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Citations for Agilent :
51 - 100 of 1150 citations for Borrelia burgdorferi C p Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... was determined during basal and upon addition of oligomycin (1 µM), carbonylcyanide p-(trifluoromethoxy) phenylhydrazone (FCCP, 2 µM) and rotenone/antimycin (0.5 µM) using the Seahorse XFe24 bioanalyzer (Agilent). Data from each well was corrected to cell number using the Bio Tek Cytation 1 Cell Imaging Multimode Reader and presented as mean +/-SEM.
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bioRxiv - Neuroscience 2020Quote: ... A 10-minute protein block step with Serum-Free Protein Block (X0909, Agilent DAKO) to prevent non-specific binding was followed by a 1-hour incubation in primary antibody (pERK ...
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bioRxiv - Neuroscience 2020Quote: ... A 10-minute protein block step with Serum-Free Protein Block (X0909, Agilent DAKO) to prevent non-specific binding was followed by a 1-hour incubation in primary antibody (pERK ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein Blocking reagent (Dako, X0909) and Bloxall blocking solution (Vector ...
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bioRxiv - Cancer Biology 2020Quote: ... dry protein A cartridges (Agilent) were primed in PBS at 300 μL/min before loading 1 mg of antibody at 1 mg/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein Blocking reagent (Dako, X0909) and Bloxall blocking solution (Vector ...
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bioRxiv - Cancer Biology 2023Quote: ... blocked in Protein block (DAKO) for 10min at RT ...
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bioRxiv - Cell Biology 2023Quote: ... BSA and Protein block (Dako). Sections were stained with primary antibodies against GFP (ab290 ...
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bioRxiv - Cancer Biology 2022Quote: ... the samples were denatured in the presence of the specific probe at 75°C for 5 min and left overnight for hybridization at 37°C in a hybridizer machine (DAKO). The probes used detect copy number alterations in chromosomes 7 and 8 (KBI-20031 ...
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bioRxiv - Plant Biology 2023Quote: ... Temperature-dependent phase transition was detected by measuring the optical disturbance at 800 nm by increasing the temperature from 4 to 60°C (with a ramping rate of 0.5°C/min) using a spectrophotometer Cary 300UV-VIS (Agilent Technologies). Nitrogen gas purging was used to prevent the moisture from condensing on the cuvette surface.
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bioRxiv - Plant Biology 2024Quote: ... Fragmentation (at 60 °C) and hybridization (at 65° C for 16 hours) were done using the Gene Expression Hybridization kit of (Agilent Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... 30 s at 58 °C and 30 s at 72 °C were carried out on a Multiplex 3005 Plus (Stratagene/Agilent). The amplicon coordinates relative to the 47S rRNA initiation site (BK000964v3 ...
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bioRxiv - Genetics 2019Quote: ... A DNA fragment corresponding to the 700 bp upstream region of the PaPKS1 ORF was amplified with oligonucleotides 5’ tcgccgcggGCTAGGGGGTACTGATGGG 3’ and 5’ cacgcggccgcCTTGGAAGCCTGTTGACGG 3’ (capital letters correspond to P. anserina genomic DNA sequences) and cloned in SKpBluescript vector (Stratagene) containing the nourseothricin-resistance gene Nat in the EcoRV site (vector named P1 ...
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bioRxiv - Bioengineering 2022Quote: ... approximately one-million cells were incubated in the FCM buffer with biotinylated ACE2 peptidomimetics (h-deface2 and p-deface2) labeled with Streptavidin-phycoerythrin (SA PE, Prozyme) at the indicated protein concentrations (for one hour at 4°C in the dark ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Then quantification of viral copy numbers was carried out in duplicate by quantitative PCR using a Mx3005 P Thermocycler (Agilent) as described by [49] ...
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bioRxiv - Physiology 2023Quote: ... The cells were transfected with 100 ng of NF-κB firefly luciferase reporter plasmid p(NF-κB)3-Luc (Stratagene) and 10 ng of Renilla luciferase reporter plasmid pRL-RSV (Promega) ...
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bioRxiv - Plant Biology 2023Quote: ... 5989-8310EN (Agilent G1676AA Agilent Fiehn GC/MS Metabolomics RTL Library User Guide. June 2008. Agilent P/N: G1676-90000, Agilent Fiehn GC/MS Metabolomics RTL Library Product Note ...
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bioRxiv - Biochemistry 2023Quote: ... Mutations in the pCEFL-HA-HaloTag-P-Rex1 WT construct were created by QuikChange II site-directed mutagenesis (Agilent 200523). All constructs were confirmed by sequencing and expression was tested by immunoblot.
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bioRxiv - Biophysics 2021Quote: ... The thermal variations were in the range 25-95°C in a stepwise increment of 1 °C/minute step and using a 96-well PCR plate (Agilent Technologies) sealed with optical quality sealing tape (Microseal® ‘B’ seal from BIO-RAD) ...
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bioRxiv - Plant Biology 2019Quote: ... 60 °C for 20 s and 72 °C for 15 s (Clontech SYBR Green Master Mix and Mx3000P qPCR Systems, Agilent Technologies). Primers for circadian clock genes were designed using pcrEfficiency (Mallona et al. ...
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bioRxiv - Genetics 2023Quote: ... were fragmented at 60°C for 30min and hybridized to the microarrays for 17h at 65°C in a rotating hybridization oven (Agilent Technologies). After hybridization ...
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bioRxiv - Microbiology 2024Quote: ... 40x 10 sec 95°C and 30 sec 60°C with the Brilliant III Ultra-Fast qRT-PCR master mix (Agilent, 600884) were used according to manufacturer’s protocol.
