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Citations for Agilent :
1351 - 1400 of 1717 citations for Recombinant Human Ribulose 5 Phosphate 3 Epimerase His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... ITO slides were then stained for H&E and imaged using the Cytation 5 (Agilent, Santa Clara, CA) and Epsilon scanner at 3000 dpi to select regions of interest using the open-source program ...
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bioRxiv - Biochemistry 2023Quote: ... HPLC was performed on an Agilent 1260 infinity II, equipped with a C-18 column (Mackerey-Nagel, 5 µm, ref 760.100.40) using acetonitrile (HPLC grade, Agilent) and ddH2O (0.1% (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... at 5 mM for 2 h or by UV irradiation at 20 mJ/cm2 using Stratalinker 1800 (Stratagene) followed by 2 h incubation at 37 °C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Some of them were subjected to further purification using semipreparative techniques (Agilent, C18, 5 μm, 250 × 10 mm) using appropriately selected aqueous AcN or MeOH solvent systems.
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bioRxiv - Synthetic Biology 2024Quote: ... formic acid at a flow rate of 1.0 mL/min equipped with a ZORBAX SB-C18 column (4.6 × 250 mm, 5 μm, Agilent). Quantification was performed using standard calibration curves prepared using a series of known concentrations of the standard ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Zorbax SB300-C18/5 μm particles and SPEC-PTC18/SPEC-PTSCX cleanup pipette tips were purchased from Agilent Technologies (Santa Clara ...
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bioRxiv - Neuroscience 2024Quote: ... Phosphopeptide enrichment was performed on 5 µL phase Fe(III)– NTA cartridges on an AssayMAP Bravo platform (Agilent) following the immobilized metal affinity chromatography protocol ...
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bioRxiv - Cell Biology 2024Quote: ... the plate containing the follicles was transferred to a live-cell imaging system (Agilent BioTek Cytation 5, USA) to capture images at 6-minute intervals during ovulation ...
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bioRxiv - Bioengineering 2024Quote: ... and the number of cells that were alive or dead were quantified using Gen 5 software (Agilent Technologies).
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bioRxiv - Cell Biology 2019Quote: ... The anc-1 3’UTR RNAi construct was generated by PCR amplifying part of the 3’UTR-region of the anc-1 transcript using Paq5000™ DNA Polymerase (Agilent Technologies, 600682). The primer sequences are detailed in Table S2 ...
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bioRxiv - Plant Biology 2020Quote: ... The sample components were separated on a C18 olumn (Hypersil GOLD C18; 4.6 mm × 100 mm; 3 µm particle size; Agilent, Santa Cruz, CA, USA). The column was eluted at 0.3 mL/min with a gradient from 95% solvent A (0.1% formic acid in water ...
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bioRxiv - Developmental Biology 2022Quote: ... High quality total RNA (RNA integrity number = 10) was labelled with Cyanine 3 CTP using the Low Input Quick Amp Labelling Kit (Agilent Technologies-5190-2305), and purified using Qiagen’s RNeasy Mini Spin Columns ...
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bioRxiv - Biochemistry 2022Quote: ... All libraries at this step yielded >300,000 colonies implying a 100x coverage (assuming 1/3 of the variants were perfect based on our previous analysis of Agilent OLS based libraries). Each sublibrary was combined at an equimolar ratio to make a complete library with all intended mutations included.
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bioRxiv - Microbiology 2021Quote: ... The extracts were acidified to pH 2 and additionally pre-treated with solid-phase extractions using Agilent Bond Elut-PPL 3 mL columns and diluted to 50 ppm (Agilent Technologies, DE, USA) following standard lab protocol29 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Primary antibodies were diluted 1:4000 for anti-sGCα1 and 1:2000 for anti-sGCβ1 antibody in 3% dry milk in TBST and incubated with nitrocellulose membranes at 4°C over-night following challenge of membranes with secondary goat anti-rabbit antibody (1:2000 in 3% milk in TBST) conjugated to horseradish peroxidase (Dako A/S, Denmark). Immuno-complexes were visualized using an enhanced chemiluminescence kit (Amersham Pharmacia Biotech ...
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bioRxiv - Biochemistry 2022Quote: 50 μL of pure CrRPE1 concentrated at 9.5 mg mL−1 were injected on BioSEC-3 300 size-exclusion chromatography column (Agilent Technologies, Santa Clara, USA) equilibrated in buffer 20 mM Tris-HCl (pH 7.9 ...
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bioRxiv - Biophysics 2022Quote: ... The samples were then further diluted in ultrapure water to a final 3% nitric acid concentration and analyzed by triple quadrupole inductively coupled plasma-mass spectrometry (Agilent 8800 ICP-QQQ). Copper concentrations were determined by using standard curves ...
