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Citations for Agilent :
1951 - 2000 of 2480 citations for Ataxin 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... live cells stained with Hoechst 33342 (1:2,000 dilution) (ThermoScientific, Cat#62249) were enumerated using Cytation 5 plate reader (BioTek Instruments - Agilent). Values obtained from the Mito-Stress test were normalized per 1,000 cells.
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated overnight at 4 °C with the primary antibody: GFAP (Agilent/Dako, z033420-2, dilution 1:750) or MOG (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated overnight at 4 °C with the primary antibody: GFAP (Agilent/Dako, z033420-2, dilution 1:750) or MOG (Sigma-Aldrich ...
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bioRxiv - Physiology 2024Quote: ... Rehydrated 5-μm sections were stained with primary antibodies against insulin (dilution 1:1000; Dako, Santa Clara, CA, USA), somatostatin (dilution 1:300 ...
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bioRxiv - Immunology 2024Quote: ... coated XFe96 plate with fresh XF media (Seahorse XF RPMI medium containing 10 mM glucose, 2 mM L-glutamine, and 1 mM sodium pyruvate, PH 7.4; all reagents from Agilent). OCR was measured in the presence of Oligomycin (1.5 μM ...
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bioRxiv - Cell Biology 2024Quote: ... GC-FID was performed using an Agilent 7890 gas chromatograph equipped with a DB-WAX column (15 m x 0.53 mm, 1 µm; Agilent) and flame ionization detection ...
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bioRxiv - Plant Biology 2020Quote: ... The digest was filtered (0.22 μm) and filtered samples (1-2 μg) were loaded onto a C18 high capacity nano LC chip (Agilent Technologies) using a 1200 series capillary and eluted directly into a 6550 series quadrupole time-of-flight mass spectrometer (Agilent Technologies ...
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bioRxiv - Plant Biology 2021Quote: ... 1 μL of sample was injected into the GC (Sigma-Aldrich 5973 Network mass selective detector attached to an Agilent 6890 gas chromatograph (Agilent Technologies ...
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bioRxiv - Plant Biology 2021Quote: ... Two µl of samples were introduced in the splitless injection mode in DB-5 (60 m × 0.25 mm, 1 µm film thickness, Agilent, CA) using helium as a carrier gas ...
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mTOR inhibition enhances delivery and activity of antisense oligonucleotides in uveal melanoma cellsbioRxiv - Cancer Biology 2021Quote: ... or 1 µM (OMM1) carbonyl cyanide 4-(trifluoromethoxy)-phenylhydrazone (FCCP) and 0.5 µM of a rotenone antimycin A mix (Agilent Technologies). Following the assay ...
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bioRxiv - Cancer Biology 2021Quote: ... The plate was incubated in a CO2-free 37°C incubator for 1 hour and a Mitochondrial Stress Test (Agilent) was performed according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... S34F or Q157R were treated with doxycycline (1 μg/mL) for 48 hours before UV-crosslinking (400 mJ/cm2 UV Stratalinker 2400, STRATAGENE). RNA-protein complexes were immunoprecipitated with 12 μg anti-FLAG M2 antibody (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... the specimens were detected by incubating with goat anti-rabbit or anti-rabbit/mouse IgG-HRP for 1 h and subsequently with DAB (Dako). The tissue sections were then counterstained with 0.1% (wt/vol ...
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bioRxiv - Cell Biology 2020Quote: ... ∼100 separate PCR reactions (library and representation dependent) were performed with 1 μg genomic DNA in each reaction using Herculase II Fusion DNA Polymerase (Agilent) or Phusion High-Fidelity DNA Polymerase (Thermo Fisher) ...
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bioRxiv - Microbiology 2021Quote: ... and the cDNA hybridised to an 8 Chamber Agilent slide at 65°C for 16 hours before washing the slide with Oligo aCGH Wash Buffer 1 (Agilent) for 5 minutes at room temperature and Oligo aCGH Wash Buffer 2 (Agilent ...
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bioRxiv - Molecular Biology 2021Quote: ... The TIMS elution voltage was linearly calibrated to obtain 1/K0 ratios using three ions from the ESI-L Tuning Mix (Agilent) (m/z 622 ...
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bioRxiv - Molecular Biology 2021Quote: ... The TIMS elution voltage was linearly calibrated to obtain 1/K0 ratios using three ions from the ESI-L Tuning Mix (Agilent) (m/z 622 ...
