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Citations for Agilent :
1351 - 1400 of 1509 citations for Recombinant Human CD22 protein His tagged APC labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... About 1.0 µg of protein for each sample was injected, bound to a PLRP-S column (2.1mm x 50mm, 5μm, 300Å) (Agilent Technologies), desalted ...
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bioRxiv - Microbiology 2021Quote: ... The myr(–)MA and myr(–)MA99 proteins were overexpressed in Escherichia coli BL21 Codon Plus-RIL cells (Agilent Technologies). Cells were grown in LB broth supplemented with 100 mg/L Ampicillin at 37 °C ...
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bioRxiv - Immunology 2022Quote: ... the separation of digested protein samples was performed on an Agilent 1290 Infinity HPLC system (Agilent Technologies, Waldbronn, Germany) coupled to a Q Exactive™ HF (Thermo Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... The digested proteins were desalted using AssayMap C18 cartridges mounted on a BRAVO liquid handling system (Agilent, Columbia, MD), and the organic solvent was removed in a SpeedVac concentrator prior to LC-MS/MS analysis.
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bioRxiv - Biochemistry 2021Quote: ... These data were then normalized to the corresponding DNA/protein/cell number in the Wave data analysis software (Agilent) and exported to Microsoft Excel.
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bioRxiv - Immunology 2021Quote: ... all sections were blocked in a ready-to-use serum free protein blocking solution (Dako/Agilent, Santa Clara, CA) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: ... all sections were blocked in a ready-to-use serum free protein blocking solution (Dako/Agilent, Santa Clara, CA) for 10 minutes at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... The final protein concentration was measured using ultraviolet-visible (UV-vis) spectroscopy on a Cary 100 system (Agilent Technologies). All proteins were aliquoted ...
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bioRxiv - Biochemistry 2022Quote: ... 100 μl of each protein sample at 1.7-36 mg/ml were injected onto a BioSEC3-300 column (Agilent), run at a 0.3 ml/min flow rate ...
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bioRxiv - Biochemistry 2022Quote: ... The protein sample is scanned at room temperature using fluorometer (Cary Eclipse Fluorescence Spectrophotometer, Agilent, Santa Clara, CA, USA). During the scan ...
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bioRxiv - Cancer Biology 2022Quote: ... The nonspecific-binding sites on sections were then blocked using Protein Block Serum-Free reagent (Dako North America Inc.) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2022Quote: ... The tetrameric detection reagents were generated immediately prior to use by combining biotinylated FcɣR proteins (Ackerman Laboratory) with a 1/4th molar ratio of Streptavidin-Allophycocyanin (ProZyme) and mixing by inversion for 10 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Following permeabilization the cells were washed three times with PBS then blocked with Protein Block (Aligent/Dako, X090930-2). Primary antibodies (ADAR1 (Santa Cruz ...
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bioRxiv - Immunology 2024Quote: ... all sections were blocked in a ready-to-use serum free protein blocking solution (Dako/Agilent, Santa Clara, CA) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2024Quote: ... all sections were blocked in a ready-to-use serum free protein blocking solution (Dako/Agilent, Santa Clara, CA) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: Lung homogenate protein concentrations were determined using a Bio-Rad DC protein assay performed according to the manufacturer’s protocol and read on a Synergy H1 plate reader (Agilent). Concentrations were calculated by extrapolation from a BSA standard curve using Gen5 v.3.00 (Agilent) ...
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bioRxiv - Physiology 2023Quote: ... Protein concentration in the host fraction was measured on a spectrophotometer (BioTek PowerWave XS2, Agilent, Santa Clara CA, USA) using Coomassie Plus Bradford assay reagent (Pierce ...
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bioRxiv - Synthetic Biology 2023Quote: Protein concentrations were obtained by measuring absorbance at 488 nm on a Cary 60 UV-Vis spectrophotometer (Agilent Technologies) and calculating concentrations using Beer’s Law ...
