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Citations for Agilent :
1101 - 1150 of 1509 citations for Recombinant Human CD22 protein His tagged APC labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2020Quote: ... Affinity purification of biotinylated proteins was carried out in a Bravo AssayMap platform (Agilent) using AssayMap streptavidin cartridges (Agilent) ...
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bioRxiv - Developmental Biology 2019Quote: ... then blocked in serum-free protein blocking solution (Agilent Dako, Santa Clara, CA, USA) for 1hr at room temperature ...
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bioRxiv - Developmental Biology 2019Quote: ... then blocked in serum-free protein blocking solution (Agilent Dako, Santa Clara, CA, USA) for 1hr at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... All Mtr4 proteins were recombinantly expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL (Stratagene) using an autoinduction protocol (Studier ...
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bioRxiv - Biochemistry 2021Quote: ... The purified S protein was separated by an SEC column (BioSEC-3, Agilent, USA) connected to an HPLC system (Analytical HPLC 1260 LC system ...
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bioRxiv - Biochemistry 2021Quote: The KlNmd4 protein was expressed in Escherischia coli BL21(DE3) Gold strain (Agilent technologies) using pMG897 plasmid and 1 L of auto-inducible terrific broth media (ForMedium AIMTB0260 ...
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bioRxiv - Immunology 2021Quote: ... Non-specific protein binding was blocked by incubation with 10% normal goat serum (DAKO). The nonspecific binding of antibodies was blocked using 10% normal goat serum (DAKO ...
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bioRxiv - Neuroscience 2022Quote: ... glial fibrillary acidic protein (GFAP) staining was performed (rabbit-anti-GFAP primary antibody, DAKO Z0334 ...
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bioRxiv - Bioengineering 2024Quote: ... peroxidase blocking solution and serum-free protein blocking solution were purchased from Dako (DNK). The O.C.T ...
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bioRxiv - Neuroscience 2024Quote: ... sections were blocked and permeabilized in tandem using serum-free protein block (Dako, S0909) containing 0.3% Triton-X 100 at RT for 1h ...
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bioRxiv - Systems Biology 2022Quote: ... proteins were desalted using solid phase extraction C18 cartridges (Agilent, 5 μL bed volume). Then ...
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bioRxiv - Biophysics 2023Quote: dCas9-Cys protein expression was induced in BL21-CodonPlus(DE3)-RIL cells (Agilent #230245) transformed with plasmid 10xHis-MBP-TEV-S ...
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bioRxiv - Biophysics 2023Quote: ... cerevisiae Cdc6 protein expression was induced in BL21-CodonPlus(DE3)-RIL cells (Agilent #230245) transformed with pGEX-6P-1 wt GST-cdc6 using 400 µM IPTG for 16 h at 16 °C ...
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bioRxiv - Pathology 2023Quote: ... In addition to blocking unspecific antibody binding using Serum-Free Protein Block (X0909, Agilent) for 10 minutes ...
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bioRxiv - Immunology 2023Quote: ... proteins in the supernatant were precipitated with 1% (v/v) StrataClean resin (Agilent Technologies) overnight at 4°C ...
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bioRxiv - Systems Biology 2023Quote: ... Affinity purification of biotinylated proteins was carried out in a Bravo AssayMap platform (Agilent) using AssayMap streptavidin cartridges (Agilent) ...
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bioRxiv - Physiology 2023Quote: ... the sections were blocked using Protein Block Serum-Free Blocking (X0909, Agilent, Santa Clara) and incubated with a primary antibody targeting mouse α-SMA (1:300 ...
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bioRxiv - Immunology 2023Quote: ... and rabbit polyclonal antibodies against CD-3 protein (Dako, Glostrup, Denmark; cat. no. A0452).
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bioRxiv - Cancer Biology 2023Quote: ... The protein expression was detected using the liquid DAB+ substrate chromogen system (DAKO, #K3468).
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bioRxiv - Plant Biology 2024Quote: ... protein or peptide samples were first desalted and concentrated onto a µC18 Omix (Agilent) before analysis.
