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Citations for Agilent :
401 - 450 of 2244 citations for Eukaryotic Peptide Chain Release Factor GTP Binding Subunit ERF3A GSPT1 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Antibodies were diluted in antibody diluent (Dako, S0809) containing 0.3% Triton X-100 and incubated overnight at 4 °C in a humidified chamber ...
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bioRxiv - Developmental Biology 2023Quote: ... Primary antibodies were diluted in Antibody Diluent (Dako) and incubated overnight at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies were diluted in Antibody Diluent (Dako) and a droplet of 25 µl or 50 µl applied on the sample for an 8-well or a coverslip upside down ...
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bioRxiv - Systems Biology 2020Quote: ... lyophilized samples were resuspended in Buffer A (5 mM NH4HCO2/ 2% ACN) and 5 mg peptide material (5 mg/ml) was loaded onto a reversed phase column (ZORBAX 300Extend-C18, Agilent). Peptides were separate at a flow rate of 2 ml/min and a constant column temperature of 40 °C using a binary buffer system ...
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bioRxiv - Cell Biology 2021Quote: Ap-proximately 1000 ng of peptides from each biological replicate before SCX fractionation were first injected onto an Agilent 1290 Infinity UHPLC system (Agilent) on a 50-cm analytical column packed with C18 beads (Dr Maisch Reprosil C18 ...
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bioRxiv - Genomics 2022Quote: ... The total peptide concentration was measured by UV absorbance at 210 nm using an LC system with UV detector (Agilent), with roughly 10 mg tissue yielding 500-750 μg peptide mixture.
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bioRxiv - Bioengineering 2021Quote: Peptides used for pMHC refolding were synthesized in housed using the Liberty Blue Peptide Synthesizer (CEM) and validated using LC-MS (Agilent). To generate pMHC molecules for bioconjugation ...
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bioRxiv - Plant Biology 2020Quote: ... The dried peptides were dissolved in 1% TFA solution to perform zip-tip cleanup using C-18 Omix tips (Agilent). Digests were dried in a speedvac as elution-2 (E2 ...
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bioRxiv - Molecular Biology 2020Quote: ... samples were passed through an immobilized pepsin column (2mm 2cm) at 200 μl min−1 and the digested peptides were captured on a 2mm 1cm C8 trap column (Agilent) and desalted ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The peptides were then purified and concentrated using OMIX Tips C18 reversed-phase resin (Agilent Technologies, Santa Clara, CA, USA). The peptides were dehydrated in a vacuum centrifuge and analysed by NanoLC-MS/MS ...
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bioRxiv - Immunology 2023Quote: Fluorophore-conjugated (or biotinylated) peptides were purified via reversed phase chromatography on a C18 column (Pursuit XRs C18 column, Agilent) to separate peptide-dye conjugates from unconjugated peptides or dyes ...
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bioRxiv - Plant Biology 2023Quote: ... 10 μg of TMT-labeled peptides were loaded onto 10 cm capillary columns packed with 5 μM Zorbax SB-C18 (Agilent), which was connected using a zero dead volume 1 μm filter (Upchurch ...
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bioRxiv - Systems Biology 2023Quote: ... and digested using a modified in-solution sample preparation protocol.94 One hundred micrograms of desalted peptides were fractioned on an microflow HPLC (1260, Agilent) using a XBridge peptide BEH C18 column (130 Å ...
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bioRxiv - Systems Biology 2023Quote: ... equivalent amounts of each digested sample were pooled before proceeding to a peptide fractionation using a manual workflow based on Stage-Tips or an automatic workflow with the AssayMAP Bravo (Agilent).
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bioRxiv - Molecular Biology 2022Quote: ... at 200 µl min-1 and the digested peptides were captured on a 2 mm x 1 cm C8 trap column (Agilent) and desalted ...
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bioRxiv - Molecular Biology 2024Quote: ... quenched samples were passed through a co-immobilized pepsin/fungal XIII protease column (1mm × 2cm) at 50 µl min−1 and the digested peptides were captured on a 1mm × 1cm C8 trap column (Agilent) and desalted ...
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bioRxiv - Cancer Biology 2024Quote: ... enrichment of phosphorylated peptides was performed on the Bravo automated liquid handling platform using AssayMAP Fe(III)-NTA cartridges (Agilent). In brief ...