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bioRxiv - Biophysics 2023Quote: ... A non-cleavable T855G mutant of this Lphn3 GAIN domain fusion protein was generated using QuikChange II XL (Agilent) site-directed mutagenesis with primers 5’-ATG CAG CTG TAA TCA CCT GGG CAA CTT TGC TGT CCT GAT G -3’ and 5’-CAT CAG GAC AGC AAA GTT GCC CAG GTG ATT ACA GCT GCA T -3’.
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bioRxiv - Cancer Biology 2023Quote: ... The protein (10 μL) was loaded by an LC system (Agilent 1290 Infinity II, Agilent Technologies, Santa Clara, CA) on a HPLC column (PLRP-S 1000 Å ...
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bioRxiv - Biochemistry 2023Quote: ... The plasmid carrying the WT Cagα protein pHTCagα was amplified using the QuikChange II site-directed mutagenesis kit (Agilent) using 33 bp primers (suppl ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were incubated for 30 minutes at room temperature prior to injection onto an HPLC (Agilent, 1260 Infinity II). 5 μL of protein complex was injected onto a MAbPac 4 x 300 mm SEC column with 5 µm particle size and 300 Å pore size ...
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bioRxiv - Physiology 2024Quote: ... Secreted protein in cell culture media was enriched using StrataClean Resin (Agilent, 10 µl per 5 ml of media) as previously described37 ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were revealed by chemi-luminescence (Super Signal West Dura Substrate, Pierce) using a secondary peroxydase-conjugated antibody (Dako) at a dilution of 1:10000 ...
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bioRxiv - Immunology 2024Quote: ... the slides were washed in 1× PBS and then exposed to Dako Serum-free Protein Block solution (Agilent, USA) for 45 min at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... fixed tissues were embedded in paraffin and 4 mm sections were histologically processed for hematoxylin-eosin staining and immunohistochemistry using anti-human Ki67 antibody (DAKO #M7240, 1:75, 30 min at 37°C). All these procedures were performed using standardized protocols by Atrys Health S.A.
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bioRxiv - Microbiology 2020Quote: ... The 75th amino acid in the PB1-F2 protein was changed to a histidine (H) via site directed mutagenesis (QuikChange Lightning, Agilent) to produce the PB1-F2-75H plasmid ...
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bioRxiv - Cell Biology 2020Quote: ... MBP-Tlg21-327 and the co-expressed Vps45–Tlg2 proteins were expressed and purified in a similar manner with the following modifications: protein was overexpressed in BL21-Codon Plus (Agilent); after IPTG addition the cells were grown at 16°C ...
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bioRxiv - Cell Biology 2020Quote: Samples from all biological replicates were first sent to the Stanford University Protein and Nucleic Acid Facility (Stanford, CA) for quantification and quality analysis using a 2100 Bioanalyzer (Agilent). Samples were then sent to Novogene Corporation Inc ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were washed for 5 minutes using 1X Wash Buffer and then blocked by adding 8 drops of Protein Block Serum-Free (Agilent). Primary antibodies were diluted in diluent (Dako ...
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bioRxiv - Molecular Biology 2021Quote: Affinity purification and digestion of biotinylated proteins were carried out in an automated fashion in a Bravo AssayMap platform (Agilent) using AssayMap streptavidin cartridges (Agilent) ...
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bioRxiv - Immunology 2019Quote: Purified recombinant His-tagged C-terminal MSP1 protein (amino acids 4960 to 5301) (Ndungu et al., 2009) was biotinylated and tetramerized with streptavidin-PE (Prozyme), as previously described (Krishnamurty et al. ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The multiple charged peaks of the antibody were deconvoluted using the Agilent MassHunter Bioconfirm software (deconvolution for protein, Agilent technology) with the deconvolution range from 20 kDa to 160 kDa ...
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bioRxiv - Physiology 2021Quote: ... Samples were washed with PBS twice and were either blocked for 10 min at room temperature (Dako protein block #X0909), or with goat block (GB ...
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bioRxiv - Physiology 2021Quote: ... Samples were washed with PBS twice and were either blocked for 10 min at room temperature (Dako protein block #X0909), or with goat block (GB ...