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bioRxiv - Cell Biology 2020Quote: ... EndoC-βH1 cells were incubated with antibody polyclonal Guinea Pig anti-Human Insulin (cat. A21435 - Agilent Technologies, Santa Clara, CA, USA) diluted 1:1740 in BSA 1% in PBS without Ca2+ and Mg2+and with monoclonal mouse anti-Human ACE2 (cat ...
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bioRxiv - Neuroscience 2020Quote: ... Neurons were treated with AEVs from AD and normal participants for 1 hour and then subjected to fluorescent immunolabelling for MAC using a mouse anti-human C5b-9 antibody (1 μg/mL; RRID: AB_2067162, cat. no. M0777, Dako, Carpinteria, CA) targeting an epitope in the poly-C9 component of MAC in association with C5b ...
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bioRxiv - Immunology 2022Quote: ... tissues fixed for 24-30 hours in 10% neutral-buffered formalin (NBF; 3.7% formaldehyde) as previously described (Wiarda et al., 2020) with polyclonal rabbit α-human CD3ε antibody (Dako A0452) and polyclonal goat α-rabbit horseradish peroxidase (HRP)-conjugated antibody (Dako K4003) ...
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bioRxiv - Immunology 2019Quote: ... the wells were washed as before and incubated with 50 µl/well polyclonal rabbit anti-human IgG conjugated with horseradish peroxidase (Dako, Denmark) at 0.5 µg/ml for another hour at room temperature ...
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bioRxiv - Genomics 2021Quote: ... 1.65 μg cRNA from each labeling reaction was hybridized to the Agilent Whole Human Genome Oligo Microarray (Agilent Technologies, Tokyo, Japan). The Whole Human Genome Oligo Microarray is in a 4 x 44k slide format and each block represents more than 41,000 unique human genes and transcripts ...
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bioRxiv - Genetics 2021Quote: ... and total RNA (ribosomal RNA depleted, toRNA-seq) obtained from an adult human cortex [66-year-old female (540005, Agilent Technologies)] and an adult mouse cortex [eight-week-old (C57BL/6J ...
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bioRxiv - Cancer Biology 2021Quote: Comparative gene expression profiling of the prostate cancer cells starved for glutamine for 24 h or cultured with glutamine supplementation was performed using SurePrint G3 Human Gene Expression 8×60K v3 Microarray Kit (Design ID 039494, Agilent Technologies) according to manufacturer’s recommendations as described previously 4 ...
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bioRxiv - Genetics 2021Quote: ... Genomic DNA from each clone was analyzed by microarray-based comparative genomic hybridization using catalog whole-genome microarrays (SurePrint G3 Human CGH microarray, 8 × 60 k format, Agilent technologies) and/or by multiplex ligation dependent probe amplification using the SHOX SALSA MLPA Probemix (assay ID ...
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bioRxiv - Cell Biology 2021Quote: Paraffin-embedded islet grafts within the kidneys or intact mouse or human islets were stained for insulin using polyclonal guinea pig anti-insulin antibody (Dako, A0564), and apoptosis was measured using the DeadEnd Fluorometric terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL ...
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bioRxiv - Cancer Biology 2022Quote: ... Synthesis of cDNA from isolated RNA as well as a Universal Human Reference RNA that was used as negative control or for normalization (HREF, Stratagene/Agilent # 740000) was performed using an oligo-dT primed SuperScript III Reverse Transcriptase (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: Transcriptome analysis of primary human HSC was performed by OakLabs (Hennigsdorf, Germany) using Agilent Microarray XS (Agilent Technologies, Santa Clara, USA). Low Input QuickAmp Labeling Kit (Agilent Technologies ...
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bioRxiv - Genetics 2020Quote: Trio WES of 13 patients and their parents was performed using the SureSelect Human All Exon V5+UTR kit (Agilent technologies). In patient 4 ...
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bioRxiv - Biochemistry 2019Quote: ... carrying the human WT RAD51 (WT-pET11d) was used as a template for the PCR-mediated QuikChange site-directed mutagenesis (Agilent Technologies) to introduce F86E substitution (F86E-pET11d ...
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bioRxiv - Cancer Biology 2019Quote: ... was used for preparing the samples for sequencing and SSELXT Human All exon V6 +UTR probes (Agilent Technologies, California, United State) were used for the exome capture ...
