTP Binding Subunit ERF3A GSPT1 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... thyroid transcription factor (TTF-1) (mouse, M3575, 1:100; Dako), and p40 (rabbit ...
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bioRxiv - Cancer Biology 2024Quote: ... and angiogenesis markers (Factor-VIII, DAKO A0082; Ki67, DAKO 7240). Vessels from adjacent normal tissue were used as controls ...
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bioRxiv - Cancer Biology 2024Quote: ... and angiogenesis markers (Factor-VIII, DAKO A0082; Ki67, DAKO 7240). Vessels from adjacent normal tissue were used as controls ...
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bioRxiv - Genomics 2020Quote: ... HNRNPM binding sites were mutated using QuikChange XLII Mutagenesis Kit (Stratagene). For flow cytometry assays ...
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bioRxiv - Cancer Biology 2021Quote: ... binding was detected with the diaminobenzidine-peroxidase visualization system (EnVision+, Dako). Mayer’s hematoxylin was used for counterstaining ...
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bioRxiv - Bioengineering 2022Quote: ... binding buffers and processed on Agilent 2100 Bioanalyzer (Agilent CA, USA) to determine the yield and size distribution of each library.
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bioRxiv - Cell Biology 2024Quote: ... Non-specific binding was blocked with Protein block serum-free (Dako) and cells were stained with the primary antibody ...
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bioRxiv - Neuroscience 2020Quote: Site-directed insertions were made in GluN1 (GluN1-a) (NCBI Protein database accession no. P35439) or GluN2A (Q00959) subunits with the QuikChange site-directed mutagenesis kit (Agilent) with XL1-Blue super-competent cells ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Gα subunit plasmids were purchased from cdna.org (Bloomsburg University, Bloomsburg, PA. 4A insertion mutations were introduced to Gα subunits using the QuikChange Mutagenesis Kit (Agilent Technologies) and oligonucleotides (Integrated DNA Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... Point amino acid substitutions in genes encoding GluN subunits were performed using the QuikChange site-directed mutagenesis kit (Agilent Technologies) and verified by DNA sequencing.
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bioRxiv - Microbiology 2021Quote: ... Heavy chain Y58F or F58Y mutants were constructed using the QuikChange XL Mutagenesis kit (Stratagene) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... The polymerase chain reaction (PCR) was conducted using Herculase II Fusion DNA Polymerase (Agilent, USA). The sequences of both f- and h-Yotiao were acquired from the National Center for Biotechnology Information (NCBI) ...
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bioRxiv - Cell Biology 2021Quote: ... Peptides were purified on Omix C18 tips (Agilent), dried and re-dissolved in 20 µl loading solvent A (0.1% trifluoroacetic acid in water/acetonitrile (ACN ...
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bioRxiv - Bioengineering 2022Quote: ... Peptides were purified with preparative HPLC (Agilent 1260) with Agilent ZORBAX 300 SB-C18 (9.4 x 250 mm ...
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bioRxiv - Cell Biology 2021Quote: ... Peptides were purified on Omix C18 tips (Agilent), dried and re-dissolved in 20 µl loading solvent A (0.1% trifluoroacetic acid in water/acetonitrile (ACN ...
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bioRxiv - Molecular Biology 2021Quote: ... peptides were purified on OMIX C18 Tips (Agilent) which were first washed with pre-wash buffer (0.1% TFA in water/acetonitrile (20:80 ...
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bioRxiv - Bioengineering 2021Quote: ... Peptides were purified with preparative HPLC (Agilent 1260) and identified by Shimadzu LCMS-2020 ...
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bioRxiv - Biochemistry 2023Quote: ... peptides were desalted using C18 OMIX tips (Agilent) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: Digested peptides were desalted using C18 cartridges (Agilent Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... Peptides were desalted using an AssayMAP Bravo (Agilent) liquid handler and AssayMAP 5 µL C18 cartridges (Agilent 5190-6532) ...
