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Citations for Agilent :
1601 - 1650 of 2440 citations for Recombinant Human Epithelial Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... All three proteins were expressed in BL21-Gold (DE3) competent cells (Agilent Technologies; 230132). The cells were cultured in 4 L of Luria Bertani broth (LB ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were then mounted on glass slides in fluorescence mounting medium (DAKO, Carpinteria, CA).
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bioRxiv - Microbiology 2019Quote: ... The clone was verified by sequencing and transformed into Arctic Express competent cells (Agilent) for protein expression ...
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bioRxiv - Developmental Biology 2019Quote: ... as it has been previously established to be appropriate for most cell types (Agilent Seahorse XF Cell Mito Stress Test Kit User Guide ...
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bioRxiv - Microbiology 2019Quote: ... The cells were stained with HAstV mouse monoclonal antibody 8E7 (2 μg/ml DakoCytomation) for 1 hour at room temperature followed by anti-mouse IgG labeled with Alexa Fluor 488 (anti-mouse IgG-Alexa Fluor 488 ...
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bioRxiv - Molecular Biology 2019Quote: ... at 1×104 cells per well and equilibrated with Seahorse XF Base Medium (Agilent) supplemented with 1 mM pyruvate ...
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bioRxiv - Microbiology 2020Quote: ... High concentration plasmid solutions were produced using XL1-Blue Competent Cells (200249, Agilent technology) and plasmid extraction was performed using Qiagen Plasmid Maxikit (12163 ...
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bioRxiv - Immunology 2021Quote: ... 1×106 small intestinal cells were cultured in glucose-free DMEM medium (Agilent, USA) supplemented with 2mM L-glutamine and 100μM 2-NBDG (Thermo Fischer ...
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bioRxiv - Immunology 2021Quote: ... 1×106 small intestinal cells were cultured in glucose-free DMEM medium (Agilent, USA) supplemented with 2mM L-glutamine and LipidTOX™ (Thermo Fischer ...
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bioRxiv - Immunology 2020Quote: ... We used the Seahorse XF Cell Mito Stress Test Kit (103015-100, Seahorse Bioscience) for the OCR assay ...
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bioRxiv - Immunology 2020Quote: ... T cells were measured at 37°C using an Xfe96 extracellular analyzer (Seahorse Bioscience). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: Real-time cell proliferation was monitored using an xCELLigence RTCA DP instrument (Agilent Technologies) with RTCA 16-well E-plate and placed inside an incubator at 37 °C and with 5% CO2 ...
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bioRxiv - Cancer Biology 2022Quote: ... and programmed cell death ligand 1 (PD-L1; clone 22C3, pharmDx kit, Agilent, USA) were used ...
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bioRxiv - Genomics 2022Quote: ... cells were analyzed by NovoCyte Quanteon Flow Cytometer Systems (Agilent, Santa Clara, CA, USA).
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bioRxiv - Physiology 2022Quote: Isolated CMs were seeded on matrix gel–coated cell culture microplates (Agilent Seahorse XF24) at the cell intensity of 20000 cells/well ...
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bioRxiv - Neuroscience 2022Quote: ... cells were washed once with PBS before being submerged in fluorescence mounting medium (Dako), and stored at 4°C until imaging.
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bioRxiv - Microbiology 2022Quote: The xCELLigence Real-Time Cell Analyzer (RTCA) DP instrument (Agilent, Santa Clara, CA, USA) was used to measure changes in cellular impedance following addition of cells on top of the monolayer ...
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bioRxiv - Cancer Biology 2023Quote: ... using the Seahorse XF Cell Mito Stress Test Kit (Agilent Technologies, Cat. 103015-100) and following the instructions of the manufacturer ...
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bioRxiv - Molecular Biology 2023Quote: ... coli cells after was taken for RFP analysis using fluorescence spectrophotometer (Cary Eclipse, Agilent) with excitation wavelength of 550 nm ...
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bioRxiv - Cell Biology 2023Quote: Chemotaxis assays were performed using Real-Time Cell Analysis (RTCA) CIM-16 plates (Agilent). The lower chamber contained conditioned medium as the chemoattractant or DMEM supplemented with 0.5% (v/v ...
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bioRxiv - Molecular Biology 2023Quote: Resuspended cells were plated in the XF96 tissue culture microplate (Agilent Technologies, Seahorse Bioscience) at a concentration of 3 x 105 cells per well in 180 μL of growth media (DMEM with 5.56 mM glucose ...
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bioRxiv - Cell Biology 2023Quote: ... and 2 µM Antimycin A + Rotenone (Seahorse XF Cell Mito Stress Test Kit, Agilent) was added sequentially via injection ports ...
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bioRxiv - Biophysics 2023Quote: dCas9-Cys protein expression was induced in BL21-CodonPlus(DE3)-RIL cells (Agilent #230245) transformed with plasmid 10xHis-MBP-TEV-S ...
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bioRxiv - Cell Biology 2023Quote: Bacterial expression constructs were transformed to BL21-CodonPlus(DE3)-RIL competent cells (Agilent Technologies). Fresh colonies were cultured into LB with antibiotics overnight at 37 °C and cultured further after 50 times dilution at 37 °C until OD600 values reach to 0.5-0.8 ...