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bioRxiv - Genomics 2019Quote: Paired-end DNA sequencing libraries of 28 individuals were generated using Aglilent SureSelect Human All ExonV6 kit (Agilent Technologies, CA, USA) by Novogene Co. ...
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bioRxiv - Cancer Biology 2021Quote: ... the slides were treated with 1:10 normal goat serum in PBS for 15 minutes and incubated for 60 minutes with 1:200 diluted Rabbit anti-human thyroglobulin (Dako, Denmark) or 1:400 diluted Rabbit anti-human calcitonin (Dako ...
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bioRxiv - Cell Biology 2020Quote: To identify the causative mutation in these families we sequenced the whole exome of the affected individuals with the SureSelect human AllExon 50Mb kit (Agilent Technologies) and sequenced on the HiSeq 2500 (Illumina ...
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bioRxiv - Zoology 2020Quote: ... hydrated through graded ethanol and incubated overnight at 4°C with a mouse anti-human CD68 clone KP1 monoclonal antibody (1:100, Dako, Denmark). Then ...
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bioRxiv - Cancer Biology 2021Quote: ... RNAs from 4 different experiments (biological quadruplicate) were extracted as described above and analyzed on SurePrint G3 Human Gene Expression 8×60K v2 Microarrays (#G4851B, Agilent Technologies) as previously described (36) ...
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bioRxiv - Neuroscience 2020Quote: ... Human α7-nAChR R208I-GFP and E211N-GFP were generated from pcDNA3.1-CHRNA7-mGFP and human α3-nAChR I284R-GFP and N287E-GFP were generated from human α3-nAChR-GFP by PCR based QuikChange® Site-Directed Mutagenesis Kit (Agilent) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Exome sequencing was performed on genomic DNA from Patients 1 and 2 and their parents using a SureSelect Human All Exon kit (Agilent Technologies) for targeted enrichment ...
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bioRxiv - Neuroscience 2021Quote: The optimal concentration was determined for each antibody: CD3 (polyclonal rabbit – anti-human CD3 IgG, cat. no. A045201-2, Agilent Technologies), 1:60 ...
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bioRxiv - Biophysics 2021Quote: Mutations were introduced into the human CFTR(E1371S)/pGEMHE and CFTR(D1370N)/pGEMHE sequences using the QuikChange II XL Kit (Agilent Technologies) and confirmed by automated sequencing (LGC Genomics GmbH) ...
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bioRxiv - Cell Biology 2020Quote: ... All hESC lines were analysed for their genetic content by array-based comparative genomic hybridization (Human Genome CGH Microarray 4×44K, Agilent Technologies) as previously described (Jacobs et al. ...
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bioRxiv - Microbiology 2021Quote: ... membranes were washed with 0.1% Tween-20 in PBS and incubated with anti-mouse or anti-human IgG (1:5000; Agilent Dako, Glostrup, Denmark), conjugated to horseradish peroxidase ...
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bioRxiv - Immunology 2021Quote: ... membranes were incubated for 45 minutes in 1% dried skim milk in PBS-T with rabbit anti-human C1q (1:300, Dako, A0136) and HRP-conjugated goat anti-rabbit IgG (1:10.000 ...
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bioRxiv - Cancer Biology 2020Quote: ... The library was then enriched by liquid phase hybridization using Agilent SureSelect XT Human All Exon v6 (Agilent Technologies, CA, USA) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2022Quote: ... hepaticus versus the tdTomato fluorescent protein (72 h transduction) was quantified in epithelial intestinal HT-29 cells using the Human GE 4x44K v2 Microarray Kit (Agilent Technologies) as we previously detailed [13 ...
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bioRxiv - Microbiology 2022Quote: ... Slides were then washed in PBS/0.05% Tween 20 and incubated with rabbit anti human VWF (A008229-2, DAKO/Agilent tech) 1/500 or rabbit IgG isotype control (AB-105-C ...
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bioRxiv - Microbiology 2023Quote: ... all tissue section slides were treated with a mouse monoclonal Anti-Human IgG p27KIP1 (Clone SX53G8.5 dilution 1:100; Code M7203; Dako Cytomation, Denmark) that cross-reacts with p27kip1 using a protocol described previously [24] ...