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Citations for Agilent :
1651 - 1700 of 2440 citations for Recombinant Human Epithelial Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... cells were mounted on glass slides using Dako mounting medium (Agilent; Santa Clara, CA). Antibody concentrations are provided in Table S3 ...
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bioRxiv - Cancer Biology 2024Quote: Mahlavu and Huh7 cells were plated into E-96 plates (Agilent, cat. no:5232368001), 1500-2500 cells/wells ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were washed and incubated with assay media (Agilent Technologies, Santa Clara, CA) supplemented with 1 mM glucose (Agilent Technologies ...
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bioRxiv - Biophysics 2021Quote: ... cells were resuspended in 200 μL PBS containing 1% BSA for detection by NovoCyte (Agilent) utilizing laser excitation and emission wavelengths of 488 nm and 530 nm ...
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bioRxiv - Cancer Biology 2021Quote: ... cells that were kept in GM were washed and incubated in XF Base Medium (Agilent) containing 1 mM sodium pyruvate ...
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bioRxiv - Neuroscience 2022Quote: ... the plasmid was amplified using chemically competent cells deficient for recombinase activity (Agilent Technologies #200152), purified (Macherey-Nagel #740416.10) ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed and analyzed by flow cytometry (NovoCyte Flow Cytometer Systems, Agilent, CA, USA). The tetramer positive staining wells were pooled and CD8/Tetramer double positive population were sorted using flow cytometric sorting (ARIA II sorter ...
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bioRxiv - Developmental Biology 2019Quote: ... cell culture medium was replaced with the appropriate pre-warmed Seahorse XF Base Medium (Agilent Biotechnology ...
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bioRxiv - Immunology 2019Quote: Cell culture supernatants were harvested and concentrated with StrataClean resin beads (Agilent Technologies, CA, USA), as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: The pHMT-KTN1 plasmid was transformed into BL21-CodonPlus (DE3)-RIPL competent cells (Agilent Technologies). Protein was affinity-purified using Ni-NTA agarose (Qiagen #301210) ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed and analyzed by flow cytometry (NovoCyte Flow Cytometer Systems, Agilent, CA, USA). The tetramer positive staining wells were pooled and CD8/Tetramer double positive population were sorted using flow cytometric sorting (ARIA II sorter ...
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bioRxiv - Biochemistry 2021Quote: ... Expression vectors containing gene of interest were transformed into BL21-Gold (DE3) competent cells (Agilent) and the cells were cultured to a density of ~1 OD600/ml at 37 °C ...
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bioRxiv - Cell Biology 2020Quote: ... Barcoded single cell capture plates were prepared with a Bravo automated liquid handling platform (Agilent) as described previously28 ...
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bioRxiv - Cancer Biology 2020Quote: ... All cell lines were tested for mycoplasma using the PCR based mycoplasma detection kit (Agilent).
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bioRxiv - Cell Biology 2021Quote: ... OCR and ECAR were measured using Seahorse XF Cell Mito Stress Test Kit (Agilent Technologies). 1 × 104 cells/well were plated onto 7 μg/mL Poly-D-Lysine coated (Ruhanen et al ...
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bioRxiv - Neuroscience 2020Quote: ... Washing procedure was repeated and cells were coversliped using an aqueous fluorescent mounting medium (Dako). The specimens were analysed under a confocal microscope Leica TCS SP8 (Leica Microsystems ...
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bioRxiv - Neuroscience 2020Quote: Mitochondrial function was assessed using a Seahorse XF24 cell MitoStress Test (Agilent Technologies, California, USA) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: ... mitochondrial stress test was performed using a Seahorse XF Cell Mito Stress Test kit (Agilent) according to the manufacture manual ...
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bioRxiv - Microbiology 2020Quote: ... Pt cones and an Octopole Reaction System (ORS) collision cell (Agilent Technologies, Santa Clara, CA). The data was collected using a 3-point peak pattern ...
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bioRxiv - Cell Biology 2020Quote: ... Barcoded single cell capture plates were prepared with a Bravo automated liquid handling platform (Agilent) as described previously16 ...
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bioRxiv - Biophysics 2020Quote: The hSirt3102-399 was expressed in E.coli Arctic Express (DE3) cells (Agilent Technologies, Wilmington, DE) as per manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2022Quote: ... HEK-T-cells were transfected with the help of the AAV Helper-Free System (Agilent) and after three days harvested and concentrated the Virus with the AAVanced™ Concentration Reagent (System Biosciences).
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bioRxiv - Neuroscience 2022Quote: ... pAAV plasmids were co-transfected with a pDP6 helper plasmid into HEK293-AAV cells (Agilent). Cells were lysed 72 h after transfection and viral particles were purified using Iodixanol gradient followed by separation ion-exchange chromatography (GE Healthcare) ...
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bioRxiv - Molecular Biology 2022Quote: ... The single cell libraries were assessed for quality (TapeStation 4200, Agilent, Santa Clara, CA, USA) and then run by using paired-end 50 bp sequencing on the Illumina HiSeq 4000 platform (Illumina ...
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bioRxiv - Immunology 2022Quote: ... The cells were fixed with 100 ml chilled methanol and incubated with Peroxidase Block (Dako) followed by mouse anti-CMV IE-1/IE2 mAb (Chemicon ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were then antibody stained with guinea-pig anti insulin (1:500, Dako, Carpinteria, CA), Alexa Fluor 647 anti guinea-pig (1:1000 Thermo Fisher ...
