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Citations for Agilent :
2151 - 2200 of 2480 citations for Ataxin 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... quenched samples were passed through a co-immobilized pepsin/fungal XIII protease column (1mm × 2cm) at 50 µl min−1 and the digested peptides were captured on a 1mm × 1cm C8 trap column (Agilent) and desalted ...
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bioRxiv - Biochemistry 2024Quote: ... For all experiments the TIMS elution voltages were calibrated by known 1/K0 values from at least two out of three ions from Agilent ESI LC/MS tuning mix (m/z ...
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bioRxiv - Biochemistry 2024Quote: ... The signals were obtained with 8 cm-1 spectral resolution (unless specified otherwise) by performing 35 consecutive readings per measurement in 4,000-650 cm-1 range using MicroLab PC software (Agilent Technologies) and further processed with Spectragryph (F ...
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bioRxiv - Cell Biology 2024Quote: Cell proliferation rates were measured with label-free high-contrast brightfield microscopy in 6-well plates using Cytation 1 Cell Imaging Multi-Mode Reader (BioTek Instruments, Agilent) as described previously 58 ...
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bioRxiv - Cell Biology 2024Quote: hAC were fixed with 4% formalin and blocked for 1 h at 37°C (Protein-Block serum free, X0909 Dako Agilent). COL2 antibody (MA5-13026 ...
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bioRxiv - Immunology 2024Quote: ... residues D141 of Regnase-1 and D252 of Regnase-3 were mutated to asparigines using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent) following manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... The membranes were washed and incubated at room temperature for 1 h with rabbit anti-mouse IgG (Agilent, P026002-2) or goat anti-rabbit IgG (Agilent ...
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bioRxiv - Immunology 2024Quote: ... and MØ cell culture media was replaced with FCS-and bicarbonate-free DMEM medium supplemented with 4.5 mg ml-1 D-glucose and 2 mM glutamine (Agilent, USA) for another 60 min incubation at 37°C without CO2 ...
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bioRxiv - Genomics 2024Quote: ... We selected for guide integration with 2µg/ml puromycin and then induced prime editor expression with 1 µM doxycycline and determined editing rates by visualizing amplicons (Supplementary Table 2) on the bioanalyzer (Agilent).
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bioRxiv - Microbiology 2024Quote: ... Library size was validated by using Fragment Analyzer 48-Capillary Array with the HS NGS Fragment Kit (1-6000bp) (Agilent). Libraries were sequenced with a Nextseq 2×150 pb paired-end reagent kit (NextSeq 500/550 High Output Kit v2.5 ...
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bioRxiv - Cell Biology 2024Quote: ... of WRN was amplified by PCR from the pCMV-FlagWRN plasmid or from the pCMV-FlagWRNS1133D mutant The PCR product were subsequently purified and sub-cloned into pGEX4T-1 vector (Stratagene) for subsequent expression in bacteria as GST-fusion proteins ...
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bioRxiv - Biophysics 2021Quote: ... The thermal variations were in the range 25-95°C in a stepwise increment of 1 °C/minute step and using a 96-well PCR plate (Agilent Technologies) sealed with optical quality sealing tape (Microseal® ‘B’ seal from BIO-RAD) ...
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bioRxiv - Bioengineering 2021Quote: ... 10-20 µl injection volume depending on protein concentration) were injected on a Poroshell 300SB-C8 column (5 µm, 300Å, 1×75mm IDxL; Agilent Technologies) at a flow rate of 100 µl/min using solvent A (0.1% formic acid and 0.05% trifluoroacetic acid in water ...
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bioRxiv - Cancer Biology 2021Quote: ... was used to synthesize cDNA from 1 ug RNA and Brilliant III Ultra-Fast SYBR Green QPCR Master Mix (Agilent Technologies) was used for the Quantitative RT-PCR reactions ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by 1 hour incubation with secondary antibodies diluted in 2.5% milk in TBST (1:5000, goat anti-rabbit HRP, or goat anti-mouse HRP, DAKO, #P0448 & #P0447). Membranes were washed 3 times with TBST and exposed to HRP substrate (Immobilon Crescendo ...
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bioRxiv - Microbiology 2020Quote: The quality of purified libraries was analyzed on a Bioanalyzer High Sensitivity DNA chip (Agilent; see Figure 1 for an example) by following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... The following secondary antibodies were added for a 1-hour incubation at room temperature: anti-mouse IgG HRP-linked (Dako, p0447) and anti-rabbit IgG HRP-linked (Cell signaling ...
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bioRxiv - Cell Biology 2020Quote: 3000 control or p107KO cells or p107KO cells 24 hours post transfection with pCMV6-OCT-HA-eGFP alone or together with p107fl or p107mls were seeded in DMEM (Wisent) containing 10% FBS and 1% penicillin streptomycin on microplates (Agilent Technologies) and treated according to the required experiment ...
