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Citations for Agilent :
1251 - 1300 of 1717 citations for Recombinant Human Ribulose 5 Phosphate 3 Epimerase His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... with a ZORBAX SB-C18 column (4.6 x 250 mm, 5 μm) (Agilent, Palo Alto, CA, USA) at 1 mL/min flow rate ...
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bioRxiv - Cancer Biology 2020Quote: ... baseline GFP intensity was collected using the BioTek Cytation 5 Cell Imaging Multi-Mode Reader (Agilent Technologies), and subsequently 48-hour conditioned media from HEK293 ...
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bioRxiv - Plant Biology 2021Quote: ... and 5 μl of this resuspension was loaded onto a C18 high capacity nano-LC chip (Agilent) in 98% Buffer A (0.1% (v/v ...
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bioRxiv - Cancer Biology 2021Quote: ... Analytes were separated on an Agilent PLRP-S column (5 μm; 2.1 × 50 mm, 1000Å Agilent Technologies) at 60 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were fractionated using Zorbax 300 Extend C18 column (4.6 × 250 mm, 300 Å, 5 μm, Agilent) on an Agilent 1100 series high-pressure liquid chromatography (HPLC ...
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bioRxiv - Microbiology 2020Quote: ... filter 5) and data were collected and processed using the Agilent ChemStation software (Agilent, Santa Clara, CA). For calibration ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were immersed in 5% normal non-immune goat serum (DakoX0907, Agilent Technologies Inc., CA, USA) for 15min and thereafter rinsed in 0.01M phosphate-buffered saline (PBS ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... whereby 5 μl of sample were injected in splitless mode onto a 90-m FastFAME Column (Agilent) using ultrapure helium (BOC ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... ON) mass spectrometer using a Eclipse Plus phenyl hexyl column (4.6 A 100mm, 5 µm column, Agilent) and 1290 Infinity II inline filter (0.3 µm) ...
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bioRxiv - Physiology 2023Quote: ... CP 3800 gas chromatographs equipped with a capillary column DB-5 (30 m x 0.25 mm I.D., 0.25 µm film thickness; Agilent) to separate extract components ...
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bioRxiv - Cancer Biology 2023Quote: ... Endogenous peroxidase activity was blocked for 5 min in using Dual Endogenous Enzyme Block (Cat. S2003, Dako). Primary Antibodies used included Ki67 (1:50 ...
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bioRxiv - Cancer Biology 2023Quote: ... The signal was developed with the Envision+ System/HRP Kit in 5–20 min (K4007, Agilent/Dako). Iba1+ cells were quantified based on the ImageJ plugin 46 ...
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bioRxiv - Cancer Biology 2023Quote: ... The signal was developed with the Envision+ System/HRP Kit in 5–20 min (K4007, Agilent/Dako). Iba1+ cells were quantified based on the ImageJ plugin 46 ...
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bioRxiv - Bioengineering 2023Quote: ... and impedance was measured at 10 kHz every 5 min using the xCELLigence RTCA SP device (Agilent) as described above ...
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative PCR (5 ng cDNA/well) was performed by using QuantiNova kit on Mx3500P PCR machine (Stratagene). Gene-specific primers were used for end-point PCR (HotStarTaq Plus DNA Polymerase ...
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bioRxiv - Microbiology 2024Quote: ... Supernatant (5 µl) from the centrifuged samples was injected into the instrument (Agilent 6490 triple quadrupole system). To make a calibration curve ...
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bioRxiv - Microbiology 2024Quote: ... Chromatographic separation of 5 µL sample was achieved on a 1290 Infinity II HPLC (Agilent Technologies, Germany) equipped with a Poroshell EC-C18 column (Agilent Technologies ...
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bioRxiv - Molecular Biology 2019Quote: ... or humanized Renilla luciferase (5’-CCC CGA GCA ACG CAA AC-3’ and 5’-GCA CGT TCA TTT GCT TGC A-3’) and in 1x Brilliant III Ultra-Fast SYBR® Green QPCR Master Mix (Agilent Technologies). The reaction and fluorescence readout were performed in Rotor-Gene 6200 (Corbett Life Science ...
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bioRxiv - Bioengineering 2019Quote: ... Removal of the native splice acceptor in the 3’ homologous arm of HDR templates was achieved via site-directed mutagenesis (Agilent Technologies, 200523).
