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Citations for Agilent :
1801 - 1828 of 1828 citations for Recombinant Human Ribulose 5 Phosphate 3 Epimerase His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2019Quote: The MBP-5-HT3A-ICD-pMALX soluble fusion proteins were overexpressed in Escherichia coli (E. coli) BL21-CodonPlus (DE3)-RIPL cells (Agilent Technologies; Santa Clara, CA). The cells were harvested by centrifugation (5,000 g for 15 min at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... linear gradient from 5% to 35% ACN in 50 mM TEAAc pH 7 over 15 min at 50 °C, Agilent Technologies Series 1200 HPLC). The collected fractions were freeze dried 3 times ...
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bioRxiv - Synthetic Biology 2020Quote: LC-MS analysis was performed with the ZORBAX SB-Aq StableBond Analytical column (5 μm, 4.6 × 250 mm, Agilent Teologies, Santa Clara, CA, USA) on an Agilent 1260/6460 Triple-Quadrupole LC/MS system (Santa Clara ...
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bioRxiv - Immunology 2021Quote: ... TMT-labelled peptides were fractionated using high pH reverse phase chromatography on a C18 column (150 × 2.1 mm i.d. - Kinetex EVO (5 μm, 100 Å)) on a HPLC system (Agilent, LC 1260 Infinity II, Agilent). A two-step gradient was applied ...
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bioRxiv - Physiology 2022Quote: ... The first was a DB 5 column (20 m long x 0.18 mm internal diameter x 0.18 μm thick) (Agilent J & W Scientific, Folsom, CA, USA), and the second a Rxi-17 column (0.84 m long x 0.1 mm internal diameter x 0.1 μm thick ...
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bioRxiv - Microbiology 2023Quote: ... the GC-MS analysis was performed with An OPTIMA 5 MS Accent fused-silica capillary column on Agilent7890A/5975C GC-MS system (Agilent Technologies Inc., CA, USA), while the LC-MS analysis was performed on a ThermoFisher Ultimate 3000 UHPLC system with a Waters ACQUITY UPLC BEH C18 column (2.1mm × 100 mm ...
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bioRxiv - Cell Biology 2024Quote: ... 2.5 mM calcium chloride for 15 min at room temperature prior to recording fluorescence on a Citation 5 microplate reader (Agilent-BioTek, Santa Clara, CA), exciting at 488 nm and measuring emission at 535 nm ...
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bioRxiv - Bioengineering 2024Quote: ... The diameter of cells adhered to TCP was measured by the Cytation 5 cell imaging multimode reader measurement tool (Agilent Technologies, Santa Clara, CA). In-suspension measurements were determined using the T4 Auto Cellometer Bright Field Cell Counter (Nexelom Bioscience ...
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bioRxiv - Biochemistry 2022Quote: ... coupled with automated fraction collector in batches through a continuous gradient on an Agilent ZORBAX SB-C18 column (Agilent, 5 μm, 4.6 × 250 mm) at a column temperature of 40 °C to yield 45 fractions based on retention time and one fraction was collected per minute ...
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bioRxiv - Plant Biology 2023Quote: ... Aliquot of 5 μl of the eluate was injected into the LCMS system consisting of UHPLC 1290 Infinity II (Agilent, Santa Clara, CA, USA) coupled to 6495 Triple Quadrupole mass spectrometer (Agilent) ...
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bioRxiv - Cancer Biology 2024Quote: ... The analytes were separated on a Sciex C18 column (4.6 mm × 150 mm, 5 μm) maintained at 50°C using a 1260 Infinity HPLC instrument (Agilent Technologies, Santa Clara, CA, USA). A gradient flow of the mobile phase was applied ...
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bioRxiv - Microbiology 2024Quote: Cell extracts were analyzed by injecting 5 µL into an Agilent 1290 Infinity II HPLC with an Agilent 6495C iFunnel QQQ mass spectrometer for detection (Agilent, Santa Clara, CA, USA). Separation of the analytes was achieved on an Agilent (Santa Clara ...
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bioRxiv - Synthetic Biology 2020Quote: ... Fractions containing the target peaks were combined and further purified by a Reveleris flash chromatography system (Grace) using a C18 Preparative column (Agilent, 5 μm, 21.2 × 150 mm). A gradient method (55% acetonitrile-water to 85% acetonitrile-water in 12 mins ...
