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Citations for Agilent :
1151 - 1200 of 1509 citations for Recombinant Human CD22 protein His tagged APC labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Anti-CD61 immunohistochemistry (Monoclonal Mouse Anti-Human, Citrate Buffer HIER, dilution 1:75, Clone Y2/51, Dako Omnis, Code Number: M0753) was also employed to evaluate the presence of platelet-enriched microthrombi ...
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bioRxiv - Cancer Biology 2022Quote: Genome-wide copy number variations were assessed using the SurePrint G3 Human aCGH 4×180K Microarray (Agilent Technologies, Santa Clara, CA), according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2024Quote: ... genomic DNA was fragmented into 180–280 bp by sonication and subjected to library preparation using the Agilent SureSelect Human All Exon V6 Kit (#5190-8864, Agilent Technologies). The enriched libraries underwent paired-end 150bp sequencing on the Illumina HiSeq 2000 platform.
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bioRxiv - Cancer Biology 2024Quote: ... 1 μg of the library was hybridized to the Agilent SureSelect XT Human All Exon V4 probes (Agilent Technologies 5190-4634). The targeted exon fragments were captured by Streptavidin beads (Fisher 65602) ...
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bioRxiv - Biophysics 2024Quote: The recombinant human Cα subunit of cAMP-dependent protein kinase with the Phe to Ala mutation in position 100 (PKA-CF100A) was generated from the human PKA-Cα wild-type using Quik-Change Lightning mutagenesis kit (Agilent genomics). The key resource table lists the PCR primers used to modify the pET-28a expression vector encoding for the wild-type human PKA-Cα gene (PRKACA – uniprot P17612 ...
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bioRxiv - Cancer Biology 2023Quote: ... Gene expression profiling was performed using a low-input QuickAmp labeling kit and human SurePrint G3 8×60K microarrays (Agilent Technologies), as previously described (Sulpice et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... Flag-tagged wt and Ex19Del human EGFR constructs in the lentiviral VIRSP vector were generated by PCR with Herculase II Fusion DNA Polymerase (Agilent technologies) from EGFR WT (a gift from Matthew Meyerson ...
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bioRxiv - Pathology 2023Quote: ... with the mouse Prnp encoding the 3F4 epitope (109M, 112M human numbering) was used as a template for site-directed mutagenesis (QuikChange Site Directed Mutagenesis kit™) (Agilent). PrP-deficient RK13 cells (ATCC ...
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bioRxiv - Cell Biology 2023Quote: ... Samples from the primary human monolayer experiment were fixed and immunostained for insulin with guinea pig anti-insulin primary antibody (Dako A0564) and Alexa-Fluor 568 goat anti-guinea pig secondary antibody (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: Deletions of SP1 sites on human MGMT promoter were performed using Quick-Change XL Site-Directed Mutagenesis kit (Agilent Technologies, USA).34 The mutated promoters were further cloned into pGL3 basic vector ...
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bioRxiv - Pathology 2023Quote: ... consecutive 4-μm slices of human aspirated thrombi were immunohistochemically stained using antibodies against CD34 (mouse monoclonal, clone QBEnd10; Dako/Agilent), FXI (LSBio) ...
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bioRxiv - Biochemistry 2023Quote: ... An alanine mutant library of the SNAP-tagged human truncated mGlu5 receptor (mGlu5-Δ856, designated as WT in the following experiments) was generated using Quick-change strategy (Agilent technologies) and verified by sequencing (Eurofins Genomics)11 ...
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bioRxiv - Neuroscience 2024Quote: ... Then membranes were probed with the following antibodies: anti-human tau polyclonal antiserum KJ9A (1:5,000; A0024, Dako Cooperation, Carpinteria, CA) and rabbit anti-GAPDH antiserum (1:5,000 ...
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bioRxiv - Neuroscience 2024Quote: The mutant PDI-Ala256 protein was prepared from the full-length wild-type human PDI cDNA using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies). For protein purification ...