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bioRxiv - Microbiology 2024Quote: ... such as the uncut PT oligos and the four 3-mer release oligos (CCA, CCC, CCG, and CCT) using an HPLC system (Agilent 1290) as outlined below ...
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bioRxiv - Bioengineering 2020Quote: ... anti-von Willebrand factor (rabbit polyclonal, Dako # A0082, diluted 1:100), anti-Pax7 (mouse monoclonal ...
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bioRxiv - Cell Biology 2022Quote: ... Anti human Von Willebrand Factor (vwf) was purchased from Dako(Denmark).15ml and 50ml centrifuge tube were purchased from Nest Biotechnology Co.,Ltd (China).Glass bottom cell culture dish was purchased from Guangzhou saiguo biotech Co.,Ltd (China) ...
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bioRxiv - Biophysics 2020Quote: ... a peptide trap column (OPTI-TRAP for peptides, Optimize Technologies) and a reversed-phase analytical column (PLRP-S for Biomolecules, Agilent Technologies). Pepsin digestion was performed online at a flow rate of 75 µL/min (0.4 % formic acid in water ...
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bioRxiv - Cell Biology 2023Quote: The NR2A(M705V) or NR2A(A727T) mutation was introduced to the NR2A subunit using QuikChange II site-directed mutagenesis Kit (Agilent Genomics). DNA sequencing was used to confirm the cDNA sequences ...
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bioRxiv - Microbiology 2020Quote: ... p-nitrophenyl-β-D-xylopyranoside and p-nitrophenyl-α-D-xylopyranoside were carried out by estimating the release of 4-nitrophenol (pNP) at 405 nm using a UV-Vis spectrophotometer (Agilent, G 68608) as described elsewhere (11).
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Mechanical forces impair antigen discrimination by reducing differences in T cell receptor off-ratesbioRxiv - Immunology 2022Quote: ... TCR α and β chains were expressed in BL21 (DE3)-RIPL Escherichia coli cells (Agilent Technologies) following induction with 0.15 mM IPTG ...
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bioRxiv - Cancer Biology 2022Quote: We used the Seahorse XF Long Chain Fatty Acid Oxidation Stress Test kit (Agilent, 102720-100). Cells were incubated in assay media supplemented with 150 µM BSA or oleic acid conjugated to BSA as fatty acid substrate in the following formulation ...
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Sex and species associated differences in Complement-mediated immunity in Humans and Rhesus macaquesbioRxiv - Immunology 2023Quote: ... Mutations in the wild type heavy chain plasmid were performed using site directed mutagenesis (Agilent, 200524) following the manufacturer’s protocol to generate EG ...
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bioRxiv - Microbiology 2020Quote: ... of sample was loaded via the autosampler onto a C18 peptide column (AdvanceBio Peptide 2.7 um, 2.1 x 150 mm, Agilent part number 653750-902) enclosed in a thermostatted column oven set to 50 °C ...
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bioRxiv - Immunology 2021Quote: ... Released peptides were desalted using AssayMap C18 cartridges (Agilent). Samples were stored at −20 °C prior to DIA-SWATH-MS analysis
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bioRxiv - Cell Biology 2021Quote: ... Peptide clean-up was done using OMIX C18 (Agilent) according to manufacturer protocol ...
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bioRxiv - Neuroscience 2022Quote: ... The peptides were cleaned with BondElut Omix Tips (Agilent) and concentrated in a vacuum concentrator prior to analysis by mass spectrometry.
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bioRxiv - Neuroscience 2023Quote: ... Peptides were purified on SampliQ SPE C18 cartridges (Agilent), dried and re-dissolved in 20 µl loading solvent A (0.1% trifluoroacetic acid in water/acetonitrile (ACN ...
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bioRxiv - Cell Biology 2023Quote: ... peptides were purified on SampliQ SPE C18 cartridges (Agilent). Columns were first washed with 1 ml 100% acetonitrile (ACN ...