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bioRxiv - Neuroscience 2019Quote: ... Paraffin embedded human brain sections were de-waxed for antigen retrieval with 90% formic acid (5 min) followed by citrate boiling (45min, 98°C DAKO Citrate Buffer, DAKO PT Link). Sections were then blocked using 0.1% (w/v ...
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bioRxiv - Biochemistry 2020Quote: ... were separated by a gradient elution from 5% solvent B to 95% solvent B over 15 min on a high-capacity nano-LC chip (Agilent Technologies; part no. G4240-62010) driven by a 1200 series nano-flow HPLC system (Agilent ...
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bioRxiv - Synthetic Biology 2022Quote: ... Further purification of the target product was performed using a reverse-phase HPLC semi-preparative column (Agilent Zorbax SB C18, 250 × 9.4 mm, 5 μm) to collect the target compound manually ...
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bioRxiv - Microbiology 2019Quote: ... Fractions containing the target compound were combined for further purification using a semi-prep HPLC with a C18 column (Agilent, 5 μm, 21.2 × 150 mm).
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bioRxiv - Microbiology 2022Quote: ... The resulting membrane was blocked for 1 hr in PBS + 5% BSA (Fisher Bioreagents, BP1605-100) before being incubated overnight with primary antibodies [anti-VP1 (Dako, anti-enterovirus clone5-D8/1) or anti-eIF4G (Cell Signaling Technology ...
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bioRxiv - Biochemistry 2024Quote: ... and the molar mass distribution in PVC materials were determined using a 1260 HPLC system equipped with three PLgel 5 µm Mixed-C columns and a PLgel guard column (Agilent Technologies, Santa Clara, CA, USA). Approximately 5 mg of PVC was dissolved in 1 mL of tetrahydrofuran at room temperature for at least 14 h ...
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bioRxiv - Cancer Biology 2024Quote: ... Blocking was performed in 0.03% H2O2 containing sodium azide for 5 minutes and primary antibodies (Table S1) were incubated for 60 minutes before detection (Dako EnVision+ System-HRP labeled Polymer) for 30 minutes and development for 5 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... The cytolysis of A549 cells was monitored over a period of 5 days using xCELLigence® RTCA eSight device (Agilent Technologies, Inc., Santa Clara, CA) by impedance.
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bioRxiv - Molecular Biology 2023Quote: ... Liquid chromatography electrospray ionization mass spectrometry (LC-ESI-MS) was performed using a C3 column (Agilent Poroshell 300SB-C3, 1 x 75 mm, 5 µm) with a gradient of 5% to 95% acetonitrile (0.1% v/v formic acid ...
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bioRxiv - Molecular Biology 2024Quote: ... in PBST for 1 h at RT Sections were incubated overnight at RT in primary antibody rabbit anti-GFAP (Agilent, Dako Z0334,1:500 in 5 % NGS-PBST) and then with biotinylated secondary antibody anti-rabbit IgG (H+L ...
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bioRxiv - Molecular Biology 2023Quote: We characterized the peptide samples using Liquid chromatography mass spectrometry (LCMS) with an Agilent 1260 system equipped with Single Quadrupole LC/MS (6120B) and a 5 μm C18 column (Agilent Zorbax Eclipse Plus, PN 959746-902). We injected the samples and subjected them to an elution gradient of 5-95% aqueous (0.1% FA ...
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bioRxiv - Molecular Biology 2024Quote: ... in PBST for 1 h at RT Sections were incubated overnight at RT in primary antibody rabbit anti-GFAP (Agilent, Dako Z0334,1:500 in 5 % NGS-PBST) and then with biotinylated secondary antibody anti-rabbit IgG (H+L ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... diethyl ether and purified on an Agilent 1200 series High-Performance Liquid Chromatography (HPLC) instrument (10–75% HPLC-grade acetonitrile for 20 minutes at a 2 mL/min flow rate over an Agilent Zorbax C18 column (5 µm, 9.4 x 250 mm) tracked at 220 ...
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bioRxiv - Biochemistry 2023Quote: The crude peptide mixture was purified using reverse-phase high performance liquid chromatography (RP-HPLC) on a semi-preparatory C18 column (Agilent, ZORBAX 300SB-C18, 9.4 x 250 mm, 5 μm) with an Agilent HPLC system (1260 Infinity II) ...
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bioRxiv - Bioengineering 2024Quote: ... samples were collected immediately after euthanizing the animals and subjected to radio-HPLC (Agilent 1260 Infinity II HPLC with Agilent column: 300SB-C18, 9.4 X 250 mm, 5 µm). The mobile phase started with 95% solvent A (0.1% TFA in water ...