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bioRxiv - Cell Biology 2024Quote: ... Mutant human PINK1 constructs were generated by QuickChange XL site-directed mutagenesis according to the manufacturer’s guidelines (Agilent Technologies #HPA 200516). The following constructs were made ...
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bioRxiv - Cell Biology 2020Quote: ... This step was directly followed by an incubation with Protein Block Serum Free (Dako X0909) for 30 minutes to block the sticky sites ...
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bioRxiv - Cell Biology 2022Quote: ... The expression of the GST-GAP fusion protein in E.coli strain BL-21-RILP (Stratagene) and its purification using Glutathione Sepharose 4B were performed as described previously (Jung et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... Each section was treated with 4 drops of serum-free protein block (Agilent cat # X0909) for 20 minutes at room temperature ...
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bioRxiv - Biochemistry 2019Quote: ... Temperature-dependent protein unfolding profiles were measured using a Real-Time PCR Mx3005p machine (Stratagene). The data evaluation and melting temperature calculation were performed as described previously52’53.
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bioRxiv - Neuroscience 2019Quote: ... and rabbit anti-S100 calcium-binding protein B β-subunit (S100-β) polyclonal antibody (Agilent Dako ...
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bioRxiv - Cancer Biology 2021Quote: Ligand binding to protein was detected using DSF on an MX3005P qPCR system from Agilent Technologies as described elsewhere.37 Briefly ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: The nsp14 and nsp10 proteins were expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL (Stratagene). The bacteria were grown in Luria–Bertani medium supplemented with 50 mg/mL kanamycin at 37 °C to an OD600 of 0.6 ...
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bioRxiv - Cancer Biology 2019Quote: ... sections were incubated for 5 min at room temperature with Protein Block Serum-Free (Dako). The tissue sections were then incubated with the anti-249T-P pAbs or TpMab-1 mAb (1 ...
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bioRxiv - Microbiology 2019Quote: ... and the vesicular stomatitis virus G protein (VSV-G) expression vector pVPack-VSV-G (Stratagene). After 72 hours ...
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bioRxiv - Physiology 2019Quote: ... Slides were incubated with DAKO Protein Block Serum-Free (Agilent Technologies, Inc, Santa Clara, CA) at room temperature for 10 minutes and then with the primary antibodies diluted in Dako Antibody Diluent (Agilent Technologies ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were expressed in BL21-CodonPlus (DE3)-RIL Escherichia coli (Agilent Technologies, catalog no. 230245) at 37°C with 0.25 mM IPTG (Isopropyl β-D-1-thiogalactopyranoside ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Samples were washed and blocked for 10 min (Dako protein block; Agilent, Santa Clara, CA) before being treated with primary antibodies overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... Plasma samples were depleted of high- and mid-abundant proteins using immune-depletion columns (Agilent Multiple Affinity Removal Column MARS3 (Agilent 5188-5218 ...
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bioRxiv - Plant Biology 2023Quote: ... Substrate proteins were induced and expressed in Escherichia coli BL21 CodonPlus(DE3)-RIL cells (Stratagene) over night at 18°C after induction with 1 mM IPTG ...
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bioRxiv - Neuroscience 2022Quote: ... The primary antibodies used in this study were glial fibrillary acidic protein (GFAP; Source: Dako; Host ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated at 4°C overnight with primary antibody in Protein Block (Dako, CA). Sections were rinsed 4x in PBS ...
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bioRxiv - Systems Biology 2024Quote: ... VA) or glial fibrillar acidic protein (GFAP; 1:1000; G3893-100uL; Dako, Santa Clara, CA). For Iba1 staining ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were visualized with 3,3′-diaminobenzidine (DAB) (K346811-2, Agilent Technologies, Inc., Santa Clara, CA) or Vina Green (BRR807AS ...