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bioRxiv - Synthetic Biology 2022Quote: ... 5’- ACC TCC ACC ACC AGA ACC ACC TCC ACC GCT CTG AAA GTA CAG ATC CTC GGT ACC TTT ATA TAA TTC GTC CAT-3’) using the Agilent QuikChange SDM kit following the manufacturer’s instructions (Agilent, Singapore, Singapore; Cat: STR_210519). This was followed by amplifying the backbone of the plasmid using primer set Ag43BBGib_F ...
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bioRxiv - Synthetic Biology 2022Quote: ... 5’- CTT TTT CAA ATT GGG GGT GTG ACC ATG CAG CGC TAG CCG CAG C −3’) using the GeneMorph II epPCR kit (Agilent, Singapore, Cat: STR_200550). The epPCR profile is as follows ...
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Age, sex, and cell type-resolved hypothalamic gene expression across the pubertal transition in micebioRxiv - Genomics 2023Quote: RNA-seq libraries were prepared using an automated - QuantSeq 3’mRNA-seq (Lexogen GmbH, Vienna) and Agilent NGS Workstation (Agilent Technologies, Santa Clara) at The Centre for Applied Genomics (TCAG ...
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bioRxiv - Genomics 2020Quote: ... The genomic DNA from the positive control was resubmitted for WES analysis using the SureSelect Human All Exon V6 kit from Agilent (performed by BGI Genomics Co, China) to compare both WES approaches ...
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bioRxiv - Molecular Biology 2019Quote: ... The mixture was pre-incubated at 37°C for 5 min and AffinityScript Multiple Temperature Reverse Transcriptase (Agilent Technologies) was added to each toeprinting reaction at a final content of 10.2% (v/v ...
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bioRxiv - Evolutionary Biology 2021Quote: ... An Agilent J&W HP-5 column (30 m × 0.25 mm i.d., 0.25 μm film thickness; Agilent Technologies, USA) was used in the GC ...
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bioRxiv - Immunology 2022Quote: ... the membrane was incubated in secondary antibody solutions (TBS containing 0.1 % Tween-20 and 5 % BSA, HRP-conjugated secondary antibodies (1:5000, Dako)) at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... proteins were desalted online by reversed-phase chromatography on a Poroshell 300SB C3 column (1.0 × 75 mm, 5 μm, Agilent Technologies ...
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bioRxiv - Microbiology 2020Quote: ... Separation occurred with a HP-5 ms capillary column coated with 5% phenyl-95% methylpolysiloxane (30 m×250 µm i.d., 0.25 µm film thickness, Agilent Technologies).
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bioRxiv - Molecular Biology 2019Quote: EMSAs were performed as described and cross-linked for 5 min (∼720 mJ) in a UV Stratalinker 1800 (Stratagene). Samples were taken up in 2x SDS loading buffer and boiled for 5 min before being loaded on a 7.5 % denaturing polyacrylamide gel and run for 1-2 hours in 10 % SDS-running buffer at 130 V ...
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bioRxiv - Microbiology 2019Quote: ... equipped with an HP-5 capillary column (60 m × 0.25 mm × 0.25 μm, Agilent Technologies, Inc., Santa Clara, CA). Pure helium (99.9999 % ...
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bioRxiv - Plant Biology 2019Quote: ... An HP-5MS capillary column (5%phenyl-methyl-siloxane, 30-m, 250-mm, and 0.25-mm film thickness; Agilent) was used with helium carrier gas at 2 mL/min ...
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bioRxiv - Synthetic Biology 2020Quote: ... Ten microliters of each diluted sample were injected and sample separation was achieved using a Zorbax Eclipse Plus C18 guard column (4.6 cm×12.5 cm, 5 μm packing, Agilent Technologies) connected to a Zorbax Eclipse Plus C18 column (4.6 mm × 100 mm ...
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bioRxiv - Biophysics 2020Quote: ... They were then stained for 5 min with 4,6-diamidino-2-phenylindole (DAPI) and mounted using fluorescence mounting medium (DAKO, Agilent). Pictures were taken with an UltraVIEW ERS spinning disk confocal microscope (Zeiss 63X/1.4 ...
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bioRxiv - Cancer Biology 2021Quote: ... the TMT-labelled peptides were fractionated using high pH reverse phase chromatography on a C18 column (150 × 2.1 mm i.d. - Kinetex EVO (5 μm, 100 Å)) using an HPLC system (Agilent, LC 1260 Infinity II ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 μg of pooled total RNA were tested for RNA quality on an ABI2000 Bioanalyzer (Agilent; Santa Clara, CA), labeled ...