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bioRxiv - Cell Biology 2024Quote: ... pcDNA3.1zeo+/ nFlag-cmScarlet-hRobo1 was generated by inserting the Flag tag sequence at the N-terminus after the signal peptide sequence of Robo1 with QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies ...
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bioRxiv - Microbiology 2023Quote: ... Solid phase extraction of peptides was done using equilibrated C18 pipette tips (Bond Elut OMIX 100 µl pipette tips, Agilent). The samples are aspirated for 10 cycles for maximum binding efficiency and then eluted in LC-MS/MS vials with 100 µl of 0.1% TFA in water:acetonitrile (at a ratio of 30:70 (v/v)) ...
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bioRxiv - Plant Biology 2023Quote: ... and portions of samples corresponding to 5 µg of peptide were analyzed by nanoflow reverse-phase liquid chromatography-mass spectrometry using a 15 cm C18 Zorbax column (Agilent), a Dionex Ultimate 3000 RSLC nano-UPLC system ...
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bioRxiv - Bioengineering 2023Quote: ... Peptides were loaded to a nanoscale HPLC column composed of 10 cm of Polaris C18 5 μm packing material (Agilent), followed by 4 cm of Partisphere 5 SCX (Whatman) ...
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bioRxiv - Biochemistry 2022Quote: Purity of the wild-type or mutant FAM237A peptides was analyzed by HPLC using an analytical C18 reverse-phase column (Zorbax 300SB-C18, 4.6 × 250 mm, Agilent Technologies). The FAM237A peptides were eluted from the C18 reverse-phase column by an alkaline acetonitrile gradient composed of alkaline solvent A and B [15] ...
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bioRxiv - Biochemistry 2022Quote: ... Peptide fractions were eluted from a semi-preparative C18 reverse-phase column (Zorbax 300SB-C18, 9.4 × 250 mm; Agilent Technologies) by an acetonitrile gradient ...
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bioRxiv - Cell Biology 2023Quote: ... the HA epitope sequence was inserted immediately 3’ to the signal peptide sequence by site directed mutagenesis using the QuickchangeXL site directed Mutagenesis kit (Agilent) to obtain the following intermediate vector ...
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bioRxiv - Physiology 2024Quote: The mixture of labeled peptides was subjected to reverse-phase LC fractionation using an Agilent 1200 Series HPLC (Agilent, USA) to obtain 24 fractions then fractions were combined (1 and 13 ...
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bioRxiv - Plant Biology 2024Quote: ... Portions of samples corresponding to 5 µg of peptide were analyzed by nanoflow reverse-phase liquid chromatography-mass spectrometry using a 15 cm C18 Zorbax column (Agilent), a Dionex Ultimate 3000 RSLC nano-UPLC system and the Orbitrap Fusion Lumos Tribrid Mass Spectrometer (Thermo) ...
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bioRxiv - Molecular Biology 2024Quote: ... For phospho-peptide enrichment 200 µg of peptides in 100 µL were subjected to enrichment using the default phospho-peptide enrichment protocol from the AssayMAP Bravo Platform (Agilent). For the enrichment 50 ml of 0.1% formic acid (FA ...
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bioRxiv - Immunology 2024Quote: 0.4 ug of biotinylated SL9 (SLYNTAVTL) or IV9 (ILKEPVHGV) peptides (NIH Tetramer Core Facility) were conjugated to PE-streptavidin (Agilent), APC-streptavidin (Agilent ...
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bioRxiv - Systems Biology 2024Quote: ... Offline fractionation of TMT-labeled peptides was performed using a high-pH reversed-phase gradient with a ZORBAXTM C18 column (Agilent, 250 mm length/4.6 mM inner diameter/3.5 µm particle size ...
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bioRxiv - Biochemistry 2024Quote: ... The mature CXCL17 peptide was eluted from a C18 reverse-phase column (Zorbax 300SB-C8, 9.4 × 250 mm, Agilent Technologies) by an acidic acetonitrile gradient and manually collected ...
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bioRxiv - Cell Biology 2024Quote: Global phosphopeptide enrichment started from 200 µg peptides using IMAC cartridges (AssayMAP Fe(III)-NTA) on a Bravo Assaymap liquid handler (Agilent). Phosphopeptides were dried in a vacuum centrifuge and dissolved in 20 µl 0.5% TFA/4% ACN prior to injection ...
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bioRxiv - Cancer Biology 2021Quote: Antibodies were diluted in antibody diluent (Dako, S302281-2). Nuclei were visualized by DAPI (Sigma ...