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bioRxiv - Cancer Biology 2024Quote: ... Each cycle included a 5 min blocking step with serum free Protein Block (Agilent, #X0909), primary antibody incubation for 30 min ...
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bioRxiv - Immunology 2023Quote: ... VP protein masses were then measured using an in-line 6545XT QTOF system (Agilent Technologies) using the following parameters ...
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bioRxiv - Cancer Biology 2023Quote: ... and treated for a further 10 min with serum-free protein block solution (Agilent; #X0909). Primary antibodies were diluted in antibody diluent (Agilent ...
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bioRxiv - Neuroscience 2023Quote: ... All other proteins were expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL cells (Agilent Technologies) in LB medium at 16 □ overnight ...
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bioRxiv - Neuroscience 2023Quote: ... and 200 μL of blocking solution containing 50% serum-free protein block (X090930-2, Dako) and 50% normal horse serum (2.5% blocking solution ...
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bioRxiv - Biochemistry 2023Quote: ... Secreted proteins were captured by addition of 100:1 (v/v) secretome:Strataclean resin (Agilent Technologies) (∼2 ml ...
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bioRxiv - Cell Biology 2023Quote: ... Tissue sections were blocked for 1 h at RT with protein blocking solution (#X0909, Dako). After blocking ...
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bioRxiv - Cell Biology 2023Quote: Bsp1 proteins were expressed overnight at 20°C in BL21 pLysS Gold competent cells (Agilent) using autoinduction LB medium (Formedium) ...
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bioRxiv - Cell Biology 2020Quote: ... EndoC-βH1 cells were incubated with prediluted antibody polyclonal Guinea Pig anti-Human Insulin (cat. IR002 - Agilent Technologies, Santa Clara, CA, USA) for 1h at RT ...
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bioRxiv - Genetics 2021Quote: ... and 5081) were used for whole-exome sequencing prepared using the SureSelect Human All Exon V7 probe set (Agilent, Design ID S31285117) per the manufacturers protocol ...
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bioRxiv - Neuroscience 2021Quote: ... RNA qualities of the human RNA samples (RNA Integrity Number; RIN score) were assayed with high sensitivity RNA Screen Tape (Agilent 5057-5579) and shown in Table S4 ...
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bioRxiv - Immunology 2021Quote: ... and bound serum IgG was detected by 70 µL/well of 1:3000 diluted rabbit anti-human IgG antibody linked to horseradish peroxidase (Agilent, P021402-2) incubated for 2h at RT ...
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bioRxiv - Genomics 2021Quote: ... Each sample was prepared according to Agilent library preparation protocols (Human All Exon 50 Mb kit; Agilent Technologies, Santa Clara, CA, USA). Libraries were subjected to paired-end sequencing on an Illumina Hiseq 2000 and 2500 instrument (Illumina ...
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bioRxiv - Cancer Biology 2020Quote: ... and cRNA was hybridized with ArrayXS Human (Oaklabs, Germany) at 65 °C for 17 hours using the Agilent Gene Expression Hybridization Kit (Agilent Technologies, USA), washed once with Agilent Gene Expression Wash Buffer 1 for one minute at room temperature ...
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bioRxiv - Cell Biology 2021Quote: Point mutations were introduced into the wild-type human Myo1e in the pEGFP-C1 vector using QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent Technologies #210519) [7 ...
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bioRxiv - Immunology 2021Quote: ... Paraffin sections (4µm thick) of FFPE thymus and lymph node tissues (NMR, mouse, and human control) were stained for cytokeratin (AE1/AE3, Dako GA05361-2) on a Dako Omnis autostainer with pressure cooker antigen retrieval (TrisEDTA ...
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bioRxiv - Cell Biology 2021Quote: ... Entry clones for other mutant dynamin 2 were prepared by introducing corresponding mutations into the Entry clone of wild type human dynamin 2 using QuikChange Lightning Site-directed Mutagenesis kit (Agilent Technologies, 210518) following manufacturer’s instruction ...