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bioRxiv - Biophysics 2022Quote: ... at a density of 16.000 cells per well using the liquid handler BRAVO from Agilent equipped with a 96-LT (Large Tips ...
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bioRxiv - Biochemistry 2022Quote: ... Peptides were expressed in BL21-Codon Plus (DE3) RIPL cells (Agilent, Santa Clara, CA, USA) in ZYP-5052 auto-induction media(Studier ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell lines were tested for Mycoplasma contamination using MycoSensor PCR Assay Kit (Agilent, Didcot, UK).
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bioRxiv - Biochemistry 2021Quote: ... All AtAtm3 constructs were overexpressed in Escherichia coli BL21-gold (DE3) cells (Agilent Technologies, CA) using ZYM-5052 autoinduction media as described previously (Fan et al. ...
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bioRxiv - Molecular Biology 2019Quote: ... The Seahorse XF Cell Mito Stress test was performed on a Seahorse XFe96 Analyzer (Agilent) in the XF Base Medium (Agilent ...
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bioRxiv - Physiology 2022Quote: ... Tubulointerstitial cell proliferation was detected by a monoclonal mouse anti-PCNA antibody (Dako, Glostrup, Denmark), while collagen I and fibronectin positivity were detected with polyclonal anti-collagen I (Rockland Immunochemicals ...
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bioRxiv - Plant Biology 2019Quote: ... the pET21 plasmid containing PL1 was transformed into BL21 DE3 pLyS cells (Agilent, Edinburgh, UK). PL1 expression was induced at mid-log phase by supplementing the media with 0.3 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) ...
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bioRxiv - Cell Biology 2019Quote: RNA was extracted from mouse embryonic stem cells using the Absolutely RNA microprep kit (Agilent). 1 µg of RNA was pre-incubated with 4.5 µg of (PR)20 or water for 10 min at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: RNA extraction from FACS-isolated cells was performed using the Absolutely RNA nanoprep kit (Stratagene) according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2021Quote: Assays were performed using a Seahorse XF96 Cell Mito Stress Test Kit (Agilent Biosciences, USA). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were resuspended in 200 μL PBS containing 1% BSA for detection by NovoCyte (Agilent) utilizing laser excitation and emission wavelengths of 488 nm and 530 nm ...
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Airway Gene-Expression Classifiers for Respiratory Syncytial Virus (RSV) Disease Severity in InfantsbioRxiv - Immunology 2020Quote: ... Recovered cells were lysed and homogenized using the AbsolutelyRNA Miniprep kit (Agilent, Santa Clara, CA) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... coli cells by the heat-shock approach as described in the supplier’s manual (Novagen/Agilent) and by antibiotic selection using Zeocin (Invitrogen/ThermoFisher) ...
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bioRxiv - Immunology 2020Quote: ... cells were barcoded using automated liquid handling robotic system (Agilent Technologies, Santa Clara, CA, USA)(38 ...
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bioRxiv - Immunology 2021Quote: ... 1.5 x 105 cells per well were seeded into seahorse culture plate (Agilent, 102416-100) precoated with Cell-Tak (Corning ...
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bioRxiv - Genetics 2021Quote: ... All compounds were from a Seahorse XF Cell Mito Stress Test Kit (Agilent, 103015-100). Four OCR measurements were recorded for baseline and following each injection ...
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bioRxiv - Biochemistry 2020Quote: ... The hSirt3,102-399 was expressed in E.coli Arctic Express (DE3) cells (Agilent Technologies, Wilmington, DE) as per manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2021Quote: ... cell culture media was washed and replaced with fresh assay media (XF basic media; Agilent), supplemented with 10 mM Glucose ...
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bioRxiv - Genetics 2019Quote: ... The PCR product was ligated with T4 ligase prior to transformation into competent cells (Stratagene). The plasmid was sequenced to validate the removal of the putative EGR1 binding site ...
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bioRxiv - Neuroscience 2021Quote: ... AAV8- GFAP-OptoA1-eYFP was produced by standard triple transfection of AAV 293 cells (Agilent). At 72 h post-transfection ...
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bioRxiv - Physiology 2021Quote: ... The cells were washed twice with 0.5 ml Seahorse XF DMEM medium pH 7.4 (Agilent) and then incubated in a CO2-free incubator at 37 °C (or 24°C for S2 cells ...
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bioRxiv - Immunology 2020Quote: ... BMDCs (80,000 cells/well) were analyzed with an XF-96 Extracellular Flux Analyzer (Seahorse Bioscience). 1-2 x 105 cells/well were used in 96-well tissue-culture treated flat bottom plates for the metabolic measurements ...
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bioRxiv - Immunology 2021Quote: ... monocytes were seeded as a monolayer onto XFe24 cell culture plates (Seahorse Bioscience, MA, USA). The culture media was replaced with XF assay media supplemented with l-glutamine (250 μg/ml ...
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bioRxiv - Immunology 2021Quote: ... the cell culture medium was replaced with XF RPMI medium pH 7.4 (Agilent, 103576-100) supplemented with 2 mM glutamine prior to analysis ...