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Citations for Agilent :
1301 - 1350 of 6160 citations for Onion Yellow Dwarf Virus OYDV PCR Kits since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... libraries were quantified using a Qubit dsDNA HS DNA Kit (Thermo Fischer Scientific) and visualized on an Agilent 2100 Bioanalyzer using an Agilent High Sensitivity DNA Kit (Agilent Technologies). Libraries were sequenced in a HiSeq2500 (Illumina Inc. ...
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bioRxiv - Immunology 2024Quote: ... Mitochondrial respiration and glycolysis were determined by measuring OCRs and ECARs in the extracellular space with a Seahorse XF96 Extracellular Flux Analyzer with the XF Cell Mito Stress Test Kit and XF Glycolysis Stress Test Kit (Seahorse Bioscience) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: Glycolysis and Mitochondrial stress tests were performed on confluent HUVECs according to the manufacturer’s kits and protocols (Agilent Seahorse XF glycolysis stress test kit, Agilent Technologies, Inc.). 1 μM Oligomycin was used ...
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bioRxiv - Cancer Biology 2023Quote: ... and 10 mM glucose (for the Cell Mito Stress Test Kit) or 1 mM glutamine (for the Glycolysis Stress Test Kit) was used as the assay medium (103681-100; Agilent Technologies). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were quantified using Qubit dsDNA HS assay kit (Q32851, TFS) and quality and fragment distribution were examined with Agilent High Sensitivity DNA kit (5067-4626, Agilent Technologies). Sequencing was performed on the NextSeq500 (HighOutput SR75 ...
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bioRxiv - Cancer Biology 2024Quote: ... the quality of the libraries was assessed using Agilent High Sensitivity DNA Kit and Agilent Bioanalyzer 2100 DNA Kit (Agilent Technologies). Libraries were sequenced on an Illumina NovaSeq 6000 with the sequencing configurations recommended by 10X Genomics ...
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bioRxiv - Biophysics 2021Quote: ... The thermal variations were in the range 25-95°C in a stepwise increment of 1 °C/minute step and using a 96-well PCR plate (Agilent Technologies) sealed with optical quality sealing tape (Microseal® ‘B’ seal from BIO-RAD) ...
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bioRxiv - Biophysics 2021Quote: ... Site-directed mutagenesis was performed using custom-designed primers (Eurofins Genomics) and regular PCR with PfuUltra II Fusion HS DNA Polymerase (Agilent Technologies). Plasmids were linearized using EcoRI (for mASIC1a ...
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bioRxiv - Biochemistry 2021Quote: Impβ charge reversal point mutations were generated by a PCR-based protocol adapted from the QuikChange site-directed mutagenesis method (Agilent Technologies). Impβ mutant proteins were expressed and purified as the WT and their molecular masses verified by LC/ESI mass spectrometry (Table S5).
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bioRxiv - Biochemistry 2021Quote: ... and Rev charge-reversal point mutations were generated by a PCR-based protocol adapted from the QuikChange site-directed mutagenesis method (Agilent Technologies). Rev mutant proteins were expressed and purified as the WT and their molecular masses verified by LC/ESI mass spectrometry (Table S5).
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bioRxiv - Molecular Biology 2021Quote: ... the immunoprecipitated DNA and total DNA were quantified by Power SYBR Green PCR Master Mix in a Mx3000P Real-Time qPCR System (Agilent Technologies). The primers used for qPCR are listed in Sup ...
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bioRxiv - Genomics 2021Quote: ... All libraries were prepared in parallel with the pre-PCR purification steps carried out using a Bravo Workstation pipetting robot (Agilent Technologies).
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bioRxiv - Systems Biology 2021Quote: ... 607 steady-state LC and 208 migrated LC from mastectomy skin were converted into ‘STAMPs’ for PCR library amplification (High Sensitivity DNA Assay, Agilent Bioanalyser) and tagmentation (Nextera XT ...
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bioRxiv - Plant Biology 2021Quote: ... The L480P mutation in GL2 was generated by one-step PCR-based site-directed mutagenesis (Scott et al., 2002) using PfuUltra II Fusion HS DNA polymerase (Agilent Technologies) with primers listed in Supplemental Table 5 ...
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bioRxiv - Systems Biology 2021Quote: ... 700 steady-state LC and ~300 migrated LC from mastectomy skin were converted into ‘STAMPs’ for PCR library amplification (High Sensitivity DNA Assay, Agilent Bioanalyser) and tagmentation (Nextera XT ...
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bioRxiv - Microbiology 2021Quote: ... The total volume for the RT-PCR reaction was 50 μl using a High-Fidelity DNA Polymerase (Agilent, Santa Clara, USA), with MBtuni-12 and MBtuni-13 (5’-ACG CGT GAT CAG TAG AAA CAA GG-3’ ...
