Labshake search
Citations for Agilent :
1551 - 1600 of 2610 citations for Recombinant Human Epithelial Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2024Quote: Ubiquilins were expressed in BL21 DE3 cells with the pRIL plasmid (Agilent). Cells were grown in terrific broth at 37°C until OD600 = 0.6-0.8 ...
-
bioRxiv - Biochemistry 2024Quote: The cells were analyzed with a PicoLE Molecular Imaging system from Agilent Technologies (Keysight Technologies ...
-
bioRxiv - Microbiology 2024Quote: ... Relative fluorescence was measured by BioTek Cytation 5 Cell Imaging Multimode Reader (Agilent Technologies, Santa Clara, CA, USA) with an excitation wavelength of 530 nm and emission wavelength of 560-640 nm ...
-
bioRxiv - Microbiology 2024Quote: ... cell density was measured at 764 nm (Agilent Cary 60 UV-Vis).
-
bioRxiv - Cell Biology 2024Quote: ... The ligation mixture was transformed into XL10-Gold competent cells (Agilent, 200315). Resulting plasmids were verified by sequencing.
-
bioRxiv - Developmental Biology 2024Quote: ... PSM cells were re-seeded on a fibronectin-coated Seahorse plate (Agilent) at a density of 9.1 × 105 cells/cm2 (mouse assay ...
-
bioRxiv - Cell Biology 2020Quote: ... Inoculated a colony of BL21-CodonPlus(DE3)-RIL competent cells (Agilent Technologies 230245) transformed with the plasmid DNA in 10 ml of LB media supplemented with kanamycin and grew overnight at 37°C ...
-
bioRxiv - Cell Biology 2019Quote: ... cells were pre-incubated with blocking buffer (Protein Block Serum Free solution, DAKO) for 30 min at room temperature ...
-
bioRxiv - Immunology 2021Quote: ... Cells were adhered to each well of the Seahorse plate (Seahorse/Agilent, USA) using CellTak (Corning ...
-
bioRxiv - Molecular Biology 2020Quote: ... cells were washed with Seahorse XF DMEM Medium pH 7.4 (Agilent, 103575-100) supplemented with 10mM glucose ...
-
bioRxiv - Molecular Biology 2021Quote: ... coverslips containing cells were mounted with fluorescent mounting media (DAKO, Carpinteria, CA, USA) on coverslips ...
-
bioRxiv - Molecular Biology 2019Quote: ... The sequence-verified construct was transformed into JM101 cells (Agilent Technologies cat. #200234) for expression ...
-
Weak Membrane Interactions Allow Rheb to Activate mTORC1 Signaling Without Major Lysosome EnrichmentbioRxiv - Cell Biology 2019Quote: ... and transformed into XL-1 Blue Supercompetent Cells (Agilent Technologies, Santa Clara, CA). Plasmid DNA was isolated from multiple colonies and sequenced.
-
bioRxiv - Microbiology 2019Quote: ... Cells were irradiated with 900 J/cm2 254 nm light (Stratagene Stratalinker 2400). Control cultures were resuspended in MgSO4 as above ...
-
bioRxiv - Molecular Biology 2019Quote: ... Bacteria for histone expression were BL21(DE3)pLysS and BL21(DE3) cells (Agilent). Transformed bacteria were grown on 2xTY media (1.6% Bacto Tryptone ...
-
bioRxiv - Cell Biology 2019Quote: ... cells were washed with 100 μL XF Base medium (Seahorse Bioscience 102353-100) which has been adjusted to pH 7.4 at 37 °C with 0.1M NaOH ...
-
bioRxiv - Immunology 2020Quote: The Cell Mito Stress Test was performed on a Seahorse XFe96 Analyser (Agilent) to assess mitochondrial function and OXPHOS ...
-
bioRxiv - Cancer Biology 2020Quote: ... cells were washed with complete Seahorse XF media (Cat# 103334-100, Agilent Technologies) supplemented to final concentrations of 25 mM glucose ...
-
bioRxiv - Molecular Biology 2021Quote: ... Individual cells were collected in 384-well plates with ERCC spike-ins (Agilent), reverse transcription primers and dNTPs (both Promega) ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were washed and mounted with Dako Faramount Aqueous Mounting Medium (S3025, Agilent).
-
bioRxiv - Cell Biology 2020Quote: ... cells were mounted on microscope slides using Dako Fluorescent Mounting Medium (Dako, S3023).
-
bioRxiv - Biophysics 2020Quote: Plasmids were transformed into BL21-CodonPlus(DE3)-RIPL competent cells (Agilent Technologies #230280). A single colony was inoculated in 1 mL of terrific broth (TB ...
-
bioRxiv - Cancer Biology 2021Quote: Metabolism profiling was carried out by Agilent Seahorse XFp Cell Energy Phenotype test kit on a Seahorse XFp analyser (Agilent). Protocols were carried out according to manufacturer guidelines and data was analysed using Agilent Seahorse Wave desktop V2.5 ...
-
bioRxiv - Cancer Biology 2021Quote: ... prior to OCR measurements using the XF Cell Mito Stress Kit (Seahorse Bioscience). Concentrations of oligomycin and FCCP were adjusted for each primary cell type ...
-
bioRxiv - Biophysics 2022Quote: Escherichia coli BL21-CodonPlus(DE3)-RIPL cells (Agilent Technologies, Santa Clara, CA, USA) were transformed with either pET15-MinD ...
