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Citations for Agilent :
8451 - 8500 of 8609 citations for Cyclic GMP cGMP ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... the co-cultures were incubated first with primary antibody for glial fibrillary acidic protein (GFAP, 1:400, Z033429-2, Dako, Glostrup, Denmark) prepared in 5% NGS in DPBS overnight at +4 following an incubation with Alexa Fluor405 (A31556 ...
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bioRxiv - Physiology 2021Quote: ... Primary antibodies are diluted in 1x blocking solution and incubated with antibodies against insulin (1:2000, guineapig, Dako, cat. no. A-0546), glucagon (1:500 ...
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bioRxiv - Developmental Biology 2020Quote: ... and incubated for 30 minutes at room temperature with the secondary antibody (Supplementary Table 2)(1:500 diluted in DAKO Antibody Diluent). Cells were washed with 0.2% Triton X-100 in PBS ...
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bioRxiv - Microbiology 2021Quote: ... 190 to 601 nm (steps: 1 nm) and a Q-TOF (tandem mass spectrometry, quadrupole and Time of flight detector combined) (Agilent, Waldbronn, Germany). Electrospray ionization was performed in positive mode (ESI+ ...
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bioRxiv - Immunology 2021Quote: ... The membranes were washed and incubated with rabbit anti-goat IgG (500 ng ml−1, 5% milk in PBST; Dako, P044901-2), rabbit anti-mouse IgG (1.3 μg ml−1 ...
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bioRxiv - Microbiology 2021Quote: ... membranes were washed with 0.1% Tween-20 in PBS and incubated with anti-mouse or anti-human IgG (1:5000; Agilent Dako, Glostrup, Denmark), conjugated to horseradish peroxidase ...
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bioRxiv - Microbiology 2021Quote: ... were designed based on the DNA sequence for SARS-CoV-2 Wuhan-Hu-1 using the QuickChange Primer Design tool (Agilent Technologies, Inc.). Mutagenesis was carried out on a pCDNA-SARs2 Wuhan-Hu 1 S plasmid to create the P681H mutation ...
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bioRxiv - Immunology 2021Quote: ... and a 1:10 dilution of the resulting cDNA was run on a Bioanalyzer High Sensitivity DNA chip (Agilent Technologies #5067-4626) to assess cDNA quality and yield ...
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bioRxiv - Immunology 2021Quote: ... post-amplification and a 1:10 dilution of the resulting HTO libraries were run on a Fragment Analyzer (Agilent Technologies #5067-4626) to assess HTO quality and yield ...
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bioRxiv - Cell Biology 2021Quote: ... and deparaffinized prior to antigen retrieval in 0.1M sodium citrate pH6 and staining with guinea pig anti-insulin antibody (Dako A0564, 1:250) overnight at 4 degrees in the dark and visualized with Anti-Guinea Pig biotin conjugated antibody (Vectastain BA7000 ...
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bioRxiv - Developmental Biology 2022Quote: ... The PVDF membranes were blocked in 5 % nonfat dry milk diluted in Tris-buffered saline with 0.05 % Tween-20 for 1 h at room temperature and then incubated overnight at 4 °C with antibodies against FN (1:70000, rabbit anti-fibronectin, DAKO, Hamburg, Germany). After washing with TBS-T ...
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bioRxiv - Biophysics 2022Quote: Fluorescence titrations were carried out at 25 °C using 1 × 0.3 cm path-length cuvettes in a Cary Eclipse spectrofluorimeter (Agilent Technologies, Waldbronn, Germany). Experiments were carried out in 20 mM K-phosphate ...
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bioRxiv - Immunology 2022Quote: ... post-amplification and a 1:10 dilution of the resulting HTO libraries were run on a Fragment Analyzer (Agilent Technologies #5067-4626) to assess HTO quality and yield ...
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bioRxiv - Molecular Biology 2022Quote: ... Adapter dilution (1:4) and PCR amplification (26 cycles) were optimized in a pilot experiment using Bioanalyzer DNA1000 (Agilent, Sant Clara, USA) chips as a read out of library size and quantity ...
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bioRxiv - Molecular Biology 2022Quote: ... at 37°C for 1 hour followed by vehicle or TGF-β (5 ng/ml) and 3000 cells were seeded into Seahorse assay microplates (Agilent Technologies). After 24h of incubation ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were then incubated for 1 hour at room temperature in the HRP-labelled polyclonal goat anti-mouse IgG (DAKO, cat. P0447) or HRP-labelled polyclonal goat anti-rabbit IgG (DAKO ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were then incubated for 1 hour at room temperature in the HRP-labelled polyclonal goat anti-rabbit IgG (DAKO, cat. P0448) or HRP-labelled polyclonal goat anti-mouse IgG (DAKO ...