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bioRxiv - Genomics 2021Quote: ... All libraries were prepared in parallel with the pre-PCR purification steps carried out using a Bravo Workstation pipetting robot (Agilent Technologies). 9-12 libraries were sequenced as a multiplex on one Illumina HiSeq 2000 lane using 125bp paired-end read length.
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bioRxiv - Cancer Biology 2020Quote: ... QRT-PCR was performed in triplicate including a non-template control using the Mx3000P system (Agilent Technologies, Inc., Santa Clara, CA). Oligos (Invitrogen ...
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bioRxiv - Plant Biology 2022Quote: ... and gl2Amo mutants were generated by one-step PCR-based site-directed mutagenesis (Scott et al., 2002) using PfuUltra II Fusion HS DNA polymerase (Agilent Technologies). The Phusion® Site-Directed Mutagenesis Kit (Thermofisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... full-length cDNAs of the zebrafish banp gene were amplified with Polymerase Chain Reaction (PCR) and sub-cloned into pBluescript II SK(+) vectors (Stratagene/Agilent Technologies). cDNA fragments were amplified from mRNA with PCR using the primers for cenpt ...
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bioRxiv - Cell Biology 2022Quote: ... 0.0025 μl of a 1:40,000 diluted ERCC spike-in concentration stock and all cDNA was amplified with 22 PCR cycles before QC control with a Bioanalyzer (Agilent Biosystems). The libraries were sequenced on a HiSeq2500 at the National Genomics Infrastructure (NGI) ...
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bioRxiv - Plant Biology 2021Quote: ... Quantitative real-time PCR was performed with Thunderbird SYBR qPCR mix (Toyobo) in Stratagene MX3000P real-time qPCR system (Agilent Technologies). The transcript levels were calculated with standard curve method and normalized by that of internal control PROTEIN PHOSPHATASE 2A-3 (PP2A3)69 ...
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bioRxiv - Molecular Biology 2020Quote: ... The qPCR assays were carried out in a MX 3005 real-time PCR system (Agilent, Santa Clara, CA 95051, United States) using Brilliant III Ultra-Fast SYBR Green qPCR Master Mix (Agilent) ...
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bioRxiv - Bioengineering 2021Quote: The integrated sgRNA library was PCR amplified and separately barcoded for each collected population with Herculase II Fusion DNA Polymerase (Agilent 600677). Samples were then pooled and sequenced on an Illumina NextSeq flow cell with aligned read counts as follows for each screen:
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bioRxiv - Microbiology 2021Quote: ... qRT-PCR was performed with 4 μl Brilliant III Ultra-Fast SYBR QPCR as instructed by the manufacturer (Agilent Santa Clara). All primers for quantification of either expression of viral m/cRNAs or antiviral responses are shown below.
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bioRxiv - Pathology 2020Quote: ... and real-time RT-PCR was performed using Brilliant III Ultra-Fast SYBR Green QPCR Master Mix (Agilent Technologies, CA, USA). The synthetic gene-specific primers are listed in Table 1 ...
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bioRxiv - Microbiology 2020Quote: ... Abundances of bacterial and archaeal amoA genes and bacterial 16S rRNA genes were determined by quantitative PCR using a Mx3000P instrument (Agilent, CA). Primer combinations were AmoA-1F/ AmoA2R (Rotthauwe et al. ...
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bioRxiv - Immunology 2021Quote: ... PCR amplification verification and library validation was done using a TapeStation electrophoresis system and D1000 ScreenTape reagents (Agilent, Santa Clara, California). Libraries were quantified using a Qubit 4 Fluorometer and the Qubit dsDNA HS assay kit (ThermoFisher Scientific ...
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bioRxiv - Genomics 2021Quote: ... cDNA were amplified by 10 PCR cycles and purified before assessing their quality on the bioanalyzer (2100 high sensitivity DNA assay, Agilent Technologies). 150 pg of cDNA were used as input to prepare the libraries using the Nextera XT DNA kit (Illumina) ...
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bioRxiv - Evolutionary Biology 2021Quote: Evolved viral genomes of the passage 12 from each lineage and genotype were amplified by high-fidelity RT-PCR using the AccuScript Hi-Fi (Agilent Technologies) reverse transcriptase and Phusion DNA polymerase (Thermo Scientific ...
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bioRxiv - Molecular Biology 2021Quote: The full-length Fe-S cluster assembly protein SufB from the Mycobacterium tuberculosis H37Rv strain (Mtu FL-SufB) and its isolated intein (Mtu-SufB-I) genes were PCR-amplified using Pfu Ultra High-Fidelity DNA Polymerase (Agilent Technologies) from heat-killed Mtu genomic DNA ...
