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Citations for Agilent :
1501 - 1550 of 1828 citations for Recombinant Human Ribulose 5 Phosphate 3 Epimerase His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The HNO3 concentration was adjusted to 5% and the zinc concentration was determined by ICP-MS (Agilent7500cx, Agilent Technologies).
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bioRxiv - Plant Biology 2024Quote: ... The organic phase was dried using anhydrous MgSO4 and analysed on GC-2010 Pro gas chromatograph (Shimadzu, Japan) equipped with an HP-5MS 30 m × 0.25 mm (5%-Phenyl)-methylpolysiloxane column (19091S-133, Agilent) with nitrogen as the carrier gas with splitless injection mode ...
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bioRxiv - Physiology 2024Quote: ... live cells stained with Hoechst 33342 (1:2,000 dilution) (ThermoScientific, Cat#62249) were enumerated using Cytation 5 plate reader (BioTek Instruments - Agilent). Values obtained from the Mito-Stress test were normalized per 1,000 cells.
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bioRxiv - Cell Biology 2024Quote: ... Approximately 120 µg of solubilized membrane fractions were fractionated into 89 fractions using an äkta pure chromatography system (Cytiva) equipped with a Bio-SEC-5 1000Å analytical column (300×7.8 mm, Agilent) and a SEC-5 1000 Å guard column (50×4.6 mm ...
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bioRxiv - Physiology 2024Quote: ... Rehydrated 5-μm sections were stained with primary antibodies against insulin (dilution 1:1000; Dako, Santa Clara, CA, USA), somatostatin (dilution 1:300 ...
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bioRxiv - Molecular Biology 2024Quote: ... conducted using an Agilent 1100 Series HPLC and C18 column (Eclipse XDB-C18 5 μm, 4.6×150 mm (Agilent)) ...
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bioRxiv - Microbiology 2024Quote: ... with a ZORBAX SB-C18 column (particle size, 5 μm; 250 mm × 4.6 mm) (Agilent Technologies, Santa Clara, USA) and a UV diode array detector (Agilent Technologies ...
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bioRxiv - Biochemistry 2024Quote: ... The material was repeatedly injected into an analytical Eclipse XDB-C8 column (Agilent, 5 μm, 4.6 x 150 mm), operating at a flow rate of 0.5 mL/min with a mobile phase of H2O and MeCN (+0.1% TFA) ...
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bioRxiv - Bioengineering 2024Quote: ... Primary Cells were stained with DAPI and then imaged with a high throughput imaging system (BioTek Cytation 5, Agilent).
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bioRxiv - Microbiology 2023Quote: ... A large-inner diameter (i.d.) trapping column (300-μm i.d. x 5 mm, Agilent Technologies, Santa Clara, CA, USA) was coupled to the analytical column (75-μm i.d ...
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bioRxiv - Plant Biology 2023Quote: ... A gradient elution system was optimized with the two mobile solvents to separate metabolites on an Eclipse XDB-C18 analytical column (250 × 4.6 mm, 5 µM, 25 ◦C, Agilent). The gradient solvent system was composed of ratios of solvent A to B ...
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bioRxiv - Cancer Biology 2022Quote: ... The material was loaded on to a Zorbax 2.1 x 150 mm (5 µm particle size) Extend-C18 column (Agilent) for basic reverse-phase fractionation ...
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bioRxiv - Plant Biology 2023Quote: ... The LC separation was conducted on a Poroshell 120 EC-C18 analytical column (Agilent, 2.1 × 5 mm, 1.8 μm). Samples (1 μL ...
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bioRxiv - Systems Biology 2023Quote: ... pH 8.5) and loaded onto an Agilent 300 Extend-C18 column (5 μm, 4.6 mm × 25 cm; Agilent Technologies). The peptides were then resolved using an increasing gradient of solvent B (10 mM TEAB in 90% acetonitrile (ACN) ...
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bioRxiv - Microbiology 2023Quote: ... Using a LC-10AT HPLC instrument (Shimadzu), the pooled sample was loaded onto a ZORBAX Eclipse XDB C18 column (5 μm, 4.6 × 150 mm) (Agilent) that had been equilibrated with 25 mM sodium acetate ...
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bioRxiv - Cancer Biology 2023Quote: ... for 5 minutes and incubated with the primary antibody of interest which was prepared in antibody diluent (S0809, Dako). Slides were put in a humidified chamber at 4C overnight ...
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bioRxiv - Synthetic Biology 2023Quote: ... fitted with a guard column (Zorbax Eclipse XDB-C18, 2.1 x 5 mm 1.8 μm, Agilent part # 821725-903) using a 1290 Infinity II UHPLC (G7120AR ...
