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Citations for Agilent :
401 - 450 of 1150 citations for Borrelia burgdorferi C p Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... Promoter capture Hi-C was performed using a custom-made RNA capture bait system (Agilent Technologies) consisting of 39,021 individual biotinylated RNAs targeting the ends of 22,225 promoter-containing mouse HindIII restriction fragments ...
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bioRxiv - Cell Biology 2022Quote: ... samples were exposed to antigen retrieval (10 minutes at 105°C in 1 x TRS (DAKO)) ...
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bioRxiv - Microbiology 2023Quote: ... with agitation at 37°C for 16 hours in an imaging multimode reader (Cytation 5, Agilent). Optical density at 600 nm (OD600) ...
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bioRxiv - Neuroscience 2023Quote: ... Partial purification was achieved using C-18 solid phase extraction columns (Agilent Technologies, Lake Forest, CA). A series of 4 elutions with 1.5 mL of 60% ...
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bioRxiv - Neuroscience 2023Quote: ... Mutation of Syngap1 (SYNGAP1-c.2214_2217del) was introduced by site-directed mutagenesis (QuikChange Lightning, Agilent #210518). HEK293T cells were co-transfected with expression vectors using Lipofectamin 3000 (ThermoFisher) ...
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bioRxiv - Immunology 2024Quote: ... at 4 °C ON and afterward incubated with rabbit anti-HA antibody 1:2000 (Dako, A0001) 2 h at RT ...
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bioRxiv - Cell Biology 2019Quote: ... cells were pre-incubated with blocking buffer (Protein Block Serum Free solution, DAKO) for 30 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit anti-glial fibrillary acidic protein (GFAP; Z0334, Dako, 1:20000/1:100000), rabbit anti-glutamine synthetase (GS ...
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bioRxiv - Genomics 2020Quote: ... The proteins were reduced with 5 mM DTT (Agilent Technologies Cat# 5188-6439), alkylated with 18 mM 2-chloroacetamide (Sigma-Aldrich Cat# C0267-100G ...
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bioRxiv - Cell Biology 2021Quote: ... The mGli2-His recombinant protein was expressed in BL21 (DE3) pLysS cells (Stratagene) and purified under denaturing condition (8 M urea ...
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bioRxiv - Cell Biology 2021Quote: ... placed in the humid chamber and incubated with Ultravision Protein-block solution (DAKO) for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein-coding genes were captured using SureSelectXT Human All Exon V5 probes (Agilent) and sequenced on Illumina HiSeq 4000 using 100bp paired end read protocol ...
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bioRxiv - Biochemistry 2022Quote: ... Protein concentrations were determined spectrophotometrically on a Cary 60 UV-visible spectrophotometer (Agilent) using calculated molar extinction coefficients at 280 nm ...
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bioRxiv - Molecular Biology 2022Quote: ... Slides were blocked with DAKO Protein Block Serum-Free (DAKO, Catalog No. X0909) for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... and manufacturer’s instructions followed for protein binding and elution (Agilent, Santa Clara, CA). The low abundance protein fraction was further fractionated by 1D SDS PAGE and analyzed using LC-MS/MS ...
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bioRxiv - Biochemistry 2021Quote: ... Digested protein (100μg) was loaded using high-performance liquid chromatography (Agilent 1260 uHPLC). Mobile phase was ...
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bioRxiv - Physiology 2019Quote: ... Sections were blocked with 2% normal donkey serum in protein block buffer (Dako) for 15 minutes and then incubated overnight at 4°C with primary antibodies diluted in DAKO antibody diluent ...
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bioRxiv - Molecular Biology 2020Quote: ... 200µg digested protein was loaded using high Performance Liquid Chromatography (Agilent 1260 uHPLC). Mobile phase was ...
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bioRxiv - Molecular Biology 2020Quote: ... After treatment with a Protein Block Serum-Free blocking reagent (DAKO, Code X0909) at room temperature for 30 min ...
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bioRxiv - Cancer Biology 2020Quote: ... The tissue sections were then blocked with serum-free protein blocking agent (Dako) and incubated with rabbit mAb for Phospho-Histone H2A.X (Ser139 ...
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bioRxiv - Bioengineering 2020Quote: ... rabbit anti-Glial fibrillary acidic protein (GFAP; 1:1000, Dako, Cat# Z033429, RRID:AB_10013382), mouse anti-CD68 (ED1 ...
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bioRxiv - Cell Biology 2021Quote: ... permeabilised (0.1% TritonX-100 in PBS) and blocked (Serum free protein block, DAKO), before overnight incubation with a rabbit polyclonal antibody to mouse CD31 (ab28364 ...
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bioRxiv - Neuroscience 2021Quote: ... Target protein was further purified by a 300SB-C3 RP-HPLC column (Agilent) with a linear gradient of acetonitrile (0-100% ...
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bioRxiv - Microbiology 2021Quote: ... For digestion of proteins in the insoluble fraction 20 µl StrataClean beads (Agilent), which bound 20 µg protein ...
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bioRxiv - Microbiology 2022Quote: ... Missense mutations in recombinant proteins were generated by QuikChange site-directed mutagenesis (Agilent) using the respective pET28b derivatives as templates ...
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bioRxiv - Neuroscience 2022Quote: ... Ki67 (1:1000) and glial fibrillary acidic protein (GFAP) (1:1500) from Dako Cytomation (Glostrup ...
