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Citations for Agilent :
1851 - 1900 of 2480 citations for Ataxin 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... 1 × 105 CUTLL1 were plated per well on poly-D-lysine coated Seahorse XFe96 microplates (Agilent #101085-004) in XF RPMI medium (Agilent #103576-100 ...
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bioRxiv - Cell Biology 2024Quote: ... MONX10283) followed by goat-anti-rabbit or goat-anti-mouse conjugated to horseradish peroxidase (1:2000, Dako, P0448) and then visualized (VS200 slide scanner ...
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bioRxiv - Microbiology 2020Quote: ... Sections were then incubated with the primary antibody (diluted at 1 µg/ml in Antibody Diluent [Dako, Carpinteria, CA]) for 30 min at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... 0.1% (v/v) TFA as mobile phase on a 4.6 mm x 250 mm Zorbax 300SB-C8 column (Agilent) at 80 °C column temperature and 1 ml min-1 flow rate (Supplementary Fig ...
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bioRxiv - Biophysics 2020Quote: ... Optical absorption spectra of the samples in a 300-900 nm range were recorded within about 10 s at given time intervals (about 0.3-1.0 min) in a 3 mL quartz cuvette (Helma QS1000, 1 cm pathlength) using a Cary 60 spectrometer (Agilent). Alternatively ...
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bioRxiv - Cancer Biology 2021Quote: The appropriate pGEX-6P-1 constructs were transformed into the chemically competent BL21-Codon Plus (DE3)-RIPL (Agilent Technologies) E ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were washed three times with PBS containing 0.05% Tween-20 and incubated for 1 h with horseradish peroxidase (HRP)-conjugated secondary antibodies (DAKO) at a dilution of 1:10,000 ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μl of this solution was subjected to TapeStation RNA ScreenTape Analysis (Agilent #5067-5576, #5067-5577, #5067-5578) to detect ssDNA ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA was purified using 1.8:1 AMPure XP Beads: DNA ratio and libraries were quantified and sized on a D1000 Tapestation tape (Agilent). Libraries ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were then incubated for 1 hour at 37°C in atmospheric CO2 in pH 7.4 unbuffered RPMI1640 with 1.67 mM glucose (Seahorse bioscience). Islets were first stimulated with high glucose (16.7 mM ...
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bioRxiv - Developmental Biology 2021Quote: ... DAPI (1:500, Dojindo) was used for nuclear staining and the sections were mounted with fluorescence mounting medium (Dako). Fluorescent images were captured using an all-in-one fluorescence microscope (BZ-X700 ...
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bioRxiv - Immunology 2022Quote: ... the membrane was incubated in secondary antibody solutions (TBS containing 0.1 % Tween-20 and 5 % BSA, HRP-conjugated secondary antibodies (1:5000, Dako)) at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies used were rabbit anti-cow glial fibrillary acidic protein (GFAP; 1:5000; Z0334; Dako, Carpinteria, CA, USA) to detect astrocytes ...
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bioRxiv - Immunology 2022Quote: ... CF (R&D/BT10500-050) was incubated at a 4:1 molar ratio with either streptavidin-PE (Prozyme PJRS25) or streptavidin-APC (Prozyme PJ27S ...
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bioRxiv - Genomics 2020Quote: Twenty µg of high molecular weight (HMW) DNA (50 ng µl-1) was evaluated on a Fempto Pulse (Agilent) with a three hour run time and converted to SMRTbell® libraries using SMRTbell® Express Template Prep kit according to the manufacturer instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Site mutagenesis was performed on pCMV4 CYP2D6*1 plasmid23 with QuikChange II Site-Directed Mutagenesis Kit (Agilent, CA, US). Plasmid cDNA encoding variants with the following amino acid exchanges were created ...
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bioRxiv - Neuroscience 2019Quote: ... The blots were washed again and an alkaline-phosphate-conjugated goat anti mouse antibody was added (DAKO, 1:2000). Another washing step with TBST and B3 buffer (100 mM Tris ...
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bioRxiv - Developmental Biology 2019Quote: ... DAPI (1:500, Dojindo) was used for nuclear staining and the sections were mounted with fluorescent mounting medium (Dako). At least three embryos of each genotype were used for each analysis.