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bioRxiv - Immunology 2021Quote: Total IgG from individual donors was purified from ∼1 µL plasma using the AssayMAP Bravo platform (Agilent Technologies, Santa Clara, USA) with Protein G-coupled cartridges as described elsewhere (Larsen et al. ...
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bioRxiv - Neuroscience 2020Quote: ... Neurons were treated with AEVs from AD and normal participants for 1 hour and then subjected to fluorescent immunolabelling for MAC using a mouse anti-human C5b-9 antibody (1 μg/mL; RRID: AB_2067162, cat. no. M0777, Dako, Carpinteria, CA) targeting an epitope in the poly-C9 component of MAC in association with C5b ...
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bioRxiv - Developmental Biology 2022Quote: ... we washed the cells twice with 1X PBS and incubated them with FITC-conjugated anti-mouse IgG (dilution 1:500; Dako Cytomation) for 1 hour ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were then incubated overnight at 4°C with primary polyclonal anti-GFAP rabbit antibody (1:1000; Z0334, DAKO, Golstrup, Denmark) or anti-collagen IV rabbit antibody (1:200 ...
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bioRxiv - Cancer Biology 2019Quote: ... These cells were then incubated for 1 h in the absence of CO2 and transferred to the Seahorse XF96 extracellular flux analyzer (Seahorse Bioscience). If present ...
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bioRxiv - Neuroscience 2019Quote: ... The GFP probe was generated from a 733-bp fragment corresponding to nucleotides 733-1465 of the phrGFP II-1 mammalian expression vector (Agilent Technologies). The npba and npbb probes used were the same as those used for single in situ hybridization ...
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bioRxiv - Genomics 2019Quote: ... 1 μL of final RNA library was loaded on a Bioanalyzer 2100 DNA High Sensitivity chip (Agilent Technologies, Santa Clara, CA). Kapa Library Quantification Illumina/ABI Prism Kit protocol (KAPA Biosystems ...
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bioRxiv - Genetics 2019Quote: ... All qRT-PCR experiments were performed on diluted cDNA (1:5 in nuclease-free water) using the Brilliant II SYBR Green qPCR Master Mix (Agilent Technologies) and the 7900HT Real-Time PCR system (Applied Biosystems) ...
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bioRxiv - Cell Biology 2019Quote: ... anti-H4K20me2 The following secondary antibodies were used for western blot experiments: peroxidase-conjugated goat anti-rabbit (P448 DAKO, 1:2000) and goat anti-mouse (P447 DAKO ...
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bioRxiv - Neuroscience 2020Quote: ... The quality of the libraries is determined using the Standard High sensitivity NGS Fragment analysis kit (DNF-474, 1-6000 base pair) on the Agilent Fragment analyzer (Agilent, USA), yielding approximately 260 bp size fragments ...
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bioRxiv - Plant Biology 2020Quote: ... RT-PCR was performed with 1μL of diluted cDNA (1:9) in a Brillant III ultra fast SYBRgreen QPCR master mix (Agilent Technologies, USA) using a StepOnePlus Real-Time PCR System (Applied biosystems ...
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bioRxiv - Bioengineering 2020Quote: ... the sections were incubated with the secondary antibody (goat anti mouse IgG HRP, DAKO P0447, 1:100, EnVision+; System-HRP anti-rabbit DAKO, K4003) for 60 min at RT and stained using DAB peroxidase substrate solution (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... and were then incubated in a solution containing mouse antibody anti-Aβ (clone 6F/3D; 1:50, Dako M0872, Glostrup, Denmark). The sections were then processed with a secondary biotinylated horse anti-mouse IgG antibody (1:200 ...
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bioRxiv - Biophysics 2020Quote: ... Human SHP-1 with an N-terminal 6x His tag was produced in Escherichia coli BL21-CodonPlus (DE3)-RIPL strain (Agilent Technologies) and purified on Ni2+-NTA agarose (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... and then diluted to 1 ng/uL before checking insert size on an Agilent Technologies 2100 Bioanalyzer (Agilent Technologies; Waldbronn, Germany) and quantifying to greater accuracy by quantitative Q-PCR (library molarity >2 nM) ...
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bioRxiv - Systems Biology 2021Quote: ... using an Aminex HPX-87H ion-exchange column operated at 60°C with 5 mM H2SO4 as the mobile phase with a flow rate of 0.6 mL min-1 (Agilent, Santa Clara). The OD660 was measured with a Jenway 7200 spectrophotometer (Jenway ...