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bioRxiv - Biochemistry 2020Quote: Concentrated protein samples were diluted to 0.02 mg/mL in 0.1% formic acid and 50 μL was injected on to a 2.1 mm x 12.5 mm Zorbax 5 μm 300SB-C3 guard column (Agilent Technologies) housed in a column oven set at 40°C ...
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bioRxiv - Microbiology 2021Quote: ... with a 30 x 0.25 mm (0.25 µm thickness) DB-5 MS capillary column (Agilent J & W capillary GC) and coupled to a Polaris Q benchtop Ion Trap Mass Spectrometer ...
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bioRxiv - Biophysics 2020Quote: ... in 20 min at a flow rate of 0.4 mL min-1 using a Zorbax Narrow Bore (2.1×50 mm, 5 μm) C18 column (Agilent Technologies).
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bioRxiv - Cancer Biology 2022Quote: ... HPTS and RuBPY fluorescence were monitored for 17 h using a Cytation 5 device (BioTek, Agilent, Winooski, VT, USA). Excitation was provided by a monochromator ...
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bioRxiv - Cancer Biology 2022Quote: ... and read by brightfield and fluorescence imaging on a BioTek Cytation 5 Cell Imaging Multi-Mode Reader (Agilent Technologies) every six hours for a total of six days ...
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bioRxiv - Biochemistry 2022Quote: ... The gas chromatograph was equipped with a DB-5 ms column (30 m × 0.25 mm, film thickness 0.25 μm; including 10 m DuraGuard column, Agilent Technologies) and a GC inlet liner (ultra inert ...
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bioRxiv - Synthetic Biology 2022Quote: ... fitted with a guard column (Zorbax Eclipse XDB-C18, 2.1 x 5 mm 1.8 μm, Agilent part # 821725-903) using a 1290 Infinity II UHPLC (G7120AR ...
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bioRxiv - Molecular Biology 2022Quote: ... pH 9.0 for 30 minutes followed by a 5-minute incubation in DAKO Peroxidase Block (DAKO, Catalog No. S2001) to block endogenous peroxidase activity ...
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bioRxiv - Plant Biology 2021Quote: Quantitative analysis was performed using a HP-5MS capillary column (5% phenyl-methyl-siloxane, 30 m x 250 mm, 0.25 mm film thickness; Agilent) with helium carrier gas at 1.5 mL/min ...
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bioRxiv - Microbiology 2021Quote: ... samples (5 uL) were injected onto a ZORBAX RRHD Extend-C18 column (2.1 x 150 mm, 1.8 µm; Agilent Technologies) with a ZORBAX SB-C8 (2.1 mm × 30 mm ...
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bioRxiv - Neuroscience 2020Quote: ... and loaded onto a ZORBAX Extend 300 C18 columns (4.6 mm x 250 mm with 5 µm beads) from Agilent. An Agilent 1100 HPLC system was used to carry out the fractionation ...
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bioRxiv - Microbiology 2022Quote: ... containing peptaibols was carried out by semi-preparative HPLC on an SSI system (Teledyne SSI Lab Alliance Series III pump system and Series 1500 Photodiode Array Detector) with an Eclipse XDB-C18 column (9.4 × 250 mm, 5 μm, Agilent). Acetonitrile (A ...
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bioRxiv - Microbiology 2022Quote: ... After centrifugation plate was incubated for 5 minutes before being loaded into a Seahorse XF96 Extracellular Flux Analyzer (Agilent). Basal respiration was measured over six hours with 36 ten-minute protocol cycles including a ...
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bioRxiv - Biochemistry 2022Quote: ... Sample from each PNT3 variant at 5 mg mL-1 in buffer B containing 6M GDN was injected onto an AdvanceBio SEC 2.7 µm (Agilent) SEC column ...
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bioRxiv - Cancer Biology 2020Quote: ... and incubated with 1 × blocking buffer (5% goat serum [#X0907, Dako], 2.5% BSA, 0.1% Triton X-100 in PBS) (38) ...
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bioRxiv - Microbiology 2019Quote: ... A Zorbax SB-C8 reversed-phase column (5 μm, 2.1 × 50 mm) was obtained from Agilent (Palo Alto, CA). The LC eluent was introduced into the ESI source of the mass spectrometer ...
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bioRxiv - Plant Biology 2019Quote: ... 25 μg of peptides were loaded unto 20 cm capillary columns packed with 5 μM Zorbax SB-C18 (Agilent), which was connected using a zero dead volume 1 μm filter (Upchurch ...