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bioRxiv - Immunology 2022Quote: ... Antibodies were diluted in Background Reducing Antibody Diluent (Agilent). The tissue was subsequently incubated with an anti-rabbit HRP polymer (Leica ...
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bioRxiv - Bioengineering 2021Quote: ... All antibodies were diluted in Dako Antibody Diluent (Dako Antibody Diluent S0809 ...
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bioRxiv - Immunology 2021Quote: ... Antibodies were diluted in Background Reducing Antibody Diluent (Agilent). The tissue was subsequently incubated with an anti-rabbit HRP polymer (Leica ...
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bioRxiv - Microbiology 2022Quote: ... primary antibodies and appropriate HRP-conjugated secondary antibodies (DAKO). A chemiluminescence substrate (West Dura ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibodies were diluted in antibody diluent (Agilent; #S3022) and incubated with sections for 16 hours at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies diluted in antibody diluent (S080983-2, Dako) were applied on sections for overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... antibodies were diluted in Background Reducing Antibody Diluent (Agilent) and subsequently incubated with an anti-rabbit HRP polymer (Leica ...
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bioRxiv - Cell Biology 2024Quote: ... Primary Antibodies: anti-human CD41 antibody (Agilent, F708801-2) and alpha tubulin antibody (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibody staining in Background-Reducing Antibody Diluent (Agilent) with appropriate serum overnight at 4°C in a humid chamber ...
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bioRxiv - Molecular Biology 2021Quote: Cross-linked peptides were enriched with Fe(III)-NTA 5 µL in an automated fashion using the AssayMAP Bravo Platform (Agilent Technologies). Fe(III)-NTA cartridges were primed with 250 µL of 0.1% TFA in ACN and equilibrated with 250 µL of loading buffer (80% ACN/0.1% TFA) ...
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bioRxiv - Immunology 2022Quote: ... samples were acidified with formic acid (FA) and subsequently peptides were desalted using AssayMap C18 cartridges mounted on an AssayMap Bravo Platform (Agilent Technologies). Two biological replicates were pooled together to generate 100 micrograms of total peptide for TMT labeling and subsequent fractionation and phosphopeptide enrichment ...
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bioRxiv - Systems Biology 2020Quote: ... 1 μl of each sample containing 2 μg of total native peptides and 100 fmol of each standard peptide was loaded on a precolumn Zorbax 300SB-C18 (5 μm, 5 × 0.3 mm) (Agilent Technologies, USA) and washed with 5 % acetonitrile for 5 min at a flow rate of 10 μl/minute before analytical LC separation ...
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bioRxiv - Systems Biology 2020Quote: ... 3 μl of each sample containing approximately 10 μg of total native peptides and 500 fmol of each stable isotope-labeled standard (SIS) peptide were loaded on an analytical column, Zorbax 300SB-C18 (5 μm, 150 × 0.3 mm) (Agilent Technologies, USA) and washed with 5% acetonitrile for 5 min at a flow rate of 20 μl/min ...
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bioRxiv - Physiology 2021Quote: ... This was used to extract mass isotope abundances (M0-M3) of each peptide from MS-only acquisition files with Mass Hunter Qualitative Analysis software (Agilent Technologies). Mass isotopomer distribution analysis (MIDA ...
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bioRxiv - Cell Biology 2020Quote: ... This was used to extract mass isotope abundances (M0-M3) of each peptide from MS-only acquisition files with Mass Hunter Qualitative Analysis software (Agilent Technologies). Mass isotopomer distribution analysis (MIDA ...
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bioRxiv - Biophysics 2020Quote: ... Peptides were trapped in a trap column (OPTI-TRAP for peptides, Optimize Technologies) and separated on a reversed-phase analytical column (PLRP-S for Biomolecules, Agilent Technologies) using a 27 min gradient of 8-40 % organic solvent (0.4 % formic acid in acetonitrile ...
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bioRxiv - Biochemistry 2021Quote: ... The beads were then pulled down and the solutions containing the digested peptides were desalted using AssayMap C18 cartridges mounted on an AssayMap Bravo liquid handling system (Agilent Technologies) and subsequently dried down in a SpeedVac concentrator.
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bioRxiv - Physiology 2020Quote: ... This was used to extract mass isotope abundances (M0-M3) of each peptide from MS-only acquisition files with Mass Hunter Qualitative Analysis software (Agilent Technologies). Mass isotopomer distribution analysis (MIDA ...