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bioRxiv - Genetics 2021Quote: ... All PCR amplification steps were performed using the high-fidelity Herculase II Fusion DNA Polymerase (Agilent, Santa Clara, CA, United States), and assembly of the recombinant vector was performed with the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... Libraries were enriched with 2 cycles of PCR then assessed for size distribution using the 4200 TapeStation High Sensitivity D1000 ScreenTape (Agilent Technologies) and quantified using the Qubit dsDNA HS Assay Kit (Thermo Fisher) ...
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bioRxiv - Biophysics 2020Quote: ... the chromosomal locus comprising the open reading frame slr1645 (Psb27) was amplified by PCR (Table S2) using Herculase II Phusion DNA polymerase (Agilent USA) and integrated at a neutral site within the Synechocystis genome between ORFs slr1169 and slr1285 (Fig ...
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bioRxiv - Cancer Biology 2020Quote: ... qRT-PCR was performed on 1:10 diluted cDNA using either SYBR-containing Brilliant III Ultra-Fast QPCR Master Mix (Agilent Technologies) and gene-specific primers ...
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bioRxiv - Cell Biology 2023Quote: ... Real-time quantitative PCR reactions using SYBRgreen fluorescent reagent and/or EvaGreen™ were achieved on the Stratagene Mx3000P system (Agilent Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... Flag-tagged wt and Ex19Del human EGFR constructs in the lentiviral VIRSP vector were generated by PCR with Herculase II Fusion DNA Polymerase (Agilent technologies) from EGFR WT (a gift from Matthew Meyerson ...
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bioRxiv - Cell Biology 2024Quote: ... Quantitative real-time PCR reactions employing SYBRgreen fluorescent reagent and/or EvaGreen™ were performed in the Stratagene Mx3000P system (Agilent Technologies ...
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bioRxiv - Bioengineering 2024Quote: ... 1 µL of eluted RNA and 5 µL RNA ScreenTape sample buffer was mixed in an 8-well PCR tube strip (Agilent Technologies) via vortexing for one minute (IKA MS3 Vortexer ...
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bioRxiv - Cell Biology 2024Quote: ... All the mutants described in this work were generated via PCR mutagenesis using cloned PFU polymerase (Agilent Technologies, Santa Clara, CA).
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bioRxiv - Cancer Biology 2024Quote: ... using miR30-adapted sequences (5-6 shRNAs per target) by PCR-cloning a pool of oligonucleotides synthesized on 55k customized arrays (Agilent Technologies) using a well-established system 84–87 ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR amplification of the eluted DNA was performed as described in the manufacturer’s protocol but with the addition of 2.5 µl Evagreen (20X) for visualization of the amplification curves on an AriaMx Real-time PCR system (Agilent Aria 1.7). The denaturation and annealing/extension steps of the PCR amplification were performed for around 12 cycles and stopped before the curves plateaued ...
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bioRxiv - Microbiology 2024Quote: ... 40x 10 sec 95°C and 30 sec 60°C with the Brilliant III Ultra-Fast qRT-PCR master mix (Agilent, 600884) were used according to manufacturer’s protocol.
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bioRxiv - Molecular Biology 2023Quote: ... Real-time PCR analysis was performed using Perfect Start Green qPCR Super Mix (+DyeII) (AQ602-24, TransGen, Beijing, China) and the AriaMx Real-Time PCR System (Agilent Technologies). See Table S2 for primer information.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... was used to synthesize cDNA and quantification of viral gene expression was performed on the Mx3005P Real-Time PCR System (Stratagene, USA), using Ultra SYBR Mixture (Low ROX ...
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bioRxiv - Immunology 2024Quote: ... 11 cycles of TCR target enrichment PCR 1 and 2 were performed and the resulting cDNA was quantified on an Agilent Bioanalyzer High Sensitivity chip (Agilent Technologies). TCR libraries were prepared and indexed with 9 cycles of amplification using the PN-220103 Chromium i7 Sample Index Plate ...
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bioRxiv - Cancer Biology 2023Quote: ... 5ng of cDNA was used for the qRT-PCR reaction using Brilliant III Ultrafast SYBR Green QPCR master mix (Agilent Technologies) on AriaMx qRT-PCR system (Agilent Technologies) ...
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bioRxiv - Plant Biology 2024Quote: ... was carried out in a 25 μL reaction volume using the Brilliant II SYBR Green PCR Master Mix (Stratagene, Agilent technologies). Primer concentrations were set at 0.2 μM ...
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bioRxiv - Microbiology 2022Quote: ... The ligation product was enriched with 15 PCR cycles and the final library was validated on an Agilent 2100 Bioanalyzer (Agilent Technologies) using the DNA 7500 kit (Agilent Technologies).
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bioRxiv - Microbiology 2022Quote: ... the gentamicin resistance marker (PflaB-aaC1) was amplified from pMH105 (20) by PCR and then TA-cloned into pSC-A-amp/kan (Agilent Technologies). Following confirmation of the TA clones ...