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bioRxiv - Cell Biology 2020Quote: ... Membranes were washed in TBST (3 × 15 mins at RT) and then incubated with corresponding peroxidase-conjugated secondary antibodies (Dako, P0447, P0448) for 1h at RT ...
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bioRxiv - Cancer Biology 2020Quote: ... was performed on 3-μm thick tissue sections using split signal FISH DNA probes for BCL2/18q21 (probe Y5407; DAKO A/S), BCL6/3q27 (probe Y5408 ...
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bioRxiv - Bioengineering 2019Quote: ... Then endogenous peroxidase block was performed by using 3% H2O2 in water to cancel the interference of peroxidase in the final step.[33] Protein block buffer (Dako Protein Block) was used to treat the tissue slides to mask the non-specific binding sites ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were finally washed for 3 × 10min with PBS before mounting them on glass coverslips with Dako fluorescence conserving media (Dako, Hamburg, Germany).
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bioRxiv - Cell Biology 2021Quote: ... followed by incubation with 3% hydrogen peroxide to quench endogenous peroxidase activity and 15 minutes in serum-free protein block (DAKO, Glostrup, Denmark). Sections were then subjected to appropriate antigen retrieval methods (if needed ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were incubated overnight at 4° C with primary antibodies (mouse-anti-Ataxin-3, 1:2500, clone 1H9, MAB5360, MerckMillipore, Darmstadt, Germany; rabbit-anti-Ubiquitin, 1:500, Z0458, Dako, Jena, Germany). After washing the membranes were incubated with secondary antibodies (peroxidase affinePure donkey anti-mouse IgG H+L ...
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bioRxiv - Cancer Biology 2020Quote: To construct RNA-seq libraries from C.elegans samples, we used an automated QuantSeq 3’mRNA-seq (Lexogen GmbH, Vienna) using the Agilent NGS Workstation (Agilent Technologies, Santa Clara) as per manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... All immunostains were performed in Ventana Discovery (Ki-67 and cleaved caspase 3) or Dako autostainers using 3,3’ diaminobenzidine (DAB) chromogen (Dako-Agilent Technologies, Denmark). All slides were scanned using digital slide scanner NanoZoomer-XR C12000 (Hamamatsu ...
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bioRxiv - Cell Biology 2020Quote: ... embryos were washed 3 times in PBV and transferred onto a glass microscope slide with DAKO mounting medium (Dako Inc., Carpinteria, California) and enclosed with a coverslip using a spacer (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA libraries were assessed for quality and quantification according to the Chromium Single Cell 3’ Reagent kit v3 user guide using the High Sensitivity D5000 ScreenTape (Agilent 5067-5592) and 2200 TapeStation Controller software (Agilent ...
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bioRxiv - Cell Biology 2022Quote: ... and antisense primer (5’-GGA GAA AGG CAC CTG CCA GGC CTC AAC-3’) by site-directed mutation according to manufacturer’s protocol (QuikChange II XL Site-Directed Mutagenenesis Kit, 200521; Stratagene, LA Jolla, CA). The primers were designed to delete (in frame ...
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bioRxiv - Neuroscience 2020Quote: ... the sample quality was checked with both the Qubit 3 Fluorometer and the High Sensitivity D1000 ScreenTape assay (Agilent Technologies #5067-5584). The libraries were equimolarly pooled ...
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bioRxiv - Biochemistry 2021Quote: ... The digestion and desalting (total time 3 min) were driven by 0.4% formic acid (FA) in water pumped by 1260 Infinity II Quaternary pump (Agilent Technologies, Waldbronn, Germany) at a flow rate of 100 μL·min−1 ...
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bioRxiv - Cancer Biology 2022Quote: ... slices were then incubated with anti-cleaved caspase 3 antibody or anti-Ki67 antibody (Cell Signalling) and further processed with secondary antibody (LSAB2 horseradish peroxidase kit; Dako, Copenhagen, Denmark).
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bioRxiv - Immunology 2019Quote: A deleted version of pNL4-3 was constructed (18) that lacks the entire Gag and Protease region (Stratagene Quick-Change XL kit) replacing this region with a BstE II (New England Biolabs ...
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bioRxiv - Biochemistry 2020Quote: ... Thirty-six metabolites were from a list of potential biotransformation products directly derived from HGA and compiled using the Biotransformation Mass Defects application (Agilent; Appendix 3). This tool provides a list of potential metabolic biotransformation products covering both phase I (n=19 ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed with Bond Wash buffer 3 x 30s and then incubated with either mouse anti-CD68 (M0814, 1:100, Dako, Carpenteria, CA), mouse anti-βIII-Tubulin (G7121 ...