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bioRxiv - Plant Biology 2019Quote: ... Total RNA quality was examined on 1% agarose gels and RNA Nanochips on an Agilent 2100 Bioanalyzer (Agilent Technologies). Samples with RNA integrity number ≥ 7.0 were used for library preparation ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... MG and TA99 were used at a concentration of 1µg/ml together with a rabbit anti-human Von Willebrand Factor (1:800, Dako) in 3% BSA in PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... The resulting 40 samples were diluted 4:1 with Buffer A for Multiple Affinity Removal LC Columns (Agilent Technologies), filtered through a 0.22 μm hydrophilic PVDF membrane filter plate (Millipore) ...
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bioRxiv - Neuroscience 2020Quote: ... with 5% BSA and 0.4% triton X-100) followed by incubation overnight at 4°C with 1:500 rabbit anti-C3 antibody (Dako) and 1:10,000 guinea pig anti-vGluT2 (Synaptic Systems ...
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bioRxiv - Cancer Biology 2021Quote: ... Tissues next underwent antigen retrieval by submerging sides in 3-in-1 Target Retrieval Solution (pH 9, DAKO Agilent) and incubating at 97°C for 40 minutes in a Lab Vision PT Module (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: ... Tissues next underwent antigen retrieval by submerging sides in 3-in-1 Target Retrieval Solution (pH 9, DAKO Agilent) and incubating at 97°C for 40 minutes in a Lab Vision PT Module (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... incubated with DAPI (1:10’000) to label nuclei for 10 min and mounted using Fluorescence mounting medium (Dako, S3023). Fluorescent images were recorded with a Leica SP5 Confocal Microscope.
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bioRxiv - Neuroscience 2020Quote: ... and followed by incubation with primary antibodies or negative isotype controls diluted in blocking buffer for 1 hour (Covance PRB-278P rabbit polyclonal IgG anti-PAX6, 10μg/mL; Dako Z0334 rabbit polyclonal IgG anti-GFAP ...
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bioRxiv - Neuroscience 2021Quote: ... Nuclei staining with Hoechst (1:10.000 in PBS) applied between initial washes or with ProLong Gold DAPI mounting medium (Dako). Micrographs were recorded using a Zeiss LSM710 point laser (Argon Lasos RMC781272 ...
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bioRxiv - Cancer Biology 2019Quote: ... This was followed by sequential 1-hour incubations with the secondary antibodies (En Vision+ System-HRP-Labeled Polymer; Dako) and visualization with the Liquid DAB+ Substrate Chromogen System (Dako) ...
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bioRxiv - Neuroscience 2020Quote: ... was generated by site directed mutagenesis using primers oM18_17 and oM18_18 (Table 1) and a Quikchange II Site-directed Mutagenesis kit (Agilent, 200523). The PCR product was digested with DpnI enzyme to remove template vector and transformed into Stbl2 chemically competent cells (Thermo Fisher ...
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bioRxiv - Physiology 2021Quote: ... Samples were incubated overnight at 4°C in primary antibodies targeting anti-insulin (1:200, Abcam #Ab7872, Dako #A0564), anti-glucagon (1:100 ...
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bioRxiv - Bioengineering 2021Quote: ... and cDNA library size was checked using Fragment Analyzer HS NGS Fragment Kit (1-6000bp) (Agilent formerly Advanced Analytical). Only high-quality cDNA libraries without notable degradation were used for sequencing library construction ...
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bioRxiv - Biochemistry 2020Quote: ... The RNA concentration and quality were assessed using the Advanced Analytical Fragment Analyzer (Agilent Technologies, CA, USA, table 1) prior to library construction ...
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bioRxiv - Biochemistry 2020Quote: ... equipped with a DB-1 MS capillary column (15 m x 0.25 mm x 0.25 μm, Agilent 122-0112). Chromatography was conducted with helium gas ...
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bioRxiv - Plant Biology 2020Quote: ... The powder (1 g) was immediately transferred to a 20-mL head-space vial (Agilent, Palo Alto, CA, USA) containing 2 mL NaCl-saturated solution to inhibit any enzyme reaction ...
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bioRxiv - Systems Biology 2021Quote: ... and a mass accuracy of <1 ppm was often achieved using the standard tuning mixture (G1969-85000, Agilent Technologies). The m/z scan range was 100−2000 ...