-
bioRxiv - Immunology 2022Quote: ... All cells were measured using the Seahorse XF Glycolysis Stress Test Kit (Agilent) and Seahorse XF Cell Mito Stress Test Kit (Agilent ...
-
bioRxiv - Biophysics 2022Quote: ... His6-SUMO-TTL was expressed in Escherichia coli (BL21-DE3 competent cells, Agilent) overnight at 18 °C in LB broth (induced by 0.5 mM IPTG) ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cell cycle distribution was analyzed with the Cytomation Summit software (Dako version 4.3). To assess cell death ...
-
bioRxiv - Neuroscience 2021Quote: ... HeLa cells were mounted using Dako Fluorescence Mounting Medium (S3023, Agilent Technology, USA).
-
bioRxiv - Cell Biology 2021Quote: Real-time cell metabolic analysis was performed on a Seahorse XFe96 Analyzer (Agilent) using a XF Cell Mito Stress Test kit (Agilent 103015-100 ...
-
bioRxiv - Biochemistry 2021Quote: pMtac expression plasmids were transformed into BL21-Codon Plus RIPL competent cells (Agilent). Expression of His6-tagged proteins were induced at 16°C overnight with 0.25 mM IPTG ...
-
bioRxiv - Microbiology 2022Quote: ... Cells were incubated with anti-VP1 antibodies (Dako, anti-enterovirus clone5-D8/1) diluted 1:500 in blocking solution for detection of viral protein VP1 or anti dsRNA antibody (SCICONS ...
-
bioRxiv - Neuroscience 2022Quote: ... where we used the XL 10-Gold Ultracompetent Cells (Agilent, cat. no. 200315) provided with the mutagenesis kit.
-
bioRxiv - Neuroscience 2022Quote: ... the plasmid was transformed into BL21-CodonPlus (DE3)-RIPL competent cells (Agilent, #230280). A single colony was inoculated in 1-mL TB medium (Laboratory ...
-
bioRxiv - Biochemistry 2022Quote: ... Each construct was transformed into Escherichia coli BL21(DE3) Codon Plus Cells (Agilent). Select colonies were inoculated in 50 ml of Luria Broth (LB ...
-
bioRxiv - Biochemistry 2019Quote: ... All constructs were overexpressed in Escherichia coli BL21-gold (DE3) cells (Agilent Technologies) using ZYM-5052 autoinduction media (Studier 2005) ...
-
bioRxiv - Developmental Biology 2019Quote: Total RNA was isolated from cells using the Absolutely RNA Miniprep Kit (Agilent). cDNA was generated using Maloney murine leukaemia virus reverse transcriptase (MMLV-RT ...
-
bioRxiv - Cancer Biology 2020Quote: ... Cells were tested negative for mycoplasma using the Mycosensor PCR assay kit (Agilent) or the LookOut Mycoplasma PCR detection kit (Sigma-Aldrich).
-
bioRxiv - Biophysics 2020Quote: ... were expressed from a pet29b vector in BL21 Gold (DE3) cells (Agilent Technologies). Cultures were grown to an OD600 of 0.6 then induced with 0.4 mM IPTG and left to express at 18 °C overnight ...
-
bioRxiv - Cancer Biology 2020Quote: ... Cell culture media was replaced with non-buffered DMEM base media (Seahorse bioscience) containing 25mM glucose ...
-
bioRxiv - Physiology 2020Quote: ... and rotenone/antimycin A (Port C) (XF Cell Mito Stress Test Kit, Agilent) to achieve final concentrations of 1 μM ...
-
bioRxiv - Physiology 2020Quote: ... the cells were washed and incubated with unbuffered XF assay medium (Agilent;103575) contained 5 mM glucose ...
-
bioRxiv - Neuroscience 2020Quote: ... Inhibitors targeting the different mitochondrial complexes (Cell Mito Stress Test kit, Seahorse Bioscience) were added automatically and sequentially to the cells during the experiment to measure the basal respiration ...
-
bioRxiv - Plant Biology 2019Quote: ... and transformed into XL1-BLUE Escherichia coli competent cells (Stratagene, Santa Clara, USA). Three hundred and thirty-six clones (48 from each library ...
-
bioRxiv - Immunology 2020Quote: SARS-CoV-2 pseudovirus was produced using HEK293T cells transfected with GeneJammer (Agilent) using IgE-SARS-CoV-2 spike plasmid (Genscript ...
-
bioRxiv - Physiology 2021Quote: ... Cell metabolic rates were measured using XF96 Extracellular Flux Analyzer (Seahorse Bioscience, Agilent). Oligomycin (2µM) ...
-
bioRxiv - Physiology 2021Quote: ... Cell metabolic rates were measured using XF96 Extracellular Flux Analyzer (Seahorse Bioscience, Agilent). Oligomycin (2µM) ...
-
bioRxiv - Biochemistry 2021Quote: ... All DNA variants were amplified with XL-10 Gold Ultracompetent cells (Agilent Technologies).
-
bioRxiv - Cell Biology 2020Quote: Citrate Synthase Normalization Assay was performed on Seahorse XF96 Cell Culture Microplate (Agilent) right after seahorse assay or on plates frozen at −80°C with leftover medium aspirated ...
-
bioRxiv - Physiology 2021Quote: Respiration in cells were measured using Seahorse XF96 Analyzers (Agilent, Santa Clara, CA) according to Wong et al ...