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bioRxiv - Cancer Biology 2024Quote: ... at 1:400 for two hours and then incubated for 30 minutes with Rabbit Envision HRP System reagent (Agilent, Santa Clara, CA). For vimentin staining ...
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bioRxiv - Molecular Biology 2024Quote: ... then spectrometry readings were collected every 5 min for 1 h using a BioTek Epoch microplate spectrophotometer (Agilent Technologies, Santa Clara, CA).
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bioRxiv - Immunology 2024Quote: ... The final PCR products were purified by AMPure XP beads at a 0.8:1 bead to sample ratio and quality controlled by TapeStation (D1000, Agilent, cat# 5067-5584). The samples were pooled at equimolar concentrations and further purified by gel extraction and AMPure XP bead clean up.
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bioRxiv - Neuroscience 2024Quote: OCR assay: Cells were washed and incubated for 1 hour with 180 μL assay medium [in mmol/L: 5 glucose (Agilent #103577-100), 1 pyruvate (Agilent #103578-100) ...
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bioRxiv - Plant Biology 2024Quote: ... 1:500 cDNA dilutions were analyzed using Brilliant III Ultra-Fast SYBR Green QPCR Master Mix (Agilent Technologies, Santa Clara, CA, USA), required primer pairs (Table S2) ...
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bioRxiv - Cancer Biology 2023Quote: ... depending on the antibody used (HIF-1α, Dako Target Retrieval Solution pH 6; for BACH-1, Dako Target Retrieval Solution pH 9). Next ...
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bioRxiv - Synthetic Biology 2023Quote: ... 100 μL of the ethyl acetate phase was transferred into a GC vial with insert and 1 μL was analyzed using GC 8890 (Agilent Technologies, USA) equipped with a flame ionization detector (FID ...
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bioRxiv - Synthetic Biology 2023Quote: ... composed of 1 ng of the cDNA library quantified using TapeStation with High Sensitivity D5000 ScreenTape (Agilent #5067-559 and #5067-5593), 0.7 µL each of 20 µM forward primer P5-TSO_Hybrid (45 ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were loaded onto a ZORBAX SB-C18 column (150 x 1 mm, 3.5 μm, flow of 90 μl/min; Agilent, Les Ulis, France) heated at 40°C ...
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Retinal affectation in Huntington’s disease mouse models concurs with a local innate immune responsebioRxiv - Neuroscience 2023Quote: ... and incubated overnight in a humid chamber at 4°C with rabbit anti-glial fibrillary acidic protein (GFAP) antibody (1:500, Z0334, DAKO, Agilent, Glostrup, Denmark), rabbit anti-ionized calcium binding adaptor molecule 1 (Iba-1 ...
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bioRxiv - Biochemistry 2022Quote: ... The metabolite-containing supernatant (195 µL) was transferred into pre-cut Bond® Elut PH (100 mg, 1 ml) SPE cartridges (Agilent, 12102005) placed in 1.7 mL microcentrifuge tubes and then centrifuged at ∼3000 g for 3 min ...
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bioRxiv - Immunology 2023Quote: ... An anti-rabbit IgG horseradish-peroxidase conjugated (1/2000 dilution) was used as a secondary antibody (Agilent DAKO, Santa Clara, CA, USA) and 4-Chloro-1-Naphthol as substrate for colorimetric detection.
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bioRxiv - Microbiology 2023Quote: ... Fluorescence was measured at 50°C over 1 h at excitation 360 nm emission 460 nm on a BioTek Synergy HTX Multimode Reader (BioTek, Agilent, Tx, USA). The reaction rate was calculated as the maximum velocity over 12 sequential readings ...
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bioRxiv - Neuroscience 2023Quote: ... sections were counterstained with DAPI (1:1000 in 1X PBS) for 5 min and coverslipped with fluorescent mounting medium (#S3023, DAKO, Jena, Germany). Images were obtained using an Axioplan M2 fluorescent microscope (Zeiss ...
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bioRxiv - Physiology 2023Quote: ... For β-cell proliferation analysis in P1 pups every other section was labeled with guinea pig anti-insulin (1:200; DAKO A0564) and mouse anti- BrdU (1:500 Sigma B2531 ...
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bioRxiv - Developmental Biology 2023Quote: ... membranes were incubated for 1 h at room temperature with polyclonal rabbit anti-mouse secondary antibody IgG/HRP (P0260; Agilent Technologies/Dako) diluted 1:2000 in 3% BSA.