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bioRxiv - Immunology 2021Quote: Synthesised antigen constructs were transformed into competent XL-1 Blue Escherichia coli cells (Agilent Technologies, Santa Clara, CA, USA) by electroporation ...
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bioRxiv - Cancer Biology 2021Quote: ... Secondary antibodies and dilutions used were as follows: anti-Mouse Immunoglobulins/FITC (1:400, F0479, Dako, Carpinteria, CA, USA) and anti-Rabbit IgG (H+L ...
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bioRxiv - Cell Biology 2021Quote: ... Single cells were separated using a 100 μm strainer and labelled with CD31 antibody (1:200, M082329-2, DAKO) at 4°C for 45 min followed by 30 min staining with a goat anti-mouse secondary antibody conjugated to AlexaFluor 488 or 555 (1:400 ...
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bioRxiv - Biophysics 2020Quote: ... in 20 min at a flow rate of 0.4 mL min-1 using a Zorbax Narrow Bore (2.1×50 mm, 5 μm) C18 column (Agilent Technologies).
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bioRxiv - Cancer Biology 2021Quote: ... Primary Axl antibody (RnD Systems, AF154) was diluted 1:400 in Antibody Diluent with Background Reducing Components (Dako, S3022). 200 µL of antibody solution was put on each slide to cover all TMA specimens and incubated overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: Secondary antibodies used were Polyclonal Goat Anti-Rabbit or Polyclonal Rabbit Anti-Mouse (1:1000) HRP-conjugated (Dako Agilent), or IRDye® 680RD (1:20,000)- or 800CW (1:10,000)-conjugated for quantitative fluorescence measurements on an Odyssey® Fc Dual-Mode Imaging System (LICOR Biosciences) ...
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bioRxiv - Cell Biology 2021Quote: Secondary antibodies used were Polyclonal Goat Anti-Rabbit or Polyclonal Rabbit Anti-Mouse (1:1000) HRP-conjugated (Dako Agilent), or IRDye® 680RD (1:20,000)- or 800CW (1:10,000)-conjugated for quantitative fluorescence measurements on an Odyssey® Fc Dual-Mode Imaging System (LICOR Biosciences) ...
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bioRxiv - Immunology 2022Quote: ... and a tyramide-barcode staining cycle performed for mouse anti-MUM1 primary antibody (1:100 dilution, clone MUM1p, Agilent Dako Products ...
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bioRxiv - Immunology 2022Quote: ... coated XFe96 plate with fresh XF media (Seahorse XF RPMI medium containing 10 mM glucose, 2 mM L-glutamine, and 1 mM sodium pyruvate, PH 7.4; all reagents from Agilent). Basal OCR and ECAR were measured in the presence of Oligomycin (1.5 μM ...
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bioRxiv - Cancer Biology 2022Quote: ... The primary antibodies were followed by respective HRP-conjugated anti-mouse or anti-rabbit secondary antibody (1: 2000; Dako). Detection of peroxidase activity was performed by using the Amersham ECL western blot detection kit (GE Healthcare).
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bioRxiv - Genetics 2022Quote: ... followed by incubation with goat anti-rabbit IgG conjugated to horseradish peroxidase (HRP) (1:2000, Agilent Cat# P0448, RRID:AB_2617138). Ubiquitin was detected using mouse monoclonal anti-ubiquitin antibody (1:500 ...
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bioRxiv - Bioengineering 2022Quote: ... retinal cross-sections were treated with antigen retrieval solution at 99 °C for 1 h (PH 6.1; Dako #S1699) and washed in 1x PBS ...
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bioRxiv - Neuroscience 2020Quote: Primary antibodies used were listed as follows: rabbit anti-GFAP (a marker for astrocytes, 1:1000, Cat# Z0334, DAKO), rabbit anti-NeuN (a marker for neurons 1:1000 ...
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bioRxiv - Microbiology 2021Quote: Plasmids encoding the single-mutation variants found in P.1 and 10-mutation variant (BZΔ10) were generated by Quikchange II XL site-directed mutagenesis kit (Agilent). Recombinant Indiana VSV (rVSV ...
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bioRxiv - Molecular Biology 2020Quote: ... XL10-Gold ultracompetent cells (Tetr Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac Hte [F’proAB lacIqZΔM15 Tn10 (Tetr) Amy Camr] (Agilent Technologies) were used.