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bioRxiv - Cell Biology 2023Quote: ... The α10-N839Q mutation was generated by QuickChange mutagenesis kit following the instruction (cat# 200517-5, Agilent Technologies, Inc.). All DNA constructs were identified by sanger sequencing service provided by Retrogen ...
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bioRxiv - Physiology 2023Quote: ... during which lipids were separated on an Eclipse XDB C18 5-μm column (i.d. 150 × 4.6 mm; Agilent Technologies) using an isocratic mobile phase comprising chloroform:methanol (1:1 v/v ...
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bioRxiv - Biochemistry 2023Quote: ... Gly241Glu and Asp235Ala:Gly241Glu were introduced into the previously published vectors (5) using the QuickChange II Site-directed mutagenesis kit (Agilent) using primers listed in Table S2.
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bioRxiv - Biochemistry 2023Quote: ... Colonies were stained with Wright-Giesma and plates were scanned using the Biotek Cytation 5 Imaging Multimodal Reader (Agilent). From scanned images ...
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bioRxiv - Physiology 2023Quote: ... The purity of LCA probe was determined to be >95% by HPLC with UV detection at 220 nm (HD-C18 column, 5 μm, 4.6 × 250 mm, Agilent Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... used a two solvent gradient (Mobile Phase A: 10 mM Ammonium Acetate in 90/10 Water/Acetonitrile, pH 9.2, 5 μm Agilent InfinityLab Deactivator Additive ...
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bioRxiv - Developmental Biology 2024Quote: ... Point mutations were introduced into sel-5 and dpy-23 sequences using the QuikChange Site-directed Mutagenesis kit (Agilent). Transcriptional reporter Ppgp-12::gfp was created by inserting 3410 bp of pgp-12 promoter upstream of GFP in pPD95.81 ...
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bioRxiv - Physiology 2024Quote: ... Secreted protein in cell culture media was enriched using StrataClean Resin (Agilent, 10 µl per 5 ml of media) as previously described37 ...
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bioRxiv - Cancer Biology 2024Quote: ... The eluted peptides were then trapped in a reverse-phase column (Zorbax 300SB-C18, 0.3 × 5 mm; Agilent Technologies), and separated on a homemade column (HydroRP 2.5 µm ...
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bioRxiv - Developmental Biology 2024Quote: ... Mutations in the miRNA binding sites were introduced using the QuikChange II XL system (Agilent Technologies; CatNo. 200521-5). Primers used are shown in Table S2 ...
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bioRxiv - Biophysics 2024Quote: ... in xylene and deposited with a 12 μm inter-fiber spacing on top of large diameter (2 μm) support fibers prepared from 5 wt% solutions of polystyrene (MW: 15,000,000 g/mol, Agilent Technologies) dissolved in xylene ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then rinsed with PBS containing 1% FBS prior to imaging using a Cytation 5 instrument (Agilent BioTek). Mean fluorescence was calculated using Gen5 software (RRID:SCR_017317).
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Co-Metabolic Growth and Microbial Diversity: Keys for the depletion of the α, δ, β and γ-HCH isomersbioRxiv - Microbiology 2024Quote: ... internally coated with a 0.18 µm film composed of a non-polar stationary phase containing 5% diphenyl-polysiloxane (HP5-MS, Agilent). The injection was performed in split-less mode at 280°C ...
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bioRxiv - Microbiology 2024Quote: Content of the rRNA hydrolysate was resolved using Zorbax SB-C18 column (5 µm, 0.5 x 150 mm, Agilent) and the LC conditions were tuned empirically ...
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bioRxiv - Microbiology 2024Quote: ... cells were incubated for 30 minutes in 5 mM MβCD in fresh XFp medium (Agilent Technologies, Santa Clara, CA). To interrogate mitochondrial function ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were Hoechst-stained and relative cell numbers counted using a BioTek Cytation 5 Cell Imaging Multimode Reader (Agilent) at the specific timepoints.
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bioRxiv - Physiology 2024Quote: ... and injected 100 µL of sample into an Agilent 1100 series HPLC fitted with a Zorbax RX-SIL column (4.6x25 mm, 5 µm, Agilent). Elution of samples was achieved by a gradient mobile phase of 0.5% ethyl acetate in hexane for 5 minutes then increased to 10% ethyl acetate in hexane for 20 minutes ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... As species were separated by ion-pairing chromatography using a Zorbax SB-C18 column (150 mm × 4.6 mm, 5 μm particle size, Agilent) through isocratic elution for 25 min with the mobile phase used for extraction ...