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bioRxiv - Microbiology 2023Quote: ... fitted with a flame photometric detector (FPD) and a J&W DB-1 column (30 m x 0.32 mm Ø; Agilent Technologies, USA). The oven temperature was 180°C ...
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Mass spectrometric analysis and biostimulatory effects of boar seminal gel, saliva and semen in pigsbioRxiv - Animal Behavior and Cognition 2023Quote: ... An aliquot of 1 μL was injected into GC-MS (GC 9000 and MS of G7077B, Agilent Technologies, Palo Alto, CA, USA) for further analysis ...
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bioRxiv - Immunology 2023Quote: ... An anti-rabbit IgG horseradish-peroxidase conjugated (1/2000 dilution) was used as a secondary antibody (Agilent DAKO, Santa Clara, CA, USA) and 4-Chloro-1-Naphthol as substrate for colorimetric detection.
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed four times for 5 min each in TBST and incubated with Polyclonal goat anti-mouse (P044701, Agilent, 1:2500) or anti-rabbit (P044801 ...
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bioRxiv - Microbiology 2023Quote: ... Gas samples from the headspaces (250 μl) were collected using a 1 mL Hamilton’s SampleLock™ syringe and manually injected into a Gas Chromatograph (GC) (Agilent 7820A, AgilentTechnologies). H2 and O2 were separated using a packed column (60/80 Molecular Sieve 5A ...
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bioRxiv - Cell Biology 2024Quote: Protein expression was initiated by transforming ∼80 ng of each DNA plasmid into 50 µL of XL-1 Chemically Supercompetent Cells (Agilent Technologies, 200236) at 42 °C for 30 seconds followed by 5 minutes of incubation on ice ...
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bioRxiv - Systems Biology 2024Quote: ... and detected by 1:2000 dilution of respective secondary HRP-antibody-conjugates (anti-rabbit, P044801-2, Agilent; anti-mouse, P044701-2, Dako).
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bioRxiv - Genomics 2023Quote: ... The average library size was obtained via analysis of 1 μL of the library on the Agilent TapeStation 2200 (Agilent, Cat # G2964AA) using D1000 screen tape (Agilent ...
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bioRxiv - Neuroscience 2024Quote: ... A total of 1 × 105 cells per well were seeded on a 24-well Xfe96 Cell culture Microplate (Agilent, Cat# 100777-004) coated with poly-L-lysine (Millipore Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... Wildtype mouse brain paraffin sections were used for IHC analysis with a dilution of mouse serum at 1:200 with antibody diluent solution (DAKO, S080983-2). Mouse anti-NMDAR1 monoclonal antibody (BD ...
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bioRxiv - Pathology 2024Quote: ... One hour of shaking incubation at 30℃ resulted in the extraction of 1 mL of gas for ethylene measurement using gas chromatography (Agilent 7820A, USA).
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated for 90 minutes in a biotin conjugated secondary antibody (a goat anti-rabbit, a goat anti-mouse or a swine anti-rabbit as appropriate; Dako; diluted 1:500) and for 1h in a peroxidase conjugated extravidin (Sigma ...
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bioRxiv - Immunology 2020Quote: ... Purified cDNA was subjected to quality control using 1 µl of cDNA on an Agilent 2100 BioAnalyser (Agilent Technologies, Santa Clara, CA, USA) using the Agilent High Sensitivity DNA kit ...
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bioRxiv - Molecular Biology 2019Quote: ... concentration and purity were evaluated on an Agilent RNA6000 Nano Chip in Reagent Port 1 of the Bioanalyzer Agilent 2100 (Agilent Technologies, CA, USA). Sequencing of the libraries was done on the Illumina HiSeq 4000 platform by BGI Co. ...
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bioRxiv - Bioengineering 2019Quote: ... The following primary antibodies were used for immunocytochemistry experiments: rabbit anti-glial fibrillary acidic protein (GFAP) (DAKO, 1:100, Z-0334, Lot #2002332), mouse anti-vascular endothelial (VE)-cadherin (Abcam ...
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bioRxiv - Cell Biology 2019Quote: ... and then probed with primary antibody (see Supp. Table 2) followed by the appropriate HRP-conjugated secondary antibody (1:5000) (Agilent, Santa Clara, CA).
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bioRxiv - Neuroscience 2019Quote: ... and rabbit anti-S100 calcium-binding protein B β-subunit (S100-β) polyclonal antibody (Agilent Dako, Santa Clara, CA, USA Z0311, 1:400). Following washing ...