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bioRxiv - Cancer Biology 2021Quote: ... endogenous peroxidase activity was blocked by incubation with 3% H2O2 / Methanol for 10 minutes followed by incubation with Dako Dual Endogenous Enzyme Block reagent (Agilent Dako, S2003) for 30 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... E706A/E1015A and E437A/E706A/E1015A=EallA) were obtained by site-directed mutagenesis of pCS2-3×HA-NFATc3 using the QuickChange® II XL kit (Agilent Technologies) using the indicated primers ...
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bioRxiv - Biochemistry 2023Quote: Prior to LCMS analysis the TIMSTOF SCP was freshly tuned using the OTOF Control engineering software with an infusion of 3 μL/min of low-concentration tuning mix (Agilent, G1969-85000). Autotuning was performed for sensitivity and resolution tuning was manually checked ...
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bioRxiv - Biochemistry 2023Quote: Prior to LCMS analysis the TIMSTOF SCP was freshly tuned using the OTOF Control engineering software with an infusion of 3 μL/min of low-concentration tuning mix (Agilent, G1969-85000). Autotuning was performed for sensitivity and resolution tuning was manually checked ...
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bioRxiv - Systems Biology 2023Quote: ... Membranes were washed 3 times with TBST for 10 min at room temperature and incubated with horseradish peroxidase coupled secondary antibodies (Dako, P0448/P0447) at room temperature for 1 h (1:2000 in 0.3% (w/v ...
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bioRxiv - Cancer Biology 2023Quote: ... The cleared lysates were loaded on wells of 96-well single-use filter micro-plates with 3 µm glass fibers and 25 µm polyethylene membranes (Agilent, 204495-100) containing the HB95 cross-linked beads ...
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bioRxiv - Genomics 2023Quote: ... cDNA was diluted to approximately 3 ng/μL and run on the 2100 Agilent Bioanalyzer using the High Sensitivity DNA Kit (Agilent 5067-4626). The measured fluorescence units in Fig 1b have been corrected for the dilution factor.
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bioRxiv - Cancer Biology 2023Quote: ... treated with 3% H2O2 to block endogenous peroxidase then incubated in a steamer for 30 minutes in Target Retrieval Solution (Dako, #S1699, #S2367) for antigen retrieval ...
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bioRxiv - Neuroscience 2023Quote: ... samples were centrifuged at 20.800 xg for 2 min and 100 µl sample was injected on a SEC-HPLC column (Bio SEC-3 300 Å, Agilent, USA) using an Agilent 1260 Infinity II system (Agilent ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells were permeabilized with 0.1% Triton x-100 (Fluka, 93426) and blocked with the blocking solution containing 3% Goat serum (Dako, Cat. no. X0907), 0,1% sodium azide (the healthcare business of Merck KGaA ...
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bioRxiv - Cell Biology 2023Quote: ... TMEM24(ΔBS + 5S → E)-eGFP was generated using site-directed mutagenesis to remove a portion of the C-terminus of TMEM24(5S → E)-eGFP including the first 3 β-strands of the β-sheet band 4.1 interacting domain (Quik-Change II XL; Agilent Technologies).TMEM24(5S → E)-eGFP which has been previously described (Sun et al. ...
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bioRxiv - Systems Biology 2023Quote: ... Membranes were washed 3 times with TBST for 10 min at room temperature and incubated with horseradish peroxidase coupled secondary antibodies (Dako, P0448/P0447) at room temperature for 1 h (1:2000 in 0.3% (w/v ...
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bioRxiv - Immunology 2021Quote: ... a monoclonal mouse antibody against the intracellular tail of human HLA-DR α chain was used (Clone TAL.1B5, # M0746, Agilent, Santa Clara, CA, USA). Detection of MHC-I on the surface of cells was performed using a monoclonal mouse antibody against devil beta-2-microglobulin (B2M ...
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bioRxiv - Immunology 2021Quote: ... The library was hybridized to biotinylated cRNA oligonucleotide baits from the SureSelect Human All Exon kit V6+somatic probe sets (Agilent Technologies Inc., Santa Clara, CA), and amplified for 12 cycles ...