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bioRxiv - Physiology 2024Quote: cDNA encoding the mouse γ subunit was mutated to replace 140RKRR143 with 140QQQQ143 using the QuckChange II XL mutagenesis kit (Agilent Technologies). cDNAs encoding ENaC’s mouse α ...
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bioRxiv - Cell Biology 2024Quote: ... and F272fs287X (c.813del) in the GABAAR α1 subunit were constructed using a QuikChange II site-directed mutagenesis Kit (Agilent Genomics, #200523), and the cDNA sequences were confirmed by DNA sequencing.
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bioRxiv - Neuroscience 2022Quote: ... and manufacturer’s instructions followed for protein binding and elution (Agilent, Santa Clara, CA). The low abundance protein fraction was further fractionated by 1D SDS PAGE and analyzed using LC-MS/MS ...
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bioRxiv - Microbiology 2023Quote: ... followed by saturation of nonspecific binding sites with normal goat serum (X0907; Agilent) applied for 25 min at room temperature ...
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bioRxiv - Immunology 2022Quote: For the analysis of Fcγ-receptor binding PE-Streptavidin (Agilent Technologies, CA, USA) was coupled to recombinant and biotinylated human FcγR2A ...
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Short-range interactions between fibrocytes and CD8+ T cells in COPD bronchial inflammatory responsebioRxiv - Pathology 2023Quote: ... Nonspecific binding was minimized by incubating the sections with 4% Goat Serum (Agilent) for 30 min ...
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bioRxiv - Immunology 2022Quote: ... Unspecific binding sites were blocked with Normal Goat Serum (1:5 dilution, DAKO) in antibody diluent (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: The efficiency of Dex encapsulation in Lipo and its subsequent release was evaluated with high-performance liquid chromatography (HPLC) using the 1260 Infinity II LC System (Agilent Technologies, California, US). A Luna® 5 μm C18(2 ...
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bioRxiv - Bioengineering 2024Quote: ... The amount of BACSM in the removed release medium was then assessed by UPLC using a Poroshell C18 (Agilent Technologies, Santa Clara, CA) UPLC column and adjusting for the volume remaining in the well after each sampling (Supplementary Information Table S1) ...
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bioRxiv - Immunology 2022Quote: ... Vectors encoding the Fab light chains FenAB609 and FenAB709 were generated by QuikChange site-directed mutagenesis (Agilent QuikChange Lightning site-directed mutagenesis kit ...
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bioRxiv - Biochemistry 2021Quote: ... All mutant genes were constructed by polymerase chain reactions using a QuickChange Site-Directed Mutagenesis kit (Stratagene) and primers (Table S1) ...
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bioRxiv - Biochemistry 2024Quote: ... and the terminal tailpiece of the IgM μ chain (pcDNA3.1(+)-Cμ2Cμ3Cμ4Tp) was obtained using site-directed mutagenesis (QuickChange II XL kit, Agilent) to delete the sequence of the variable and Cμ1 domains from the pcDNA3.1(+)-IgM012 H chain vector ...
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bioRxiv - Immunology 2024Quote: ... The reaction was stopped with trifluoroacetic acid, and peptides were analyzed by RP-HPLC (Shimatzu, Kyoto, Japan) on an AdvanceBio Peptide Plus C18 column (Agilent Technologies, Santa Clara, CA). The samples (100 µL ...
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bioRxiv - Cell Biology 2022Quote: ... or C166A/C180A in the GABAA receptor α1 subunit) were constructed using a QuikChange II site-directed mutagenesis Kit (Agilent Genomics, catalog #: 200523). A FLAG tag was inserted between Leu31 and Gln32 of the α1 subunit and between Asn28 and Asp29 of the β2 subunit of GABAA receptors by using QuikChange II site- directed mutagenesis ...
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bioRxiv - Genetics 2021Quote: ... Resulting peptides were desalted by OMIX C18 desalting tips (Agilent) following protocol by provider and dried on a speed-vac ...