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Unraveling the functions of uncharacterized transcription factors in Escherichia coli using ChIP-exobioRxiv - Systems Biology 2021Quote: ... The extent of shearing was monitored with a 1% agarose gel and confirmed by separation on a 2100 High sensitivity Bioanalyzer chip (Agilent Technologies) upon completion of the immunoprecipitation ...
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bioRxiv - Biochemistry 2020Quote: ... The combined organic extract was concentrated to ~1 mL prior to clean-up by SPE (Agilent Bond Elut SI, 500 mg). The SPE columns were conditioned with hexane and the extract eluted with 6 mL 10% dichloromethane in hexane ...
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bioRxiv - Neuroscience 2020Quote: ... and then diluted to 1 ng/µl before checking insert size on an Agilent Technologies 2100 Bioanalyzer (Agilent Technologies; Waldbronn, Germany) and quantifying to greater accuracy by quantitative Q-PCR (library molarity >2 nM) ...
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bioRxiv - Microbiology 2020Quote: ... The secondary antibodies used were as follows: HRP-conjugated goat anti-mouse (1:5,000 dilution, Dako Agilent, Santa Clara, CA, USA), HRP-conjugated rabbit anti-hamster (1:2000 dilution ...
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bioRxiv - Microbiology 2020Quote: ... The secondary antibodies used were as follows: HRP-conjugated goat anti-mouse (1:5,000 dilution, Dako Agilent, Santa Clara, CA, USA), HRP-conjugated rabbit anti-hamster (1:2000 dilution ...
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bioRxiv - Molecular Biology 2022Quote: ... we created pcDNA3-AhR-1(LBD)-VP16 by site-directed mutagenesis of the pcDNA3-AhR-1-VP16 plasmid with the QuikChange II Site-Directed Mutagenesis Kit (Agilent, 200523). The following primer pair was used to create an L to A substitution at L363 ...
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bioRxiv - Neuroscience 2022Quote: ... Plasma (50 μL) was diluted by adding 150 μL of Agilent buffer A (1:4 dilutions, as recommended by Agilent Technologies), then filtered using a 0.45μm spin filter (Spin-X centrifuge tube filter ...
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bioRxiv - Cell Biology 2022Quote: ... Protein immunodetection was performed with primary abs diluted in blocking solution during overnight incubation at 4°C: rabbit polyclonal ab against alpha-1-antitrypsin (A0012, Dako, Agilent) at a 1:500 dilution ...
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bioRxiv - Cell Biology 2022Quote: ... Arrays were then washed with probing buffer (0.1% Tween 20, 1% BSA, 10% PBS) and incubated for 1.5 h in a hybridization chamber (Agilent, Santa Clara, CA) in a reaction mixture containing 80 μg of purified His-SETD6 in probing buffer in a total reaction volume of 950 μl ...
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bioRxiv - Neuroscience 2021Quote: ... and the membrane was blocked with 5% milk in tris-buffered saline with Tween 20 (TBST) before immunodetection with the following primary antibodies: tau (1:1000, DAKO, Denmark), pTau-S396 (1:500 ...
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bioRxiv - Plant Biology 2021Quote: ... samples by using modified CTAB and LiCl method [24,25] The quality of isolated RNA measured by UV spectrophotometer at 260/280and 260/230 nm wavelengths and 1% agarose gel electrophoresis followed by measuring RNA concentration using a 2100 Bioanalyzer (Agilent Technologies). The concentration of RNA was obtained in SaSHc 1460.90 ng/μl and in SaSLc 12.65 ng/μl ...
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bioRxiv - Molecular Biology 2021Quote: ... approximately 8 µg of protein was injected on a Zorbax 300SB-C18 column (5 µm, 300Å, 1×250mm IDxL; Agilent Technologies) and separated using a 30 min gradient from 5% to 80% solvent B at a flow rate of 100 µl/min (solvent A ...
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bioRxiv - Cancer Biology 2021Quote: ... W503F (PBD, polo-box domain 1) and H629A, K631M (PBD, polo-box domain 2) were generated by site-directed mutagenesis (Agilent Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... The PmeI-linearized pShuttle-vectors carrying different cDNA fragments were introduced into the replication-deficient adenoviral vector pAdEasy-1 through homologous recombination in BJ5183 cells (Agilent Technologies). Successfully recombined adenoviral vector was verified by sequencing ...
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bioRxiv - Physiology 2019Quote: ... Immunoblotting of 1-dimension SDS using COX7A2L antibody were analyzed by the ECL detection system using polyclonal goat anti-rabbit (Dako, P0448) as the secondary antibody.
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bioRxiv - Bioengineering 2019Quote: ... The microtissues were then washed in PBS (3 × 1 hour) before mounting on glass slides using fluoro-safe mounting media (Dako, S3023). Mounted samples were allowed to cure for 1 day and then imaged using a confocal microscope (Zeiss LSM700 Confocal Microscope).