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bioRxiv - Physiology 2023Quote: Plasmid constructs used for transient transfection were amplified using XL10-gold ultracompetent cells (Agilent Technologies Inc. ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed 3 times with 100 µL of XF Base Media (Seahorse Bioscience) containing 2 mM L-Glutamine ...
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bioRxiv - Molecular Biology 2023Quote: Resuspended cells were plated in the XF96 tissue culture microplate (Agilent Technologies, Seahorse Bioscience) at a concentration of 3 x 105 cells per well in 180 μL of growth media (DMEM with 5.56 mM glucose ...
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bioRxiv - Biophysics 2023Quote: ... cerevisiae Cdc6 protein expression was induced in BL21-CodonPlus(DE3)-RIL cells (Agilent #230245) transformed with pGEX-6P-1 wt GST-cdc6 using 400 µM IPTG for 16 h at 16 °C ...
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bioRxiv - Physiology 2023Quote: ... cells were incubated in Seahorse XF assay DMEM medium (Agilent, 103575-100, pH 7.4) supplemented with 10 mM glucose ...
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bioRxiv - Immunology 2023Quote: ... in vitro-differentiated Th17 cells were seeded in XF Seahorse DMEM medium (Agilent Technologies) supplemented with 1mM sodium pyruvate (Gibco) ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were then incubated with primary antibody to GFAP (1:1000, #Z0334, DAKO), S100b (1:500 ...
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bioRxiv - Biophysics 2022Quote: ... The clarified whole-cell extract was subjected to fluorescent size exclusion chromatography (Agilent HPLC) using a Superdex S200 column (GE Healthcare ...
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bioRxiv - Cancer Biology 2022Quote: ECAR of CML cells was measured using the Seahorse XF96 flux analyzer (Seahorse Bioscience). The XF96 well plate was coated with 22.6 μg/ml Cell-Tak solution (catalogue number ...
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bioRxiv - Cancer Biology 2022Quote: ... K562 cells were suspended in XF Assay Medium (catalogue no. 100965–000; Seahorse Bioscience) supplemented with 2 mM glutamine and seeded at a concentration of 70,000-100,000 cells/well in the pre-coated XF96 well plate ...
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bioRxiv - Developmental Biology 2023Quote: Metabolic profiling of O9-1 cells was performed using a Seahorse XFe Analyzer (Agilent). O9-1 cells were seeded in preconditioned medium into Seahorse XFe 96 well plates at a density of 20,000 cells/well and incubated overnight ...
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bioRxiv - Physiology 2023Quote: ... AAV9-PDE2A was produced in AAV-293T cells (Stratagene #240073, San Diego, California, USA) with the three-plasmid method and the calcium-chloride transfection ...
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bioRxiv - Neuroscience 2023Quote: ... and the constructed AAV-vector plasmid were transfected into AAV-293 cells (#240073, Agilent) using Lipofectamine 2000 (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... which was followed by transformation in XL10-Gold bacterial cells (Agilent cat no. 200314). The cells were plated on ampicillin-positive LB-agar plates and incubated at 370C for 18 hours ...
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bioRxiv - Biochemistry 2023Quote: hSirt3102-399 was expressed in E.coli Arctic Express (DE3) cells (Agilent Technologies, Wilmington, DE), as previously described (30) ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells were allowed to equilibrate in XF Base media (glucose-free, Seahorse Bioscience) supplemented with 2mM glutamine at 37°C for 1h in a CO2-depleted incubator ...
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bioRxiv - Cancer Biology 2023Quote: ... plates were transferred to the Cytation 5 Cell Imaging Multi-Mode Reader (Biotek/Agilent) driven by Gen5 software (Biotek/Agilent) ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed once then fed Seahorse XF DMEM assay media (103575; Agilent Technologies) supplemented with 10 mM glucose (103577 ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were seeded in a 96 well Seahorse (Agilent, Technologies Inc., Santa Clara, USA) plate in 80µL ...
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bioRxiv - Immunology 2023Quote: ... cells were washed and kept on ice until acquisition on the MoFlo (Dako cytomation). RH5ΔNC-specific B cells were identified as live CD19+ IgG+ RH5ΔNC-APC+ RH5ΔNC-PE+ cells and single cell sorted into 96-well plates containing 10µL/well lysis buffer (10mM Tris [T3038 ...
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bioRxiv - Biochemistry 2023Quote: ... each plasmid was transformed into BL21-CodonPlus(DE3)-RIPL competent cells (Agilent Technologies, #230280). A single colony was picked and inoculated in 1 mL of terrific broth (TB ...
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bioRxiv - Genetics 2023Quote: ... All fibroblast cell lines were tested mycoplasma-free (MycoSensor QPCR assay, #302107, Agilent Technologies).
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bioRxiv - Neuroscience 2024Quote: ... were digested with DpnI and directly transformed into XL10-Gold ultracompetent cells (200315; Agilent). For PIEZO2 mutants ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were mounted on glass slides using Dako mounting medium (Agilent; Santa Clara, CA). Antibody concentrations are provided in Table S3 ...
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bioRxiv - Microbiology 2024Quote: ... and the percentage of GFP+ cells was quantified using a NovoCyte Quanteon cytometer (Agilent).
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bioRxiv - Cell Biology 2024Quote: ... the cells were washed and incubated with assay media (Agilent Technologies, Santa Clara, CA) supplemented with 1 mM glucose (Agilent Technologies ...