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bioRxiv - Developmental Biology 2020Quote: ... then 1 ml of the headspace gas was withdrew and injected into a gas chromatograph (Agilent GC 6890N, CA, USA) fitted with a flame ionization detector and an activated alumina column (Zhang et al. ...
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bioRxiv - Neuroscience 2021Quote: ... We used additional microglial markers including mouse anti-MHC-II/HLA-DP/DQ/DR (1:250, M077501-2, Agilent, UK), rabbit anti-PU.1 (1:200 ...
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bioRxiv - Molecular Biology 2021Quote: ... Size of chromatin fragments was verified on a 1% agarose gel and on a high sensitivity DNA chip in a 2100 bioanalyzer (Agilent) after decrosslinking overnight at 65°C with shaking in elution buffer (100 mM NaHCO3 ...
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bioRxiv - Molecular Biology 2021Quote: ... HRP-conjugated secondary antibodies were then incubated on slides for 1-hour and detected with the DAB+ chromogen kit (DAKO). After washing ...
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bioRxiv - Molecular Biology 2021Quote: Reverse transcription for PCR analysis was performed using 1 μg total RNA and 300 ng random hexamers using Affinity script multiple temperature reverse transcriptase (Agilent). For UTP11 ...
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bioRxiv - Immunology 2022Quote: ... for 1 hour RT and replacing the secondary antibody incubation with HRP-labeled polyclonal goat α-mouse antibody (Dako K4000) for 30 minutes RT ...
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bioRxiv - Microbiology 2020Quote: ... EnvP(b)1CS- with the furin cleavage site mutated from RKTR to SKTR was generated from the human EnvP(b)1 plasmid using QuikChange site-directed mutagenesis (Agilent). EnvP(b)1 iRBD sequences were synthesized and cloned into a modified pVRC8400 expression vector ...
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bioRxiv - Immunology 2019Quote: ... overnight at 4 °C followed by a 1 h incubation with either an HRP-conjugated goat anti-mouse IgG or goat anti-rabbit IgG (Dako). Proteins were detected using Luminata™ Forte Western HRP Substrate (Millipore ...
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bioRxiv - Immunology 2019Quote: ... Immunofluorescence staining was carried out using the following antibodies: a primary rabbit anti-GFAP (resting and reactive astrocytes, 1:500 dilution, Z0334, Dako), and a primary rabbit anti-Iba1 (resting and activated microglia/macrophages ...
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bioRxiv - Plant Biology 2019Quote: ... Samples were then diluted 1:11 in ultrapure water before analysis with ICP-OES (Vista-PRO CCD Simultaneous ICP-OES; Agilent). Calibration was carried out using standards of Zn ...
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bioRxiv - Cell Biology 2020Quote: ... The membranes were incubated in the given primary antibody in a dilution of 1:1000 and appropriate HRP-conjugated secondary antibody (Dako) in a dilution of 1:1500 ...
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bioRxiv - Microbiology 2019Quote: ... The resulting products were then run with a Fragment Analyzer capillary electrophoresis using the DNF905 1-500 base pair kit (Agilent). Quantification was performed using the following equation ...
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bioRxiv - Molecular Biology 2019Quote: ... and the beads were resuspended in 50 μL Luminex block buffer containing a 1:5,000 dilution of streptavidin-phycoerythrin (Prozyme #PJ31S). The plate was shaken at 1100 RPM for 30 sec and incubated for 30 min at 600 RPM ...
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bioRxiv - Microbiology 2019Quote: UV-Visible spectra were recorded in 1 cm optic path quartz cuvette under aerobic conditions on a Cary 100 UV-Vis spectrophotometer (Agilent) and under anaerobic conditions in a glove box on a XL-100 Uvikon spectrophotometer equipped with optical fibers.
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bioRxiv - Cancer Biology 2019Quote: ... single strand cDNA synthesis and PCR amplification were carried out in a 1-step reaction using the Brilliant II QRT-PCR Master Mix (Agilent) and TaqMan gene expression assays (Life Technologies) ...
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bioRxiv - Cell Biology 2019Quote: HEK293T cells were seeded in 96-well plates (1×104 cells/well) (Thermoscientific, 136101) and transfected with pNF-κB-Luc (Stratagene) and phRK-TK (Renilla ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the CMV enhancer was amplified with the primers P322 and P323 using the phrGFPII-1 plasmid (Agilent Technologies, Santa Clara) as template ...