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bioRxiv - Plant Biology 2024Quote: ... using a HP-5MSI 5% phenyl methyl silox with 30 m × 250 µM × 0.25 µm film thickness (Agilent Technologies). Identification and quantification were conducted using AMDIS (Automated Mass spectral Deconvolution and Identification System ...
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bioRxiv - Developmental Biology 2024Quote: ... The material was loaded on to a Zorbax 2.1 mm x 150 mm (5 µm particle size) Extend-C18 column (Agilent) for basic reverse-phase fractionation ...
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bioRxiv - Immunology 2022Quote: Naive CD3+ T cells were placed on PLL-coated Seahorse Bioanalyzer XFp culture plates (3 × 105 cells/well) with Seahorse XF RPMI Assay Media (RPMI Medium, pH 7.4, 103576-100; Agilent Technologies, Santa Clara, CA, USA), supplemented with 10 mM glucose (103577-100 ...
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bioRxiv - Cell Biology 2022Quote: ... were diluted with ultra-pure water to reduce the acid concentration below 3% and loaded into the ICP-MS-MS (triple quad Agilent 8800x ICP-MS-MS). The instrumental conditions were optimized to remove interferences by using a collision/reaction cell ...
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bioRxiv - Molecular Biology 2019Quote: ... We measured O2 consumption of fully differentiated adipocytes at day 7 with an XF24-3 extracellular flux analyzer (Agilent Technologies, Santa Clara, CA, USA). Basal respiration was also assessed in untreated cells.
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bioRxiv - Physiology 2020Quote: ... blocked with 3% BSA in PBS1 and incubated overnight at 4°C with primary polyclonal antibodies for glucagon and insulin (Dako, Agilent, Santa Clara, CA, USA) (Table S8) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... For phthalates and BPA extraction we used Agilent Vac Elut Manifold with SPE cartridge C18 ODS 3 ml tubes 200 mg (Agilent Santa Clara CA, USA). SPE cartridges were conditioned with 2 ml of methanol and then 2 ml of MilliQ water ...
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bioRxiv - Molecular Biology 2022Quote: ... amplified and labeled with Cyanine 3 (Cy3) as instructed by the manufacturer of the One-Color Agilent Low Input Quick Amp Labeling Kit (Agilent Technologies, Les Ulis, France). Cy3-labeled cRNA was hybridized onto Agilent Whole Human Genome Oligo 8×60K V2 Arrays (SurePrint G3 Human Gene Expression 8×60K v2 Microarray Kit ...
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bioRxiv - Synthetic Biology 2023Quote: Samples (3 µL) were injected into an ion mobility drift tube-quadrupole time-of-flight mass spectrometer (LC-IM-QTOF, Agilent 1290 series LC 6560). An Agilent Poroshell 120 HILIC-Z 150 × 2.1 mm ...
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bioRxiv - Neuroscience 2022Quote: ... samples were suspended in a 4M urea buffer containing ampholytes and subjected to off-gel IEF fractionation using a 24 well strip (pH 3-10) (Agilent, 3100 Off- gel fractionator). Following IEF ...
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bioRxiv - Neuroscience 2023Quote: ... 50,000 cells per well were plated after 3 days of induction in a specialized Seahorse analyzer 96-well plate (Agilent, cat. no. 103774-100). 10-12 day old i3Neurons were treated with 5 nM vincristine ...
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bioRxiv - Immunology 2024Quote: ... turbidity was monitored in 2-minute intervals at 600 nm absorption using a Cytation 3 plate reader (BioTek, Agilent Technologies, Santa Barbara, CA, USA) preheated to 37 °C and after equilibrating for 20 min ...
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bioRxiv - Plant Biology 2021Quote: ... Two µl of samples were introduced in the splitless injection mode in DB-5 (60 m × 0.25 mm, 1 µm film thickness, Agilent, CA) using helium as a carrier gas ...
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bioRxiv - Genetics 2021Quote: ... Analytes were separated on a HP-5 capillary column (50 m by 320 μm by 1.05 μm column; Agilent, USA). The carrier gas was helium (constant flow of 1.4 mL · min-1) ...
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bioRxiv - Biophysics 2020Quote: ... and the MFS0 peptide biotin-N-GGGGS (GPGGA)5 GGKYG GS-K(ε-N3) were purified via preparative C18 reverse phase HPLC (5 µm particle size, 250×21.2 mm, Agilent) applying a linear gradient of 0.1% triflouracetic acid (TFA ...