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bioRxiv - Microbiology 2020Quote: Proteins were detected using the following primary antibodies: anti-enterovirus VP1 clone 5-D8/1 antibody purchased from Dako (Denmark); Mouse anti-Ub ...
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Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasisbioRxiv - Microbiology 2021Quote: ... Seahorse data were normalized to total amount of protein (μg) and mitochondrial respiration parameters were analyzed using Wave Desktop 2.6 software (Agilent Technologies).
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bioRxiv - Molecular Biology 2020Quote: ... The free HSF2BP protein as well as the complex between ARM and F15X were analyzed using a Bio SEC-3 column (Agilent) equilibrated in 25 mM Tris-HCl buffer ...
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bioRxiv - Neuroscience 2020Quote: Site-directed insertions were made in GluN1 (GluN1-a) (NCBI Protein database accession no. P35439) or GluN2A (Q00959) subunits with the QuikChange site-directed mutagenesis kit (Agilent) with XL1-Blue super-competent cells ...
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bioRxiv - Cell Biology 2020Quote: The cells were fixed with 4% paraformaldehyde at 4°C for 5 min and permeabilized with 0.1% Triton X-100 at room temperature for 20 min in the presence of a protein-blocking solution consisting of PBS supplemented with 5% normal goat serum (Agilent Technologies ...
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bioRxiv - Plant Biology 2022Quote: His6-MBP-PFA-DSP protein fusions or free His8-MBP were expressed in Escherichia coli BL21 CodonPlus (DE3)-RIL cells (Stratagene). Overnight bacterial cultures were inoculated 1:1000 into fresh 2YT medium (1.6 % tryptone ...
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bioRxiv - Cancer Biology 2022Quote: ... Sections were washed with TBST (Tris Borate Saline Tween-20) and then blocked with Protein Block Serum-Free (Ref: No X0909, Dako) for 10 min ...
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bioRxiv - Biochemistry 2020Quote: ... A plasmid coding for the fusion protein with a K1432A substitution in the RemA CTD was made by the QuikChange mutagenesis (Agilent) using primers PG007 and PG008.
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bioRxiv - Microbiology 2020Quote: ... A construct expressing the MBP-MogRQN→AA protein was created by site-directed mutagenesis of pMAL-p5x-mogR using the QuikChange II Site-Directed Mutagenesis Kit (Stratagene) with the same primers as for pHT304-Pxyl-MogRQN→AA ...
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bioRxiv - Biochemistry 2020Quote: ... and the fluorescence signals corresponding to temperature-dependent protein unfolding were measured using a Real-Time PCR Mx3005p machine (Stratagene). The melting temperature calculation was performed as described previously46.
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bioRxiv - Biophysics 2022Quote: 30 µl EPYC1 and 30 µl Rubisco proteins were mixed in the cuvette for measurement in the Cary 60 UV-Vis spectrophotometer (Agilent). After 10 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... and the fluorescence signals corresponding to temperature-dependent protein unfolding were measured using a Real-Time PCR Mx3005p machine (Stratagene). Melting temperature shifts were calculated using the previously described method61.
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bioRxiv - Neuroscience 2021Quote: ... to test the presence of electrical coupling between THINs and NGF interneurons we used double transgenic TH–Cre crossed with (BAC) transgenic mice that express the humanized Renilla green fluorescent protein (hrGFP) (Stratagene) under the control of the mouse NPY promoter (NPY-GFP ...
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bioRxiv - Pathology 2020Quote: ... Sections were allowed to thaw at RT and thereafter blocked for > 60 min at RT with blocking-buffer (serum-free protein blocking solution, DAKO), supplemented with 0.2% Triton X-100 (Sigma Aldrich) ...
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bioRxiv - Biophysics 2019Quote: ... Major interfering proteins were removed from the serum by the Multiple Affinity Removal Spin Cartridge HSA/IgG (Agilent Technologies, USA) as described into manual ...