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bioRxiv - Neuroscience 2020Quote: ... IdU) on alternate one-in-ten sections using different anti-BrdU antibodies from different vendors (for BrdU: 1/200, Dako, Glostrup ...
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bioRxiv - Cell Biology 2021Quote: The 3a ORF from SARS CoV-1 (Tor2 strain) was originally obtained from the Institute for Genomic Research and subcloned into pBluescript (Stratagene) prior to transferring to pCAGGS-MCS [17] after PCR amplification with KpnI and XhoI restriction sites ...
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bioRxiv - Cell Biology 2021Quote: ... RNA concentration was measured by Nanodrop and 1 to 3 μg of RNA was reverse transcribed with AffinityScript Multi-Temp RT (Agilent) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... and retrovirus expressing EGFP in alternating one-in ten sections using different anti-BrdU antibodies from different vendors (for BrdU: mouse primary at 1/100, Dako; CldU ...
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bioRxiv - Molecular Biology 2021Quote: ... by inserting synthesized coding sequence for full length hRpn13 (NM_007002.3) or for residues 1–279 between the BamHI and HindIII restriction sites of pCMV-3Tag-1a (Agilent Technologies, 240295). Unmodified pCMV-3Tag-1a was used as empty vector (EV ...
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bioRxiv - Biophysics 2021Quote: ... The samples were heated up with a ramp rate of 1 °C/min over a temperature range of 15-95 °C using the qPCR System MX 3005 P (Stratagene). Measurements were performed in triplicate ...
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bioRxiv - Biochemistry 2021Quote: Rat Kir6.1 and N-terminal FLAG-tagged (DYKDDDDK) SUR2B were first cloned into pShuttle vectors and then the AdEasy vector (Stratagene), and packaged into recombinant adenoviruses in HEK293 cells according to manufacturer’s instructions63,64 ...
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bioRxiv - Biochemistry 2020Quote: ... fulgidus Csx3 (Supplementary figure 1) were generated using the QuikChange Site-Directed Mutagenesis kit as per manufacturer’s instructions (Agilent Technologies) and purified as for the wild-type protein.
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bioRxiv - Biochemistry 2020Quote: ... The membrane produced following transfer was probed with MAb214 primary antibody and then visualized with anti-mouse horseradish peroxidase secondary antibody (1:1000; Dako) for 1 h.
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bioRxiv - Neuroscience 2021Quote: ... A corresponding anti-rabbit or anti-mouse horseradish peroxidase (HRP)-conjugated secondary antibody at a dilution of 1:7000 (Dako) or 1:2000 (Pierce ...
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bioRxiv - Neuroscience 2020Quote: ... Free-floating sections (50 μm thickness) were prepared by a microslicer (DTK-1000, Dosaka EM) and treated with 1 mg/mL pepsin (DAKO) in PBS containing 0.1% Triton X-100 and 0.2 N HCl (Wako ...
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bioRxiv - Microbiology 2020Quote: ... an optimal mutation rate (0.3–1 base/kb) for 1µg of template was adopted as recommended in GeneMorph II Random Mutagenesis kit (Agilent Technologies). The PCR products were then digested with EcoRI and HindIII and ligated into the pJF118EH vector using the same restriction enzymes ...
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bioRxiv - Microbiology 2020Quote: Proteins were detected using the following primary antibodies: anti-enterovirus VP1 clone 5-D8/1 antibody purchased from Dako (Denmark); Mouse anti-Ub ...
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bioRxiv - Microbiology 2020Quote: ... 1:5,000 anti-mouse (for anti-FLAG) or 1:5000 anti-rabbit (for all the others) linked to peroxidase (Dako Agilent), and vizualized thanks to Clarity™ Western ECL substrate chemiluminescence reagent (BioRad ...
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bioRxiv - Microbiology 2020Quote: ... 1:5,000 anti-mouse (for anti-FLAG) or 1:5000 anti-rabbit (for all the others) linked to peroxidase (Dako Agilent), and vizualized thanks to Clarity™ Western ECL substrate chemiluminescence reagent (BioRad ...
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bioRxiv - Microbiology 2021Quote: ... Tissue samples were stained with hematoxylin and eosin or immunostained to detect SARS-CoV-2 antigen using VIDO-InterVac’s polyclonal rabbit anti-SARS-CoV-2 AS20-014 (1:400 dilution) and detected using an HRP-labelled polymer detection reagent (EnVision+ System – HRP Labelled Polymer, Agilent Technologies Canada Inc. ...