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bioRxiv - Cell Biology 2023Quote: ... they were exposed to Protein Block Serum-Free Ready-To-Use (DAKO, Denmark) for 30 minutes at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... Protein unfolding profiles were recorded using an MX3005P real-time qPCR instrument (Agilent; excitation/emission filters = 492/610 nm ...
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bioRxiv - Cancer Biology 2023Quote: ... Endogenous peroxidases and non-specific signal (Flex Peroxidase Block and Protein Block, Dako) were blocked at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant proteins were expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells (Agilent) in LB medium at 16 °C overnight and protein expression was induced by 0.25 mM IPTG (final concentration ...
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bioRxiv - Physiology 2023Quote: ... Tissues were blocked with either serum free protein blocking reagent (Dako Agilent X0909) or 5% BSA ...
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bioRxiv - Physiology 2023Quote: ... Tissues were blocked with either serum free protein blocking reagent (Dako Agilent X0909) or 5% BSA ...
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bioRxiv - Cancer Biology 2023Quote: Urea in-solution protein digestion was performed on the AssayMAP Bravo (Agilent Technologies) micro-chromatography platform using the digestion v2.0 protocol ...
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bioRxiv - Physiology 2023Quote: ... blocked with a serum-free Protein blocking solution (DAKO, Los Altos, CA, USA) for 30 minutes and rinsed three times with PBST solution ...
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bioRxiv - Microbiology 2024Quote: ... rabbit glial fibrillary acidic protein antibody (anti-GFAP,1:500; Dako, Glostrup, Denmark), goat ionized calcium binding adaptor molecule 1 antibody (anti-Iba1,1:500 ...
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bioRxiv - Microbiology 2024Quote: ... and mouse myelin basic protein antibody (anti-MBP, 1:500, Dako, Glostrup, Denmark), respectively for 24 hours at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... encoding the desired protein constructs were transfected into Escheria coli BL21 (DE3) (Stratagene). At an OD660 of 0.8 ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentrations were determined spectrophotometrically on a Cary 60 UV-visible spectrophotometer (Agilent) using calculated molar extinction coefficients at 280 nm.
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bioRxiv - Neuroscience 2024Quote: ... Polyclonal Rabbit Anti-Glial Fibrillary Acidic Protein (Dako, Denmark, Z0334, dilution 1:200), Rabbit Anti-Iba1(Fujifilm ...
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bioRxiv - Synthetic Biology 2021Quote: ... The supernatant was removed and analyzed by LC-QQQ using MRM in negative ionization mode on an EclipsePlus C-18 RRHD column (1.8 µm, 2.1 × 50 mm, r.t, Agilent) or a Poroshell 120 SB-Aq column (2.7 µm ...
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bioRxiv - Plant Biology 2020Quote: ... a 6x Histidine tag was appended to the C-terminus of rbcS via the Quikchange protocol (Stratagene). Restriction free cloning of the RBS-AtRbcLScHis cassette was utilized to insert the cassette into the multiple cloning site 1 of the pRSFDuet™-1 plasmid (Novagen) ...
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bioRxiv - Microbiology 2019Quote: ... The sub-fraction was further purified by semipreparative HPLC with C-18 column (9.4 mm × 250 mm, 5.0µm, Agilent) using an acetonitrile/water gradient (0.1% formic acid ...
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bioRxiv - Microbiology 2020Quote: ... C-terminal flag tag (DYKDDDDK) was added to pHH21-NA encoding plasmids via site directed mutagenesis (Agilent). cDNA was generated from the pHH21-NA flag plasmids with Q5 Hot-Start PCR (NEB) ...
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bioRxiv - Biophysics 2020Quote: Samples were thawed and injected onto a cooled (2 °C) HPLC System (Agilent Infinity 1260, Agilent Technologies) equipped with a home packed pepsin column (IDEX guard column with an internal volume of 60 µL ...
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bioRxiv - Biophysics 2020Quote: Samples were thawed and injected onto a cooled (2 °C) HPLC System (Agilent Infinity 1260, Agilent Technologies) equipped with a home packed pepsin column (IDEX guard column with an internal volume of 60 µL ...
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bioRxiv - Neuroscience 2022Quote: ... They were then incubated overnight at +4 °C with the 6F3D anti-Aβ antibody (Dako, 1/200), polyclonal anti-tau antibody (Dako ...
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bioRxiv - Microbiology 2019Quote: Oxygen consumption rate (OCR) was measured at 37 °C using Seahorse XFp extracellular flux analyser (Seahorse Bioscience). XF cell culture microplate plates were coated with 10 μl of Cell-Tak (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2021Quote: ... Collected volatiles were thermally desorbed for 3 min at 220°C and analyzed by GC-MS (Agilent 7820A GC interfaced with and Agilent 5977E MSD ...
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bioRxiv - Developmental Biology 2019Quote: ... was applied at 4°C overnight and secondary polyclonal goat anti-mouse-HRP (Agilent P0447; 1:1,000) was applied for 1 h at room temperature ...
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bioRxiv - Plant Biology 2019Quote: ... Root length was measured 24h upon UV-C exposure (900 J/m2) using the Stratalinker 2400 (Stratagene). The relative root growth was calculated ...