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bioRxiv - Physiology 2024Quote: ... were subjected to quantitative PCR under a temperature profile of 95°C for 3 min followed by 40 cycles for 95°C for 5 sec and 58°C for 15 sec using the Stratagene Mx3000P (Agilent Technologies). For each of the samples ...
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bioRxiv - Molecular Biology 2024Quote: ... 30 s at 58 °C and 30 s at 72 °C were carried out on a Multiplex 3005 Plus (Stratagene/Agilent). The amplicon coordinates relative to the 47S rRNA initiation site (BK000964v3 ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein Block Serum Free reagent (Dako). Primary antibody incubation was performed at 4°C overnight using the ideal dilution for each antibody (Supplementary Table 5) ...
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bioRxiv - Cell Biology 2021Quote: ... and incubated in Protein Block (Dako) for 5 min ...
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bioRxiv - Cancer Biology 2021Quote: ... and protein (Dako cat. no. X0909) blocking reagents for 5 minutes each ...
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bioRxiv - Microbiology 2023Quote: ... AdvanceBio SEC 300Å protein standard (Agilent) was included to correlate the elution volume with molecular weight.
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bioRxiv - Microbiology 2023Quote: ... diluted with protein block buffer (Dako) for 2 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... protein blocker (Agilent Technology, X090930-2). Slides were incubated with a biotinylated anti-pimonidazole mouse IgG1 monoclonal antibody diluted 1:50 in Background Sniper (Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Dako Protein Block (Agilent Cat#X0909), ‘Normal’ block (Agilent Cat#S202386-2) ...
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bioRxiv - Biochemistry 2021Quote: ... at 4°C overnight diluted in antibody diluent (Dako). Negative controls were incubated with antibody diluent only ...
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bioRxiv - Immunology 2021Quote: ... DMEM (Agilent Cat# 102353, pH=7.4 at 37°C), supplemented with 143 mM NaCl and 2mM L-Glutamine for 1 hr ...
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bioRxiv - Bioengineering 2022Quote: ... and a PLgel 5μm MIXED-C column (Agilent Technologies) with chloroform eluent (1 mL/min) ...
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bioRxiv - Bioengineering 2022Quote: ... and a PLgel 5μm MIXED-C column (Agilent Technologies) with chloroform eluent (1 mL/min) ...
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bioRxiv - Synthetic Biology 2022Quote: ... and cooled to 37 °C (Agilent, Catalog #5190-9527). Eighteen replicate transformations of the cloned library were conducted using Agilent’s ElectroTen-Blue cells (Catalog #200159 ...
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bioRxiv - Cancer Biology 2022Quote: ... for 20 min at 97°C (PT-Link, DAKO). Endogenous peroxidase activity was quenched by incubation in peroxidase blocking buffer (DAKO ...
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bioRxiv - Cancer Biology 2022Quote: ... cytokeratin (DAKO, Cat. M3515; 1:100 at 4 °C), ki67 (ThermoFisher ...
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bioRxiv - Biochemistry 2023Quote: ... 66.3°C) in a PCR machine (Agilent SureCycler 8800) followed by 3 minutes at room temperature ...
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bioRxiv - Genomics 2023Quote: ... were used to assess the Hi-C libraries (Agilent). The libraries were sequenced at New York Genome Center (NYGC ...
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bioRxiv - Cancer Biology 2019Quote: ... for 15 min and subsequently replaced with anti-249T-P pAbs diluted at 1:250 with antibody diluent solution (Agilent Technologies). Slides were incubated at 4°C overnight and then washed and visualized with DAB+ substrate chromogen (Agilent Technologies) ...
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bioRxiv - Immunology 2022Quote: ... The resulting cDNA (equivalent to 500ng of total RNA) was amplified using the SYBR Green real-time PCR kit and detected on a Stratagene Mx3005 P (Agilent Technologies). qPCR was conducted using forward and reverse primers (sequences available upon request) ...
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bioRxiv - Cell Biology 2021Quote: ... was then mutated to either R to mimic deacetylated c-Myc (K323R) or Q to mimic acetylated c-Myc (K323Q) using QuickChange II Site-Directed Mutagenesis Kit (Agilent Technologies, 200522-5) against pPHAGE-EF1α-HA-Puro-c-Myc ...
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bioRxiv - Cell Biology 2023Quote: ... Site-directed mutagenesis was performed on the pGAMA-YAP construct to create p-GAMA-YAP-S127A using the QuikChange Lightning kit (Agilent Technologies #210513). Forward primers used for the Ser-to-Ala substitution was as follows ...
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bioRxiv - Microbiology 2020Quote: ... sealed with adhesive and incubated for 10 min at 65°C and overnight at 37°C on a FISH-hybridizer (Dako; Agilent Technologies, Santa Clara, CA). The slides were then immersed in a series of baths with SSC buffer at different concentrations of 4× ...
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bioRxiv - Microbiology 2019Quote: ... the microarray was analysed using the Microarray C Scanner (Agilent Technologies ...
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bioRxiv - Biochemistry 2022Quote: ... Zorbax 300 SB-C column from Agilent (Palo Alto, CA) and HALO ES column from Advanced Materials Technology (Wilmington ...
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bioRxiv - Cancer Biology 2022Quote: ... Glyko® Sialidase C™ was from Prozyme (Hayward, CA). All solutions were made with ultrapure Milli-Q water (Millipore ...
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bioRxiv - Neuroscience 2022Quote: ... using specific software of data analysis for GC from Agilent (OpenLAB CDS ChemStation v. C.01.10; Agilent Technologies, Barcelona, Spain) and subsequent expert’s revision and confirmation ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were further cleaned by C-18 resin columns (Agilent). (Cleanup and elution of peptides via C-18 column was performed as per the company protocols) ...